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| Registros recuperados: 41 | |
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| Bressan,Fabiana Fernandes; Fantinato-Neto,Paulo; Andrade,Gabriella Mamede; Sangalli,Juliano Rodrigues; Sampaio,Rafael Vilar; Silveira,Juliano Coelho da; Perecin,Felipe; Meirelles,Flávio Vieira. |
| This review aim to present some clinical problems found in IVP-derived animals focusing on NT procedures and to discuss the possible role of epigenetics in such process. Also, as cell-secreted vesicles have been reported as possible regulators of important physiological reproductive processes such as folliculogenesis and fertilization, it is also presented herein a new perspective of manipulating the pre-implantation period trough effector molecules contained in such vesicles. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo development; Nuclear transfer; Epigenetics; Extra-cellular vesicles.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001001879 |
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| Sui,W.G.; He,H.Y.; Yan,Q.; Chen,J.J.; Zhang,R.H.; Dai,Y.. |
| Membranous nephropathy (MN), characterized by the presence of diffuse thickening of the glomerular basement membrane and subepithelial in situimmune complex disposition, is the most common cause of idiopathic nephrotic syndrome in adults, with an incidence of 5-10 per million per year. A number of studies have confirmed the relevance of several experimental insights to the pathogenesis of human MN, but the specific biomarkers of MN have not been fully elucidated. As a result, our knowledge of the alterations in histone methylation in MN is unclear. We used chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) to analyze the variations in a methylated histone (H3K9me3) in peripheral blood mononuclear cells from 10 MN patients and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ChIP-seq; Epigenetics; H3K9me3; Membranous nephropathy. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100042 |
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| Cheray, Mathilde; Etcheverry, Amandine; Jacques, Camille; Pacaud, Romain; Bougras-cartron, Gwenola; Aubry, Marc; Denoual, Florent; Peterlongo, Pierre; Nadaradjane, Arulraj; Briand, Josephine; Akcha, Farida; Heymann, Dominique; Vallette, François M.; Mosser, Jean; Ory, Benjamin; Cartron, Pierre-françois. |
| Background Literature reports that mature microRNA (miRNA) can be methylated at adenosine, guanosine and cytosine. However, the molecular mechanisms involved in cytosine methylation of miRNAs have not yet been fully elucidated. Here we investigated the biological role and underlying mechanism of cytosine methylation in miRNAs in glioblastoma multiforme (GBM). Methods RNA immunoprecipitation with the anti-5methylcytosine (5mC) antibody followed by Array, ELISA, dot blot, incorporation of a radio-labelled methyl group in miRNA, and miRNA bisulfite sequencing were perfomred to detect the cytosine methylation in mature miRNA. Cross-Linking immunoprecipiation qPCR, transfection with methylation/unmethylated mimic miRNA, luciferase promoter reporter plasmid,... |
| Tipo: Text |
Palavras-chave: MiRNA; Cytosine-methylation; Epigenetics; DNMT3A; AGO4; Glioblastoma; Epitranscriptomics. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00610/72196/70980.pdf |
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| Rodrigues,Maria Gabriela Fontanetti; Soares,Murilo Racy; Gimenez,Daniele Fernanda Jovino; Fonseca,Larissa Fernanda Simielli; Torrieri,Erico; Ramos,Ester Silveira; Giuliatti,Silvana. |
| ABSTRACT Fig tree (Ficus carica L.) breeding programs using conventional methods, such as directed crosses, to obtain new cultivars, are unworkable in many countries, including Brazil. Consequently, genetic breeding through mutagenesis has emerged as an important line of research that can improve this crop, and be a significant source of information about this species and assist in the implementation of propagation projects and appropriate management. The aim of this study was to verify the existence of epigenetic variability attributable to DNA methylation in irradiated fig selections when compared both to each other and to the main commercial cultivar, “Roxo-de-Valinhos”, which had previously used methylation-sensitive amplified polymorphism (MSAP) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ficus carica; DMRs; Methylation; Plant breeding; Epigenetics. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000400285 |
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| CAETANO, L. C.; GENNARO, F. G. O.; COELHO, K.; ARAÚJO, F. M.; VILA, R. A.; ARAÚJO, A.; BERNARDO, A. de M.; MARCONDES, C. R.; LOPES, S. M. C. de S.; RAMOS, E. S.. |
| The chicken (Gallus gallus) embryo has been used as a classic model system for developmental studies because of its easy accessibility for surgical manipulation during embryonic development. Sex determination in birds is chromosomally based (ZZ for males and ZW for females); however, the basic mechanism of sex determination is still unknown. Here, the dynamics of expression of candidate genes implicated in vertebrate sex determination and differentiation were studied during embryonic chicken gonadal development. Gene expression profiles were obtained before, during, and after gonadal sex differentiation in females and males for DMRT1, SOX3, SOX9, DAX MHM and sex-determining gene expression in chicken embryos SCII, HINTZ, HINTW, and the male hypermethylated... |
| Tipo: Artigo em periódico indexado (ALICE) |
Palavras-chave: Gonad; Development; Chicken; Epigenetics; Dosage compensation. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/handle/doc/985036 |
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| Andrade,F.O.; Nagamine,M.K.; Conti,A. De; Chaible,L.M.; Fontelles,C.C.; Jordão Junior,A.A.; Vannucchi,H.; Dagli,M.L.Z.; Bassoli,B.K.; Moreno,F.S.; Ong,T.P.. |
| The combined treatment with histone deacetylase inhibitors (HDACi) and retinoids has been suggested as a potential epigenetic strategy for the control of cancer. In the present study, we investigated the effects of treatment with butyrate, a dietary HDACi, combined with vitamin A on MCF-7 human breast cancer cells. Cell proliferation was evaluated by the crystal violet staining method. MCF-7 cells were plated at 5 x 10(4) cells/mL and treated with butyrate (1 mM) alone or combined with vitamin A (10 µM) for 24 to 120 h. Cell proliferation inhibition was 34, 10 and 46% following treatment with butyrate, vitamin A and their combination, respectively, suggesting that vitamin A potentiated the inhibitory activities of butyrate. Furthermore, exposure to this... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breast cancer; MCF-7 cells; Butyric acid; Vitamin A; Epigenetics. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000900008 |
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| Anselmo,Nilson Praia; Bello,Maria Josefa; Gonzalez-Gomez,Pilar; Dias,Luis Antonio Araújo; Almeida,José Reinaldo Walter de; Santos,Marcelo José dos; Rey,Juan A.; Casartelli,Cacilda. |
| Aberrant methylation of CpG islands located in promoter regions represents one of the major mechanisms for silencing cancer-related genes in tumor cells. We determined the frequency of aberrant CpG island methylation for several tumor-associated genes: DAPK, MGMT, p14ARF, p16INK4a, TP73, RB1 and TIMP-3 in 55 brain tumors, consisting of 26 neuroepithelial tumors, 6 peripheral nerve tumors, 13 meningeal tumors and 10 metastatic brain tumors. Aberrant methylation of at least one of the seven genes studied was detected in 83.6% of the cases. The frequencies of aberrant methylation were: 40% for p14ARF, 38.2% for MGMT, 30.9% for, p16INK4a, 14.6% for TP73 and for TIMP-3, 12.7% for DAPK and 1.8% for RB1. These data suggest that the hypermethylation observed in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brain tumors; Epigenetics; Methylation. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300001 |
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| Fneich, Sara; Theron, Andre; Cosseau, Celine; Rognon, Anne; Aliaga, Benoit; Buard, Jerome; Duval, David; Arancibia, Nathalie; Boissier, Jerome; Roquis, David; Mitta, Guillaume; Grunau, Christoph. |
| Background: Adaptive evolution is not possible without the generation of phenotypic variants. The origin of these variations has been a central topic in evolutionary biology. Up to now, it was commonly accepted that standing genetic variation is the only cause of phenotypic variants. However, epigenetic information is emerging as a complementary source of heritable phenotypic variation that contributes to evolution. The relative importance of genetics and epigenetics in generating heritable phenotypic variation is nevertheless a matter of debate. Results: We used a host-parasite system to address this question. The human blood fluke Schistosoma mansoni can adapt rapidly to new intermediate snail hosts. The interaction between parasite and mollusk is... |
| Tipo: Text |
Palavras-chave: Epigenetics; Adaptive evolution; Compatibility polymorphism; Schistosoma mansoni. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00615/72708/71730.pdf |
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| Registros recuperados: 41 | |
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