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A first generation genetic linkage map of the European flat oyster Ostrea edulis (L.) based on AFLP and microsatellite markers ArchiMer
Lallias, Delphine; Beaumont, A.r.; Haley, C.s.; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
This study presents the first genetic linkage map for the European flat oyster Ostrea edulis. AFLP and 20 microsatellite markers were genotyped in a three-generation pedigree comprised of 2 grand-parents, 2 parents and 92 progeny. Chi-square goodness of fit tests revealed high segregation distortion, which was significant for 32.8% of markers. Sixteen microsatellites and 235 AFLPs (170 AFLPs type 1:1 and 65 AFLPs type 3:1) were used to build sex-specific linkage maps using CriMap software. The first parental map (P1) consisted of 104 markers grouped in nine linkage groups, spanning 471.2 cM with an average spacing of 4.86 cM. The second parental map (P2) consisted of 117 markers grouped in ten linkage groups (which equals the haploid chromosome number),...
Tipo: Text Palavras-chave: Microsatellite; AFLP; Ostrea edulis; Flat oyster; Genetic linkage map.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2642.pdf
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A first genetic linkage map of the blue mussel Mytilus edulis ArchiMer
Lallias, Delphine; Hecquet, Céline; Boudry, Pierre; Lapegue, Sylvie; Beaumont, Andy.
The blue mussel, Mytilus edulis, is widely distributed in the northern hemisphere, from the White Sea to the Atlantic coast of southern France, and is an economically important species (1 million tons per year, FAO, 2002). At present however, little domestication has been achieved with bivalves, in contrast to agricultural crops, livestock and horticultural species like tomatoes, where selection and production is entirely controlled by man. For bivalves, despite their economic importance, no lines with selected characteristics have been taken to the production scale. There is therefore a need to make up for lost time. Our interest in genetic mapping sprang from some results showing a genetic component to some economically important traits like growth and...
Tipo: Text Palavras-chave: QTLs; AFLP; Genetic; Mussel; Mytilus edulis; Genetic linkage map.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3465.pdf
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A first genetic linkage map of the European flat oyster, Ostrea edulis : towards the identification of Bonamia resistance QTLs ArchiMer
Lallias, Delphine; Lapegue, Sylvie; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Beaumont, Andy.
The flat oyster Ostrea edulis is an endemic European oyster species, found on both Atlantic and Mediterranean coasts. Its aquacultural production has dramatically decreased due to two successive diseases caused by the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has undertaken a breeding program to produce oyster families which are tolerant to Bonamia. In this context, the next step is to identify QTLs for resistance to this parasite. A genetic map is therefore being established as a basis for QTL work. The reference family is an F2 family produced by single pair mating of two oysters from the same F1 family. The F1 family was itself produced from a bi-parental cross between a wild oyster and an individual from the...
Tipo: Text Palavras-chave: Genetic linkage map; Parasite; Genetic; QTLs; Resistance; Bonamia ostreae; Ostrea edulis; Flat oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3464.pdf
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A preliminary genetic linkage map for the European flat oyster Ostrea eduli ArchiMer
Lallias, Delphine; Lapegue, Sylvie; Halley, Chris; Boudry, Pierre; Beaumont, Andy.
The European flat oyster, Ostrea edulis, occurs naturally from Norway to Morocco in the North-Eastern Atlantic and throughout the Mediterranean. The oyster has been a harvested species for at least 6000 years. However, it successively suffered from over harvesting and then from two protozoan diseases during the 1960s and its production has drastically decreased in Europe from that time. Following two generations of selection for tolerance to one of these diseases (bonamiosis), our aim is to optimize this selection with the assistance of molecular markers, that is to say to implement marker-assisted selection. This will enhance the efficiency of selection by reducing the generation time, as the selection on sites cannot be made before the oysters are...
Tipo: Text Palavras-chave: QTLs; Ostrea edulis; Genetic linkage map; Genetic; European flat oyster.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3473.pdf
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A preliminary genetic linkage map for the European flat oyster Ostrea edulis L. ArchiMer
Lallias, Delphine; Lapegue, Sylvie; Harley, Crhis; Boudry, Pierre; Heurtebise, Serge; Beaumont, Andy.
The flat oyster Ostrea edulis is the species endemic to European coasts, both Atlantic and Mediterranean. It has been exploited since Roman times, but its aquaculture production decreased from around 20000 tons in the 1950's to 1500 tons today because of two successive diseases due to the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has been undertaking a breeding program to produce families of oysters tolerant to Bonamia. In this context, a further step would be to identify QTLs for resistance to this parasite. Therefore, as a first step a genetic map is now being built. The establishment of a genetic linkage map will provide a foundation for the mapping of QTLs, with the ultimate objective to implement...
Tipo: Text Palavras-chave: QTLs; Ostrea edulis; Flat oyster; Genetic linkage map; Genetic.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3466.pdf
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AFLP-based genetic linkage maps of the blue mussel (Mytilus edulis) ArchiMer
Lallias, Delphine; Lapegue, Sylvie; Hecquet, Celine; Boudry, Pierre; Beaumont, A.
We report the construction of the first genetic linkage map in the blue mussel, Mytilus edulis. AFLP markers were used in 86 full-sib progeny from a controlled pair mating, applying a double pseudo-test cross strategy. Thirty-six primer pairs generated 2354 peaks, of which 791 (33.6%) were polymorphic in the mapping family. Among those, 341 segregated through the female parent, 296 through the male parent (type 1:1) and 154 through both parents (type 3:1). Chi-square goodness-of-fit tests revealed that 71% and 73% of type 1:1 and 3:1 markers respectively segregated according to Mendelian inheritance. Sex-specific linkage maps were built with mapmaker 3.0 software. The female framework map consisted of 121 markers ordered into 14 linkage groups, spanning...
Tipo: Text Palavras-chave: Mytilus edulis; Genetic linkage map; Blue mussel; AFLP.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2830.pdf
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Detection of quantitative trait loci affecting short lateral branching in Cucumis melo National Institute of Agronomic Research
Fukino, N.; Sugiyama, M.; Ohara, T.; Sainoki, H.; Kubo, N.; Hirai, M.; Matsumoto, S.; Sakata, Y..
Labor-saving techniques for pruning lateral branches are desirable in Japanese melon production. A short-lateral-branching line, Nou4 was developed from a weed-type ‘LB-1’ (Cucumis melo var. agrestis). In this study, quantitative trait loci(QTL) analysis for short lateral branching was conducted with a population of 94 F2 derived from a cross between Nou4 and normal-branching cultivar ‘Earl’s Favourite (Harukei 3)’. A total of 114 markers including 94 SSRs, 2 CAPS, and 18 AFLPs were employed to construct a linkage map with 13 linkage groups. Two QTLs controlling short lateral branching were identified on LG VII and LG XI. The QTL on LG VII explained 14.8 % of the phenotypic variance with a LOD score of 3.2. The QTL on LG XI explained 42.2 % of the...
Tipo: Poster Palavras-chave: Melon; Lateral shoot; Labor-saving; Genetic linkage map; QTL analysis; Phenotypic variance; Cucumis melo; QTL.
Ano: 2008 URL: http://hdl.handle.net/2174/278
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Detection of two QTLs for fruit weight in cucumber (Cucumis sativus) National Institute of Agronomic Research
Heang, D.; Sato, H.; Sassa, H.; Koba, T..
Cucumber has variations in traits of such as fruits with different weights, shapes, spine colors, etc. These traits are controlled by either multiple or single genes. An F2 population with molecular markers such as AFLP and SSR markers was used to develop a genetic linkage map of cucumber. The two parents have a large difference in traits which led us able to detect two QTLs, fw1 and fw2, for fruit weight. Furthermore, a gene for spine morphology was also mapped on the linkage group 7. These results could be useful for comparative genetic studies as well as marker assisted selection.
Tipo: Poster Palavras-chave: AFLP marker; SSR marker; Genetic linkage map; Fruit weight.
Ano: 2008 URL: http://hdl.handle.net/2174/255
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Integrated consensus map of cultivated peanut and wild relatives reveals structures of the A and B genomes of Arachis and divergence of the legume genomes. Repositório Alice
SHIRASAWA, K.; BERTIOLI, D. J.; VARSHNEY, R. K.; MORETZSOHN, M. de C.; BERTIOLI, S. C. de M. L.; THUDI, M.; PANDEY, M. K.; RAMI, J. F.; FONCÉKA, D.; GOWDA, M. V. C.; QIN, H.; GUO, B.; YANBIN, H.; XUANQIANG, L.; HIRAKAWA, H.; TABATA, S.; ISOBE, S..
bitstream/item/179844/1/dss042.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Arachis spp; Comparative genomics; Genetic linkage map; Integrated consensus map; Legume genome.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/963730
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LINKAGE MAPPING OF CANDIDATE GENES FOR INDUCE RESISTANCE AND GROWTH PROMOTION BY Trichoderma koningiopsis (Th003) IN TOMATO Solanum lycopersicum Acta biol.Colomb.
SIMBAQUEBA,JAIME; COTES,ALBA MARINA; BARRERO,LUZ STELLA.
Induced systemic resistance (ISR) is a mechanism by which plants enhance defenses against any stress condition. ISR and growth promotion are enhanced when tomato (Solanum lycopersicum) is inoculated with several strains of Trichoderma ssp. This study aims to genetically map tomato candidate genes involved in ISR and growth promotion induced by the Colombian native isolate Trichoderma koningiopsis Th003. Forty-nine candidate genes previously identified on tomato plants treated with Th003 and T. hamatum T382 strains were evaluated for polymorphisms and 16 of them were integrated on the highly saturated genetic linkage map named “TOMATO EXPEN 2000”. The location of six unigenes was similar to the location of resistance gene analogs (RGAs),...
Tipo: Journal article Palavras-chave: Trichoderma koningiopsis; Solanum lycopersicum; Induced systemic resistance growth promotion; Candidate genes; Genetic linkage map.
Ano: 2011 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2011000200004
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