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Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content Electron. J. Biotechnol.
Wang,Chuan Tang; Tang,Yue Yi; Wang,Xiu Zhen; Zhang,Shu Wei; Li,Gui Jie; Zhang,Jian Cheng; Yu,Shan Lin.
Screening of peanut seeds resulting from 0.39% sodium azide treatment with NIRS calibration equation for bulk seed samples identified a plant with more than 60% oleate. Oleate content in individual seeds of the plant, as predicted by NIRS calibration equation for intact single peanut seeds, ranged from 50.05% ~ 68.69%. Three seeds with >60% oleate thus identified were further confirmed by gas chromatography. Multiple sequence alignments of the FAD2B gene from Huayu 22 (wild type) and peanut seeds with elevated oleate (mutant type) revealed a C281T transition in the coding region causing an I94T substitution in the oleoyl-PC desaturase, which may be responsible for reduction in the enzyme activity.
Tipo: Journal article Palavras-chave: Chemical mutant; FAD2B; NIRS; Oleate; Peanut.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200010
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