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Contribution à l'étude de l'influence de la température sur la gamétogenèse de l'huître perlière Pinctada margaritifera (Linné, 1758) en conditionnement ArchiMer
Viard, Carole.
L'industrie de la perle nmre étant florissante en Polynésie française, l'aquaculture de l'huître perlière Pinctada margaritifera (Linné, 1758) suscite de nombreuses recherches. L'objectif de cette étude était d'observer le contrôle qu'exerce la température sur la gamétogenèse de la nacre et de voir s'il était possible de synchroniser, grâce à ce paramètre, la maturité des individus en laboratoire. Les variations de températures annuelles observées dans le milieu naturel ont été reproduites sur huit semaines. Régulièrement, des lots de nacres ont été sacrifiés, plusieurs variables biométriques ont été mesurées et l'analyse histologique des gonades a permis d'identifier le stade de maturité. Les résultats ont montré que la température a eu une influence sur...
Tipo: Text Palavras-chave: Huîtres perlières; Pinctada margaritifera; Température; Gamétogenèse; Conditionnement.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00446/55760/57421.pdf
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Déterminismes génétiques de l'allocation à la reproduction chez les huîtres creuses (Crassostrea gigas) triploïdes ArchiMer
Normand, Julien.
In diploid Pacific oyster Crassostrea gigas, gonadic development can be important in terms of energetic investment. Phenotypic and genetic negative correlations have also been observed between gonadic occupation and traits related to survival and growth, and this fact suggests that this trait could be deleterious for its aquaculture production. Triploidy can be been artificially induced to improve the production of Pacific oysters as it results in a general diminution of mean gonadic development. This trait is however highly variable. In this context, this study aimed to quantify and qualify the allocation to reproduction in triploid oysters and to study its genetic bases. Variability for allocation to reproduction and growth was first studied in 3 sets of...
Tipo: Text Palavras-chave: Triploidy x genetic interaction; Genetic determinism; Sexual dimorphism; Gametogenesis; Allocation to reproduction; Triploid; Oyster; Interaction triploïdie x génétique; Déterminisme génétique; Dimorphisme sexuel; Gamétogenèse; Allocation à la reproduction; Triploïde; Huître.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/these-6971.PDF
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Ecophysiologie de l'huître creuse Crassostrea gigas (Thunberg) : analyse bioénergétique de la gamétogenèse sous trois conditions trophiques distinctes ArchiMer
Bourles, Yves.
The Pacific oyster (Crassostrea gigas) is a major cultivated shellfish resource in France, which represented a tumover of 220 millions € in 2000. Mass mortalities among this commercially important bivalve species are known in France since about 40 years, but have become a recun'ing obstacle for growers of oysters and a threaten for commercial production along the French Atlantic coast in the last 20 years. Since 2000, IFREMER is canying out a multidisciplinary program, MOREST, ta understand the problem of summer mortality, and possibly ta solve il. As part of the MOREST program, the present study focuses on the relationship between gametogenesis, metabo lism and mortality of 7-month, i.e. juvenil e, C. gigas. In April 2004, a total of 2250 oysters were...
Tipo: Text Palavras-chave: Crassostrea gigas; Naissain; Mortalité estivale; Gamétogenèse; Bilan énergétique; Crassostrea gigas; Spot; Summer mortalities; Gametogenesis; Energy budget.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/00448/55961/57444.pdf
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Etude cytologique de la gamétogenèse, de la sex-ratio et du cycle de reproduction chez l'huître perlière Pinctada margaritifera (L) var. cumingii (Jameson), (mollusques, bivalves). Comparaison avec le cycle de Pinctada maculata (Gould) ArchiMer
Thielley, Maryse.
The population of Pinctada margaritifera and Pinctada maculata lay eggs throughout the year. Temperature variations induce huge but asynchronous laying. Preferential periods of gonad maturity correspond to the season changings in both species. However. some differences between the two specics are noticed. Both species are protandric hermaphrodites. Female condition is unstable: a reversion to the male condition may be observed and is probably linked with stress. The average size for the first sexual maturation as weil as for sexual conversion are specified in P. margaritifera. The breeding conditions induce modifications in some reproductive cycle processes (maturation, sex-ratio). Some conditions suitable for reproduction may become unfavorable to...
Tipo: Text Palavras-chave: Gamétogenèse; Cycle de reproduction; Sex-ratio; Pinctad margaritifera; Mollusque; Bivalve; Huître perlière; Gametogenesis; Reproductive cycle; Sex-ratio; Pinctada margaritifera; Mollusca; Bivalvia; Pearl oyster.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00440/55208/56677.pdf
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Etude transcriptomique, génétique et fonctionnelle de la gamétogenèse chez l'huître creuse Crassostrea gigas ArchiMer
Bigot, L.; Dubos, M. P.; Dheilly, N.; Favrel, P.; Kellner, K.; Lelong, C.; Martinez, A. S.; Riviere, G.; Santerre, C.; Sourdaine, P.; Degremont, Lionel; Flahauw, Emilie; Hatt, Philippe-jacques; Heurtebise, Serge; Lapegue, Sylvie; Azzouzi, N.; Galibert, F.; Boudry, Pierre; Corporeau, Charlotte; Fabioux, Caroline; Guevelou, Eric; Huvet, Arnaud; Sussarellu, Rossana.
Le projet GAMETOGENES vise à améliorer les connaissances des bases physiologiques et génétiques de la reproduction et des métabolismes associés chez un mollusque d'importance économique l'huître creuse, Crassostrea gigas. Il a pour objectifs (1) la définition de marqueurs clés de la gamétogenèse, de l'investissement reproducteur et du déterminisme du sexe chez une espèce hermaphrodite à protandrie irrégulière (2) la recherche de QTL de survie aux mortalités estivales ou à l'effort de reproduction dont le succès repose sur l'augmentation de la densité de marqueurs sur les cartes génétiques existantes (SNP en particulier) et sur les liens qui pourront être établis entre ces cartes de liaison et une carte physique (3) le développement d'outils pour valider la...
Tipo: Text Palavras-chave: Génétique; Huître creuse; Crassostrea gigas; Gamétogenèse; QTL; Marqueur; Physiologie.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00107/21781/19355.pdf
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Gametogenic cycle and reproductive effort of the tropical blacklip pearl oyster, Pinctada margaritifera (Bivalvia : Pteriidae), cultivated in Takapoto atoll (French Polynesia) ArchiMer
Pouvreau, Stephane; Gangnery, Aline; Tiapari, Jerome; Lagarde, Franck; Garnier, Matthieu; Bodoy, Alain.
The gametogenic cycle and the reproductive effort of the blacklip pearl oyster, Pinctada margaritifera, cultivated in Takapoto lagoon were studied for a 1-year period (March 1997-April 1998) by bimonthly observations of gonadal sections, dry tissue weights and gonadal index in a population of pearl oyster composed of three age-groups. pearl oysters attained sexual maturity in the end of their first year (height approximate to 40 mm), implying that P. margaritifera is a late-maturing species in comparison with other Pteriidae. This species was also confirmed to be a marked protandrous successive hermaphrodite in culture, with 100 % of males at first maturity and 75 % in older pearl oyster (height > 120 mm). The general pattern of gametogenic activity,...
Tipo: Text Palavras-chave: French Polynesia; Reproductive effort; Reproductive cycle; Tropical aquaculture; Gametogenesis; Blacklip pearl oyster; Polynésie française; Effort de reproduction; Cycle de reproduction; Aquaculture tropicale; Gamétogenèse; Huître perlière.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-1391.pdf
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Le déterminisme du sexe de l’huître creuse Crassostrea gigas au cours de son développement et du cycle gamétogénétique adulte : recherche de déterminants moléculaires ArchiMer
Naimi, Amine.
The pacific oyster Crassostrea gigas is a successive protandric irregular hermaphrodite. The factors involved in the first gonadic differentiation in juveniles and those involved in sex reversal in adults are still unknown. This thesis consisted in the identification of molecular factors of the oyster’s gonadic differentiation during its adult cycle and its development. Thus, the respective orthologs to a DM-family factor and to Foxl2, Cg-DMl and Cg-Foxl2, have been characterized in the oyster by RT-PCR. Their study by real-time PCR, in situ hybridization and immunohistochemistry, has highlighted a ubiquitous expression of Cg-DMl in potentially proliferating / differentiating cells in the gonads of both sexes. The Cg-Foxl2 expression is specific to the...
Tipo: Text Palavras-chave: Différenciation sexuelle; Crassostrea gigas; Mollusques marins; Gamétogenèse; Hermaphrodisme; DMRT; FOXL2; Sex differentiation; Crassostrea gigas; Marin molluscs; Gametogenesis; Hermaphroditism; DMRT; FOXL2.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00012/12291/9080.pdf
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Origine et développement des cellules germinales chez l'huître creuse Crassostrea gigas : Intérêt pour le contrôle de la reproduction en écloserie. ArchiMer
Fabioux, Caroline.
A comprehensive knowledge of Crassostrea gigas oyster reproduction processes is essential to optimize controlled reproduction in hatchery. This study aimed to determine the origin and the mechanisms of annual renewal of germ cells, still unknown in oysters, and the influence of temperature and photoperiod parameters on the regulation of the reproductive cycle. We characterized an orthologue of the vasa gene, the Oyvlg (Oyster vasa-like gene), the first germ cell specific marker isolated in molluscs. This marker was used to study C. gigas germline development. Oyster germ cells appeared specified during early development from mesentoblast 4d, as primordial germ cells (PGCs) determined by maternal cytoplasmic determinants, including Oyvlg mRNA. In adult...
Tipo: Text Palavras-chave: Controlled reproduction; Regulation; Oyster vasa like gene; Gametogenesis; Germ cells; Crassostrea; Reproduction contrôlée; Régulation; Oyster vasa like gene; Gamétogenèse; Cellules germinales; Crassostrea.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/these-1380.pdf
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