SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Coeficiente de trilha no estudo dos componentes primarios e secundarios na producao de graos do guandu (Cajanus cajan (L.) Millsp).

QUEIROZ, I. dos S.; DIAS, N. dos S.; MIRANDA, C. M.; BARBOSA, A. C. O.; SANTOS, W. N.; PESTANA, K. N.; SILVA, P. H.; VELAME,L K. V. C.; HADDAD, F.; AMORIM, E. P.; FERREIRA, C. F..

A banana (Musa spp.) é uma das frutas mais consumidas no país, possui alto valor nutritivo e encontra-se disponível durante todo ano. Contudo, a bananeira ainda é acometida por vários fatores bióticos e abióticos.

Tipo: Resumo em anais de congresso (ALICE)

Palavras-chave: Bnanana; Fusarium oxysporum cubense; Mal do Panamá; Marcador mlecular; Marcado genético; Doença de planta; Genetic marker; Plant diseases; Disorders.

Ano: 2014

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1004988

SANTOS, C. A. F.; MENEZES, E. A.; PAINI, J. N.; CRUZ, C. D..

Cincoenta e seis acessos de guandu foram usados para estudar os efeitos diretos e indiretos dos componentes primarios e secundarios sobre a producao de graos. O estudo do coeficiente de trilha, adotando um diagrama em cadeias, revelou que os componentes primarios, numero de vagens/planta e tamanho dos graos apresentaram maiores efeitos diretos associados a alta correlacao. O numero de vagens/planta foi influenciado por plantas de maturacao precoce altas, enquanto o tamanho de graos foi influenciado pelo comprimento de vagens e pela altura da planta. Recomenda-se na selecao de guandu granifero considerar plantas com maior numero de vagens e sementes maiores como componentes primarios e plantas altas, de vagens compridas, floracao tardia e maturacao precoce...

Desenvolvimento de SNP (Single-Nucleotide Polymorphism) para estudos de variação genética e estabelecimento de coleção nuclear em mamoeiro (Carica papayaL.).

Palavras-chave: Pigeonpea; Genetic marker; Guandu; Cajanus Cajan; Grão; Marcador Genético; Produção; Grain yield.

Ano: 1994

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/133144

OLIVEIRA, G. A. F.; DANTAS, J. L. L.; OLIVEIRA, E. J. de.

Marcadores moleculares têm sido utilizados em diversos estudos associados ao manejo, conservação e uso de recursos genéticos em diversas espécies.

Tipo: Resumo em anais de congresso (ALICE)

Palavras-chave: Mamão; Carica Papaya; Marcador molecular; Single nucleotide polymorphism; Papayas; Genetic marker.

Ano: 2014

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1005008

Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification

Neotropical Ichthyology

Souza-Shibatta,Lenice; Ferreira,Dhiego Gomes; Oliveira,Claudio; Almeida,Fernanda Simões de; Shibatta,Oscar Akio; Sofia,Silvia Helena.

Thirteen microsatellite loci were isolated and characterized in Microglanis cottoides. Of these, two were monomorphic and 11 were polymorphic. These polymorphic loci tested on 24 individuals from a wild population produced a total of 108 different alleles, with levels of variability high, ranging from 2 to 20, with an average of 8.3 alleles per locus. The observed and expected heterozygosity ranged from 0.125 to 0.958 and from 0.119 to 0.931, respectively. A high combined probability of paternity exclusion value and a low probability combined genetic identity value obtained show that the set of loci described herein displays good suitability for paternity studies and differentiation of M. cottoides. Additionally, all thirteen microsatellite primers...

Tipo: Info:eu-repo/semantics/article

Palavras-chave: Brazilian Eastern basin; Catfish; Enriched genomic library; Genetic marker; SSR.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300581

Development of mussel-specific expression vectors suitable for transgenic organisms: implication on the establishment of mollusc continuous cell lines

Delsert, Claude; Cancela Da Fonseca, Leonor.

The main objective of this project was to develop the tools for the in-depth understanding of mollusc biology and pathology. Such a goal has been reached in other biological systems through the development of molecular biology and cell culture. Indeed, development of a mollusc cell culture system is an essential step to approach many of the problems related to mollusc pathology, in particular to use molecular biology tools to study viral infection of these organisms. No continous cell line is available from bivalves and while cell cultures have been obtained from these organisms they are difficult to develop and labor intensive, survival cultures being maintained only for a few weeks. Our goal has been to study in detail many of the parameters involved in...

Tipo: Text

Palavras-chave: Pathology; Mussel-specific expression vectors; Cell culture; Mytilus galloprovincialis; Gene; Genetic marker.

Ano: 1998

URL: http://archimer.ifremer.fr/doc/00044/15540/12917.pdf

Hibridacao natural em guandu no sertao pernambucano.

SANTOS, C.A.F.; MENEZES, E.A.; ARAUJO, F.P. de.

Avaliou-se a extensao da hibridacao natural do guandu em plantas individuais e em plantas de uma fileira, usando-se dois genotipos e duas epocas de cultivo, no municipio de Petrolina, PE. Foi usada a cor do talo como marcador genetico, devido a heranca simples e a dominancia completa da cor violeta sobre a cor verde. A hibridacao natural foi maior na segunda colheita nem relacao a primeira, tambem a hibridacao em plantas individuais foi superior a observada em plantas de uma fileira. Nao se observaram diferencas marcantes na hibridacao natural entre os dois genotipos, nem entre as avaliacoes efetuadas em diferentes epocas do ano. As taxas medias de hibridacao natural que foram observadas oscilaram de 2,50% a 13,36% nas plantas individuais, e de 0,86% a...

Hibridacao natural em guandu no Sertao pernambucano.

Palavras-chave: Cajanus cajan; Melhoramento genetico; Marcador genetico; Plant breeding; Genetic marker.

Ano: 1995

URL: http://www.alice.cnptia.embrapa.br/handle/doc/91396

SANTOS, C. A. F.; MENEZES, E. A.; ARAUJO, F. P. de.

Avaliou-se a extensao da hibridacao natural do guandu em plantas individuais e em plantas de uma fileira, usando-se dois genotipos e duas epocas de cultivo, no municipio de Petrolina, PE. Foi usada a cor do talo como marcador genetico, devido a heranca simples e a dominancia completa da cor violeta sobre a cor verda. A hibridacao natural foi maior na segunda colheita em relacao a primeira, tambem a hibridacao em plantas individuais foi superior a observada em plantas de um fileira. Nao se observaram diferencas marcantes na hibridacao natural entre os dois genotipos, nem entre as avaliacoes efetuadas em diferenes epocas do ano. As taxas medias de hibridacao natural que foram observadas oscilaram de 2,50% a 13,36% nas plantas individuais, e de 0,86% a 22,4%...

Importance and future of individual markers for the ecosystem approach to fisheries

Palavras-chave: Hibrido; Marcador genetico; Cajanus cajan; Plant breeding; Genetic marker; Guandu; Leguminosa; Melhoramento genetico.

Ano: 1995

URL: http://www.alice.cnptia.embrapa.br/handle/doc/133140

Fromentin, Jean-marc; Ernande, Bruno; Fablet, Ronan; De Pontual, Helene.

The use of genetic, biochemical and electronic markers in population biology and ecology has been growing tremendously during the last two decades. The first part of this paper aims at reviewing the main principles and advances of these individual markers through a few key applications on exploited marine fish populations. The second part is more prospective and investigates some possibilities that could arise in the near future through: (i) the development of new markers, (ii) the combination of different markers and (iii) the combination of quantitative approaches-whether classical or new-with individual markers. It is finally stressed how crucial individual markers will be to unravel the biocomplexity of wild fish populations and the key role they...

Tipo: Text

Palavras-chave: Genetic marker; Biochemical marker; Electronic tag; Otolith; Isotope; Population structure; Statistical and mechanistic model; Fisheries.

Ano: 2009

URL: http://archimer.ifremer.fr/doc/00000/11163/7522.pdf

Mode de reconnaissance hôte symbionte en milieux extrêmes : cas du modèle symbiotique Rimicaris exoculata

PCR survey of 50 introns in animals: Cross-amplification of homologous EPIC loci in eight non-bilaterian, protostome and deuterostome phyla

Le Bloa, Simon.

Deprived of light, the deep-sea hydrothermal vents are extremes ecosystems sustained by micobial chemosynthesis. These environments are colonized by animal species living in close relationships with these chemoautotrophic micro-organisms, eating them or establishing long term interactions with them, may they be trophic or not only. The shrimp Rimicaris exoculata is an endemic hydrothermal species of the Mid-Atlantic Ridge (MAR) sites. This crustacean represents the predominant macrofauna of some sites of the MAR. It lives in symbiotic association with two distinct microbial communities qualified as ectosymbiosis. One is located in its gill chamber and one in its gut. First, this work focused on the study of bacterial communication (Quorum Sensing or QS)...

Tipo: Text

Palavras-chave: Écosystèmes hydrothermaux; Peptide antimicrobien; Marqueurs génétiques; Hydrothermal vent; Rimicaris exoculata; Symbiose; Quorum Sensing; Antimicrobial peptide; Genetic marker.

Ano: 2016

URL: https://archimer.ifremer.fr/doc/00498/60918/64279.pdf

Polymorphism of the grasshopper Rhammatocerus schistocercoides populations revealed by RAPD.

Gerard, K.; Guilloton, E.; Arnaud-haond, Sophie; Aurelle, D.; Bastrop, R.; Chevaldonne, P.; Derycke, S.; Hanel, R.; Lapegue, Sylvie; Lejeusne, C.; Mousset, Sylvain; Ramsak, A.; Remerie, T.; Viard, Frederique; Feral, Jean-pierre; Chenuil, A..

Exon Primed Intron Crossing (EPIC) markers providemolecular tools that are susceptible to be variable within specieswhile remaining amplifiable by PCR using potentially universal primers. In this studywe tested the possibility of obtaining PCR products from 50 EPIC markers on 23 species belonging to seven different phyla (Porifera, Cnidaria, Arthropoda, Nematoda, Mollusca, Annelida, Echinodermata) using 70 new primer pairs. A previous study had identified and tested those loci in a dozen species, including another phylum, Urochordata (Chenuil et al., 2010). Results were contrasted among species. The best results were achieved with the oyster (Mollusca) where 28 loci provided amplicons susceptible to contain an intron according to their size. This was...

Tipo: Text

Palavras-chave: Universal primers; Alternative barcoding; Non-model species; Genetic marker; Intron.

Ano: 2013

URL: http://archimer.ifremer.fr/doc/00166/27775/25969.pdf

SILVA, J.B.T. da; TIGANO, M.S.; MAGALHÃES, B.P.; CORDEIRO, C.M.T..

The objective of this work was to study the genetic variability of the grasshopper Rhammatocerus schistocercoides (Orthoptera: Acrididae) using RAPD analysis among individuals from three populations, one from Colombia and two from Brazil (Goiás and Mato Grosso States). Ninety scorable binary markers were obtained by fingerprinting with 11 oligonucleotide primers. Most of the polymorphism was attributed to 42 markers with variable frequency among the different populations. Although the existence of significant difference among populations (P<0.0001), most of the genetic variability was found within populations (87.7% of total variation). Pairwise distances between Colombian and Brazilian populations were 0.12 (P<0.0001) and 0.18 (P<0.0001) for...

Polymorphism of the grasshopper Rhammatocerus schistocercoides populations revealed by RAPD

Palavras-chave: Insecta; Genetic marker; Genetic variation; Genetic distance; Marcador genético; Variação genética; Distância genética.

Ano: 2002

URL: http://www.alice.cnptia.embrapa.br/handle/doc/108815

PAB

Silva,João Batista Tavares da; Tigano,Myrian Silvana; Magalhães,Bonifácio Peixoto; Cordeiro,Célia Maria Torres.

Tipo: Info:eu-repo/semantics/article

Palavras-chave: Insecta; Genetic marker; Genetic variation; Genetic distance.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2002001100020

Target selected treatment with levamisole to control the development of anthelmintic resistance in a sheep flock.

CHAGAS, A. C. de S.; DOMINGUES, L. F.; GAINZA, Y. A.; BARIONI JUNIOR, W.; ESTEVES, S. N.; NICIURA, S. C. M..

Levamisole phosphate, chosen based on its 100 % efficacy demonstrated by a previous fecal egg count reduction test (FECRT), was used as the exclusive anthelmintic treatment in the Embrapa Southeast Livestock sheep flock from 2009 to 2014 in a target selected treatment scheme. In the present study, the effectiveness of this nematode control scheme was evaluated after 5 years by FECRT, larval development test (LDT), and a molecular test to assess the development of levamisole resistance in Haemonchus contortus. Animals were submitted to treatments with albendazole, levamisole, closantel, ivermectin, moxidectin, and monepantel. Eggs per gram of feces (EPG) counts and fecal cultures were performed, and anthelmintic efficacy was calculated by the RESO 4.0...

Utilização de PCR multiplex a partir de marcadores VNTR em mandioca.

Palavras-chave: Haemonchuscontortus isolates FECRT; Genetic marker; Acr8 gene; Levamisole resistance; Reversion.

Ano: 2015

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1030316