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Registros recuperados: 38
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A biodegradation study of forest biomass by Aspergillus niger F7: correlation between enzymatic activity, hydrolytic percentage and biodegradation index BJM
Sharma,Nivedita; Kaushal,Richa; Gupta,Rakesh; Kumar,Sanjeev.
Aspergillus niger F7 isolated from soil was found to be the potent producer of cellulase and xylanase. The residue of forest species Toona ciliata, Celtris australis, Cedrus deodara and Pinus roxburghii was selected as substrate for biodegradation study due to its easy availability and wide use in industry. It was subjected to alkali (sodium hydroxide) treatment for enhancing its degradation. Biodegradation of forest waste by hydrolytic enzymes (cellulase and xylanase) secreted by A. niger under solid state fermentation (SSF) was explored. SSF of pretreated forest biomass was found to be superior over untreated forest biomass. Highest extracellular enzyme activity of 2201±23.91 U/g by A. niger was shown in pretreated C. australis wood resulting in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger; SSF; Enzyme activity; Percent hydrolysis; Biodegradation index.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200006
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Aluminum stress tolerance in potato genotypes grown with silicon Bragantia
Dorneles,Athos Odin Severo; Pereira,Aline Soares; Sasso,Victória Martini; Possebom,Gessieli; Tarouco,Camila Peligrinotti; Schorr,Márcio Renan Weber; Rossato,Liana; Ferreira,Paulo Ademar Avela; Tabaldi,Luciane Almeri.
ABSTRACT Potato cultivation is widespread around the world, being exposed to several abiotic stresses, including soils with high aluminum (Al) availability. Silicon (Si) is recognized for alleviating the stress caused by Al in various plant species. Thus, the aim of this study was to investigate the potential of Si to mitigate the oxidative stress caused by Al in potato genotypes, exhibiting differential sensitivity toward this element. Plants of the Al-sensitive genotype (SMIJ319-7) and Al-tolerant genotype (SMIF212-3) were grown for two weeks in a hydroponic system with the nutrient solution containing combinations of Al (0 and 1.85 mM) and Si (0, 0.5 and 1.0 mM). At the end of the experiment, photosynthetic parameters, pigment content, root and shoot...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum tuberosum; Beneficial elements; Enzyme activity; Oxidative stress; Toxic elements.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000100012
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Analysis of methodologies for the study of composition and biochemical carbohydrate changes in harvest and postharvest onion bulbs Phyton
Abrameto1,MA; Pozzo Ardizzi,CM; Gil,MI; Molina,LM.
Costly investments in storage and transport of onion bulbs makes it increasingly important to identify cultivars with the best chance of long-term storage. This paper discusses our own and other researchers' results and laboratory procedures on onion's pungency, soluble solids, dry matter, respiration rate, carbohydrates and catabolism-related enzymes under different storage conditions. The variability in parameters such as carbohydrate composition, pungency, soluble solids and soluble uronic acid concentration in water is growth dependent, and defines the quality of onion bulbs at harvest time. During storage, these parameters can be modified by environmental conditions, presence of pathogens or termination of dormancy. Under postharvest conditions,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allium cepa L.; Respiration rate; Sprouting; Ethylene; Non-structural carbohydrates; Enzyme activity.
Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000200003
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Atributos biológicos em área de integração lavoura-pecuária. Infoteca-e
SCHLEMMER, F.; QUIXABEIRA, J.; NEVES, S. S.; MENDES, I. C.; REIS JÚNIOR, F. B..
bitstream/item/78928/1/p2007-10.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) Palavras-chave: Biomassa microbiana; Crop-livestock; Soil microbiology.; Atividade Enzimática; Carbono; Microbiologia do Solo.; Enzyme activity; Carbon; Microbial biomass..
Ano: 2007 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/569879
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Biochemical and physiological analysis in carrot seeds from different orders of umbels Rev. Ciênc. Agron.
Silva,Patrícia Pereira da; Sekita,Marcelo Coelho; Dias,Denise Cunha Fernandes dos Santos; Nascimento,Warley Marcos.
ABSTRACT In carrot seed production, harvesting of the umbels is generally staggered, or sometimes the whole plant is harvested with the umbels at different stages of maturation; in that case, there is a risk of harvesting immature seeds and of losses due to natural dehiscent, or to physiological deterioration as a result of unfavourable environmental factors. The present study aimed to evaluate the physiological and biochemical changes related to the maturation of 'Planalto' carrot seeds from diferent umbels. The samples were collected 120 days after anthesis, with the primary, secondary and tertiary umbels being harvested separately. Seeds were analysed for germination, first count and germination rate, at temperatures of 25 ºC (optimum) to 35 ºC...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Daucus carota L.; Physiological quality; Enzyme activity.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902016000200407
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Biochemical and toxinological characterization of Naja sumatrana (Equatorial spitting cobra) venom J. Venom. Anim. Toxins incl. Trop. Dis.
Yap,MKK; Tan,NH; Fung,SY.
The lethal and enzymatic activities of venom from Naja sumatrana (Equatorial spitting cobra) were determined and compared to venoms from three other Southeast Asian cobras (Naja sputatrix, Naja siamensis and Naja kaouthia). All four venoms exhibited the common characteristic enzymatic activities of Asiatic cobra venoms: low protease, phosphodiesterase, alkaline phosphomonoesterase and L-amino acid oxidase activities, moderately high acetylcholinesterase and hyaluronidase activities and high phospholipase A2. Fractionation of N. sumatrana venom by Resource® S cation exchange chromatography (GE Healthcare, USA) yielded nine major protein peaks, with all except the acidic protein peak being lethal to mice. Most of the protein peaks exhibit enzymatic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Naja; Cobra venom; Enzyme activity; Cardiotoxin.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400012
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Bioprospecting of lipolytic microorganisms obtained from industrial effluents Anais da ABC (AABC)
PEIL,GREICE H.S.; KUSS,ANELISE V.; RAVE,ANDRÉS F.G.; VILLARREAL,JOSÉ P.V.; HERNANDES,YOHANA M.L.; NASCENTE,PATRÍCIA S..
ABSTRACT The lipases have ability to catalyze diverse reactions and are important in different biotechnological applications. The aim of this work was to isolate and characterize microorganisms that produce lipases, from different food industry effluents localized in Pelotas, RS/Brazil. Bacteria were identified using Gram stain and biochemical tests (Vitek 2(r)). Fungi were identified according to macro and micromorphology characteristics. The extracellular lipase production was evaluated using the Rhodamine B test and the enzymatic activity by titration. Twenty-one bacteria were isolated and identified as Klebsiella pneumoniae ssp. pneumoniae, Serratia marcescens, Enterobacter aerogenes, Raoultella ornithinolytica and Raoultella planticola. Were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Effluent; Enzyme activity; Extracellular lipase production; Lipase enzyme; Lipolytic microorganism.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501769
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Bordonein-L, a new L-amino acid oxidase from Crotalus durissus terrificus snake venom: isolation, preliminary characterization and enzyme stability J. Venom. Anim. Toxins incl. Trop. Dis.
Bordon,Karla C. F.; Wiezel,Gisele A.; Cabral,Hamilton; Arantes,Eliane C..
BackgroundCrotalus durissus terrificus venom (CdtV) is one of the most studied snake venoms in Brazil. Despite presenting several well known proteins, its L-amino acid oxidase (LAAO) has not been studied previously. This study aimed to isolate, characterize and evaluate the enzyme stability of bordonein-L, an LAAO from CdtV.Methods The enzyme was isolated through cation exchange, gel filtration and affinity chromatography, followed by a reversed-phase fast protein liquid chromatography to confirm its purity. Subsequently, its N-terminal amino acid sequence was determined by Edman degradation. The enzyme activity and stability were evaluated by a microplate colorimetric assay and the molecular mass was estimated by SDS-PAGE using periodic acid-Schiff...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crotalus durissus terrificus; L-amino acid oxidase; Rattlesnake; Enzyme activity; Enzyme stability; Chromatography; Snake venom; Yellow venom; Stabilization.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100335
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Caracterización del sistema enzimatico de los hongos comestibles (Pleurotus) como una estrategia de selección de cepas para la producción rural Colegio de Postgraduados
Lima Peralta, Rosa María de.
En el presente trabajo se investigo la producción extracelular de celulasas en medio líquido con 12 cepas nativas mexicanas y una comercial de Pleurotus. También se determino el contenido de proteína en los extractos y el peso seco de las colonias. Y se determino el coeficiente de correlación entre las variables producción de celulasa y proteína. Se encontró que el mejor medio de cultivo líquido para la producción de celulasas fue el sugerido por Elisashvili et al (2008a), con algunas modificaciones. Todas las cepas de Pleurotus crecieron sobre el medio de cultivo líquido con celulosa (Solka Floc) como fuente de carbono debido a que pudo producir enzimas celulolíticas para degradar el substrato. Los resultados muestran que la actividad enzimática se...
Tipo: Tesis Palavras-chave: Especies de Pleurotus; Fermentación líquida; Actividad enzimática; Producción de proteína; Producción de biomasa; Maestría; EDAR; Estrategias para el Desarrollo Agrícola Regional; Pleurotus species; Liquid fermentation; Enzyme activity; Protein production; Biomass production.
Ano: 2009 URL: http://hdl.handle.net/10521/1556
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Characteristics of glucose oxidase immobilized on Magnetic Chitosan Nanoparticles Ciênc. Tecnol. Aliment.
EIN ALI AFJEH,Maryam; POURAHMAD,Rezvan; AKBARI-ADERGANI,Behrouz; AZIN,Mehrdad.
Abstract Immobilization process is used to facilitate the enzyme recovery and reusability. The aims of this study were to evaluate the effect of different amounts of chitosan on immobilization efficiency of Glucose Oxidase (GOX), pH effect on enzyme activity and changes of kinetic characteristics of enzyme immobilized on Magnetic Chitosan Nanoparticles (MCNP). GOX was immobilized on MCNP with cross bonds and Schiff base covalent connections. The sample with 0.3 g of chitosan had the highest immobilization efficiency (41.30%). Morphology of nanoparticles was investigated by Scanning Electron Microscopy (SEM). Immobilization of GOX on magnetic nanoparticles was verified with Fourier Transform Infrared (FTIR). The effect of pH on enzyme activity was similar...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitosan nanoparticles; Enzyme activity; Glucose oxidase; Immobilization efficiency; Kinetic parameters.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000100068
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Characterization of polyphenol oxidase in two cocoa (Theobroma cacao L.) cultivars produced in the south of Bahia, Brazil Ciênc. Tecnol. Aliment.
MACEDO,Adrielle Souza Leão; ROCHA,Fátima de Souza; RIBEIRO,Margareth da Silva; SOARES,Sergio Eduardo; BISPO,Eliete da Silva.
Abstract The reactions leading to the formation of precursors of chocolate flavor are performed by endogenous enzymes present in the cocoa seed. Polyphenol oxidase (PPO) presence and activity during fermentation of cocoa beans is responsible for the development of flavor precursors and is also implicated in the reduction of bitterness and astringency. However, the reliability of cocoa enzyme activities is complicated due to variations in different genotypes, geographical origins and methods of fermentation. In addition, there is still a lack of systematic studies comparing different cocoa cultivars. So, the present study was designed to characterize the activity of PPO in the pulp and seeds of two cocoa cultivars, PH 16 and TSH 1188. The PPO activity was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cocoa; Fermentation; Enzyme activity; Chocolate.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000100056
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Concentrated beta-galactosidase and cell permeabilization from Saccharomyces fragilis IZ 275 for beta-galactosidase activity in the hydrolysis of lactose Ciênc. Tecnol. Aliment.
MORIOKA,Luiz Rodrigo Ito; VIANA,Caroline dos Santos; ALVES,Érika de Pádua; PAIÃO,Fernanda Gonzales; TAKIHARA,Anderson Massaro; KAKUNO,Amanda Sayuri Senoo; SUGUIMOTO,Hélio Hiroshi.
Abstract The cheese whey shows an organic nutrient charge that can be used to obtain metabolites of interest by biotechnology of microorganisms. Thus, fermentative processes for enzyme production, in particular beta-galactosidase becomes feasible. The enzyme plays an important role in the biotech food industry to obtain milk and dairy products with low lactose content for consumption by intolerant individuals. The objective of this work was to determine the enzyme activity of the concentrated beta-galactosidase (CBG) and the permeabilized cells (PC) both obtained from Saccharomyces fragilis IZ 275. The enzyme beta-galactosidase obtained from the fermentation of Saccharomyces fragilis IZ 275 in cheese whey was used to determine the optimal conditions for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme activity; Microbial biotechnology; Cell permeabilization; Lactose hydrolysis; Enzyme extraction.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000300524
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Dietary levels and sources of selenium for post weaning piglets Ciência Rural
Oliveira,Tiago Ferreira Birro; Bertechini,Antônio Gilberto; Philomeno,Renato; Silva,Vanessa Avelar.
ABSTRACT: An experiment was conducted to study the effects of two sources and dietary levels of selenium (Se) on performance, Se concentration and glutathione peroxidase activity in plasma and liver of piglets post weaning. A total of 126 weaned pigs male and female Large White × Landrace, at average body weight of 7.2±0.67kg were allotted to seven treatments in a randomized block design, with two Se sources (organic and inorganic), inclusion levels, two levels for the inorganic source (0.30 and 0.60ppm) and four for the organic source (0.15, 0.30, 0.45 and 0.60ppm) and one control diet without Se supplementation containing 0.095ppm, and six replicates of three animals per pen. The experimental period was 35 days post-weaning. The organic source contained...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme activity; Glutathione peroxidase; Hepatic selenium; Plasmatic selenium.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200653
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Differential survivorship of congeneric ornamental fishes under forecasted climate changes are related to anaerobic potential Genet. Mol. Biol.
Gonçalves,Luciana Mara Fé; Silva,Maria de Nazaré Paula da; Val,Adalberto Luis; Almeida-Val,Vera Maria Fonseca de.
Abstract Two Amazonian closely related tetras – cardinal Paracheirodon axelrodi and green neon P. simulans – were artificially acclimatized to environmental chambers mimicking future climate change scenarios (mild, moderate and extreme), using a microcosm facility. P. simulans survived (100%) to all scenarios after 30 days exposure, while P. axelrodi presented decreasing survival percentages according to environmental severity. These differences may be the reflection of distinct natural acclimatization to microhabitats between the species, which differ in thermal conditions. Survival responses might be related to differences in relative gene expression of lactate dehydrogenase (Ldh), suggesting that P. axelrodi anaerobic potential is lower or non-existent...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ornamental fish; Enzyme activity; Relative gene expression; IPCC scenarios; Lactate Dehydrogenase (Ldh).
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100107
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Diurnal changes in storage carbohydrate metabolism in cotyledons of the tropical tree Hymenaea courbaril L. (Leguminosae) Rev. Bras. Bot.
Amaral,Lourdes Isabel Velho do; Santos,Henrique P.; Rossatto,Davi Rodrigo; Buckeridge,Marcos Silveira.
The cotyledons of Hymenaea courbaril store large amounts of xyloglucan, a cell wall polysaccharide that is believed to serve as storage for the period of seedling establishment. During storage mobilisation, xyloglucan seems to be degraded by a continuous process that starts right after radicle protrusion and follows up to the establishment of photosynthesis. Here we show evidence that events related to the hydrolases activities and production (α-xylosidase, β-galactosidase, β-glucosidase and xyloglucan endo-β-transglucosilase) as well as auxin, showed changes that follow the diurnal cycle. The period of higher hydrolases activities was between 6pm and 6am, which is out of phase with photosynthesis. Among the enzymes, α-xilosidase seems to be more important...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme activity; IAA; Α-xylosidase; XTH; Xyloglucan.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042012000400008
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Effect of growth hormone on fatty acid synthase gene expression in porcine adipose tissue cultures Genet. Mol. Biol.
José,Andrea A.F.B.V.; Alvares,Lúcia. E.; Delgado,Eduardo F.; Coutinho,Luiz L.; Lanna,Dante P.D..
We describe an efficient in vitro assay to test growth hormone effects on mRNA levels and fatty acid synthase (FAS, EC. 2.3.1.85) activity. Swine adipose tissue explants were long-term cultured in medium containing growth hormone and FAS mRNA levels and enzyme activity were measured. We quantified FAS transcripts by competitive reverse transcriptase PCR (RT-PCR) using total RNA from cultured adipose tissue explants and RT-PCR standard-curves were constructed using a cloned 307 bp segment of native FAS cDNA and a shorter fragment from which a 64 bp (competitor, 243 bp) internal sequence had been deleted. A known amount of competitor was added to each PCR as an internal control and µ-actin transcripts were also measured to correct for differences in total...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MRNA quantification; Enzyme activity; Swine; Cultures of adipose tissue explants; Competitive RT-PCR.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100032
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Effect of Non-Starch Polysaccharide (NSP) of Wheat and Barley Supplemented with Exogenous Enzyme Blend on Growth Performance, Gut Microbial, Pancreatic Enzyme Activities, Expression of Glucose Transporter (SGLT1) and Mucin Producer (MUC2) Genes of Broiler Chickens Rev. Bras. Ciênc. Avic.
Yaghobfar,A; Kalantar,M.
ABSTRACT The experiment was carried out in a completely randomized design (CRD) with 625 broiler chicks (Ross 308) for 5 repetitions (25 birds per each replicated) on the 5 treatments diet. Treatments included two different types of cereal grains (wheat, and barley) with or without an enzyme supplementation along with a corn-based diet as control group. The experimental diets were formulated to have similar contents of crude protein, metabolizable energy, total non-starch polysaccharides (NSP) and were fed in two periods of starter and grower. Experimental traits were consisted growth performance, ileal flora numeration, villus morphology in 3 parts of the intestine, digesta viscosity and pancreatic enzyme activity, and determining the gene expression...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broilers; Enzyme activity; Gene expression; Micro flora; NSP.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000400629
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Endophytic fungi producing of esterases: evaluation in vitro of the enzymatic activity using pH indicator BJM
Lisboa,Helen Cristina Fávero; Biasetto,Carolina Rabal; Medeiros,João Batista de; Araújo,Ângela Regina; Silva,Dulce Helena Siqueira; Teles,Helder Lopes; Trevisan,Henrique Celso.
A sensitive and efficient colorimetric method was optimized for detection of esterase enzymes produced by endophytic fungi for development of High-Throughput Screening (HTS). The fungi were isolated and obtained previously from plant species of Cerrado and Atlantic Forest located in areas of environmental preservation in the State of Sao Paulo / Brazil, as part of the project "Chemical and biological prospecting endophytic fungi associated to plant species of Cerrado and Atlantic Forest". The compounds ethyl butyrate, ethyl acetate and methyl propionate were used as standards esters which were hydrolyzed by extracellular enzyme from endophytic fungi (EC. 3.1.1.1 -carboxylesterases) for production of carboxylic acids. Thus, the reduction of the pH increases...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Esterase; PH indicator; Enzyme activity; Endophytic fungi.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300042
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Ethylene and lettuce seed germination Scientia Agricola
Nascimento,Warley Marcos.
Ethylene can stimulate seed germination and overcome dormancy in many species. For instance, the inhibitory effect of high temperature on lettuce seed germination can be overcome by exogenous ethylene. Involvement of ethylene in seed germination is a widely accepted fact, but the mechanistic details are poorly understood. A critical factor in ethylene studies is the response of seed germination properties to various ethylene inhibitors. Lettuce seed has been used as a model to study the role of ethylene in seed germination at high temperature. The role of ethylene in germination is not known but either ethylene evolution or addition of 1-aminocyclopropane-1-carboxilic acid (ACC) have been associated with increased activity of endo-beta-mannanase, a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactuca sativa; Thermoinhibition; Dormancy; Enzyme activity.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000300029
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Histological and Histochemical Studies on the Effects of Methotrexate on the Liver of Adult Male Albino Rat International Journal of Morphology
Al-Motabagani,Mohamed Akram.
Methotrexate (MTX) is widely used in the therapy of various types of malignancy. The present work was designed to investigate the histological and histochemical changes in the liver of albino rat following methotrexate administration. Fifteen adult male albino rats were used in the present work. They were divided into three main groups: Group I was kept without treatment and served as control. Groups II and III were given intraperitoneal injections of normal saline and MTX, respectively, at a dosage of (0.5 mg/Kg) twice weekly for total durations of 3, 6 and 9 weeks. The rats were sacrificed and the livers were excised and processed for histological and histochemical study. Examination of sections of the livers of group III showed mononuclear cell...
Tipo: Journal article Palavras-chave: Methotrexate; Hepatocytes; Hepatic lobules; Lycogen; Enzyme activity.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400020
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