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Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 9
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A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses Biol. Res.
MEISEL,LEE; FONSECA,BEATRIZ; GONZÁLEZ,SUSANA; BAEZA-YATES,RICARDO; CAMBIAZO,VERONICA; CAMPOS,REINALDO; GONZALEZ,MAURICIO; ORELLANA,ARIEL; RETAMALES,JULIO; SILVA,HERMAN.
Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the...
Tipo: Journal article Palavras-chave: CDNA library; Fruit; Fruit trees; Functional genomics; Peach; Polysaccharides; Prunus persica; RNA isolation; Rosaceae; RT-PCR.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010
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Allocation du carbone et métabolisme azoté chez l'haptophyte Tisochrysis lutea ArchiMer
Garnier, Matthieu.
The aim of this thesis is to improve knowledge on mechanisms involved in the response to nitrogen limitation and in lipid accumulation in the microalgae haptophyte Tisochrysis lutea. The wild type strain and a lipid accumulating mutant strain were grown under different nitrogen limitation and starvation and analyzed by functional genomics. Four genes of high-affinity nitrate/nitrite transporter (Nrt2) were identified and characterized to reveal the mechanisms involved in mineral absorption in this species. Transcriptomes of both strains were sequenced and proteins affected by nitrogen starvation and differentially expressed between the two strains were identified. We so identified the functions regulated by nitrogen deficiency and potentially involved in...
Tipo: Text Palavras-chave: Microalgues; Métabolisme; Azote; Carbone; Lipides; Genomique fonctionelle; Protéomique; Transcriptomique; Microalgae; Metabolism; Nitrogen; Carbon; Lipids; Functional genomics; Proteomics; Transcriptomics.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00353/46378/46000.pdf
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Combined functional genomics and genetic approach in oyster to identify summer mortality-resistant markers ArchiMer
Huvet, Arnaud; Lapegue, Sylvie.
The oyster Crassostrea gigas has global distribution and for the past several years the highest annual production of any freshwater or marine organism. Economic importance of oysters motivates a great deal of biological research, which provides the most immediate scientific rationales for sequencing ESTs and developing genomic tools.
Tipo: Text Palavras-chave: Environmental genomics; Comparative genomics; Functional genomics; Markers; Summer mortalities; Genetic; Oysters; Crassostrea gigas.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4006.PDF
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Cracking in sweet cherries: A comprehensive review from a physiological, molecular, and genomic perspective Chilean J. Agric. Res.
Balbontín,Cristián; Ayala,Héctor; M. Bastías,Richard; Tapia,Gerardo; Ellena,Miguel; Torres,Carolina; Yuri,José Antonio; Quero-García,José; Ríos,Juan Carlos; Silva,Herman.
Rain-induced cracking in fruits of sweet cherry (Prunus avium L.) is a problem in most producing areas of the world and causes significant economic losses. Different orchard management practices have been employed to reduce the severity of this problem, although a complete solution is not yet available. Fruit cracking is a complex phenomenon and there are many factors that seem to be involved in its development. During the last decade, genomic and biochemical approaches have provided new insights on the different mechanisms that could be involved in the differential susceptibility shown by commercial cultivars. For instance, sweet cherry genome and transcriptome sequencing information have provided new opportunities to study the expression and structure of...
Tipo: Journal article Palavras-chave: Physiology; Functional genomics; RNA seq.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392013000100010
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Genomics and genetic transformation in arachis. Repositório Alice
BRASILEIRO, A. C. M.; ARAUJO, A. C. G.; LEAL BERTIOLI, S. C.; GUIMARAES, P. M..
2014
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Arachis; Functional genomics; Transgenic; Genome; Peanut.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1006434
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Global analysis of gene function in mammals: Integration of physical, mutational and expression strategies Electron. J. Biotechnol.
Schimenti,John.
The field of Genetics is undergoing tumultuous change after nearly a century of standard approaches to genetic analysis. The Human Genome Project is providing tools and technologies that are changing the ways that we pursue an understanding of gene function, which is the underlying goal in modern and traditional genetics. In this paper, I overview the directions of the genome project as they relate to gene function analysis in mice and humans, and how various modern technologies are coalescing to address this in a powerful way.
Tipo: Journal article Palavras-chave: Gene expression; ESTs; Mutagenesis; Genome; Functional genomics.
Ano: 1998 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581998000100005
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Rice genetics V: proceedings of the fifth International Rice Genetics Symposium, 19-23 Nov 2005. International Rice Research Institute
International Rice Genetics Symposium (5th : 2005 : Manila, Philippines).; Brar, D.S.; Mackill, D.; Hardy, B..
x, 355 p. : ill.
Tipo: Book Palavras-chave: Genetics; Genomes; Gene expression; Genetic regulation; Functional genomics; Ontogeny.
Ano: 2005 URL: http://hdl.handle.net/123456789/782
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T-DNA insertional mutagenesis in Arabidopsis: a tool for functional genomics Electron. J. Biotechnol.
Radhamony,Resmi Nath; Mohan Prasad,Anand; Srinivasan,Ramamurthy.
With the availability of complete genome sequences of several organisms, the focus has shifted from structural genomics to functional genomics, specifically in plants where the complete genomic sequences are becoming available i.e., Arabidopsis and rice. Agrobacterium mediated transformation which is exploited for transgenic technology is also being used as an effective mutagen and as a tool for functional genomics in higher plants. Besides the fact that the insertion of T-DNA element into a gene can lead to loss or gain of function, ingenious use of a variety of vectors have led to the identification of genes and regulatory elements in Arabidopsis. In this review, we highlight the progress made in the field of functional genomics of Arabidopsis using...
Tipo: Journal article Palavras-chave: Arabidopsis; Functional genomics; Insertional mutagenesis; Promoter trapping; T-DNA tagging.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100012
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Transcriptome profiling of rice seedlings under cold stress. Repositório Alice
MAIA, L. C. da.; CADORE, P. R. B.; BENITEZ, L. C.; DANIELLOWSKI, R.; BRAGA, E. J.; FAGUNDES, P. R. R.; MAGALHAES JUNIOR, A. M. de; OLIVEIRA, A. C. de..
2017
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Abiotic stress; Chilling; Expression; Functional genomics; Transcripts.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1068357
Registros recuperados: 9
Primeira ... 1 ... Última
 

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