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Registros recuperados: 51
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Antimicrobial resistance in Enterobacteriaceae in Brazil: focus on β-lactams and polymyxins BJM
Sampaio,Jorge Luiz Mello; Gales,Ana Cristina.
ABSTRACT During the last 30 years there has been a dissemination of plasmid-mediated β-lactamases in Enterobacteriaceae in Brazil. Extended spectrum β-lactamases (ESBL) are widely disseminated in the hospital setting and are detected in a lower frequency in the community setting. Cefotaximases are the most frequently detected ESBL type and Klebsiella pneumoniae is the predominant species among ESBL producers. Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae became widely disseminated in Brazil during the last decade and KPC production is currently the most frequent resistance mechanism (96.2%) in carbapenem resistant K. pneumoniae. To date KPC-2 is the only variant reported in Brazil. Polymyxin B resistance in KPC-2-producing K. pneumoniae...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ESBL; KPC; NDM; Extended spectrum β-lactamases; New Delhi metallo-β-lactamase; Klebsiella pneumoniae carbapenemase; Polymyxin B; Colistin; Antimicrobial resistance; Carbapenemases; Brazil; Enterobacteriaceae.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000500031
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Cefepime versus extended spectrum β-lactamase-producing Enterobacteriaceae BJID
Nogueira,Keite da Silva; Daur,Alessandra Vale; Reason,Iara Taborda de Messias; Gales,Ana Cristina; Costa,Libera Maria Dalla.
The objective of this study was to evaluate the susceptibility to cefepime of a large group of ESBL- producing enterobacteria recently isolated in a Brazilian teaching hospital . The study included 280 strains of ESBL-producing enterobacteria, isolated between 2005 and 2008. The presence of the genes blaCTX-M, blaTEM and blaSHV was determined by PCR and confirmed by nucleotide sequencing. Susceptibility testing for cefepime was performed by disc-diffusion, agar dilution method and E-test®. Among the isolates, 34 (12.1%) presented a cefepime inhibition zone &gt; 21 and MIC < 8 mg/L by agar dilution and E-strip methods. The use of cefepime for the treatment of infections caused by ESBL-producing bacteria has been controversial. Some studies of PD/PK...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterobacteriaceae; Beta-lactamases; Cephalosporin resistance.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200014
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Ceftolozane-tazobactam activity against drug-resistant Enterobacteriaceae and Pseudomonas aeruginosa causing healthcare-associated infections in Latin America: report from an antimicrobial surveillance program (2013-2015) BJID
Pfaller,Michael A.; Shortridge,Dee; Sader,Helio S.; Gales,Ana; Castanheira,Mariana; Flamm,Robert K..
ABSTRACT This study evaluated the in vitro activity of ceftolozane-tazobactam and comparator agents tested against Latin American isolates of Enterobacteriaceae and Pseudomonas aeruginosa from patients with health care-associated infections. Ceftolozane-tazobactam is an antipseudomonal cephalosporin combined with a well-established β-lactamase inhibitor.A total of 2415 Gram-negative organisms (537 P. aeruginosa and 1878 Enterobacteriaceae) were consecutively collected in 12 medical centers located in four Latin American countries. The organisms were tested for susceptibility by broth microdilution methods as described by the CLSI M07-A10 document and the results interpreted according to EUCAST and CLSI breakpoint criteria. Results: Ceftolozane-tazobactam...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ceftolozane-tazobactam; Drug resistance; Enterobacteriaceae; P. aeruginosa; Latin America; Surveillance.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600627
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Clinical and microbiological observational study on AmpC β-lactamase-producing Enterobacteriaceae in a hospital of Nepal BJID
Baral,Pankaj; Neupanea,Sanjiv; Shresthac,Basudha; Ghimirea,Kashi Ram; Marasinia,Bishnu Prasad; Lekhaka,Binod.
Limited information is available regarding AmpC β-lactamase (ABL)-producing Enterobacteriaceae compared to extended-spectrum β-lactamase-producing enterobacteria. Since ABL-producing organisms are often resistant to multiple antimicrobial agents, therapeutic options against these pathogens are limited. Among 230 clinical Enterobacteriaceae isolates, 64 (27.8%) were found to produce ABL in our study. Escherichia coli (83.9%) was a predominant pathogen, followed by Citrobacter freundii (5.2%). A significant proportion of ABL-producing isolates (81.3%) were found to be multidrug resistant against commonly used antibiotics. Univariate analysis showed that prior history of taking antibiotics (odds ratio [OR], 5.278; confidence interval [CI], 2.838-9.817; p <...
Tipo: Info:eu-repo/semantics/other Palavras-chave: AmpC β-lactamase; Enterobacteriaceae; Antibiotic resistance.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200022
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Communities of P-solubilizing bacteria, fungi and arbuscular mycorrhizal fungi in grass pasture and secondary forest of Paraty, RJ - Brazil Anais da ABC (AABC)
Souchie,Edson L.; Saggin-Júnior,Orivaldo J.; Silva,Eliane M.R.; Campello,Eduardo F.C.; Azcón,Rosario; Barea,Jose M..
Communities of P-solubilizing bacteria, fungi and arbuscular mycorrhizal fungi, were evaluated in two different ecosystems. Samplings taken from two areas of Atlantic forest, in Paraty - RJ, Brazil, one with a secondary forest and the other with a grass pasture were studied. Four growth media: GL (glucose and yeast extract), GES (glucose, soil extract, KNO3, CaCl2, MgSO4, NaCl, FeEDTA and micronutrients solution), GAGES (glucose, soil extract, arabinose, glycerol, CaCl2, MgSO4 and NaCl) and GELP (glucose, soil extract, yeast extract, peptone, CaCl2, MgSO4 and NaCl) were evaluated for the isolation of P-solubilizing microorganisms. The identification of P-solubilizing bacteria was based on 16 S rDNA sequence analysis, while the identification of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphate; Enterobacteriaceae; Atlantic Forest; Glomeromycota; Soil microorganisms.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000100016
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Culture-independent approach for endosymbiotic bacterial systematics in Pentatomidae. Repositório Alice
PRADO, S. de S.; ZUCCHI, T.; PANIZZI, A. R.; CÔNSOLI, F. L..
2011
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Endosymbiotic bacterial systematics; Pentatomidae; Enterobacteriaceae.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/917184
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Detecção e avaliação do perfil de sensibilidade antimicrobiana de enterobactérias isoladas de periquitos cara-suja (Pyrrhura griseipectus) em cativeiro Arq. Bras. Med. Vet. Zootec.
Machado,D.N.; Lopes,E.S.; Albuquerque,Á.H.; Bezerra,W.G.A.; Horn,R.V.; Lima,S.V.G.; Siqueira,R.A.S.; Beleza,A.J.F.; Oliveira,F.R.; Cardoso,W.M.; Teixeira,R.S.C..
ABSTRACT This study was conducted to detect enterobacteria in captive Grey-breasted parakeet from a commercial breeding located in the state of Ceara. 25 individual cloacal swabs were collected and this material was subjected to microbiological processing to obtain the bacterial isolation, identification, and evaluation of the sensitivity profile to antimicrobials. The bacterial isolation in ten samples was determined, belonging to the genera Enterobacter, Hafnia, Serratia, Escherichia, Pantoea and Klebsiella, in which these last three corresponded to the bacteria most isolated, with respective percentages of 28.6%, 21.4%, and 21.4%. As for the sensitivity profile, antibiotics to which the bacteria showed more resistance were trimethoprim-sulfamethoxazole...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Isolamento; Enterobacteriaceae; Psitacídeos; Resistência.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000601732
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Detection of resistance genes and evaluation of water quality at zoo lakes in Brazil Ciência Rural
Faria,Ana Carolina Silva de; Godoy,Isabela de; Sanches,Anderson Aparecido Amorim; Iglesias,Gabriela Accardi; Candido,Stefhano Luiz; Paz,Regina Célia Rodrigues da; Nakazato,Luciano; Dutra,Valéria.
ABSTRACT: The investigation of the presence of antibiotic-resistance genes in aquatic environments is important to identify possible reservoirs of resistant microorganisms that could be a threat to human and animal health. The aims of this study were to analyze the presence of genes conferring resistance to antimicrobials in the aquatic environment and to assess the quality of water in zoo lakes. Results showed a pattern of genes conferring resistance to multiple antibiotics and turbidity, which was expected to be due to the presence of contaminants. The most frequent genes were sul I and sul II (sulfonamides), which were present in all the lakes, followed by genes encoding β-lactamases such as blaPSE I (77.8%) and ampC (66.7%). However, tet(K), tet(M),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multidrug resistance; Zoo pollution; Aquatic environment; Enterobacteriaceae.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500860
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Development of a real-time PCR method coupled with a selective pre-enrichment step for quantification of Morganella morganii and Morganella psychrotolerans in fish products ArchiMer
Podeur, Gaetan; Dalgaard, Paw; Leroi, Francoise; Prevost, Herve; Ernborg, Jette; Martinussen, Jan; Hansen, Lars Hestbjerg; Filet, Marie-france.
Histamine fish poisoning is common and due to toxic concentrations of histamine often produced by Gram-negative bacteria in fin-fish products with a high content of the free amino acid histidine. The genus Morganella includes two species previously reported to cause incidents of histamine fish poisoning. Morganella morganii and Morganella psychrotolerans are both strong producer of histamine. However, little is known about the occurrence and critical stages for fish contamination with these bacteria. To elucidate contamination routes of Morganella, specific real-time quantitative PCR (RTi qPCR) methods for quantification of M. morganii and M. psychrotolerans have been developed. Selective primers amplified a 110 bp region of the vasD gene for M....
Tipo: Text Palavras-chave: Histamine-producing bacteria; Tuna; Selective medium; Enterobacteriaceae; Galactokinase; Type VI secretion system.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00254/36551/35096.pdf
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Diversidad de ß-lactamasas en aislamientos clínicos de enterobacterias ABCL
Casabonne,Cecilia; Pérez,Jorgelina; Balagué,Claudia; Fernández,Luisa.
La rápida emergencia de resistencia a antimicrobianos debida a la presencia de b-lactamasas de espectro extendido (BLEE) tiene un impacto significativo en la salud pública. Las BLEEs son enzimas producidas por bacilos gramnegativos y confieren resistencia a las penicilinas, a todas las cefalosporinas y al aztreonam, pero no a los carbapenemes ni a las cefamicinas y la mayoría son inhibidas por el ácido clavulánico. El objetivo de este trabajo fue evaluar la resistencia a antibióticos b-lactámicos en aislamientos de Klebsiella pneumoniae, Escherichia coli y Proteus mirabilis y caracterizar las b-lactamasas responsables de dicha resistencia. Se analizaron 2.030 aislamientos (362 Klebsiella pneumoniae, 1.250 Escherichia coli y 175 Proteus mirabilis)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: SS-lactamasas de espectro extendido; Enterobacteriaceae; Reacción en cadena de la polimerasa.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572012000300009
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Effects of a dietary added formaldehyde-propionic acid blend on feed enterobacteria counts and on growing pig performance and fecal formaldehyde excretion Ciência Rural
Sbardella,Maicon; Perina,Danilo do Prado; Andrade,Carla de; Longo,Flavio Alves; Miyada,Valdomiro Shigueru.
The purpose of this study was to evaluate the effects of a dietary added formaldehyde-propionic acid blend on feed enterobacteria counts and on growing pig performance and fecal formaldehyde excretion. Forty pigs (34.2±1.8kg BW) were used in a randomized complete block design experiment with four treatments, five replications per treatment and two animals per experimental unit (pen). The treatments were: 0.0, 1.0, 2.0, and 3.0g of blend kg-1 of feed. No effects (P>0.05) of dietary added blend levels were observed on average daily gain, average daily feed intake and feed:gain ratio during 1 to 14 and 1 to 28 days of experiment. Increasing dietary levels of blend reduced quadratically (P<0.01) total Enterobacteriaceae counts on 1st and 14th day after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Feed additive; Enterobacteriaceae; Swine.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000300474
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Efficacy of polymyxins in the treatment of carbapenem-resistant Enterobacteriaceae infections: a systematic review and meta-analysis BJID
Ni,Wentao; Cai,Xuejiu; Wei,Chuanqi; Di,Xiuzhen; Cui,Junchang; Wang,Rui; Liu,Youning.
In recent years, carbapenem-resistant Enterobacteriaceae has become endemic in many countries. Because of limited treatment options, the abandoned "old antibiotics", polymyxins, have been reintroduced to the clinic. To evaluate the clinical efficacy of polymyxins in the treatment of infections caused by carbapenem-resistant Enterobacteriaceae , we systemically searched the PubMed, Embase, and Cochrane Library databases and analyzed the available evidence. The Preferred Reporting Items for Systematic reviews and Meta-Analysis statement were followed, and the I 2 method was used for heterogeneity. Nineteen controlled and six single-arm cohort studies comprising 1086 patients met the inclusion criteria. For controlled studies, no significant difference was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polymyxin; Enterobacteriaceae; Carbapenem-resistant; Carbapenemase-producing.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000200170
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Elaboration and evaluation of a new screening medium for detection and presumptive identification of extended-spectrum beta-lactamase-producing organisms (ESBL) BJM
Silva,Carlos Henrique Pessôa de Menezes e.
The new ß-lactamases have arisen largely as a consequence of heavy use of new expanded spectrum ß-lactam antibiotics. Health professionals need to be aware of the resistance problems caused by the new enzymes, and know the correct procedures to detect, prevent and control such problems. In this study, a new screening medium called Ceftazidime-Inositol-Vancomycin-Amphotericin B Agar (CIVA) was elaborated and the microbiological performance was evaluated for the detection and presumptive identification of ESBL-producing members of Enterobacteriaceae. It was performed in 126 stool samples from hospitalized patients at Santa Monica Hospital (Vila Velha, ES, Brazil), who had been heavily exposed to broad-spectrum antibiotic combinations. The bacteria were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterobacteriaceae; Culture medium; ESBL.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400006
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Emergence of colistin resistance in the largest university hospital complex of São Paulo, Brazil, over five years BJID
Rossi,Flávia; Girardello,Raquel; Cury,Ana Paula; Di Gioia,Thais Sabato Romano; Almeida Jr,João Nóbrega de; Duarte,Alberto José da Silva.
Abstract Colistin resistance involving Gram-negative bacilli infections is a challenge for health institutions around of the world. Carbapenem-resistance among these isolates makes colistin the last therapeutic option for this treatment. Colistin resistance among Enterobacteriaceae, Acinetobacter spp., and Pseudomonas spp. was evaluated between 2010 and 2014 years, at Hospital das Clínicas, São Paulo, Brazil. Over five years 1346 (4.0%) colistin resistant Gram-negative bacilli were evaluated. Enterobacteriaceae was the most frequent (86.1%) pathogen isolated, followed by Acinetobacter spp. (7.6%), and Pseudomonas spp. (6.3%). By temporal analysis there was a trend for an increase of colistin resistance among Enterobacteriaceae, but not among...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Colistin-resistant; Polymyxin B-resistant; Enterobacteriaceae; Brazil.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100098
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Emergence of Klebsiella pneumoniae-producing KPC-2 carbapenemase in Paraíba, Northeastern Brazil BJID
Fehlberg,Lorena C.C.; Carvalho,Albalucia M.C.; Campana,Eloiza H.; Gontijo-Filho,Paulo P.; Gales,Ana C..
The emergence of KPC-2 producing K. pneumoniae in hospitalized patients at the intensive care unit (ICU) of a teaching hospital located in the city of João Pessoa, Paraíba, Brazil, is reported. Seven carbapenem-resistant K. pneumoniae recovered from different body sites of infection were analyzed. Most isolates showed a multidrug-resistance phenotype. Genotypic analysis demonstrated the presence of two genotypes, with the predominance of genotype A, which belongs to ST 437. These isolates also carry the encoding genes of five other betalactamases.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Beta-lactam resistance; Modified Hodge test; Carbapenem resistance; Enterobacteriaceae.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600013
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Enterobacteriaceae in mouth and cloaca of podocnemis expansa and P. Unifilis (testudines: chelonia) populations of national park of araguaia plains, Brazil BJM
Morais,Paula Benevides de; Souza,Denise Rodrigues de; Sousa,Francisca Maria Pinheiro de; Oliveira,Kleverson Wessel de; Pimenta,Raphael Sanzio.
Shigella flexnerii and Escherichia coli were the most frequent Gram-negative bacteria found in the mouth cavity and cloacae of the turtles Podocnemis expansa and P. unifilis on beaches in the National Park of Araguaia, Brazil. Reptiles are known as Salmonella carriers, despite rarely isolated in these turtles.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Shigella; Enterobacteriaceae; Turtles; Bananal Island.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200017
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Enterobacteriaceae resistant to third generation cephalosporins upon hospital admission: risk factors and clinical outcomes BJID
Oliveira,Mirian Cristina; Oliveira,Clara Rodrigues Alves; Gonçalves,Karine Valéria; Santos,Marciléa Silva; Tardelli,Amanda Cristina Silva; Nobre Jr.,Vandack Alencar.
Objectives: Evaluate risk factors and clinical outcomes of infections caused by Enterobacteriaceae resistant to third-generation cephalosporins present in samples collected upon hospital admission.Methods: Risk factors were evaluated using a 1:2 ratio case-control study. Influence of resistance on the appropriateness of antibiotic therapy, length of stay, and hospital mortality were prospectively evaluated. Characteristics independently associated with the presence of resistant enterobacteria were assessed by logistic regression.Results: Enterobacteria resistant to third-generation cephalosporins were quite common (26.0%). Male gender (OR: 2.66; 95% CI, 1.17-5.06; p= 0.019), invasive prosthesis (OR: 3.79; 95% CI, 1.29-11.08; p= 0.015), previous use of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterobacteriaceae; Third generation cephalosporins; Risk factors; Antibiotic resistance.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000300239
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Environmental parameters and antimicrobial susceptibility of enterobacteriaceae isolated from estuarine waters of São Vicente, São Paulo state, Brazil J. Venom. Anim. Toxins incl. Trop. Dis.
Lourenço,N. G. G. S.; Takahashi,C. K.; Lopes,T. F.; Lopes,C. A. M..
Diseases transmitted by water consists a serious public health problem and enterobacteria are the main group of microorganisms responsible for outbreaks in humans. Such pathogenic bacteria proliferate in water polluted by domestic and industrial sewage and reach the population through seawater contact. The aim of the present work was to study environmental parameters as well as to identify Enterobacteriaceae species and their antimicrobial susceptibility in water samples collected from the estuarine area of São Vicente city (São Paulo State, Brazil). Strains were identified by using traditional biochemical tests described in literature and antimicrobial susceptibility tests were carried out using the disk diffusion method. Out of 26 samples, Escherichia...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Estuarine water; Environmental parameters; Enterobacteriaceae; Antimicrobial susceptibility.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200006
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Evaluation of phenotypic tests to detect carbapenem-resistant Enterobacteriaceae in colonized patients hospitalized in intensive care units BJID
Perez,Leandro Reus Rodrigues; Rodrigues,Diógenes; Dias,Cícero Gomes.
In this study, we aimed to evaluate the performance of different phenotypic tests to detect carbapenem-resistant Enterobacteriaceae. Three different phenotypic methods were evaluated: (1) combined-disk test of meropenem plus phenylboronic acid or EDTA reading after 24 h and 48 h; (2) selective/chromogenic read after 24 h and after 48 h; and (3) overnight selective enrichment broth containing 10 µg ertapenem disk followed by culture on MacConkey agar. A positive result in at least one of the methods was submitted to PCR for blaNDM-1, blaOXA-48, blaKPC, blaSPM-1, blaIMP, and blaGES detection. Carbapenem-resistant Enterobacteriaceae was detected in 31 (30.4%) of 102 rectal swabs evaluated. All isolates showed to be KPC-2-producing organisms. Results showed...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Carbapenems; Enterobacteriaceae; KPC; Surveillance.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000400436
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Evaluation of the PetrifilmTM EB and TEMPO® EB systems with ISO 21528-2:2004 method for the count of Enterobacteriaceae in milk BJM
Cirolini,Andréia; Miotto,Marília; Machado,Fernanda Morgana; Silva,Helen Silvestre da; Ogliari,Paulo José; Vieira,Cleide Rosana Werneck.
The development of alternative microbiological techniques is driven by the necessity to meet the current needs to deliver rapid results in the manufacturing process of foods, but it is important that these methods be evaluated for each application. The objective of the present study was to assess the PetrifilmTM EB and the TEMPO® EB systems with ISO 21528-2:2004 for the count of Enterobacteriaceae in pasteurized and UHT milk samples. We analyzed the microflora of 141 pasteurized milk samples, 15 samples of artificially contaminated pasteurized milk and 15 samples of artificially contaminated UHT milk. Investigation of the method PetrifilmTM EB and ISO 21528:2 regression analysis showed a high correlation in the samples, r = 0.90 for the microflora of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterobacteriaceae; Milk; Alternative methods; ISO 21528-2:2004; Count.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300016
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