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Diel changes in embryonic maturation in two species of marine cladocerans in Guanabara Bay, Rio de Janeiro, Brazil Zoologia
Marazzo,Andrea; Valentin,Jean L..
Two species of marine cladocerans, Penilia avirostris Dana, 1852 and Evadne tergestina Claus, 1877 were collected in thirteen samples at a fixed station in Guanabara Bay, Rio de Janeiro (30-meter depth) by means of vertical tows with a 200 µm mesh size net at different times from September 12th to 15th, 1995. The parthenogenetic females of Evadne tergestina bore mature embryos (with pigmented eye) only in the samples collected at night, whereas Penilia avirostris at any time of day. This phenomenon was interpreted based on the greater visibility of the Evadne tergestina brood: one of the benefits for embryo maturing during the darkness period would be a decrease of predation on gravid females.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cladocera; Penilia avirostris; Evadne tergestina; Zooplankton; Embryos; Guanabara Bay; Brazil.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000500033
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Distribution of microglial cells in the cerebral hemispheres of embryonic and neonatal chicks BJMBR
Ignácio,A.R.; Müller,Y.M.R.; Carvalho,M.S.L.; Nazari,E.M..
The distribution, morphology and morphometry of microglial cells in the chick cerebral hemispheres from embryonic day 4 (E4) to the first neonatal day (P1) were studied by histochemical labeling with a tomato (Lycopersicon esculentum) lectin. The histochemical analysis revealed lectin-reactive cells in the nervous parenchyma on day E4. Between E4 (5.7 ± 1.35 mm length) and E17 (8.25 ± 1.2 mm length), the lectin-reactive cells were identified as ameboid microglia and observed starting from the subventricular layer, distributed throughout the mantle layer and in the proximity of the blood vessels. After day E13, the lectin-reactive cells exhibited elongated forms with small branched processes, and were considered primitive ramified microglia. Later, between...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microglia; Cerebral hemispheres; Embryos; Neonatal chicks; Gallus domesticus; Tomato lectin.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100008
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Effects of cryoprotectant and plunging temperature in liquid nitrogen on the in vitro and in vivo development of murine morulae Braz. J. Vet. Res. Anim. Sci.
PANTANO,Thaís; MELLO,Marco Roberto Bourg de; GARCIA,José Fernando; HO,Linda Lee; VISINTIN,José Antonio.
The effects of plunging temperature in liquid nitrogen and cryoprotectant dilution methods were evaluated for compacted mouse morulae frozen in 1.5 M ethylene-glycol (E), 1.5M propylene-glycol (P) or 1.4M glycerol (G). Morulae were equilibrated for 10 minutes in cryoprotectant solution and loaded into 0.25 ml straws with cryoprotectant solution in 3 columns (groups E1, P1, G1) or cryoprotectant in the center and PBS in the lateral columns (E2, P2). Straws were cooled at 0.5ºC/min to -25 or -30ºC and plunged into liquid nitrogen. Straws were thawed in water at 22ºC for 20 seconds. Cryoprotectant was diluted in 3 steps for group G1 and in one step for groups E1 and P1 (direct transfer to PBS + 10% FCS) and E2 and P2 (shaken to mix the 3 columns before...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryos; Cryopreservation; Glycerol; Ethylene glycol; Propanediols.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000300012
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Endocrine profiles and ovulation rate of cows superovulated with FSH following passive immunization against steroid free-bovine follicular fluid Braz. J. Vet. Res. Anim. Sci.
ALVAREZ,Rafael Herrera; CARVALHO,João Batista Pereira de; ROSA E SILVA,Alzira; PERONE,Cibele Nunes; RIBELA,Maria Tereza Carvalho Pinto; OLIVEIRA FILHO,Enoch Borges de.
Ten multiparous non-lactating cows were randomly assigned to one of two groups. On days 8 to 12 after estrus, the first group was given 100 ml antiserum against steroid-free bovine follicular fluid (anti-bFF), which was produced in ovariectomized sheep. The second group (control) was given 100 ml nonimmune ovariectomized sheep serum. Six hours after injection, both groups were superovulated with FSH (18 NIH-FSH-S1 units) and LH (0.29 NIH-LH-S1 units) in a 4-day decreasing regime. On the morning of the third day a luteolytic dose of prostaglandin F2alpha analogue (cloprostenol) was given. Artificial inseminations (AI) were done 48 and 60 h later. Embryos were collected 7 days after AI by a nonsurgical method. Blood samples were taken throughout the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Superovulation; Follicular fluid; Passive immunization; Embryos; Cattle.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961998000600007
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Enzymatic polymorphism study as help for constitution of initial broodstock for a new cultivated finfish species. ArchiMer
Renno, J; Luquet, P.
Leporinus friderici is a South American promising species for fish culture. In French Guiana the "morphological species", L. friderici, L. granti, L. lebaili and L. affinis steyermarki are found. Their identification is sometimes difficult on usual criterion. To constitute an initial broodstock of L. friderici from local wild populations, the genetic structure of these species was studied. Fifteen enzyme systems, representing twenty loci, were screened.
Tipo: Text Palavras-chave: French Guiana; Leporinus; Leporinus granti; Leporinus friderici; Anostomidae; Pisces; Embryos; Mortality; Genetics; Freshwater fish; Rearing; Breeding; Biopolymorphism; Brood stocks; Enzymatic activity.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/acte-1441.pdf
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Evaluation of the Relationship Between the Breeder’s Age, Breed and the Incubator Types on Quality and Animal Welfare in the Hatchery Rev. Bras. Ciênc. Avic.
Oro,CS; De Souza,FEZ; Rodrigues,WC; Oro,JS; Guirro,ECBP.
ABSTRACT About 33.3% of chicken life is conditioned by the result of embryonic development. Therefore, understanding and improving the early phase of the broiler’s life is critical to guarantee their well-being. Thus, the objective of this research was to evaluate the relationship between the breeder’s age, breed and the incubator types on quality and animal welfare in the hatchery. The experimental design was completely randomized in a 2 x 2 x 3 factorial arrangement (incubator, breed and age), with 12 treatments and 6 replicates each. The eggs derived of Cobb 500 and Ross’s breeders at the beginning of the laying period, middle age and at the end of the laying period. It is concluded that the size of the eggs is related to the breeder’s age and breed,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryos; Hatch; Poultry.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300304
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Hematological values and body, heart and liver weights of male and female broiler embryos of young and old breeder eggs Rev. Bras. Ciênc. Avic.
Morita,VS; Boleli,IC; Cargnelutti Filho,A.
This study analyzed broiler breeder age (29 or 60 wk-old) effects on physical characteristics of eggs (initial mass, mass loss, volume, diameter, surface area and density) and of eggshells(weight, volume, thickness, conductance, and porosity ), as well as the influence of embryo sex on hematological parameters and body, liver and heart weights during incubation (at days 13, 15, 18 and 21). Physical parameter values were lower in 29-wk-old broiler breeder eggs than those of 60-wk-old breeders, except for relative eggshell weight, which was higher. In both male and female embryos, erythrocytic parameters and the body, liver, and heart weights increased during the incubation. The embryos and their organs were heavier when derived from 60-wk-old breeder eggs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood parameters; Breeder age; Embryos; Eggshell porosity and conductance; Sex.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000100002
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Importancia de los lípidos en la reproducción y ontogenia del pez blanco de Pátzcuaro (Chirostoma estor estor) OceanDocs
Aparicio, B..
Para producir larvas de peces con buenos rendimientos, se deben conocer los requerimientos nutricionales de la especie, particularmente en los primeros estadios larvarios. Además, se sabe que la nutrición de los reproductores influye directamente en la calidad de las larvas debido, principalmente, a la cantidad y calidad de lípidos que son transferidos desde la gónada hacia los huevos. El objetivo de este trabajo fue analizar la composición de lípidos en huevos, embriones y larvas de pez blanco de Pátzcuaro (Chirostoma estor estor) con el fin de establecer las bases de los requerimientos nutricionales de lípidos durante el desarrollo larvario del pez blanco. También se determinaron los ácidos grasos en tejidos de reproductores (cerebro, gónada, hígado,...
Tipo: Theses and Dissertations Palavras-chave: 1079; Lipids; Reproduction; Fish culture; Fish eggs; Embryos; Fish culture; Lipids; Reproduction; Http://aims.fao.org/aos/agrovoc/c_2918; Http://aims.fao.org/aos/agrovoc/c_4362; Http://aims.fao.org/aos/agrovoc/c_6507.
Ano: 2004 URL: http://hdl.handle.net/1834/3465
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Karyotype characterization, constitutive heterochromatin and nucleolus organizer regions of Paranaita opima (Coleoptera, Chrysomelidae, Alticinae) Genet. Mol. Biol.
Almeida,Mara Cristina de; Campaner,Carlos; Cella,Doralice Maria.
Species of the subtribe Oedionychina not only have a highly uniform diploid number of 2n = 22 (20+X+y) but have the karyotypic peculiarity of possessing extremely large sex chromosomes. We analyzed Paranaita opima embryos and gonadal cells to determine their diploid number, chromosomal morphology, type of sex determination system, constitutive heterochromatin pattern and which chromosomes bear nucleolus organizer regions (NORs). The diploid number of P. opima was 2n = 22 (20+XY/XX) with all chromosomes being metacentric. Chromosome pair 6 showed an interstitial secondary constriction on the short arm. The C-banding technique revealed centromeric constitutive heterochromatin in all chromosomes, which, in pair 6, extended up to the secondary constriction of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: C bands; Chromosome; Embryos; Fluorochromes; NOR.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300013
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La densite, un critere de qualite applicable en ecloserie aux embryos de Pecten maximus (mollusque bivalve) ArchiMer
Faure, Catherine; Devauchelle, Nicole; Dorange, Germaine.
The quality of the scallop embryos is determined by the spawners characteristics as well as by the external factors they support during the maturation and the spawning phases. All these parameters are not still well controlled during the artificial conditioning in the hatcheries. As a consequence, the quality of the embryos is drastically variable and with them, the larvae performances in terms of survival and growth rates. It would be interesting to get a simple and quick test which would allow the estimation of the spawns quality. The embryos density, evaluated by isopycnic centrifugation of Percoll medium, is well adapted to this objective. Actually, a centrifugation of embryos two hours after fertilization separates them in 3 stratum with their density...
Tipo: Text Palavras-chave: Pecten maximus; Bivalvia; Quality; Survival; Larvae; Growth; Sexual maturity; Spawning populations; Spawning; Embryos; Hatcheries.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-915.pdf
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Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos Scientia Agricola
Takada,Luciana; Martins Junior,Alicio; Mingoti,Gisele Zoccal; Balieiro,Julio César Carvalho; Coelho,Lia Alencar.
Melatonin (MEL) acts as a powerful scavenger of free radicals and direct gonadal responses to melatonin have been reported in the literature. Few studies, however, have evaluated the effect of MEL during in vitro maturation (IVM) on bovine embryos. This study tested the addition of MEL to maturation medium (MM) with no gonadotropins on nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos. Cumulus-oocyte complexes were aspirated from abattoir ovaries and cultured in MM (TCM-199 medium supplemented with 10% fetal calf serum - FCS) at 39ºC and 5% CO2 in air. After 24 hours of culture in MM with 0.5 µg mL-1 FSH and 5.0 µg mL-1 LH; 10-9 M MEL) or 10-9 M MEL, 0.5 µg mL-1 FSH and 5.0 µg mL-1 LH, the oocytes were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Comet assay; Embryos; Gonadotropins; In vitro fertilization; Melatonin.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000400003
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Nanoplastics impaired oyster free living stages, gametes and embryos ArchiMer
Tallec, Kevin; Huvet, Arnaud; Di Poi, Carole; Gonzalez-fernandez, Carmen; Lambert, Christophe; Petton, Bruno; Le Goic, Nelly; Berchel, Mathieu; Soudant, Philippe; Paul-pont, Ika.
In the marine environment, most bivalve species base their reproduction on external fertilization. Hence, gametes and young stages face many threats, including exposure to plastic wastes which represent more than 80% of the debris in the oceans. Recently, evidence has been produced on the presence of nanoplastics in oceans, thus motivating new studies of their impacts on marine life. Because no information is available about their environmental concentrations, we performed dose-response exposure experiments with polystyrene particles to assess the extent of micro/nanoplastic toxicity. Effects of polystyrene with different sizes and functionalization (plain 2-μm, 500-nm and 50-nm; COOH-50 nm and NH2-50 nm) were assessed on three key reproductive steps...
Tipo: Text Palavras-chave: Oyster; Embryos; Gametes; Microplastics; Nanoplastics.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00453/56419/58090.pdf
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Observaciones sobre embriones de larvas y juveniles de la pescadilla (Cynoscion striatus) OceanDocs
de Ciechomski, J.D.; Cassia, M.C..
Observations on the embrionic and larval development of Cynoscion striatus are presented. The description are made on the basis of material obtained by artificial fertilization. Eggs are pelagic, spherical, 700-840µ in diameter and with single oil globule of 210µ-240µ. The yolk is homogeneous and the chorion has smooth surface. The embryonic development is short and at temperatures of 19°-20°C hatching takes place, 34-36 hours after the fertilization. The larvae at hatching are small, about 2 mm in length and not advanced in their development. The mouth is not functional and the anus is not open. The oil globule is situated in the posterior part of the yolk sac; the position of the anus is close to the posterior end of the yolk sac and the digestice tract...
Tipo: Journal Contribution Palavras-chave: Embryos; Fish larvae; Fish larvae; Http://aims.fao.org/aos/agrovoc/c_2924.
Ano: 1982 URL: http://hdl.handle.net/1834/2045
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Producción in vivo de embriones bovinos F1 (Bos taurus x Bos indicus) durante la época de lluvias y nortes en un agroecosistema tropical. Colegio de Postgraduados
Gordillo Paez, Leonardo.
El objetivo fue determinar el efecto de la temperatura ambiental y la humedad relativa sobre la producción in vivo de embriones bovinos F1, durante la época de lluvias y nortes, en un agroecosistema tropical cálido subhúmedo en Veracruz, México. A 22 vacas Cebú se les indujo ovulación múltiple, y siete días después de la inseminación artificial se recolectaron los embriones. Durante el estudio se monitoreó la humedad relativa y temperatura ambiental, y se asociaron con la producción de embriones. Se utilizó la prueba t de Student para detectar diferencias en la producción de embriones, humedad relativa y temperatura ambiental entre épocas. La producción de embriones y cuerpos lúteos fue mayor (p<0.05) en época de lluvias (1.0 ± 1.1 y 5.1 ± 4.8,...
Palavras-chave: Agroecosistema tropical; Indice temperatura-humedad; Embriones; Tropical agroecosystem; Temperature-humidity index; Embryos; Agroecosistemas tropicales; Maestría.
Ano: 2012 URL: http://hdl.handle.net/10521/1691
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Superovulación en ganado criollo lechero tropical. Colegio de Postgraduados
Rosales Martínez, Froylan.
Se desconoce la respuesta superovulatoria de la hembra Criollo Lechero Tropical (CLT) a la administración de la Hormona Folículo Estimulante (FSH) y se requiere superovular hembras genéticamente superiores. El objetivo del presente estudio fue establecer el mejor protocolo de ovulación múltiple en hembras CLT utilizando FSH. Se realizaron dos ensayos en dos unidades ganaderas de la Región Central del estado de Veracruz de clima cálido subhúmedo. En el primer ensayo se utilizaron 12 vaquillas (35.0±1.7 meses y 404.9±11.6 kg) y en el segundo 6 vaquillas y 5 vacas (50.6±6.2 meses y 429.6±12.1 kg). Las hembras pastorearon pasto estrella (Cynodon plectostachyus), grama nativa (Paspalum ssp) y pasto pará (Brachiaria mutica) con un suplemento comercial con 18% de...
Palavras-chave: Embriones; Bovino criollo; FSH; Veracruz; Embryos; Criollo cattle; Maestría; Ganadería.
Ano: 2013 URL: http://hdl.handle.net/10521/1908
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Superovulatory response, production and quality of embryos of cows fed on linseed or canola seed supplemented diets - doi: 10.4025/actascianimsci.v34i3.13372 Animal Sciences
Albuquerque, Karina Perohouskei; Universidade Estadual de Maringá; Prado, Ivanor Nunes do; Universidade Estadual de Maringá; Prado, Rodolpho Martin do; 9850-7326; Cavallieri, Fábio Luiz Bin; Centro Universitário de Maringá; Rigolon, Luiz Paulo; Universidade Estadual de Maringá; Barbosa, Orlando Rus; Universidade Estadual de Maringá.
Superovulatory response, production and embryo quality and the effects of seasonal changes on embryo production of Nellore cows fed on either linseed (n-3) or canola seeds (n-6) were evaluated. Sixteen Nellore cows (550 ± 48.0 kg) were fed on three diets: control diet (CON), diet with linseed (LIN) and diet with canola seed (CAN) during four seasons. There was no difference in superovulatory response and in total corpora lutea produced when diets are taken into consideration. The number of cows that responded to superovulatory treatment was higher in the winter (93.8%) than in the summer (62.5%). No difference with regard to the three diets existed on the average number of total structures (6.20, 4.96 and 6.50), unfertilized structures (2.53, 1.17 and...
Tipo: Pesquisa Empírica de Campo Palavras-chave: Season of the year; Diets; Embryos; Fatty acids; Fertility; Omega 3; Omega 6.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/13372
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The effect of cigarette smoke on fertilization and pre-implantation development: assessment using animal models, clinical data, and stem cells Biol. Res.
Talbot,Prue; Lin,Sabrina.
Numerous studies have repeatedly shown that women who smoke experience problems establishing and maintaining pregnancies, and recent work has further demonstrated that the in utero effects of smoke may not be manifested until months or even years after birth. The purpose of this review is to examine the recent literature dealing with the effects of cigarette smoke on the earliest stages of human prenatal development. Studies in this area have included the use of animal models, patients undergoing in vitro fertilization, and embryonic stem cell models. Events leading to fertilization, such as cumulus expansion, hyperactivation of sperm motility, and oocyte pick-up by the oviduct are all impaired by smoke exposure in animal models. Steps crucial to...
Tipo: Journal article Palavras-chave: Cigarette smoke; Embryos; Fertilization; IVF; Stem cells; Toxicology.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200011
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The metabolic status of diapause embryos of <i>Artemia franciscana</i> (SFB) OMA
Clegg, J.S.; Drinkwater, L.E.; Sorgeloos, P..
The brine shrimp <i><i>Artemia</i> franciscana</i> is widely used in aquaculture and basic research in areas ranging from molecular biology to evolution and ecology. A key of its life history involves the production of encysted embryos whose development is halted (enters diapause) at the gastrula stage. These shelled embryos are released into the aqueous environment where diapause continues until terminated by suitable conditions that produce an "activated embryo", which then can resume development when conditions permit. Very little is known about the metabolism of diapause embryos, in contrast to activated embryos, which have been studied extensively. We have examined selected features of metabolism in diapause embryos produced in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diapause; Embryos; Metabolism; Artemia franciscana.
Ano: 1996 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2924
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Vitrification of oocytes and in vitro produced bovine embryos exposed to cytochalasin B Braz. J. Vet. Res. Anim. Sci.
Mezzalira,Alceu; Viera,Arnaldo Diniz; Barbieri,Dilmar Paulo; Machado,Mariana Fernandes; Thaler Neto,André; Bernadi,Mari Lourdes; Silva,Carlos Antônio Mondino; Rubin,Mara Iolanda Batistella.
In this study, the influence of cytochalasin B on the survival and development after vitrification of oocytes and in vitro produced (IVP) bovine blastocysts was evaluated. In the Experiment I, 956 oocytes were matured for 22h and were immediately vitrified (Vitri treatment) or exposed for 15-20 minutes, to 7.5µg/ml (CB7.5Vitri treatment) or 45µg/ml (CB45Vitri treatment) cytochalasin B solutions, before vitrification. After 30 seconds of exposure to SV1 [400µl TCM-Hepes with 10% fetal serum (SF), 50µl ethylene glycol (EG) and 50µl DMSO], and 20 seconds to SV2 (300µl trehalose 1,0M + 20% SF, 100µl EG and 100µl DMSO) solutions, the oocytes were vitrified in Open Pulled Straws (OPS). The rewarming was performed at 37-38ºC in two steps of 5 minutes each, into...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Cytochalasin B; Embryos.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000500008
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