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Registros recuperados: 52
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Agrobacterium-mediated transformation and detection of GUS reporter gene activity in IAC sugarcane genotypes. Repositório Alice
BOER, A. P. M.; FESTUCCI, C. D.; OLIVEIRA, I. C.; LANDEL, M. G. A.; MOLINARI, H. B. C.; GOLDMAN, M. H. S.; CRESTE, S..
2011
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Genetic transformation; Sucarcane; Agrobacterium; GUS; BAR gene.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/908727
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Agrobacterium-mediated transformation of Citrus sinensis and Citrus limonia epicotyl segments Scientia Agricola
Almeida,Weliton Antonio Bastos de; Mourão Filho,Francisco de Assis Alves; Mendes,Beatriz Madalena Januzzi; Pavan,Alexandra; Rodriguez,Adriana Pinheiro Martinelli.
Genetic transformation allows the release of improved cultivars with desirable characteristics in a shorter period of time and therefore may be useful in citrus breeding programs. The objective of this research was to establish a protocol for genetic transformation of Valencia and Natal sweet oranges (Citrus sinensis L. Osbeck) and Rangpur lime (Citrus limonia L. Osbeck). Epicotyl segments of germinated in vitro plantlets (three weeks in darkness and two weeks in a 16-h photoperiod) were used as explants. These were co-cultivated with Agrobacterium tumefaciens strain EHA-105 and different experiments were done to evaluate the transformation efficiency: explants were co-cultivated with Agrobacterium for one, three or five days; explants were incubated with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic transformation; Organogenesis; Micrografting; Improvement.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000100005
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Agrobacterium-mediated transformation of Guignardia citricarpa: an efficient tool to gene transfer and random mutagenesis. Repositório Alice
RODRIGUES, M. B. C.; FAVARO, L. C. de L.; PALUU, A. P. de. S.; FERREIRA, A.; SEBASTIANES, F. de. S.; RODRIGUES, M. J. C.; SPOSITO, M. B.; ARAUJO, W. L. de.; PIZZIRANI-KLEINER, A. A..
Guignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control CBS. This work describes for the first time an efficient and successful methodology for genetic transformation of G. citricarpa mycelia, which generated transformants expressing the gene encoding for the gfp (green fluorescent protein) and also their interaction with citrus plant. Mycelia of G. citricarpa were transformed via Agrobacterium tumefaciens, which carried the plasmid pFAT-gfp, contains the...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Agrobacterium-mediated; Transformation; Citrus Black Spot; Genetic transformation; Guignardia citricarpa; Plant e pathogen interaction.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/970991
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Applications of genomics to rice breeding International Rice Research Institute
Mackill, D. J..
p.9-15
Palavras-chave: Breeding; Genome analysis; Gene mapping; DNA sequencing; Genetic markers; Genetic markers; Genetic transformation; Gene expression.
Ano: 2003 URL: http://hdl.handle.net/123456789/1307
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Avaliacao da capacidade de regeneracao in vitro em tomateiro industrial. Repositório Alice
FARI, M.; RESENDE, G. M. de; MELO, N. F. de.
Este trabalho teve por objetivo avaliar a capacidade de regeneracao das cultivares de tomateiro industrial (Lycopersicon esculentum Mill) IPA-5 e IPA-6, utilizando quatro composicoes de meio de cultura descritos na literatura e cinco variacoes de inoculacao. Foi testada uma nova variacao de inoculacao, denominada cotiledone fendido. A maior frequencia de formacao de gemas vegetativas foi 100% no caso de IPA-5, e 65% no caso de IPA-6. Para induzir o alongamento de brotos, foram necessarios tres subcultivos dos explantes apresentando gemas. No caso de IPA-5, o numero de brotos obtidos foi maior quando a inducao de gemas foi realizada em meio contendo BAP e AIA seguido de tres subcultivos, em meio como zeatina. Usando esse protocolo, a cultivar IPA-5 produziu...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Tomate; Variedade; Organogenese; Cultura de tecido; Explante; Cotiledone; Transformacao genetica; Lycopersicon esculentum; Tomatoes; Varieties; Organogenesis; Tissue culture; Cotyledons; Explants; Genetic transformation.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/134062
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Biofortification: a global challenge program International Rice Research Institute
Graham, R. D..
p.4-8
Palavras-chave: Fortification; Research; Breeding; Genome analysis; Genetic transformation.
Ano: 2003 URL: http://hdl.handle.net/123456789/1306
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Biological activity of lectins from grain legumes and garlic against the legume pod borer, Helicoverpa armigera Open Agri
Arora, R.,( Richa Arora ).
Palavras-chave: Garlic; Pigeonpeas; Proteins; Transgenics; Transgenic plants; Chickpeas; Genes; Enzymes; Animal developmental stages; Genetic transformation.
Ano: 2005 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3230
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Changes in peroxidase and polypeptide profiles in Nicotiana tabacum L. transformed with Agrobacterium rhizogenes Ciência Rural
Echeverrigaray,Sergio.
Morphological and proteic modifications in Nicotiana tabacum L. transformed by Agrobacterium rhizogenes were evaluated by the comparison of normal and transformant plants regenerated from hairy-roots formed by the strains A4 or IB-642 of A. rhizogenes. Changes in apical dominance were observed in IB-642 transformants, which exhibited an abnormal development of axiliary buds. The electrophoretic analysis indicated an increase in peroxidase activity and the induction of several isozymes of this complex in the transformants. The SDS-PAGE patterns comparison allows to identify several changes, specially, the increase in 31-33 and 54 kD polypeptides in the transformants.. Biochemical analysis suggests the induction of a pathogen or stress like response of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic transformation; Agrobacterium rhizogenes; Nicotiana tabacum; Electrophoretic analysis.
Ano: 1995 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781995000200009
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Coffee biotechnology Braz. J. Plant Physiol.
De Los Santos-Briones,César; Hernández-Sotomayor,S. M. Teresa.
In the last three decades, interest has turned to in vitro cell culture in different areas of coffee research. In vitro techniques have been applied not only for coffee improvement through genetic transformation but also to study various aspects in coffee cells such as chemical (caffeine synthesis and the production of coffee aroma), physiological and more recently, biochemical aspects. The most important advances obtained to date on in vitro coffee techniques in fields like biochemistry, physiology, regeneration systems and genetic engineering, are presented and discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea; Coffee; Genetic transformation; In vitro tissue culture; Regeneration; Somatic embryogenesis.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100015
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Differential fine-tuning of gene expression regulation in coffee leaves by CcDREB1D promoter haplotypes under water deficit. Repositório Alice
ALVES, G. S. C.; TORRES, L. F.; DÉCHAMP, E.; BREITLER, J-C.; JOËT, T.; GATINEAU, F.; ANDRADE, A. C.; BERTRAND, B.; MARRACCINI, P.; ETIENNE, H..
Despite the importance of the DREB1D gene (also known as CBF4) in plant responses to water deficit and cold stress, studies analysing its regulation by transgenic approaches are lacking. In the current work, a functional study of three CcDREB1D promoter haplotypes (named HP15, HP16 and HP17) isolated from drought-tolerant and droughtsensitive clones of Coffea canephora was carried out in plants of C. arabica stably transformed by Agrobacterium tumefaciens by analysing their ability to regulate the expression of the uidA reporter gene in response to water deficit mimicked by polyethylene glycol (−2.0 MPa) and low relative humidity treatments.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Coffe; Water deficit; Genetic transformation; Haplotypes; Reporter gene.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1084513
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Efficient Agrobacterium tumefaciens-mediated transformation of Malus zumi (Matsumura) Rehd using leaf explant regeneration system Electron. J. Biotechnol.
Xu,Jin; Wang,Yu Zhen; Xia Yin,Heng; Jing Liu,Xiao.
Malus zumi is known as an excellent dwarfing apple rootstock occurring in natural or arid/semiarid soil or salina. Gene manipulation of M. zumi through transgenic technology can modify plant feature for further improvement fruit tree production by grafting the scion on a transgenic rootstock. Here, we report the establishment of an efficient, in vitro, shoot regeneration system and Agrobacterium tumefaciens- mediated transformation from the leaf explants for Malus zumi (Matsumura) Rehd. Leaf explants were infected with Agrobacterium strains containing nptII and gus gene. The highest frequency of shoot regeneration was obtained on MS medium containing 500 mg l-1 Lactalbumin hydrolysate, 30 g l-1 fructose, supplemented with 3.0 mg l-1 BA, 0.2 mg l-1...
Tipo: Journal article Palavras-chave: Agrobacterium tumefaciens; Apple rootstock; Genetic transformation.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100003
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Eggplant (Solanum melongena L.): tissue culture, genetic transformation and use as an alternative model plant Acta Botanica
Magioli,Claudia; Mansur,Elisabeth.
Eggplant is an agronomically important non-tuberous solanaceous crop grown primarily for its large oval fruit. In popular medicine, eggplant is indicated for the treatment of several diseases, including diabetes, arthritis, asthma and bronchitis. Eggplant is susceptible to a number of diseases and pests capable of causing serious crop losses. This problem has been addressed by hybridizing eggplant with wild resistant Solanum species, which present a wide genetic diversity and are source of useful agronomic traits. The application of in vitro methodologies to eggplant has resulted in considerable success. Eggplant tissues present a high morphogenetic potential that is useful for developmental studies as well as for establishing biotechnological approaches...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum melongena L.; Biotechnology; Tissue culture; Genetic transformation; Model plant.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062005000100013
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Ex vitro hairy root induction in detached peanut leaves for plant-nematode interaction studies. Repositório Alice
GUIMARAES, L. A.; PEREIRA, B. M.; ARAUJO, A. C. G.; GUIMARAES, P. M.; BRASILEIRO, A. C. M..
2017
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Expansin like-B; Meloidogyne; Arachis; Agrobacterium rhizogenes; Genetic transformation.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1070577
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Expression of an osmotin-like protein from Solanum nigrum confers drought tolerance in transgenic soybean. Repositório Alice
WEBER, R. L. M. W.; WIEBKE-STROHM, B.; BREDEMEIER, C.; MARGIS-PINHEIRO, M.; BRITO, G. G. de; RECHENMACHER, C.; BERTAGNOLLI, P. F.; SÁ, M. E. L. de; CAMPOS, M. de A.; AMORIM, R. M. S. de; BENEVENTI, M. A.; MARGIS, R.; GROSSI-DE SA, M. F.; BODANESE-ZANETTINI, M. H..
2014
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Abiotic stress; Bombardment; Drought tolerance; Genetic transformation; Glycine max; Osmotin; Water deficit.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1012379
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Fatores que influenciam o processo de transformação genética em soja via Agrobacterium tumefaciens. Repositório Alice
CRUZ, G. E. N.; SILVA, M. H. P.; MOLINARI, M. D. C.; MARIN, S. R. R.; MERTZ-HENNING, L. M.; NEPOMUCENO, A. L..
bitstream/item/204725/1/p-81.pdf
Tipo: Capítulo em livro científico (ALICE) Palavras-chave: Genética; Soja; Agrobacterium Tumefaciens; Soybeans; Genetic transformation; Agrobacterium.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114376
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Gene silencing: concepts, applications, and perspectives in woody plants Scientia Agricola
Souza,Amancio José de; Mendes,Beatriz Madalena Januzzi; Mourão Filho,Francisco de Assis Alves.
RNA interference, transcriptional gene silencing, virus induced gene silencing, and micro RNAs comprise a series of mechanisms capable of suppressing gene expression in plants. These mechanisms reveal similar biochemical pathways and appear to be related in several levels. The ability to manipulate gene silencing has produced transgenic plants able to switch off endogenous genes and invading nucleic acids. This powerful biotechnological tool has provided plant breeders and researchers with great opportunity to accelerate breeding programs and developmental studies in woody plants. This research work reports on gene silencing in woody plants, and discuss applications and future perspectives.
Tipo: Info:eu-repo/semantics/article Palavras-chave: RNAi; MiRNA; SiRNA; Genetic transformation; Virus resistance.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000600014
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Genetic engineering in cowpea (Vigna unguiculata): history, status and prospects. Repositório Alice
CITADIN, C. T.; IBRAHIM, A. B.; ARAGAO, F. J. L..
bitstream/item/179859/1/Genetic-engineering-in-Cowpea-Vigna-unguiculata-History-status-and-prospects.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Cowpea; Mendelian segregation; Vigna Unguiculata; Agronomic traits; Genetic transformation.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/951864
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Genetic transformation of sorghum (Sorghum bicolor L.) by Agrobacterium tumefaciens and particle bombardment. Repositório Alice
VASCONCELOS, M. J. V.; FONTES, M.A.; CARNEIRO, A. A.; CARNEIRO, N. P.; PAIVA, E.; CARVALHO, C.H.S.; LOPES, M.A..
1999
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sorgo; Transformacao genetica; Sorghum bicolor; Agrobacterium tumefaciens; Genetic transformation.
Ano: 1999 URL: http://www.alice.cnptia.embrapa.br/handle/doc/483387
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Genetic transformation of tropical maize (Zea mays L.) by Agrobacterium tumefaciens and particle bombardment. Repositório Alice
VASCONCELOS, M. J. V.; CARNEIRO, A. A.; FONTES, M.A.; CARNEIRO, N. P.; PAIVA, E.; CARVALHO, C.H.S.; LOPES, M.A..
1999
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Milho; Transformacao genetica; Tropical Zea mays; Agrobacterium tumefaciens; Maize; Genetic transformation.
Ano: 1999 URL: http://www.alice.cnptia.embrapa.br/handle/doc/483388
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Germinating the seeds of success in the semi-arid tropics Open Agri
William D..
Palavras-chave: Pigeonpeas; Sorghum; Millets; Chickpeas; Transgenics; Biotechnology; Electrophoresis; Groundnuts; Agroecosystems; Genetic transformation.
Ano: 2006 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4145
Registros recuperados: 52
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