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Registros recuperados: 27
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Decreased spermatogenic and androgenic testicular functions in adult rats submitted to immobilization-induced stress from prepuberty BJMBR
Almeida,S.A.; Petenusci,S.O.; Anselmo-Franci,J.A.; Rosa-e-Silva,A.A.M.; Lamano-Carvalho,T.L..
We investigated whether chronic stress applied from prepuberty to full sexual maturity interferes with spermatogenic and androgenic testicular functions. Male Wistar rats (40 days old) were immobilized 6 h a day for 60 days. Following immobilization, plasma concentrations of corticosterone and prolactin increased 135% and 48%, respectively, while plasma luteinizing hormone and testosterone presented a significant decrease of 29% and 37%, respectively. Plasma concentration of follicle-stimulating hormone was not altered in stressed rats. Chronic stress reduced the amount of mature spermatids in the testis by 16% and the spermatozoon concentration in the cauda epididymidis by 32%. A 17% reduction in weight and a 42% decrease in DNA content were observed in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Spermatogenesis; LH; FSH; Testosterone; Prolactin.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100013
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Efeito do intervalo entre recrutamentos foliculares na superovulação de vacas da raça Holandesa não-lactantes Arq. Bras. Med. Vet. Zootec.
Santos,R.M.; Vasconcelos,J.L.M..
Vacas da raça Holandesa não-lactantes, distribuídas em dois grupos, foram sincronizadas com o protocolo Ovsynch modificado. No dia sete (dia 0 = dia do segundo GnRH), o grupo 7 (G-7; n=19) recebeu CIDR usado previamente por cinco dias e 100mcg de GnRH, e o grupo 14 (G-14; n=21), CIDR e 25mg de PGF2alfa. No dia 14 foi aspirado o folículo dominante (FD), trocado o CIDR usado por um novo e foram aplicados 25mg de PGF2alfa. Iniciou-se o tratamento com FSH 36h depois, removeu-se o CIDR com o sétimo FSH e aplicou-se GnRH 36h depois. As inseminações foram feitas 12 e 24h depois. Recuperaram-se os embriões sete dias depois da inseminação artificial. O diâmetro do FD no G-7 foi 13,1±0,57mm no dia sete e 11,2±0,57mm no dia 14. O diâmetro FD persistente no G-14...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovino; Aspiração folicular; Embrião; FSH; Superovulação.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000400004
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Effect of Bone Morphogenetic Protein 6 (BMP6) on Chicken Granulose Cells Proliferation and Progesterone Synthesis Rev. Bras. Ciênc. Avic.
Yang,F; He,L; Luo,B; Ye,F; Cui,C; Yu,X; Xu,H; Zhao,X; Yin,H; Li,D; Zhu,Q; Wang,Y.
ABSTRACT There is increasing evidence that bone morphogenetic protein 6 (BMP6) plays critical roles in regulating various stages of ovarian follicle development in mammals. However, the mechanisms of regulation of BMP6 in the chicken ovary remain unclear. In this study, mRNA and protein expression level of BMP6 in chicken ovarian follicles at different development stages were determined by qRT-PCR and western blot separately. Different concentrations of BMP6 protein and FSH were added to the culture medium, and the effects to proliferation of granulose cells were detected, further effect on expression pattern of progesterone synthesis associated genes were also analyzed by qRT-PCR and Western blotting and the secretion of progesterone was detected by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken; BMP6; Granulosa cells; Progesterone; FSH.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200310
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Effect of charcoal-treated bovine follicular fluid on the secretion of FSH in ovariectomized and intact prepubertal heifers Braz. J. Vet. Res. Anim. Sci.
ALVAREZ,Rafael Herrera; ARCARO,Juliana Rodrigues Pozzi; RIBELA,Maria Teresa Carvalho Pinto; PERONE,Cibele Nunes.
The aim of the experiment was to determine whether charcoal-treated bovine follicular fluid (bFF) removed from visible (< 22 mm) follicles altered the secretion of follicle-stimulating hormone (FSH) in intact and ovariectomized prepubertal heifers. After a 10-ml injection of bFF given at three consecutive 8-h intervals, secretion of FSH was depressed in approximately 44% of the ovariectomized heifers but there was no effect in intact heifers. When the bFF treatment ceased, there were no rebound effects on FSH concentrations above that of controls pretreatment levels. These results suggest that proteins from bFF act at the pituitary level to inhibit FSH secretion and, differently of the intact, the ovariectomized heifer is an adequate model to put this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Follicular fluid; FSH; Bovine.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961998000500004
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Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats BJMBR
Follicle-stimulating hormone (FSH) and insulin regulate glycide metabolism in Sertoli cells, thus stimulating lactate production. These stimulatory effects of FSH and insulin do not require protein synthesis, suggesting a modulation of enzyme activity and/or regulation of glucose transport. The present investigation was performed to characterize the hormonal control of lipid metabolism in Sertoli cells. The data indicate that FSH and insulin have a regulatory effect on lipid metabolism in Sertoli cells. After 8 h of preincubation with insulin (5 µg/ml), the activity of the enzyme ATP-citrate lyase in cultured Sertoli cells was increased from 0.19 to 0.34 nmol NAD+ formed µg protein-1 min-1. FSH (100 ng/ml) had no effect on this enzyme. Glycerol phosphate...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Sertoli cells; Lipids; FSH; Insulin.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500004
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Effect of the dry matter intake level on the sanguine profile of glucose, insulin, urea, estrogen and progesterone and concentration of IGF-I in the follicular liquid of crossbred heifers BABT
Rigolon,Luiz Paulo; Prado,Ivanor Nunes do; Cavalieri,Fábio Luiz Bim; Nascimento,Willian Gonçalves do; Copovila,Luiz Carlos; Ramos,Fabíola dos Santos; Moreira,Fernanda Barros.
The aim of this work was to study effect of the dry matter intake level on the concentrations of glucose, insulin, urea, estrogen and progesterone in the blood and the level of IGF-I in the follicular liquid of heifers. Twenty seven crossbred heifers were used, in three treatments: 1.2, 1.6 and 2.6% of the live weight of dry matter intake (DMI) daily. The sanguine concentrations of glucose and estrogen were higher in the animals with 1.6% of DMI and the insulin levels were higher in the animals with 2.6% of DMI. There was an increase in the sanguine levels of urea with the increase of the DMI. There was no effect of the DMI on the progesterone levels in the sanguine plasma collected from the jugular. The progesterone levels in caudal vena were higher in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: FSH; Hormones; Intake; Nutrition; Reproduction; Superovulation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100008
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Follicle profile and plasma gonadotropin concentration in pubertal female ponies BJMBR
Nogueira,G.P..
Twelve female ponies were examined daily for 30 days and classified as ovulating (OV; N = 6; 197 ± 6 kg) or prepubertal (PP; N = 6; 196 ± 9 kg). Follicles were detected by ultrasound and gonadotropins quantified by radioimmunoassay. The mean diameter of the largest follicles was significantly larger in OV (38 ± 1 mm) than in PP (26 ± 2 mm) but there was no difference between groups in the size of the second largest follicle. There were more small follicles (<24 mm) in the PP than in the OV group, but PP fillies had a smaller number of follicles >29 mm than the OV fillies. Follicle-stimulating hormone (FSH) levels did not differ between groups but PP fillies had lower luteinizing hormone (LH) peak (8 ± 1 ng/ml) and basal (4 ± 0.5 ng/ml) levels, lower...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Follicles; FSH; LH and prepubertal fillies.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600018
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G protein-coupled receptor kinase 2 and beta-arrestins are recruited to FSH receptor in stimulated rat primary Sertoli cells Inra
Marion, S.; Kara, E.; Crepieux, P.; Piketty, V.; Martinat, N.; Guillou, F.; Reiter, E..
Tipo: Journal Article Palavras-chave: CELLULE DE SERTOLI; FSH; RECEPTEUR; ARRESTINE; PROTEINE KINASE; PROTEINE G.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200715f36fec&uri=/notices/prodinra1/2008/01/
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Genetic relationship between prepuberal plasma FSH levels and reproductive performance in Lacaune ewe lambs Inra
Bodin, L.; Bibe, B.; Blanc, M.R.; Ricordeau, G..
Cette étude estime les relations phénotypiques et génétiques entre le taux plasmatique impubère de FSH (et logFSH) des agnelles Lacaune viande et leurs premières performances de reproduction (fertilité et prolificité). Les niveaux hormonaux sont estimés à partir d’un seul échantillon de sang prélevé à environ 5 semaines d’âge (moyenne ± écart type = 33.7 ± 3.2 jours) sur 829 jeunes agnelles nées de juillet à septembre 1983, dans 30 élevages, et issues de 33 pères utilisés en insémination. La lutte a lieu en deux périodes, en début de saison sexuelle à 11 mois, et en octobre-novembre à 15 mois. L’analyse de la fertilité concerne uniquement les agnelles mises en lutte à la première période (n = 737), alors que l’analyse de la prolificité porte sur...
Tipo: Journal Article Palavras-chave: FSH; FERTILITE; CORRELATION GENETIQUE.
Ano: 1988 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9300034091029124&uri=/notices/prodinra1/2008/01/
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Impact of pituitary FSH purification on in vitro early folliculogenesis in goats Biocell
Magalhães,D.M.; Araújo,V.R.; Lima-Verde,I.B.; Matos,M.H.T.; Silva,R.C.; Lucci,C.M.; Báo,S.N.; Campello,C.C.; Figueiredo,J.R..
Porcine pituitary follicle stimulating hormone (pFSH) is known to regulate the production of growth factors that have an essential role in early foliculogenesis. However, the effects of different preparations of pFSH on the survival and development of caprine follicles are not yet known. The aim of this study was to evaluate the effects of different pFSH (Stimufol® and Folltropin®) on the in vitro survival and growth of caprine preantral follicles. Pieces of caprine ovarian tissues were cultured for either one or seven days in a supplemented Minimum Essential Medium, alone or containing either Stimufol® (50 ng/mL) or Folltropin® (10, 50, 100 and 1000 ng/mL). Fresh control ovarian tissues as well as cultured tissued were processed for histological and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Goat; Preantral follicles; FSH; Follicular growth.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200003
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Influência do folículo dominante sobre a dinâmica folicular ovariana em vacas Nelore tratadas com FSH Braz. J. Vet. Res. Anim. Sci.
GRADELA,Adriana; MALHEIROS,Ramon; URBINATTI,Elizabeth Crisciolo; BARBOSA,José Carlos; ALMEIDA JR,Ivo Luís de; ESPER,César Roberto.
Os objetivos deste estudo foram: 1) determinar o diâmetro máximo e a taxa de crescimento do folículo dominante (FD) em vacas Nelore; 2) determinar o intervalo entre a administração de PGF2<FONT FACE="Symbol">µ</FONT> e as manifestações de estro; 3) investigar a influência da remoção do FD sobre o número de corpos lúteos (CL) e folículos não ovulados (FNO) determinados por ultra-sonografia (CLus e FNOus); e 4) determinar as concentrações de progesterona (P4, ng/ml) e estradiol-17beta (E2, pg/ml) em vacas Nelore submetidas a superovulação com FSH. Após os exames ultra-sonográficos no D0 (48 horas antes do início da superovulação), 42 vacas Nelore foram divididas em 3 grupos experimentais: GI (sem FD, n = 15), GII (com FD, n = 15), e GIII (com FD...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Folículo ovariano; FSH; Gado Nelore; Ultra-sonografia.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000400013
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MADURACIÓN in vitro DE OVOCITOS DE OVINO USANDO CONCENTRACIONES DE FSH+LH Y FSH EN MEDIO DE CULTIVO Rev Salud Anim.
Fernández Reyes,F; Hernández Pichardo,J.E; Pichardo Cruz,A.I.
Con el objetivo de lograr la maduración in vitro de ovocitos ovinos, se obtuvieron ovarios a partir de ovinos sacrificados. Inmediatamente después del sacrificio los ovarios se sumergieron en solución salina fisiológica a 35ºC. Se colectaron en total 242 ovarios y se debridaron 1294 folículos con un tamaño entre 2 a 6 mm de diámetro. Se seleccionaron 107 ovocitos que presentaron 3 ó más capas compactas de células del cúmulo y citoplasma homogéneo, después fueron cultivados en medio de maduración TCM 199 suplementado con FSH y LH en concentraciones: 1) 5 mg/mL de FSH y 5 mg/mL de LH (22 ovocitos), 2) 2.5 mg/mL de FSH y 2.5 mg/mL de LH (16 ovocitos), 3) 5 mg/mL de FSH (36 ovocitos), 4) 2.5 mg/mL de FSH (33 ovocitos). Posteriormente fueron fijados y evaluados...
Tipo: Journal article Palavras-chave: Maduración in vitro; Ovocitos; Ovino; FSH; LH.
Ano: 2007 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2007000200007
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Mathematical model of FSH-induced cAMP production in ovarian follicles Inra
Clément, F.; Monniaux, D.; Stark, J.; Hardy, K.; Thalabard, J.C.; Franks, S.; Claude, D..
Tipo: Journal Article Palavras-chave: AMP-C; FSH; FOLLICULE OVARIEN; REPRODUCTION; ADENOSINE MONOPHOSPHATE CYCLIQUE.
Ano: 2001 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0300034006097357&uri=/notices/prodinra1/2010/09/
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Modelling of FSH signalling network in ovarian follicle cells with BIOCHAM Inra
Clément, F.; Fages, F.; Heitzler, D.; Reiter, E..
Tipo: Meeting Paper Palavras-chave: FOLLICULE OVARIEN; FSH.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20081eda037b&uri=/notices/prodinra1/2008/01/
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Neuroendocrinologie de la reproduction chez les caprins Inra
Chemineau, P.; Delgadillo, J.A..
L’hypothalamus, par l’intermédiaire de la sécrétion du LH-RH, commande la libération épisodique des hormones gonadotropes LH et FSH dans la circulation générale. La LH n’est pas sécrétée de façon continue par l’hypohyse mais sous forme de "pulses", définis par leur fréquence et leur amplitude, qui stimulent la libération de la testostérone par le testicule chez le mâle et de l’oestradiol et de la progestérone par l’ovaire chez la femelle. La FSH est sécrétée de façon moins épisodique. Chez la chèvre, la modulation des rétroactions négatives et positives de la progestérone et de l’oestradiol sur l’axe hypothalamo-hypohysaire joue un rôle clé dans la régulation des différents événements du cycle oestrien. Une imprégnation par la progestérone n’est pas...
Tipo: Journal Article Palavras-chave: REPRODUCTION; VARIATION SAISONNIERE; LH; FSH; SYSTEME NERVEUX CENTRAL; PHOTOPERIODISME.
Ano: 1994 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9500034106048994&uri=/notices/prodinra1/2008/02/
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Percutaneous 17ß-estradiol replacement therapy in hypertensive postmenopausal women BJMBR
Osório-Wender,M.C.; Vitola,D.; Spritzer,P.M..
The present study evaluated the short-term effects of percutaneous 17ß-estradiol on blood pressure, metabolic profile and hormonal levels in postmenopausal women with systemic arterial hypertension. After a wash-out period of 15 days, 10 hypertensive patients were treated with guanabenz acetate to control blood pressure, followed by 17ß-estradiol in the form of hydroalcoholic gel administered for 21 of 28 days of each cycle, for 3 cycles. Patients were evaluated before, during and 2 months after estrogen administration. Systolic and diastolic blood pressure or heart rate did not present any significant change in any patient when compared to those periods with the antihypertensive drug only (pretreatment period and 60 days after estrogen therapy was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Menopause; Hormone replacement therapy; Hypertension; 17ß-estradiol; LH; FSH.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900001
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Possible involvement of A1 receptors in the inhibition of gonadotropin secretion induced by adenosine in rat hemipituitaries in vitro BJMBR
Picanço-Diniz,D.L.W.; Valença,M.M.; Favaretto,A.L.V.; McCann,S.M.; Antunes-Rodrigues,J..
We investigated the participation of A1 or A2 receptors in the gonadotrope and their role in the regulation of LH and FSH secretion in adult rat hemipituitary preparations, using adenosine analogues. A dose-dependent inhibition of LH and FSH secretion was observed after the administration of graded doses of the R-isomer of phenylisopropyladenosine (R-PIA; 1 nM, 10 nM, 100 nM, 1 µM and 10 µM). The effect of R-PIA (10 nM) was blocked by the addition of 8-cyclopentyltheophylline (CPT), a selective A1 adenosine receptor antagonist, at the dose of 1 µM. The addition of an A2 receptor-specific agonist, 5-N-methylcarboxamidoadenosine (MECA), at the doses of 1 nM to 1 µM had no significant effect on LH or FSH secretion, suggesting the absence of this receptor...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adenosine; LH; FSH; A1 receptor; Anterior pituitary.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900017
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Pregnancy rate in lactating Bos indicus cows subjected to fixed-time artificial insemination and treated with different follicular growth inducers R. Bras. Zootec.
Nogueira,Ériklis; Batista,Dayanna Schiavi do Nascimento; Costa Filho,Luiz Carlos Cesar da; Dias,Alexandre Menezes; Silva,Juliana Corrêa Borges; Ítavo,Luís Carlos Vinhas.
The objective of this experiment was to evaluate the effects of replacing eCG with FSH or FSH/LH on the diameter of ovulatory follicles (DOF) and the pregnancy rates (PR) of lactating Bos indicus cows subjected to a synchronization of ovulation protocol and fixed-time artificial insemination (FTAI) based on progesterone, estradiol benzoate (EB), PGF2α, and estradiol cypionate (EC). On day 0, primiparous and multiparous Nellore cows (n = 329) at 68 days postpartum were body condition scored (BCS) and received a P4 implant (PRIMER 1.0 g P4) plus 2 mg EB intramuscularly (IM). At day 8, the implant was removed, and 250 μg cloprostenol were injected IM along with 0.5 mg EC. At the time of implant removal, the animals were divided into one of four treatments:...
Tipo: Info:eu-repo/semantics/article Palavras-chave: FSH; Multiparous; Nellore; Primiparous.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000700358
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Produção de embriões em ovinos Morada Nova e Somalis Brasileira Arq. Bras. Med. Vet. Zootec.
Brasil,O.O.; Moreira,N.H.; Silva,T.A.S.N.; Silva,B.D.M.; Nascimento,N.V.; Facó,O.; Ramos,A.F..
ABSTRACT Superovulatory response and embryo yield in 19 Morada Nova and 20 Somalis Brasileira ewes was analyzed. All animals were synchronized with the insertion of an intravaginal device (CIDR(r)) on Day 0, replaced by a new device on Day 7, which remained in place until Day 14 and superovulated with 133mg of porcine FSH (pFSH) in decreasing doses at 12h intervals from Day 12 until Day 15 of the treatment, and a single dose of equine chorionic gonadotropin (eCG, 200UI) on Day 14 (i.e., administered in CIDR removal). Fifty hours after CIDR(r) removal, females were inseminated by laparoscopy. All embryos were recovered by laparotomy 5 days after insemination. Sheep which responded to the superovulation protocol (P>0.05) included 74% of the Morada Nova...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Superovulação; Embrião; FSH; Conservação de recursos genéticos.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501390
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Produção in vitro de embriões bovinos com soro de égua ou de vaca em estro com ou sem a adição de LH/FSH Ciência Rural
Figueiró,Giuliano Moraes; Leivas,Fábio Galas; Rauber,Lúcio Pereira; Sá Filho,Manoel Francisco de; Teichmann,Cristiane Elise; Mezzalira,Alceu; Rubin,Mara Iolanda Batistella; Silva,Carlos Antonio Mondino.
Mil duzentos e setenta e um oócitos foram divididos em 4 tratamentos com a finalidade de se avaliar a influência da adição de LH e FSH na produção in vitro de embriões bovinos com soro de vaca em estro (SVE) ou soro de éguas obtido no 1º dia do estro (SE). Independente do tratamento os oócitos foram maturados com TCM199 + 5,95mg/ml de Hepes, 0,025mg/ml de piruvato de sódio e 2,2mg/ml de bicarbonato de sódio, sendo adicionado 10% de soro de égua (ES), 10% de SVE (VS), 10% de soro de égua + 0,5mg/ml de hormônio luteinizante bovino (LHb) + 0,01UI/ml de hormônio folículo estimulante recombinante humano-rFSHh (EH) e 10% SVE + LHb + rFSHh (VH). Os oócitos assim tratados, foram maturados em estufa com 5% de CO2 a 39ºC sob umidade saturada por 22-24h. Depois,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embriões bovinos; LH; FSH; Soro de égua em estro; Soro de vaca em estro.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000200022
Registros recuperados: 27
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