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Overproduction of proline in transgenic hybrid larch (Larix x leptoeuropaea (Dengler) cultures renders them tolerant to cold, salt and frost Inra
Gleeson, D.; Lelu-Walter, M.A.; Parkinson, M..
Cold, salt and frost are important environmental stresses in forest trees and may significantly reduce productivity. Elevated levels of proline are associated with these stresses and may help alleviate their effects. Transgenic hybrid larch Larix leptoeuropaea has been produced expressing a Vigna aconitifolia gene for pyrroline 5-carboxylate synthase, the rate-limiting step in proline synthesis. Embryogenic masses of hybrid larch were co-cultivated with Agrobacterium tumefaciens harbouring a binary vector expressing the gene. The integration of the gene into the plant genome was confirmed by Southern blot and by proline content analysis. There was an approximately 30-fold increase in proline level in transgenic tissue compared to non-transformed controls....
Tipo: Journal Article Palavras-chave: MELEZE HYBRIDE; TRANSGENIQUE; EXPRESSION DES GENES; PROLINE; TOLERANCE AU STRESS; FROID; SEL; GEL LARIX LEPTOEUROPAE.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20073c8098df&uri=/notices/prodinra1/2007/04/
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