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Registros recuperados: 21
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Albendazole Sulphoxide Administered Prior to Mating andits Relation with Fertilization and Mouse Embryo Development International Journal of Morphology
Teruel,M; Catalano,R; Salomón,L.
The aim of this work was to evaluate the effect of albendazole sulphoxide (ABZSO) administered to Balb C mice prior to mating on fertilization rate and preimplantational embryo development. Twenty four female mice 5-8 weeks of age were superovulated by intraperitoneal injection of 7.5 UI of equine chorionic gonadotropin (eCG, Novormon®, Laboratorios Syntex S.A., Argentina); 48 h later they received 10 IU of human chorionic gonadotropin (hCG, Profasi®, Laboratorios Serono, Méjico) and were paired with males of proven fertility. Females received 100 mg/kg or 200 mg/kg of ABZSO orally at the time of hCG administration, prior to mating. The control group received carboxymethylcellulose, vehicle used to prepare the drug suspension. Pregnant females were killed...
Tipo: Journal article Palavras-chave: Albendazole Sulphoxide; Reproductive toxicity; Embryo development; Mouse.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300024
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Biochemical composition, tissue origin and functional properties of egg perivitellins from Pomacea canaliculata Biocell
Dreon,Marcos S.; Heras,Horacio; Pollero,Ricardo J..
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apple snails; Lipoproteins; Glycoproteins; Carotenoproteins; Embryo development; Antioxidant compounds; Light-protecting compounds.
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200013
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Challenges and perspectives to enhance cattle production via in vitro techniques: focus on epigenetics and cell-secreted vesicles Ciência Rural
Bressan,Fabiana Fernandes; Fantinato-Neto,Paulo; Andrade,Gabriella Mamede; Sangalli,Juliano Rodrigues; Sampaio,Rafael Vilar; Silveira,Juliano Coelho da; Perecin,Felipe; Meirelles,Flávio Vieira.
This review aim to present some clinical problems found in IVP-derived animals focusing on NT procedures and to discuss the possible role of epigenetics in such process. Also, as cell-secreted vesicles have been reported as possible regulators of important physiological reproductive processes such as folliculogenesis and fertilization, it is also presented herein a new perspective of manipulating the pre-implantation period trough effector molecules contained in such vesicles.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryo development; Nuclear transfer; Epigenetics; Extra-cellular vesicles..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001001879
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Differences between NMRI and DBA/2J mice in the development of somites and susceptibility to methylnitrosourea-induced skeleton anomalies Anais da ABC (AABC)
CHAHOUD,IBRAHIM; PAUMGARTTEM,FRANCISCO J.R..
ABSTRACT The development of DBA/2J mouse strain embryos is nearly 12 h - or 6 somite pairs - delayed as compared to the outbred NMRI mouse embryos of the same age on gestation days (GD) 8-12. To evaluate inter-strain differences in susceptibility to teratogens, dams were treated with methylnitrosourea (MNU, 5 mg/kg body weight i.p.) on defined gestation days (NMRI: GD 9, 91/2 or 10; DBA/2J: GD 10 or 101/2). Skeletal anomalies produced by MNU on both mouse strains varied with the GD of treatment. The pattern of anomalies produced by MNU on a given GD markedly differed between the two mouse strains, yet they were similar -with a few exceptions- when exposures at equivalent embryonic stages are compared. Findings from this study indicated that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkylating agents; Embryo development; Phase specificity; Mouse strains; Skeleton anomalies; Teratogenicity.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200635
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Efeitos do horário de postura de matrizes pesadas sobre o rendimento de incubação Arq. Bras. Med. Vet. Zootec.
Barbosa,V.M.; Rocha,J.S.R.; Pompeu,M.A.; Fernandes,M.N.S.; Machado,A.C.; Cunha,C.E.; Cardeal,P.C.; Ruiz,L.E.A.; Lara,L.J.C.; Baião,N.C..
The effects of oviposition time, which represents different positions of the follicles in a sequence of posture, were studied on egg weight, chick weight, egg weight loss during incubation, hatchability, embryo mortality and infertility . A total of 19, 820 eggs, layed on the same day, from Cobb® broiler breeders aging 46-week-old were used. The eggs were classified according to the oviposition time posture (corresponding to the time of collection) from 8:30 am, 10:30 am, 12:30 pm, 14:30 pm and 16:30 pm, which represented the folowing treatments: 1st, 2nd, 3rd, 4th and 5th collects, respectively. The experimental design was completely randomized with five treatments and 36 repetitions, and each tray of 96 eggs was considered a repetition. The normal and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broiler breeders; Embryo development; Incubation; Time of laying.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000400045
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Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro. Repositório Alice
CRUZ, M. H. C.; SARAIVA, N. Z.; CRUZ, J. F. da; OLIVEIRA, C. S.; COLLADO, M. del; FERNANDES, H.; CASTRO, F. C. de; GARCIA, J. M..
2014
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Embryo development; Inner cell mass; In vitro fertilization; Oocytes.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1010500
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Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro R. Bras. Zootec.
Cruz,Maria Helena Coelho; Saraiva,Naiara Zoccal; Cruz,Jurandir Ferreira da; Oliveira,Clara Slade; Del Collado,Maite; Fernandes,Hugo; Castro,Fernanda Cavallari de; Garcia,Joaquim Mansano.
This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryo development; Inner cell mass; In vitro fertilization; Oocytes.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000300003
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Effect of histone deacetylase inhibitor during pre-maturation and/or in vitro maturation of bovine oocytes on embryonic development. Repositório Alice
PONTELO, T.; KAWAMOTO, T.; CAIXETA, F.; ZANGERONIMO, M.; DODE, M. A. N..
bitstream/item/191133/1/v15n32018-Proccedings-SBTExAETE-275-275.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Embryo development.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1104628
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Effect of storage period and egg weight on embryo development and incubation results Rev. Bras. Ciênc. Avic.
Schmidt,GS; Figueiredo,EAP; Saatkamp,MG; Bomm,ER.
A total number of 300 females and 30 males of two experimental broiler lines, TT (male) and PP (female), with 39 and 36 weeks of age, respectively. Experiment 1 (TT) evaluated the effect of storage time (ST) on hatchability and embryo mortality (EM). Eggs were stored for 2 (ST1), 4 (ST2), 6 (ST3), 8 (ST4), 10 (ST5), 12 (ST6), and 24 (ST7) days and EM was assessed in the periods of 0 to 6 (EM1), 7 a 13 (EM2), 14 to 17 (EM3), 18 to 21 (EM4), and 0 to 21 (TEM) days. Experiment 2 (PP) evaluated the effect of egg weight (EW) on embryo development. Eggs were individually collected and weighed. Embryos were collected and weighed on days 9 (EW9), 11 (EW11), 13 (EW13), EW15, 17 (EW17) days of incubation and at hatching (EW21). ST linearly influenced hatchability...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chick weight; Broiler breeders; Embryo development; Embryo mortality; Egg weight; Incubation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000100001
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Effect of supplementation of green tea polyphenols on the developmental competence of bovine oocytes in vitro BJMBR
Wang,Z.G.; Yu,S.D.; Xu,Z.R..
The objective of the present study was to examine the effect of green tea polyphenols (GTPs) supplementation during in vitro maturation, in vitro fertilization, and in vitro culture on the developmental competence of bovine oocytes. Cumulus-oocyte complexes aspirated from the ovaries were matured in vitro (38.5ºC for 24 h) and fertilized (38.5ºC for 15-18 h) and embryos were cultured (38.5ºC for 192 h) in a defined conditioned medium with or without GTPs supplementation. The GTPs used in the present study contained 99% catechin derivatives, with the major components being 50% (-)-epigallocatechin gallate, 22% (-)-epicatechin gallate, 18% (-)-epigallocatechin, and 10% (-)-epicatechin. Four replicate trials were done for each type of experiment. GTPs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine oocytes; In vitro culture; Green tea polyphenols; Embryo development.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800008
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Effects of green tea polyphenols, insulin-like growth factor I and glucose on developmental competence of bovine oocytes R. Bras. Zootec.
Wang,Zhengguang; Fu,Chunquan; Yu,Songdong.
The present study examined the effects of green tea polyphenols (GTP), insulin-like growth factor-I (IGF-I) and glucose on oocyte in vitro maturation, subsequent embryo development and blastocyst quality in bovine. Cumulus-oocyte complexes (COC) were aspirated from the ovaries and cultured in synthetic oviduct fluid supplemented with MEM amino acids (SOFaa) media supplemented with one of the following supplements: GTP (0, 10, 15 and 20 µM), IGF-I (0, 50, 100 and 150 ng/mL) or glucose (0, 1.5, 5.6 and 20 mM) for 24 h. The results showed that oocytes cultured in media supplemented with 15 µM GTP, 100 ng/mL IGF-I and 5.6 mM glucose, in separate experiments, have higher cleavage and blastocyst rates compared with oocytes cultured in media without or with other...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blastocyst quality; Embryo development; In vitro culture; In vitro maturation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001200006
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Embryo development alteration in rats treated with lapachol BABT
Maganha,Juliana; Rocha,Evelise de Souza; Brandão,Marcos Antônio Fernandes; Peters,Vera Maria; Guerra,Martha de Oliveira.
Lapachol, a naphthoquinone extracted from plants of the genus Tabebuia (family Bignoneaceae), showed multiple therapeutic activities. Pregnant Wistar rats were treated with Lapachol from the 1st to the 4th (pre-implantation period) and from 5th to 7th (implantation period) post insemination day (PID). Mothers were sacrificed on the 5th or on the15th PID. Number of corpora lutea, preimplantation embryo, blastocysts, live and dead fetuses and resorptions were counted. There were no signs of maternal toxicity. The number and the morphology of embryos, during oviduct development (pre-implantation period), did not seem to be affected by this drug, but during the implantation period, lapachol was toxic causing the death of embryos and intrauterine growth...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lapachol; Embryo development; Rat; Reproductive toxicity.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700010
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Growth differentiation factor 9 and bone morphogenetic protein 15 expression in previtellogenic oocytes and during early embryonic development of Yellow-tail Kingfish Seriola lalandi Biol. Res.
Palomino,Jaime; Herrera,Giannina; Dettleff,Phillip; Martínez,Víctor.
BACKGROUND: During fish oocyte maturation, specific molecules are expressed and accumulated within oocyte until fertilization and embryo development. Special attention have been paid in members of the transforming growth factor (TGF-β) superfamily; growth differentiation factor 9 (GDF9/gdf9) and bone morphogenetic protein 15 (BMP15/bmp15), which exert regulatory functions during oocyte maturation and follicle development. However, little attention has been paid to the involvement of these molecules during embryogenesis considering its importance for the formation of a good quality egg and subsequent embryo survival. The purpose of this study was to analyze the expression of gdf9 andbmp15 in previtellogenic oocytes and during early embryonic...
Tipo: Journal article Palavras-chave: Gdf9; Bmp15; Embryo development; Oocyte maturation; Fish.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100054
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Histological processing techniques for the study of Dugesiidae development (Platyhelminthes, Tricladida, Paludicola) BJB
VARA,D. C. da; LEAL-ZANCHET,A. M.; LIZARDO-DAUDT,H. M..
The objective of the present study was to adapt techniques for the histological processing of Dugesiidae cocoons for the study of embryo development. The cocoons were fixed with formalin, SUSA, Bouin or paraformaldehyde/glutaraldehyde and subsequently embedded in Paraplast or glycol methacrylate (Historesin). Paraplast embedding yielded reasonable results only after the cocoon was perforated or fixed for a prolonged period of time using softening techniques with acid solutions. When the SUSA or Bouin fixative and Historesin embedding techniques were used the results were good for light microscopical analysis. Fixation with paraformaldehyde/glutaraldehyde and glycol methacrylate embedding resulted in better tissue preservation, and did not require prolonged...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryo development; Techniques; Glycol methacrilate; Tricladida.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082001000200019
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Influence of egg pre-storage heating period and storage length on incubation results Rev. Bras. Ciênc. Avic.
Silva,FHA; Faria,DE; Torres,KAA; Faria Filho,DE; Coelho,AAD; Savino,VJM.
This experiment aimed at evaluating the influence of different heating times of settable eggs of Cobb 500® broiler breeders before submitting them to different storage periods on egg weight loss, embryo mortality, and hatchability. A total number of 1,980 eggs were distributed in a completely randomized experimental design with a 3 x 3 factorial arrangement, comprising nine treatments with 22 replicates of 10 eggs each. The following factors were analyzed: pre-storage heating periods (0, 6, 12 hours at 36.92°C) and storage periods (4, 9, 14 days at 12.06°C). After storage, eggs were incubated under usual conditions, and were transferred to the hatcher at 442 hours of incubation. Eggs were weighed before heating, incubation, and transference to determine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broiler breeders; Egg weight loss; Embryo development; Embryo mortality; Hatch.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2008000100003
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Influence of egg pre-storage heating period and storage length on the digestive tract of newly-hatched broiler chicks Rev. Bras. Ciênc. Avic.
Silva,FHA; Faria,DE; Torres,KAA; Faria Filho,DE; Coelho,AAD; Savino,VJM.
An experiment was carried out to evaluate the effect of different heating times of settable eggs of Cobb 500® broiler breeders before submitting them to different storage periods on body weight, digestive tract organ weights, and intestinal mucosa morphology of newly-hatched chicks. Settable eggs were distributed in a completely randomized experimental design with a 3 x 3 factorial arrangement: pre-storage heating periods (0, 6, 12 hours at 36.92°C) and storage periods (4, 9, 14 days at 12.06°C). Body weight and relative weights of the yolk sac, heart, liver, proventriculus+gizzard, and intestinal segments were measured in chicks hatching at 480 and 498 hours of incubation. Villi height, width and perimeter, and crypt depth (ìm) were measured in duodenal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broilers; Embryo development; Incubation; Intestinal mucosa; Morphometrics.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2008000100004
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Modulation of murine blastocyst hatching in vitro by glutamine and tryptophan BJMBR
Castro,W.; Bueno Sánchez,J.C.; Piedrahita-Ochoa,C.; Cadavid J.,A.P..
Enrichment of culture media with amino acids improves embryo development. However, little is known about the specific action of each amino acid during embryogenesis. The present study was undertaken to examine the effect of L-glutamine (Gln) and tryptophan (Trp) on mouse embryo hatching, expansion and viability in vitro. Blastocysts were collected from 6- to 8-week-old female BALB/c mice (N = 30) and cultured in M2 medium containing either 0.125, 0.25 or 0.5 mM Trp, 1 mM Gln, or M2 alone. Gln significantly increased (100%; P < 0.05) blastocyst hatching at 24 h compared to M2 alone or Trp; moreover, Trp inhibited blastocyst hatching when compared to M2 alone (P < 0.05) at 72 h. In contrast, the percentage of embryos reaching the state of expanded...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryo development; Murine blastocyst; Hatching; Glutamine; Tryptophan.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800003
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Profile and regulation of annexin II expression during early embryogenesis in cattle Arq. Bras. Med. Vet. Zootec.
Costa,L.F.S.; Machado,M.S.N; Oliveira,J.F.C.; Silva,J.C.; Loguercio,R.S.; Gonçalves,P.B.D..
The presence of annexin II (Ann-II) during the initial stages of bovine embryo development and the regulation of Ann-II expression by retinol and insulin-like growth factor I (IGF-I) were studied. Bovine embryos at different stages of development were produced in vitro on Synthetic Oviductal Fluid (SOF) medium (control group), SOF supplemented with retinol (retinol group; 0.1ng/ml), or IGF-I (IGF-I group; 10ng/ml). The embryos were processed for mRNA extraction, cDNA production and polymerase chain reaction (PCR) using Ann-II-specific oligonucleotides. Ann-II was detected in all stages of early embryo development, except for the 16-cell stage. The blastocyst rates were significantly higher (P<0.05) in the group supplemented with retinol (37.8%, 45/119)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; Annexin II; Embryo development.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000600023
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Regulation of mouse embryo development by autocrine throphic factors Biocell
Teruel,M.T.; Catalano,R.C.; Cabodevila,J.A.; Callejas,S.S..
Embryo development depends on maternal and embryonic factors. When occurs in vitro, embryos secrete factors that stimulate their development. The purpose of this study was to investigate the possible effects of embryos at morula stage on mouse embryo development in vitro. To obtain conditioned media (CM), morulas were cultured in groups of 5 (CM5) or 10 (CM10) in microdrops of Ham-F10 culture medium during 24 h and later they were removed. Subsequently, 365 morulas were cultured in CM5 and CM10 or in Ham-F10 media (as control group). No differences in blastocyst formation could be found between embryos cultured for 24h in Ham-F10, CM5 or CM10 (49.66, 53.04, 60.00% respectively). However, CM5 significantly increased differentiation in embryos cultured for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryo development; Mouse; Autoregulation; Trophic factors.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200005
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The perturbed expression of m6A in parthenogenetic mouse embryos Genet. Mol. Biol.
Hao,Jindong; Xianfeng,Yu; Gao,Wei; Wei,Jiaqi; Qi,Minghui; Han,Liang; Shi,Shuming; Lin,Chao; Wang,Dongxu.
Abstract Parthenogenetically activated oocytes cannot develop to term in mammals owing to abnormal epigenetic modifications. Methylation of the N6 position of adenosine (m6A) is a post-transcriptional epigenetic modification of RNA. To investigate the role of m6A methylation in parthenogenetic (PA) embryonic development, we analyzed METTL3, METTL14, FTO, ALKBH5, YTHDF2, IGF2BP1, and IGF2BP2 expression by quantitative real-time PCR. These genes were found dynamically expressed during the 2-cell, 4-cell, 8-cell, and blastocyst stages of the embryo. Compared to normally fertilized embryos, the expression of these genes was perturbed in PA embryos, especially at the 8-cell stage. Furthermore, immunofluorescence was used to detect m6A expression. The results...
Tipo: Info:eu-repo/semantics/other Palavras-chave: M6A; Gene expression; Embryo development; Parthenogenetic.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400666
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