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| Registros recuperados: 30 | |
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| Franco,Henrique Coutinho Junqueira; Pimenta,Maria Teresa Borges; Carvalho,João Luís Nunes; Magalhães,Paulo Sérgio Graziano; Rossell,Carlos Eduardo Vaz; Braunbeck,Oscar Antonio; Vitti,André Cesar; Kölln,Oriel Tiago; Rossi Neto,João. |
| Due to new possibilities for using sugarcane (Saccharum spp.) trash for electricity generation, and the production of 2nd generation ethanol and others chemicals, the interest for its recovery has increased. However, the question of how much trash can be removed from sugarcane field still needs to be clarified. This study evaluated the amount of dry matter, nutrients content, structural compounds and efficiency of the enzymatic hydrolysis of the hydrothermal pretreated materials for tops and dry leaves in samples from sugarcane varieties. Tops and dry leaves present differences in nutrients content and moisture. Therefore, the amount of trash to be collected should not be simply based on percentages, but also should take into account the different... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Saccharum spp.; Enzymatic hydrolysis; Sugarcane crop residues. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000500004 |
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| SOUZA, A. M.; VIEIRA, R. F.; RAMOS, N. P.; GONCALVES, J. R. P.. |
| Resumo: O objetivo deste trabalho foi avaliar a atividade microbiológica e enzimática de solos cultivados com cana de açúcar. Os solos, Latossolo Roxo, foram coletados em área de 1 ano de corte A(1), três anos de corte D(3), cinco anos de corte C(5) e 8 anos de corte H(8), nas profundidades de 0-10 cm e 10-20 cm. O solo A(1), ao contrário dos outros, recebe regulares aplicações de vinhaça. Os parâmetros avaliados foram: atividade de hidrólise do diacetato de fluoresceína (HDF), potencial de mineralização de N (PMN) e atividades das enzimas glucosidase e urease. O solo C(5) apresentou os melhores resultados em termos de atividade por unidade de C orgânico do solo. Ressalta-se, porém, que as variabilidades dos dados obtidos foram altas, evidenciando a... |
| Tipo: Artigo em anais de congresso (ALICE) |
Palavras-chave: Microbiologia do solo; Cana de açúcar; Latossolo roxo; Soil biology; Microbiological quality; Enzymatic hydrolysis; Sugarcane. |
| Ano: 2012 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/944535 |
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| SOUZA, R. S. C. de; TONON, R. V.; STEPHAN, M. P.; SILVA, C. M.; PENTEADO, A. L.; CABRAL, L. M. C.; KUROZAWA, L. E.. |
| Resumo: O objetivo do trabalho foi avaliar as atividades antioxidante e antimicrobiana de hidrolisados proteicos de soro de leite obtidos por diferentes proteases (Alcalase e Flavourzyme) e sua mistura (proporção 50:50). Previamente à hidrólise enzimática, o soro de leite foi concentrado por ultrafiltração com membranas cerâmicas tubulares. A reação enzimática foi realizada de acordo com o método pH-stat, sendo coletadas alíquotas com diferentes graus de hidrólise ( GH), ao longo de cada hidrólise proteica. Os hidrolisados foram analisados quanto a estimativa do teor de peptídeos, atividade antioxidante pelos métodos de captura do radical livre ABTS+ e do radical peroxila (Oxygen radical antioxidant capacity - ORAC), eletroforese e atividade... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Hidrólise enzimática; Grau de hidrólise; Capacidade antioxidante; Degree of hydrolysis; Soro de Leite; Proteína; Antioxidante; Proteína Hidrolisada; Peptídeo; Eletroforese; Peptides; Whey; Enzymatic hydrolysis; Antioxidant activity; Electrophoresis. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115788 |
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| Valeriano,Ixthá Hasselmann; Marques,Gabriel Lessa Lisboa; Freitas,Suely Pereira; Couri,Sonia; Penha,Edmar das Mercês; Gonçalves,Márcia Monteiro Machado. |
| ABSTRACT The aim of this study was to produce bioethanol from enzymatic hydrolysates of cassava pulp, a by-product of cassava flour manufacturing, using an alcohol-tolerant Saccharomyces cerevisiae strain. First, the best operational condition of the starch hydrolysis process was determined through a complete factorial design (24), with triplicates at the central point. The independent variables evaluated were: the concentrations of α-amylase (Termamyl 2X) and glucoamylase (AMG 300L) and both liquefaction and saccharification times. The most favorable hydrolysis condition in the assay was achieved using 0.517 mL AMG.g starch-1 and 0.270 mL Termamyl.g starch-1, with liquefaction and saccharification times of 1 and 2 h, respectively. The broth obtained at... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Starch waste; Enzymatic hydrolysis; Alcoholic fermentation. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100207 |
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| HU,Pan; FAN,Xiaoxu; LIN,Lingshang; WANG,Juan; ZHANG,Long; WEI,Cunxu. |
| Abstract Rice starches with different amylose contents were treated with sodium dodecyl sulfate (SDS) to deplete surface proteins and lipids, and the changes in molecular structure, thermal properties, and enzymatic hydrolysis were evaluated. SDS treatment did not significantly change the molecular weight distribution, crystalline structure, short-range ordered degree, and gelatinization properties of starch, but significantly altered the pasting properties and increased the swelling power of starch. The removal of surface proteins and lipids increased the enzymatic hydrolysis and in vitro digestion of starch. The influences of removing surface proteins and lipids from starch on swelling power, pasting properties, and enzymatic hydrolysis were different... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzymatic hydrolysis; Molecular structure; Rice starch; Surface proteins and lipids; Thermal properties. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018000100084 |
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| COLOMBI, B. L.; HEINZ, K. G. H.; ZANONI, P. R. S.; MAGALHAES, W. L. E.; TAVARES, L. B. B.. |
| Ethanol from lignocellulosic biomass (LCB), such as agricultural and forest residues, is known as the second generation (2G) ethanol. Carbohydrate fractions in LCB (cellulose and hemicellulose) are not readily available for microbial fermentation and they need to be released by additional pre-treatment and hydrolysis operations. This process is intrinsically more difficult and costly. In this study the efficiency of enzymatic hydrolysis of the species Eucalyptus urophylla and the hybrid E. urophylla x E. grandis, submitted or not to alkaline pre-treatment with green liquor, has been evaluated. The hydrolysis was carried out with a enzymatic cocktail containing aggressive cellulases, high level of ?-glucosidases and hemicellulase at 6% (g enzyme/g solid),... |
| Tipo: Artigo em anais de congresso (ALICE) |
Palavras-chave: Eucalyptus; Eucalipto; Espécie exótica; Second generation ethanol; Pre-treatment; Enzymatic hydrolysis; Etanol de segunda geração; Madeira; Pré tratamento; Hidrólise enzimática. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1029093 |
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| Yan,Shoubao; Yao,Jianming; Yao,Liming; Zhi,Zhijun; Chen,Xiangsong; Wu,Jingyong. |
| The enzymatic hydrolysis of food waste by commercially available enzymes and the subsequent ethanol fermentation of the hydrolysates by Saccharomyces cerecisiae H058 were studied in this work. The optimum batch enzymatic conditions were found to be saccharification pH of 4.5, temperature of 55!, glucoamylase concentration of 120 u/g, α-amylase concentration of 10 u/g, solid-liquid ratio of 1: 0.75 (w/w). Fed batch hydrolysis process was started with a solid-liquid ratio of 1: 1 (w/w), with solid food waste added at time lapse of 2 h to get a final solid-liquid ratio of 1: 0.5 (w/w). After 4 h of reaction, the reducing sugar concentration reached 194.43 g/L with a enzymatic digestibility of 93.12%. Further fermentation of the batch and fed batch enzymatic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Food waste; Enzymatic hydrolysis; Hydrolysates; Fermentation; Ethanol production. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200002 |
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| Dias,Disney Ribeiro; Abreu,Celeste Maria Patto de; Silvestre,Marialice Pinto Coelho; Schwan,Rosane Freitas. |
| The common bean (Phaseolus vulgaris L.) is a staple food in the Brazilian diet and represents the major source of dietary protein and other micronutrients and minerals. Despite the considerable protein concentration in beans, the food is considered of low biological value when compared to animal proteins and other plant protein sources. To improve the availability of protein in beans, enzymatic treatments were performed in four cultivars (ON, OPNS, TAL and VC3). The approach was a completely randomized design with four replicates. We used a 4 × 3 factorial arrangement (four cultivars and three treatments: treatment 1-addition of commercial protease (Trypsin 250, Difco), treatment 2-addition of protease from Bacillus sp., and treatment 3:-control without... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bean flour; Enzymatic hydrolysis; Alkaline protease. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000100014 |
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| Soufi-kechaou, Emna; Jaouen, Pascal; Ben Amar, Raja; Berge, Jean-pascal. |
| Enzymatic hydrolysis of cuttlefish viscera by Protamex, Pepsin, Delvolase and Multifect Neutral was investigated in a batch reactor during 6 hours with an hourly sampling. The influence of the enzyme used and the hydrolysis conditions (operating pH, temperature) was studied with regards to the extent of proteolytic degradation and to molecular weight distribution of the peptides. After 360 min of hydrolysis, cuttlefish viscera treated with Pepsin yielded significantly (p < 0.05) higher protein recoveries compared to the substrate autolysed or treated with other external proteases. However, the amino acid composition showed that this was Protamex hydrolysis which gave the highest percentage of essential amino acids. At least, according to the FPLC... |
| Tipo: Text |
Palavras-chave: Enzymatic hydrolysis; Cuttlefish Protein Hydrolysates (CPH); Cuttlefish By-Products (CBP); Essential Amino Acids (EAA); Protein recovery. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00114/22508/20191.pdf |
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| BEZERRA, C. A.; MACEDO, L. L. P.; AMORIM, T. M. L.; SANTOS, V. O.; FRAGOSO, R. da R.; LUCENA, W. A.; MENEGUIM, A. M.; VALENCIA-JIMENEZ, A.; ENGLER, G.; SILVA, M. C. M. da; SA, M. F. G. de; FREIRE, E. V. S. A.. |
| Abstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Expressão gênica; Curculionideo; Praga de planta; Amido; Hidrolise; Curculionidae; Gene expression; Insect pests; Starch; Enzymatic hydrolysis. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/1003023 |
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| BEZERRA, C. A.; MACEDO, L. L. P.; AMORIM, T. M. L.; SANTOS, V. O.; FRAGOSO, R. da R.; LUCENA, W. A.; MENEGUIM, A. M.; VALENCIA-JIMENEZ, A.; ENGLER, G.; SILVA, M. C. M.; ALBUQUERQUE, E. V. S.; GROSSI-DE-SA, M. F.. |
| Abstract: a-Amylases are common enzymes responsible for hydrolyzing starch. Insect-pests, whose larvae develop in seeds, rely obligatorily on a-amylase activity to digest starch, as their major food source. Considering the relevance of insect a-amylases and the natural a-amylase inhibitors present in seeds to protect from insect damage, we report here the molecular cloning and nucleotide sequence of the full-length AmyHha cDNA of the coffee berry borer, Hypothenemus hampei, a major insect-pest of coffee crops. The AmyHha sequence has 1879 bp, containing a 1458 bp open reading frame, which encodes a predicted protein with 485 amino acid residues, with a predicted molecular mass of 51.2 kDa. The deduced protein showed 55?79% identity to other insect... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Expressão gênica; Curculionideo; Praga de planta; Amido; Hidrolise; Curculionidae; Gene expression; Insect pests; Starch; Enzymatic hydrolysis. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1006141 |
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| Registros recuperados: 30 | |
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