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Registros recuperados: 17
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Analysis of restriction fragment length polymorphism in the kappa-casein gene related to weight expected progeny difference in Nellore cattle Genet. Mol. Biol.
Biase,Fernando H.; Garnero,Analia Del V.; Bezerra,Luiz A.F.; Rosa,Arthur J.M.; Lôbo,Raysildo B.; Martelli,Lúcia.
Restriction fragment length polymorphism (RFLP) has been detected at the bovine kappa-casein locus. The polymorphism has been analyzed for its effects in cattle production, mostly for milk traits and even for maternal effect on pre-weaning weights. We used polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) to genotype 408 Nellore animals for the non-silent mutation (Thr/Ile136 and Asp/Ala148) that characterizes the A and B variants of the polymorphism and compared expected progeny difference (EPD) for a maternal effect on 120 and 210 days weights and direct EPD for 120, 210, 450 and 550 day weight between AA and AB animals. The EPD values were obtained from the University of São Paulo (Brazil) Nellore Cattle Breeding Program,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Weight; Kappa-casein; Genetic marker.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100014
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Bulks de DNA para identificação de marcadores SCAR relacionados à resistência a Fusarium oxysporum cubense. Repositório Alice
QUEIROZ, I. dos S.; DIAS, N. dos S.; MIRANDA, C. M.; BARBOSA, A. C. O.; SANTOS, W. N.; PESTANA, K. N.; SILVA, P. H.; VELAME,L K. V. C.; HADDAD, F.; AMORIM, E. P.; FERREIRA, C. F..
A banana (Musa spp.) é uma das frutas mais consumidas no país, possui alto valor nutritivo e encontra-se disponível durante todo ano. Contudo, a bananeira ainda é acometida por vários fatores bióticos e abióticos.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Bnanana; Fusarium oxysporum cubense; Mal do Panamá; Marcador mlecular; Marcado genético; Doença de planta; Genetic marker; Plant diseases; Disorders.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1004988
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Coeficiente de trilha no estudo dos componentes primarios e secundarios na producao de graos do guandu (Cajanus cajan (L.) Millsp). Repositório Alice
SANTOS, C. A. F.; MENEZES, E. A.; PAINI, J. N.; CRUZ, C. D..
Cincoenta e seis acessos de guandu foram usados para estudar os efeitos diretos e indiretos dos componentes primarios e secundarios sobre a producao de graos. O estudo do coeficiente de trilha, adotando um diagrama em cadeias, revelou que os componentes primarios, numero de vagens/planta e tamanho dos graos apresentaram maiores efeitos diretos associados a alta correlacao. O numero de vagens/planta foi influenciado por plantas de maturacao precoce altas, enquanto o tamanho de graos foi influenciado pelo comprimento de vagens e pela altura da planta. Recomenda-se na selecao de guandu granifero considerar plantas com maior numero de vagens e sementes maiores como componentes primarios e plantas altas, de vagens compridas, floracao tardia e maturacao precoce...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Pigeonpea; Genetic marker; Guandu; Cajanus Cajan; Grão; Marcador Genético; Produção; Grain yield.
Ano: 1994 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/133144
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Desenvolvimento de SNP (Single-Nucleotide Polymorphism) para estudos de variação genética e estabelecimento de coleção nuclear em mamoeiro (Carica papayaL.). Repositório Alice
OLIVEIRA, G. A. F.; DANTAS, J. L. L.; OLIVEIRA, E. J. de.
Marcadores moleculares têm sido utilizados em diversos estudos associados ao manejo, conservação e uso de recursos genéticos em diversas espécies.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Mamão; Carica Papaya; Marcador molecular; Single nucleotide polymorphism; Papayas; Genetic marker.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1005008
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Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification Neotropical Ichthyology
Souza-Shibatta,Lenice; Ferreira,Dhiego Gomes; Oliveira,Claudio; Almeida,Fernanda Simões de; Shibatta,Oscar Akio; Sofia,Silvia Helena.
Thirteen microsatellite loci were isolated and characterized in Microglanis cottoides. Of these, two were monomorphic and 11 were polymorphic. These polymorphic loci tested on 24 individuals from a wild population produced a total of 108 different alleles, with levels of variability high, ranging from 2 to 20, with an average of 8.3 alleles per locus. The observed and expected heterozygosity ranged from 0.125 to 0.958 and from 0.119 to 0.931, respectively. A high combined probability of paternity exclusion value and a low probability combined genetic identity value obtained show that the set of loci described herein displays good suitability for paternity studies and differentiation of M. cottoides. Additionally, all thirteen microsatellite primers...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazilian Eastern basin; Catfish; Enriched genomic library; Genetic marker; SSR.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300581
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Development of mussel-specific expression vectors suitable for transgenic organisms: implication on the establishment of mollusc continuous cell lines ArchiMer
Delsert, Claude; Cancela Da Fonseca, Leonor.
The main objective of this project was to develop the tools for the in-depth understanding of mollusc biology and pathology. Such a goal has been reached in other biological systems through the development of molecular biology and cell culture. Indeed, development of a mollusc cell culture system is an essential step to approach many of the problems related to mollusc pathology, in particular to use molecular biology tools to study viral infection of these organisms. No continous cell line is available from bivalves and while cell cultures have been obtained from these organisms they are difficult to develop and labor intensive, survival cultures being maintained only for a few weeks. Our goal has been to study in detail many of the parameters involved in...
Tipo: Text Palavras-chave: Pathology; Mussel-specific expression vectors; Cell culture; Mytilus galloprovincialis; Gene; Genetic marker.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00044/15540/12917.pdf
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Hibridacao natural em guandu no sertao pernambucano. Repositório Alice
SANTOS, C.A.F.; MENEZES, E.A.; ARAUJO, F.P. de.
Avaliou-se a extensao da hibridacao natural do guandu em plantas individuais e em plantas de uma fileira, usando-se dois genotipos e duas epocas de cultivo, no municipio de Petrolina, PE. Foi usada a cor do talo como marcador genetico, devido a heranca simples e a dominancia completa da cor violeta sobre a cor verde. A hibridacao natural foi maior na segunda colheita nem relacao a primeira, tambem a hibridacao em plantas individuais foi superior a observada em plantas de uma fileira. Nao se observaram diferencas marcantes na hibridacao natural entre os dois genotipos, nem entre as avaliacoes efetuadas em diferentes epocas do ano. As taxas medias de hibridacao natural que foram observadas oscilaram de 2,50% a 13,36% nas plantas individuais, e de 0,86% a...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Cajanus cajan; Melhoramento genetico; Marcador genetico; Plant breeding; Genetic marker.
Ano: 1995 URL: http://www.alice.cnptia.embrapa.br/handle/doc/91396
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Hibridacao natural em guandu no Sertao pernambucano. Repositório Alice
SANTOS, C. A. F.; MENEZES, E. A.; ARAUJO, F. P. de.
Avaliou-se a extensao da hibridacao natural do guandu em plantas individuais e em plantas de uma fileira, usando-se dois genotipos e duas epocas de cultivo, no municipio de Petrolina, PE. Foi usada a cor do talo como marcador genetico, devido a heranca simples e a dominancia completa da cor violeta sobre a cor verda. A hibridacao natural foi maior na segunda colheita em relacao a primeira, tambem a hibridacao em plantas individuais foi superior a observada em plantas de um fileira. Nao se observaram diferencas marcantes na hibridacao natural entre os dois genotipos, nem entre as avaliacoes efetuadas em diferenes epocas do ano. As taxas medias de hibridacao natural que foram observadas oscilaram de 2,50% a 13,36% nas plantas individuais, e de 0,86% a 22,4%...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Hibrido; Marcador genetico; Cajanus cajan; Plant breeding; Genetic marker; Guandu; Leguminosa; Melhoramento genetico.
Ano: 1995 URL: http://www.alice.cnptia.embrapa.br/handle/doc/133140
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Importance and future of individual markers for the ecosystem approach to fisheries ArchiMer
Fromentin, Jean-marc; Ernande, Bruno; Fablet, Ronan; De Pontual, Helene.
The use of genetic, biochemical and electronic markers in population biology and ecology has been growing tremendously during the last two decades. The first part of this paper aims at reviewing the main principles and advances of these individual markers through a few key applications on exploited marine fish populations. The second part is more prospective and investigates some possibilities that could arise in the near future through: (i) the development of new markers, (ii) the combination of different markers and (iii) the combination of quantitative approaches-whether classical or new-with individual markers. It is finally stressed how crucial individual markers will be to unravel the biocomplexity of wild fish populations and the key role they...
Tipo: Text Palavras-chave: Genetic marker; Biochemical marker; Electronic tag; Otolith; Isotope; Population structure; Statistical and mechanistic model; Fisheries.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00000/11163/7522.pdf
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Mode de reconnaissance hôte symbionte en milieux extrêmes : cas du modèle symbiotique Rimicaris exoculata ArchiMer
Le Bloa, Simon.
Deprived of light, the deep-sea hydrothermal vents are extremes ecosystems sustained by micobial chemosynthesis. These environments are colonized by animal species living in close relationships with these chemoautotrophic micro-organisms, eating them or establishing long term interactions with them, may they be trophic or not only. The shrimp Rimicaris exoculata is an endemic hydrothermal species of the Mid-Atlantic Ridge (MAR) sites. This crustacean represents the predominant macrofauna of some sites of the MAR. It lives in symbiotic association with two distinct microbial communities qualified as ectosymbiosis. One is located in its gill chamber and one in its gut. First, this work focused on the study of bacterial communication (Quorum Sensing or QS)...
Tipo: Text Palavras-chave: Écosystèmes hydrothermaux; Peptide antimicrobien; Marqueurs génétiques; Hydrothermal vent; Rimicaris exoculata; Symbiose; Quorum Sensing; Antimicrobial peptide; Genetic marker.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00498/60918/64279.pdf
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PCR survey of 50 introns in animals: Cross-amplification of homologous EPIC loci in eight non-bilaterian, protostome and deuterostome phyla ArchiMer
Gerard, K.; Guilloton, E.; Arnaud-haond, Sophie; Aurelle, D.; Bastrop, R.; Chevaldonne, P.; Derycke, S.; Hanel, R.; Lapegue, Sylvie; Lejeusne, C.; Mousset, Sylvain; Ramsak, A.; Remerie, T.; Viard, Frederique; Feral, Jean-pierre; Chenuil, A..
Exon Primed Intron Crossing (EPIC) markers providemolecular tools that are susceptible to be variable within specieswhile remaining amplifiable by PCR using potentially universal primers. In this studywe tested the possibility of obtaining PCR products from 50 EPIC markers on 23 species belonging to seven different phyla (Porifera, Cnidaria, Arthropoda, Nematoda, Mollusca, Annelida, Echinodermata) using 70 new primer pairs. A previous study had identified and tested those loci in a dozen species, including another phylum, Urochordata (Chenuil et al., 2010). Results were contrasted among species. The best results were achieved with the oyster (Mollusca) where 28 loci provided amplicons susceptible to contain an intron according to their size. This was...
Tipo: Text Palavras-chave: Universal primers; Alternative barcoding; Non-model species; Genetic marker; Intron.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00166/27775/25969.pdf
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Polymorphism of the grasshopper Rhammatocerus schistocercoides populations revealed by RAPD. Repositório Alice
SILVA, J.B.T. da; TIGANO, M.S.; MAGALHÃES, B.P.; CORDEIRO, C.M.T..
The objective of this work was to study the genetic variability of the grasshopper Rhammatocerus schistocercoides (Orthoptera: Acrididae) using RAPD analysis among individuals from three populations, one from Colombia and two from Brazil (Goiás and Mato Grosso States). Ninety scorable binary markers were obtained by fingerprinting with 11 oligonucleotide primers. Most of the polymorphism was attributed to 42 markers with variable frequency among the different populations. Although the existence of significant difference among populations (P<0.0001), most of the genetic variability was found within populations (87.7% of total variation). Pairwise distances between Colombian and Brazilian populations were 0.12 (P<0.0001) and 0.18 (P<0.0001) for...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Insecta; Genetic marker; Genetic variation; Genetic distance; Marcador genético; Variação genética; Distância genética.
Ano: 2002 URL: http://www.alice.cnptia.embrapa.br/handle/doc/108815
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Polymorphism of the grasshopper Rhammatocerus schistocercoides populations revealed by RAPD PAB
Silva,João Batista Tavares da; Tigano,Myrian Silvana; Magalhães,Bonifácio Peixoto; Cordeiro,Célia Maria Torres.
The objective of this work was to study the genetic variability of the grasshopper Rhammatocerus schistocercoides (Orthoptera: Acrididae) using RAPD analysis among individuals from three populations, one from Colombia and two from Brazil (Goiás and Mato Grosso States). Ninety scorable binary markers were obtained by fingerprinting with 11 oligonucleotide primers. Most of the polymorphism was attributed to 42 markers with variable frequency among the different populations. Although the existence of significant difference among populations (P<0.0001), most of the genetic variability was found within populations (87.7% of total variation). Pairwise distances between Colombian and Brazilian populations were 0.12 (P<0.0001) and 0.18 (P<0.0001) for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insecta; Genetic marker; Genetic variation; Genetic distance.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2002001100020
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Target selected treatment with levamisole to control the development of anthelmintic resistance in a sheep flock. Repositório Alice
CHAGAS, A. C. de S.; DOMINGUES, L. F.; GAINZA, Y. A.; BARIONI JUNIOR, W.; ESTEVES, S. N.; NICIURA, S. C. M..
Levamisole phosphate, chosen based on its 100 % efficacy demonstrated by a previous fecal egg count reduction test (FECRT), was used as the exclusive anthelmintic treatment in the Embrapa Southeast Livestock sheep flock from 2009 to 2014 in a target selected treatment scheme. In the present study, the effectiveness of this nematode control scheme was evaluated after 5 years by FECRT, larval development test (LDT), and a molecular test to assess the development of levamisole resistance in Haemonchus contortus. Animals were submitted to treatments with albendazole, levamisole, closantel, ivermectin, moxidectin, and monepantel. Eggs per gram of feces (EPG) counts and fecal cultures were performed, and anthelmintic efficacy was calculated by the RESO 4.0...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Haemonchuscontortus isolates FECRT; Genetic marker; Acr8 gene; Levamisole resistance; Reversion.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1030316
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Utilização de PCR multiplex a partir de marcadores VNTR em mandioca. Repositório Alice
DIAS, N. dos S.; MIRANDA, C. M.; QUEIROZ, I. dos S.; BARBOSA, A. C. O.; PESTANA, K. N.; SILVA, P. H.; SANTOS, R. M. F.; OLIVEIRA, E. J. de; SANTOS, V. da S.; FERREIRA, C. F..
O uso de marcadores moleculares do tipo VNTR (Variable Number of Tandem Repeats) no equipamento Fragment Analyzer ? Automated TE System da Advanced Analytical,Bocasiona custos elevados por unidade de dado a ser analisado.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Mandioca; Marcador molecular; Genetic marker.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1005004
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การหาเครื่องหมายพันธุกรรมที่เกี่ยวข้องกับความสมบูรณ์พันธุ์ในโคนมภายใต้สภาพร้อนชื้น: รายงานการวิจัยฉบับสมบูรณ์ Thai Agricultural
Monchai Duangjinda; Suporn Katawatin; Yupin Phasuk; Tawin Wongpalub.
Tipo: PhysicalObject Palavras-chave: Dairy cattle; Hot stress; Humid condition; Genetic marker; Animal breeding; Dna; Microsatellites; Allele; Heat tolerance; Fertility; โคนม; ภูมิอากาศ; ความร้อน; ความชื้น; ความเครียด; การทนความร้อน; การปรับปรุงพันธุ์สัตว์; เครื่องหมายพันธุกรรม; ดีเอ็นเอ; ความทนทานต่อความเครียด; ไมโครแซทเทิลไลท์; อัลลีล; ความสมบูรณ์พันธุ์; การผลิตน้ำนม.
Ano: 2009 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5717
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ความหลากหลายของยีนต้านทานไวรัส (Mx1) ในสุกร Thai Agricultural
Santi Pinthukas; Christoph Knorr; Ratchaneewan Kumphakam; Bertram Brenig; Kesinee Gatphayak.
Disease is a major problem in the swine production. The study of genes associated with disease resistance characteristics is used to select for innate immunity in swine. The Mx1 gene was found to have potent antiviral affect against some virus and can be hereditary. The study of Mx1 polymorphisms in among 3 pig breeds (Thai native pig breeds, Chinese pig breeds and European pig breeds) were found SNPs in exon 8 (c. 1022C>G; 1078A>C) that has allele frequencies of statistical significance between the breeds. (P<0.05) The two variant in exon 15 found silent point mutation (c. 2120G>T) และ 11-bp indels (c.2117_2128 indels CGGCGCCGGCT) that has allele frequencies of statistical significance in some breed (P<0.05)especially in German Landrace and...
Tipo: Collection Palavras-chave: Porcine; Gene; Mx1; Virus resistance; Porcine myxovirus resistance gene; Genetic marker; สุกร; ยีน; การต้านทานไวรัส; อัลลีล; ความหลากหลายทางพันธุกรรม; ตัวบ่งชี้ทางพันธุกรรม.
Ano: 2011 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5189
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