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Contrasting effects of heat shock during in vitro maturation on development of in vitro-fertilized and parthenogenetic bovine embryos. Repositório Alice
CAMARGO, L. S. A.; COSTA, F. Q.; MUNK, M.; WOHLRES-VIANA, S.; SERAPIÃO, R. V.; CARVALHO, B. C.; CAMPOS JÚNIOR, P. H.; VIEIRA, A. C.; NOGUEIRA, L. A. G.; VIANA, J. H. M..
bitstream/item/203452/1/Camargo-et-al-2019-Reproduction-in-Domestic-Animals.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Activation; Embryos; Maturation; Aquaporin 3; Hsp70 Protein; Ionomycin; Heat Shock; Cleavage; Blastocyst; In vitro fertilization; Parthenogenesis; Apoptosis; Gene expression; Gametocytes; Oocytes.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113387
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Contrasting effects of heat shock during in vitro maturation on development of in vitro-fertilized and parthenogenetic bovine embryos. Repositório Alice
CAMARGO, L. S. A.; COSTA, F. Q.; MUNK, M.; WOHLRES-VIANA, S.; SERAPIÃO, R. V.; CARVALHO, B. C.; CAMPOS JÚNIOR, P. H.; VIEIRA, A. C.; NOGUEIRA, L. A. G.; VIANA, J. H. M..
Abstract This study investigated the influence of heat shock during in vitro maturation on embryo development following in vitro fertilization (IVF) or parthenogenesis (Part). Immature bovine cumulus?oocyte complexes were exposed to heat shock (41.0°C) during the first 12 hr of in vitro maturation (IVM), followed by 12 hr at 38.5°C. Control group consisted of in vitro maturation for 24 hr at 38.5°C. Oocytes were in vitro&#8208;fertilized or activated with ionomycin and cultured in vitro for 192 hr post&#8208;in vitro insemination or parthenogenetic activation (hpia). There was an interaction (p < .01) between temperature of IVM and method of oocyte activation (IVF or Part) for cleav&#8208;age at 48 hpia. Heat shock had a negative impact (p...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Activation; Embryos; Maturation; Aquaporin 3; Hsp70 Protein; Ionomycin; Heat Shock; Cleavage; Blastocyst; In vitro fertilization; Parthenogenesis; Apoptosis; Gene expression; Gametocytes; Oocytes.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118469
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Development and germination of somatic embryos on sugar-free wheat starch medium in carrot (Daucus carota L. cv. Nantes Duke). Repositório Alice
FARI, M.; SORVARI, S.; AHANEN, K; TOLDI, O.; MELO, N. F. de; HIRSALMI, H..
bitstream/item/185576/1/DEVELOPMENT-AND-GERMINATION-OF-SOMATIC-EMBRYOS-ID-21682-p.128.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Desenvolvimento; Carrot; Embryos; Development; Biotecnologia; Cenoura; Daucus Carota; Embrião; Germinação; Biotechnology; Germination.
Ano: 1997 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/131985
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Diel changes in embryonic maturation in two species of marine cladocerans in Guanabara Bay, Rio de Janeiro, Brazil Zoologia
Marazzo,Andrea; Valentin,Jean L..
Two species of marine cladocerans, Penilia avirostris Dana, 1852 and Evadne tergestina Claus, 1877 were collected in thirteen samples at a fixed station in Guanabara Bay, Rio de Janeiro (30-meter depth) by means of vertical tows with a 200 µm mesh size net at different times from September 12th to 15th, 1995. The parthenogenetic females of Evadne tergestina bore mature embryos (with pigmented eye) only in the samples collected at night, whereas Penilia avirostris at any time of day. This phenomenon was interpreted based on the greater visibility of the Evadne tergestina brood: one of the benefits for embryo maturing during the darkness period would be a decrease of predation on gravid females.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cladocera; Penilia avirostris; Evadne tergestina; Zooplankton; Embryos; Guanabara Bay; Brazil.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000500033
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Distribution of microglial cells in the cerebral hemispheres of embryonic and neonatal chicks BJMBR
Ignácio,A.R.; Müller,Y.M.R.; Carvalho,M.S.L.; Nazari,E.M..
The distribution, morphology and morphometry of microglial cells in the chick cerebral hemispheres from embryonic day 4 (E4) to the first neonatal day (P1) were studied by histochemical labeling with a tomato (Lycopersicon esculentum) lectin. The histochemical analysis revealed lectin-reactive cells in the nervous parenchyma on day E4. Between E4 (5.7 ± 1.35 mm length) and E17 (8.25 ± 1.2 mm length), the lectin-reactive cells were identified as ameboid microglia and observed starting from the subventricular layer, distributed throughout the mantle layer and in the proximity of the blood vessels. After day E13, the lectin-reactive cells exhibited elongated forms with small branched processes, and were considered primitive ramified microglia. Later, between...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microglia; Cerebral hemispheres; Embryos; Neonatal chicks; Gallus domesticus; Tomato lectin.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100008
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Effects of cryoprotectant and plunging temperature in liquid nitrogen on the in vitro and in vivo development of murine morulae Braz. J. Vet. Res. Anim. Sci.
PANTANO,Thaís; MELLO,Marco Roberto Bourg de; GARCIA,José Fernando; HO,Linda Lee; VISINTIN,José Antonio.
The effects of plunging temperature in liquid nitrogen and cryoprotectant dilution methods were evaluated for compacted mouse morulae frozen in 1.5 M ethylene-glycol (E), 1.5M propylene-glycol (P) or 1.4M glycerol (G). Morulae were equilibrated for 10 minutes in cryoprotectant solution and loaded into 0.25 ml straws with cryoprotectant solution in 3 columns (groups E1, P1, G1) or cryoprotectant in the center and PBS in the lateral columns (E2, P2). Straws were cooled at 0.5ºC/min to -25 or -30ºC and plunged into liquid nitrogen. Straws were thawed in water at 22ºC for 20 seconds. Cryoprotectant was diluted in 3 steps for group G1 and in one step for groups E1 and P1 (direct transfer to PBS + 10% FCS) and E2 and P2 (shaken to mix the 3 columns before...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryos; Cryopreservation; Glycerol; Ethylene glycol; Propanediols.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000300012
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Endocrine profiles and ovulation rate of cows superovulated with FSH following passive immunization against steroid free-bovine follicular fluid Braz. J. Vet. Res. Anim. Sci.
ALVAREZ,Rafael Herrera; CARVALHO,João Batista Pereira de; ROSA E SILVA,Alzira; PERONE,Cibele Nunes; RIBELA,Maria Tereza Carvalho Pinto; OLIVEIRA FILHO,Enoch Borges de.
Ten multiparous non-lactating cows were randomly assigned to one of two groups. On days 8 to 12 after estrus, the first group was given 100 ml antiserum against steroid-free bovine follicular fluid (anti-bFF), which was produced in ovariectomized sheep. The second group (control) was given 100 ml nonimmune ovariectomized sheep serum. Six hours after injection, both groups were superovulated with FSH (18 NIH-FSH-S1 units) and LH (0.29 NIH-LH-S1 units) in a 4-day decreasing regime. On the morning of the third day a luteolytic dose of prostaglandin F2alpha analogue (cloprostenol) was given. Artificial inseminations (AI) were done 48 and 60 h later. Embryos were collected 7 days after AI by a nonsurgical method. Blood samples were taken throughout the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Superovulation; Follicular fluid; Passive immunization; Embryos; Cattle.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961998000600007
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Enzymatic polymorphism study as help for constitution of initial broodstock for a new cultivated finfish species. ArchiMer
Renno, J; Luquet, P.
Leporinus friderici is a South American promising species for fish culture. In French Guiana the "morphological species", L. friderici, L. granti, L. lebaili and L. affinis steyermarki are found. Their identification is sometimes difficult on usual criterion. To constitute an initial broodstock of L. friderici from local wild populations, the genetic structure of these species was studied. Fifteen enzyme systems, representing twenty loci, were screened.
Tipo: Text Palavras-chave: French Guiana; Leporinus; Leporinus granti; Leporinus friderici; Anostomidae; Pisces; Embryos; Mortality; Genetics; Freshwater fish; Rearing; Breeding; Biopolymorphism; Brood stocks; Enzymatic activity.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1441.pdf
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Evaluation of the Relationship Between the Breeder’s Age, Breed and the Incubator Types on Quality and Animal Welfare in the Hatchery Rev. Bras. Ciênc. Avic.
Oro,CS; De Souza,FEZ; Rodrigues,WC; Oro,JS; Guirro,ECBP.
ABSTRACT About 33.3% of chicken life is conditioned by the result of embryonic development. Therefore, understanding and improving the early phase of the broiler’s life is critical to guarantee their well-being. Thus, the objective of this research was to evaluate the relationship between the breeder’s age, breed and the incubator types on quality and animal welfare in the hatchery. The experimental design was completely randomized in a 2 x 2 x 3 factorial arrangement (incubator, breed and age), with 12 treatments and 6 replicates each. The eggs derived of Cobb 500 and Ross’s breeders at the beginning of the laying period, middle age and at the end of the laying period. It is concluded that the size of the eggs is related to the breeder’s age and breed,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryos; Hatch; Poultry.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300304
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Hematological values and body, heart and liver weights of male and female broiler embryos of young and old breeder eggs Rev. Bras. Ciênc. Avic.
Morita,VS; Boleli,IC; Cargnelutti Filho,A.
This study analyzed broiler breeder age (29 or 60 wk-old) effects on physical characteristics of eggs (initial mass, mass loss, volume, diameter, surface area and density) and of eggshells(weight, volume, thickness, conductance, and porosity ), as well as the influence of embryo sex on hematological parameters and body, liver and heart weights during incubation (at days 13, 15, 18 and 21). Physical parameter values were lower in 29-wk-old broiler breeder eggs than those of 60-wk-old breeders, except for relative eggshell weight, which was higher. In both male and female embryos, erythrocytic parameters and the body, liver, and heart weights increased during the incubation. The embryos and their organs were heavier when derived from 60-wk-old breeder eggs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood parameters; Breeder age; Embryos; Eggshell porosity and conductance; Sex.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000100002
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Importancia de los lípidos en la reproducción y ontogenia del pez blanco de Pátzcuaro (Chirostoma estor estor) OceanDocs
Aparicio, B..
Para producir larvas de peces con buenos rendimientos, se deben conocer los requerimientos nutricionales de la especie, particularmente en los primeros estadios larvarios. Además, se sabe que la nutrición de los reproductores influye directamente en la calidad de las larvas debido, principalmente, a la cantidad y calidad de lípidos que son transferidos desde la gónada hacia los huevos. El objetivo de este trabajo fue analizar la composición de lípidos en huevos, embriones y larvas de pez blanco de Pátzcuaro (Chirostoma estor estor) con el fin de establecer las bases de los requerimientos nutricionales de lípidos durante el desarrollo larvario del pez blanco. También se determinaron los ácidos grasos en tejidos de reproductores (cerebro, gónada, hígado,...
Tipo: Theses and Dissertations Palavras-chave: 1079; Lipids; Reproduction; Fish culture; Fish eggs; Embryos; Fish culture; Lipids; Reproduction; Http://aims.fao.org/aos/agrovoc/c_2918; Http://aims.fao.org/aos/agrovoc/c_4362; Http://aims.fao.org/aos/agrovoc/c_6507.
Ano: 2004 URL: http://hdl.handle.net/1834/3465
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Karyotype characterization, constitutive heterochromatin and nucleolus organizer regions of Paranaita opima (Coleoptera, Chrysomelidae, Alticinae) Genet. Mol. Biol.
Almeida,Mara Cristina de; Campaner,Carlos; Cella,Doralice Maria.
Species of the subtribe Oedionychina not only have a highly uniform diploid number of 2n = 22 (20+X+y) but have the karyotypic peculiarity of possessing extremely large sex chromosomes. We analyzed Paranaita opima embryos and gonadal cells to determine their diploid number, chromosomal morphology, type of sex determination system, constitutive heterochromatin pattern and which chromosomes bear nucleolus organizer regions (NORs). The diploid number of P. opima was 2n = 22 (20+XY/XX) with all chromosomes being metacentric. Chromosome pair 6 showed an interstitial secondary constriction on the short arm. The C-banding technique revealed centromeric constitutive heterochromatin in all chromosomes, which, in pair 6, extended up to the secondary constriction of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: C bands; Chromosome; Embryos; Fluorochromes; NOR.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300013
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La densite, un critere de qualite applicable en ecloserie aux embryos de Pecten maximus (mollusque bivalve) ArchiMer
Faure, Catherine; Devauchelle, Nicole; Dorange, Germaine.
The quality of the scallop embryos is determined by the spawners characteristics as well as by the external factors they support during the maturation and the spawning phases. All these parameters are not still well controlled during the artificial conditioning in the hatcheries. As a consequence, the quality of the embryos is drastically variable and with them, the larvae performances in terms of survival and growth rates. It would be interesting to get a simple and quick test which would allow the estimation of the spawns quality. The embryos density, evaluated by isopycnic centrifugation of Percoll medium, is well adapted to this objective. Actually, a centrifugation of embryos two hours after fertilization separates them in 3 stratum with their density...
Tipo: Text Palavras-chave: Pecten maximus; Bivalvia; Quality; Survival; Larvae; Growth; Sexual maturity; Spawning populations; Spawning; Embryos; Hatcheries.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-915.pdf
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Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos Scientia Agricola
Takada,Luciana; Martins Junior,Alicio; Mingoti,Gisele Zoccal; Balieiro,Julio César Carvalho; Coelho,Lia Alencar.
Melatonin (MEL) acts as a powerful scavenger of free radicals and direct gonadal responses to melatonin have been reported in the literature. Few studies, however, have evaluated the effect of MEL during in vitro maturation (IVM) on bovine embryos. This study tested the addition of MEL to maturation medium (MM) with no gonadotropins on nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos. Cumulus-oocyte complexes were aspirated from abattoir ovaries and cultured in MM (TCM-199 medium supplemented with 10% fetal calf serum - FCS) at 39ºC and 5% CO2 in air. After 24 hours of culture in MM with 0.5 µg mL-1 FSH and 5.0 µg mL-1 LH; 10-9 M MEL) or 10-9 M MEL, 0.5 µg mL-1 FSH and 5.0 µg mL-1 LH, the oocytes were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Comet assay; Embryos; Gonadotropins; In vitro fertilization; Melatonin.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000400003
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Nanoplastics impaired oyster free living stages, gametes and embryos ArchiMer
Tallec, Kevin; Huvet, Arnaud; Di Poi, Carole; Gonzalez-fernandez, Carmen; Lambert, Christophe; Petton, Bruno; Le Goic, Nelly; Berchel, Mathieu; Soudant, Philippe; Paul-pont, Ika.
In the marine environment, most bivalve species base their reproduction on external fertilization. Hence, gametes and young stages face many threats, including exposure to plastic wastes which represent more than 80% of the debris in the oceans. Recently, evidence has been produced on the presence of nanoplastics in oceans, thus motivating new studies of their impacts on marine life. Because no information is available about their environmental concentrations, we performed dose-response exposure experiments with polystyrene particles to assess the extent of micro/nanoplastic toxicity. Effects of polystyrene with different sizes and functionalization (plain 2-μm, 500-nm and 50-nm; COOH-50 nm and NH2-50 nm) were assessed on three key reproductive steps...
Tipo: Text Palavras-chave: Oyster; Embryos; Gametes; Microplastics; Nanoplastics.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00453/56419/58090.pdf
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Observaciones sobre embriones de larvas y juveniles de la pescadilla (Cynoscion striatus) OceanDocs
de Ciechomski, J.D.; Cassia, M.C..
Observations on the embrionic and larval development of Cynoscion striatus are presented. The description are made on the basis of material obtained by artificial fertilization. Eggs are pelagic, spherical, 700-840µ in diameter and with single oil globule of 210µ-240µ. The yolk is homogeneous and the chorion has smooth surface. The embryonic development is short and at temperatures of 19°-20°C hatching takes place, 34-36 hours after the fertilization. The larvae at hatching are small, about 2 mm in length and not advanced in their development. The mouth is not functional and the anus is not open. The oil globule is situated in the posterior part of the yolk sac; the position of the anus is close to the posterior end of the yolk sac and the digestice tract...
Tipo: Journal Contribution Palavras-chave: Embryos; Fish larvae; Fish larvae; Http://aims.fao.org/aos/agrovoc/c_2924.
Ano: 1982 URL: http://hdl.handle.net/1834/2045
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Parameters blood and viability oocyte Girolando's breed supplemented with linseed (Linum usitatissimum L.) - preliminary results. Repositório Alice
CARDOSO, C. J. T.; SILVA, W. A. L. da.; KISCHEL, H.; ARRUDA, E. D. S.; FERREIRA, M. G. C. R.; LIMA, A. C. B. de.; OLIVEIRA, M. V. M.; SOUZA, M. B.; NOGUEIRA, E.; STERZA, F. A. M..
The aim of this study was to evaluate the effect of supplementation with linseed on plasma concentrations of glucose, albumin and cholesterol, as well as the quality of oocytes obtained by ovum pick-up (OPU).
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Cryopreservation; Embryos; Criopreservação; Embrião animal; Embryo (animal); Cryopreservation.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1055133
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Producción in vivo de embriones bovinos F1 (Bos taurus x Bos indicus) durante la época de lluvias y nortes en un agroecosistema tropical. Colegio de Postgraduados
Gordillo Paez, Leonardo.
El objetivo fue determinar el efecto de la temperatura ambiental y la humedad relativa sobre la producción in vivo de embriones bovinos F1, durante la época de lluvias y nortes, en un agroecosistema tropical cálido subhúmedo en Veracruz, México. A 22 vacas Cebú se les indujo ovulación múltiple, y siete días después de la inseminación artificial se recolectaron los embriones. Durante el estudio se monitoreó la humedad relativa y temperatura ambiental, y se asociaron con la producción de embriones. Se utilizó la prueba t de Student para detectar diferencias en la producción de embriones, humedad relativa y temperatura ambiental entre épocas. La producción de embriones y cuerpos lúteos fue mayor (p<0.05) en época de lluvias (1.0 ± 1.1 y 5.1 ± 4.8,...
Palavras-chave: Agroecosistema tropical; Indice temperatura-humedad; Embriones; Tropical agroecosystem; Temperature-humidity index; Embryos; Agroecosistemas tropicales; Maestría.
Ano: 2012 URL: http://hdl.handle.net/10521/1691
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Production of dry type artificial seeds by means of PVC-FOIL desiccation method from genetically engineered somatic embryos of carrot (Daucus carota L., cv. Nantes Duke). Repositório Alice
FARI, M.; SORVARI, S.; AHANEN, K.; MELO, N. F. de; HIRSALMI, H..
bitstream/item/185584/1/Programas-e-resumos-...-ID-21682-p.129.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Semente sintetica; Carrot; Synthetic seeds; Embryos; Biotecnologia; Cenoura; Daucus Carota; Embrião; Biotechnology.
Ano: 1997 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/131984
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Production of wheat starch based artificial endosperm by gel-sandwich technique and plantlet conservion from isolated zygotic embryos in pepper (Capsicum annum Lv. Feherozon). Repositório Alice
FARI, M.; SAZSZ, A.; MITYKÓ, J.; MELO, N. F. de.
Theoretically, the seed-analogue type artificial seed have to be considered as the third generation of the SYNSEED technology. According to this idea, therefore, it should be manufacture this type of artificial seeds with an appropriate artifiical endosperm, which consists of storable, enzymatically digestible and biologically active compounds, to copy the natural seeds. Meanyhile, in the literature of the SYNSEED technology the starch is a neglected compound, however, it is one of the most commonly spread form of storecompound of the endosperm in the seeds of Higher plant. Investigation were carried out in our laboratoy to deveoop appropriate experimental method for the wheat starch, as a potential row material for artificial endosperm. A new method, the...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Semente sintetica; Capsicum annum; Synthetic seeds; Embryos; Embrião; Pimentão; Pepper.
Ano: 1997 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/131983
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