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18S-rDNA SEQUENCING, ENZYME PATTERNS AND MORPHOLOGICAL CHARACTERIZATION OF TRICHOPHYTON ISOLATES BJM
Nascimento,Adriana Mendes do; Martinez-Rossi,Nilce M..
Dermatophytes, capable to use keratin of the host for nutrition, belong to one of the major groups of pathogenic fungi. Since dermatophytes are a closely related group they share various common features, and the morphology of isolates of a given species can be atypical, making species identification and differentiation even more difficult. Many methods have been explored in attempts to distinguish dermatophytes, but the combined use of different approaches for the investigation of the intraspecific and interspecific variability of Trichophyton continues to be scarce. Some studies have shown that amplified fragments of the small ribosomal DNA subunit 18S contains variable regions which can be used to discriminate between medically relevant yeast species,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichophyton; 18S-rDNA sequencing; Extracellular enzymes; Dermatophyte.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300004
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Antagonism of Trichoderma species on Cladosporium herbarum and their enzimatic characterization BJM
Barbosa,Maria Angélica G.; Rehn,Kurt Georg; Menezes,Maria; Mariano,Rosa de Lima R..
The verrucose caused by Cladosporium herbarum reduces production and quality of Passion fruit (Passiflora edulis Sims.), a largely consumed tropical fruit. This work aimed to investigate the antagonism of Trichoderma species (T. polysporum, T. koningii, T. viride and T. harzianum) against Cladosporium herbarum, and to study the production of extracellular hydrolytic enzymes by the pathogen and the antagonists. The results showed considerable antagonistic potential for the biocontrol of C. herbarum isolates by all Trichoderma species, except T. koningii. The most distinguished effect was observed for T. polysporum. In relation to the pattern of esterase obtained by electrophoresis in poliacrylamyde gel, the major activity presented by the isolates was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Passion fruit; Biological control; Extracellular enzymes; Fungi.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200005
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Polyphasic characterization of endophytic bacteria of sorghum grown on cerrado soil of the Goiás State - Brazil. Repositório Alice
SILVA, L. B. da; SILVA, L. L. da; FERREIRA, E. P. de B.; SANTOS, K. F. E. N.; MARTIN-DIDONET, C. C. G..
Sorghum is a cereal of great commercial importance adapted to the tropical and subtropical regions. Isolation of plant-associated bacteria of agronomic interest has been the subject of several research works, however, there are few studies on bacteria isolating from sorghum. The objective of this work was to characterize as plant growth promoters endophytic bacteria isolated from sorghum roots. A total of 24 isolates were selected using the NFb-lactate, LGI-P, and YM semi-selective medium. The characterization was based on the resistance to antibiotics, use of carbon sources, enzyme activity and 16S-23S intergenic analysis. The isolates were more sensitive to the antibiotic streptomycin and showed a higher capacity to produce lipases and cellulases. Carbon...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Extracellular enzymes; Phosphate solubilization; Endophyticmicroorganisms; Sorgo; Solo; Cerrado; Bactéria; Sorghum; Phosphates.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1078151
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Presence of intra and extracellular enzymes in strains of Acinetobacter spp, produced in different inducers. Repositório Alice
SCRAMIN, S.; ROQUE, M. R. A.; DENTZIEN, A. F. M.; ROSSO, C. R. S. de; MELO, I. S. de.
Two Acinetobacter spp. strains, denominated D-12 and D-16 (extracted from Diuron TM treated sugarcane rhizosphere) were grown in different substrates in order to determine whether catechol 1,2-dioxygenase (pyrocatechase) and catechol 2,3-dioxygenase (metapyrocatechase) are constitutive or induced enzymes. Extracellular enzymes were also measured. Although biomass was measured, the conditions required for best production of microorganisms were not established. Five different inducers were added to culture media: glucose, sodium benzoate, Diuron TM, dichloroaniline and Diuron TM plus glucose. Both strains were harvested by centrifugation and disrupted by sonication for intracellular enzyme extraction. Extracellular enzyme assays were carried out with...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Extracellular enzymes; Acinetobacter spp.
Ano: 1998 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/12899
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