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Registros recuperados: 8
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A hemocyanin-derived antimicrobial peptide from the penaeid shrimp adopts an alpha-helical structure that specifically permeabilizes fungal membranes ArchiMer
Petit, Vanessa W.; Rolland, Jean-luc; Blond, Alain; Cazevieille, Chantal; Djediat, Chakib; Peduzzi, Jean; Goulard, Christophe; Bachere, Evelyne; Dupont, Joelle; Destoumieux-garzon, Delphine; Rebuffat, Sylvie.
Background. Hemocyanins are respiratory proteins with multiple functions. In diverse crustaceans hemocyanins can release histidine-rich antimicrobial peptides in response to microbial challenge. In penaeid shrimp, strictly antifungal peptides are released from the C-terminus of hemocyanins. Methods. The three-dimensional structure of the antifungal peptide PvHCt from Litopenaeus vannamei was determined by NMR. Its mechanism of action against the shrimp pathogen Fusarium oxysporum was investigated using immunochemistry, fluorescence and transmission electron microscopy. Results. PvHCt folded into an amphipathic α-helix in membrane-mimicking media and displayed a random conformation in aqueous environment. In contact with F. oxysporum, PvHCt bound...
Tipo: Text Palavras-chave: Antimicrobial peptide; Amphipathic helix; Fungi; Membrane bilayer; Nuclear magnetic resonance (NMR); Fluorescence microscopy.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00301/41202/40362.pdf
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A study of autophagy in hemocytes of the Pacific oyster, Crassostrea gigas ArchiMer
Picot, Sandy; Morga, Benjamin; Faury, Nicole; Chollet, Bruno; Dégremont, Lionel; Travers, Marie-agnes; Renault, Tristan; Arzul, Isabelle.
Macroautophagy is a mechanism that is involved in various cellular processes, including cellular homeostasis and innate immunity. This pathway has been described in organisms ranging in complexity from yeasts to mammals, and recent results indicate that it occurs in the mantle of the Pacific oyster, Crassostrea gigas. However, the autophagy pathway has never been explored in the hemocytes of C. gigas, which are the main effectors of its immune system and thus play a key role in the defence of the Pacific oyster against pathogens. To investigate autophagy in oyster hemocytes, tools currently used to monitor this mechanism in mammals, including flow cytometry, fluorescent microscopy and transmission electron microscopy, were adapted and applied to the...
Tipo: Text Palavras-chave: Autophagosome; Autophagy; Crassostrea gigas; Flow cytometry; Fluorescence microscopy; Hemocytes; Transmission electron microscopy.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00487/59905/63087.pdf
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Comparaison de methodes directes (microscopiques) et indirectes (par mise en culture) dans l'evaluation d'une pollution bacterienne d'origine fecale. Etude preliminaire ArchiMer
Munro, P; Bianchi, M.
Cells of two serotypes of Escherichia coli (O26B6 and 0111B4) were counted using epifluorescence and immunofluorescence microscopy (nonavalent serum) and plate count on specific medium. Values from indirect counts were 10 fold smaller than those from both microscopic counts. In a sample of sea water from an area receiving a fecal pollution, the highest number of bacteria was obtained by epifluorescence microscopy. In that case, the number of cells counted by immunofluorescence microscopy was 10 fold smaller than the number of bacteria able to grow on the medium. It could be due to: the diversity of the natural community, the possibility to other bacteria than E. coli to grow on the used culture medium, the restrictiveness of the serum for "pathogenic...
Tipo: Text Palavras-chave: Escherichia coli; Bacteria; Microbiological culture; Pathogenic bacteria; Microbial contamination; Bacterial counters; Fluorescence microscopy; Bacteria collecting devices; Indicator species.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1001.pdf
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Fluorescent antibody enumeration of Vibrio cholerae in the marine environment. ArchiMer
Brayton, P; Roszak, D; Palmer, L; Huq, S; Gimes, D.
From microcosm studies, V. cholerae and related potential human pathogens enter a viable but nonculturable state. Direct viable counts by epifluorescent microscopy consistently remain higher than corresponding plate counts. Thus, the assumption that pathogens "die-off" or "decay" in the marine environment must be re-evaluated, since stressed or nutrient-starved cells are unable to grow and be enumerated by standard plate count methods. Indirect immunofluorescent microscopy offers a more sensitive detection system for environment sampling for human pathogens. Field indicate that cultures positive for V. cholerae O1 are also positive by fluorescent antibody staining; however, the reverse was not necessarily true. By adapting epifluorescent techniques for...
Tipo: Text Palavras-chave: Vibrio cholerae; Bacteria; Experimental research; Marine environment; Pathogenic bacteria; Microbial contamination; Fluorescence microscopy; Bacterial counters; Pollution detection.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1000.pdf
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In vitro Anticancer Property of Yellow Pigment fromStreptomyces griseoaurantiacus JUACT 01 BABT
Prashanthi,Kuruvalli; Suryan,Sandeep; Varalakshmi,Kilinger Nadumane.
ABSTRACT Despite the complications in isolation of pigments, microbial pigments are increasingly gaining the attention of researchers because of their broad range therapeutic potentials, especially against cancer. In this study the cytotoxic and anti proliferative potentials of yellow pigment from Streptomyces griseoaurantiacus JUACT 01 isolated from soil are investigated. The effect of pigment treatment on the growth and proliferation of in vitro cervical cancer cells (HeLa) and liver cancer cells (Hep G2) was tested by various methods. Significant cytotoxicity was observed with IC 50 values as low as 1.5 and 1.8 µg /mL with HeLa and Hep G2 cells respectively. The pigment exhibited non toxic effects on human lymphocytes. Decrease in the number of viable...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anticancer activity; ESI-MS; Fluorescence microscopy; Streptomyces griseoaurantiacus JUACT 01; Yellow pigment.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600869
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Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I Biocell
Varecha,Miroslav; Páclová,Daniela; Procházková,Jirina; Matula,Pavel; Cmarko,Dusan; Kozubek,Michal.
Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Superoxide production could be used as a valuable measure of complex I function, because the majority of superoxide is produced there. Therefore, we employed superoxide-specific mitochondrial fluorescence dye for detection of superoxide production. We studied an impact of AIF knockdown on function of mitochondrial complex I by analyzing superoxide production in selected cell lines. Our results show that tumoral telomerase-positive (TP)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Superoxide; Fluorescence microscopy; Image analysis; Mitochondria; Telomerase.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300004
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Techniques for sperm evaluation using fluorescent probes. Repositório Alice
CUNHA, A. T. M.; CARVALHO, J. de O.; DODE, M. A. N..
bitstream/item/135896/1/21231-107510-1-PB.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Sperm; Cryopreservation; Fluorescence microscopy.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1032028
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Variation analysis of bacterial polyhydroxyalkanoates production using saturated and unsaturated hydrocarbons BJM
Tufail,Saiqa; Munir,Sajida; Jamil,Nazia.
ABSTRACT Polyhydroxyalkanoates (PHA) are efficient, renewable and environment friendly polymeric esters. These polymers are synthesized by a variety of microbes under stress conditions. This study was carried out to check the suitability of waste frying oil in comparison to other oils for economical bioplastic production. Six bacterial strains were isolated and identified as Bacillus cereus (KF270349), Klebsiella pneumoniae (KF270350), Bacillus subtilis (KF270351), Brevibacterium halotolerance (KF270352), Pseudomonas aeruginosa (KF270353), and Stenotrophomonas rhizoposid (KF270354) by ribotyping. All strains were PHA producers so were selected for PHA synthesis using four different carbon sources, i.e., waste frying oil, canola oil, diesel and glucose....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Waste frying oil; Polyhydroxyalkanoates; Fluorescence microscopy; FTIR.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400629
Registros recuperados: 8
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