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Registros recuperados: 34
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Agrobacterium-mediated transformation of Citrus sinensis and Citrus limonia epicotyl segments Scientia Agricola
Almeida,Weliton Antonio Bastos de; Mourão Filho,Francisco de Assis Alves; Mendes,Beatriz Madalena Januzzi; Pavan,Alexandra; Rodriguez,Adriana Pinheiro Martinelli.
Genetic transformation allows the release of improved cultivars with desirable characteristics in a shorter period of time and therefore may be useful in citrus breeding programs. The objective of this research was to establish a protocol for genetic transformation of Valencia and Natal sweet oranges (Citrus sinensis L. Osbeck) and Rangpur lime (Citrus limonia L. Osbeck). Epicotyl segments of germinated in vitro plantlets (three weeks in darkness and two weeks in a 16-h photoperiod) were used as explants. These were co-cultivated with Agrobacterium tumefaciens strain EHA-105 and different experiments were done to evaluate the transformation efficiency: explants were co-cultivated with Agrobacterium for one, three or five days; explants were incubated with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic transformation; Organogenesis; Micrografting; Improvement.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000100005
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Applications of genomics to rice breeding International Rice Research Institute
Mackill, D. J..
p.9-15
Palavras-chave: Breeding; Genome analysis; Gene mapping; DNA sequencing; Genetic markers; Genetic markers; Genetic transformation; Gene expression.
Ano: 2003 URL: http://hdl.handle.net/123456789/1307
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Biofortification: a global challenge program International Rice Research Institute
Graham, R. D..
p.4-8
Palavras-chave: Fortification; Research; Breeding; Genome analysis; Genetic transformation.
Ano: 2003 URL: http://hdl.handle.net/123456789/1306
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Biological activity of lectins from grain legumes and garlic against the legume pod borer, Helicoverpa armigera Open Agri
Arora, R.,( Richa Arora ).
Palavras-chave: Garlic; Pigeonpeas; Proteins; Transgenics; Transgenic plants; Chickpeas; Genes; Enzymes; Animal developmental stages; Genetic transformation.
Ano: 2005 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3230
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Changes in peroxidase and polypeptide profiles in Nicotiana tabacum L. transformed with Agrobacterium rhizogenes Ciência Rural
Echeverrigaray,Sergio.
Morphological and proteic modifications in Nicotiana tabacum L. transformed by Agrobacterium rhizogenes were evaluated by the comparison of normal and transformant plants regenerated from hairy-roots formed by the strains A4 or IB-642 of A. rhizogenes. Changes in apical dominance were observed in IB-642 transformants, which exhibited an abnormal development of axiliary buds. The electrophoretic analysis indicated an increase in peroxidase activity and the induction of several isozymes of this complex in the transformants. The SDS-PAGE patterns comparison allows to identify several changes, specially, the increase in 31-33 and 54 kD polypeptides in the transformants.. Biochemical analysis suggests the induction of a pathogen or stress like response of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic transformation; Agrobacterium rhizogenes; Nicotiana tabacum; Electrophoretic analysis.
Ano: 1995 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781995000200009
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Coffee biotechnology Braz. J. Plant Physiol.
De Los Santos-Briones,César; Hernández-Sotomayor,S. M. Teresa.
In the last three decades, interest has turned to in vitro cell culture in different areas of coffee research. In vitro techniques have been applied not only for coffee improvement through genetic transformation but also to study various aspects in coffee cells such as chemical (caffeine synthesis and the production of coffee aroma), physiological and more recently, biochemical aspects. The most important advances obtained to date on in vitro coffee techniques in fields like biochemistry, physiology, regeneration systems and genetic engineering, are presented and discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea; Coffee; Genetic transformation; In vitro tissue culture; Regeneration; Somatic embryogenesis.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100015
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Efficient Agrobacterium tumefaciens-mediated transformation of Malus zumi (Matsumura) Rehd using leaf explant regeneration system Electron. J. Biotechnol.
Xu,Jin; Wang,Yu Zhen; Xia Yin,Heng; Jing Liu,Xiao.
Malus zumi is known as an excellent dwarfing apple rootstock occurring in natural or arid/semiarid soil or salina. Gene manipulation of M. zumi through transgenic technology can modify plant feature for further improvement fruit tree production by grafting the scion on a transgenic rootstock. Here, we report the establishment of an efficient, in vitro, shoot regeneration system and Agrobacterium tumefaciens- mediated transformation from the leaf explants for Malus zumi (Matsumura) Rehd. Leaf explants were infected with Agrobacterium strains containing nptII and gus gene. The highest frequency of shoot regeneration was obtained on MS medium containing 500 mg l-1 Lactalbumin hydrolysate, 30 g l-1 fructose, supplemented with 3.0 mg l-1 BA, 0.2 mg l-1...
Tipo: Journal article Palavras-chave: Agrobacterium tumefaciens; Apple rootstock; Genetic transformation.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100003
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Eggplant (Solanum melongena L.): tissue culture, genetic transformation and use as an alternative model plant Acta Botanica
Magioli,Claudia; Mansur,Elisabeth.
Eggplant is an agronomically important non-tuberous solanaceous crop grown primarily for its large oval fruit. In popular medicine, eggplant is indicated for the treatment of several diseases, including diabetes, arthritis, asthma and bronchitis. Eggplant is susceptible to a number of diseases and pests capable of causing serious crop losses. This problem has been addressed by hybridizing eggplant with wild resistant Solanum species, which present a wide genetic diversity and are source of useful agronomic traits. The application of in vitro methodologies to eggplant has resulted in considerable success. Eggplant tissues present a high morphogenetic potential that is useful for developmental studies as well as for establishing biotechnological approaches...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum melongena L.; Biotechnology; Tissue culture; Genetic transformation; Model plant.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062005000100013
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Gene silencing: concepts, applications, and perspectives in woody plants Scientia Agricola
Souza,Amancio José de; Mendes,Beatriz Madalena Januzzi; Mourão Filho,Francisco de Assis Alves.
RNA interference, transcriptional gene silencing, virus induced gene silencing, and micro RNAs comprise a series of mechanisms capable of suppressing gene expression in plants. These mechanisms reveal similar biochemical pathways and appear to be related in several levels. The ability to manipulate gene silencing has produced transgenic plants able to switch off endogenous genes and invading nucleic acids. This powerful biotechnological tool has provided plant breeders and researchers with great opportunity to accelerate breeding programs and developmental studies in woody plants. This research work reports on gene silencing in woody plants, and discuss applications and future perspectives.
Tipo: Info:eu-repo/semantics/article Palavras-chave: RNAi; MiRNA; SiRNA; Genetic transformation; Virus resistance.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000600014
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Germinating the seeds of success in the semi-arid tropics Open Agri
William D..
Palavras-chave: Pigeonpeas; Sorghum; Millets; Chickpeas; Transgenics; Biotechnology; Electrophoresis; Groundnuts; Agroecosystems; Genetic transformation.
Ano: 2006 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4145
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Histological study of shoot organogenesis in melon (Cucumis melo L.) after genetic transformation National Institute of Agronomic Research
Chovelon, V.; Restier, V.; Dogimont, C.; Aarouf, J..
Leave and cotyledon explants from two melon genotypes ‘Vedrantais’ and ‘Paul’ were cultured on different media and their regeneration was followed during 60 days. High frequency of plant regeneration was observed with ‘Vedrantais’ cotyledons cultured on MS medium added with BAP and 2-iP. Using this protocol, Agrobacterium mediated transformation was developed and a significant reduction of the plant regeneration rate was observed. Explants histological analysis revealed the presence of transformed meristematic areas but a rapid and premature cell vacuolization was observed. During plant regeneration, meristematic cells were affected by important cytological damages: cell dislocation accompanied by cell death and final collapsus. This disruption of...
Tipo: Poster Palavras-chave: Cucumis melo; Agrobacterium tumefaciens; GUS uidA; Genetic transformation; Regeneration.
Ano: 2008 URL: http://hdl.handle.net/2174/285
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Improving the genetic transformation efficiency of Cucumis melo subsp. melo ‘Piel de Sapo’ via Agrobacterium National Institute of Agronomic Research
Castelblanque, L.; Marfa, V.; Claveria, E.; Martinez, I.; Perez-Grau, L.; Dolcet-Sanjuan, R..
The effects of five Agrobacterium strains, two selection agents, and co-culture conditions were evaluated on the transformation efficiency of three melon lines (‘T111’, ‘PS8’, ‘PNY’) belonging to the cultivar Cucumis melo subsp. melo ‘Piel de Sapo’, the C. melo subsp. melo ‘Védrantais’ line, and the accession C. melo subsp. agrestis ‘PI 161375’. Transformation efficiencies for accession ‘PI 161375’ (10.6 %) were higher than those obtained for ‘Piel de Sapo’ (0.1 %) or ‘Védrantais’ (3.1 %). Among the ‘Piel de Sapo’ lines, the highest rates of transformation were observed with ‘T111’. Selection with pmi solved the problem of escapes encountered with nptII on ‘Piel de Sapo’. Results on the number of explants with GUS expression indicated that co-culture at...
Tipo: Poster Palavras-chave: Melon; Muskmelon; Near Isogenic Lines (NILs); Pmi; Agrobacterium; Genetic transformation; Piel de Sapo; Cucumis melo subsp. melo; Transformation efficiency; Agrobacterium infection; Regeneration.
Ano: 2008 URL: http://hdl.handle.net/2174/284
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In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia Scientia Agricola
Almeida,Weliton Antonio Bastos de; Mourão Filho,Francisco de Assis Alves; Mendes,Beatriz Madalena Januzzi; Rodriguez,Adriana Pinheiro Martinelli.
Exogenous genes can be introduced in plants by genetic transformation techniques. However, an efficient tissue culture system with high rates of plant recovery is necessary for gene introduction. This work aimed to define organogenesis and plant regeneration protocols for sweet orange varieties Natal, Valencia and Hamlin (Citrus sinensis L. Osbeck) and Rangpur lime (Citrus limonia L. Osbeck) which can be used in plant transformation experiments. Seeds of which teguments were removed, were germinated in vitro and maintained in the dark for three weeks, followed by one week at 16-h photoperiod (40 µmol m-2 s-1) and 27 ± 2°C. Organogenesis induction was done by introducing epicotyl segments in MT medium with 25 g L-1 sucrose and different BAP concentrations....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adventitious bud; Rooting; Genetic transformation.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000100004
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Induction of transgenic hairy roots in soybean genotypes by Agrobacterium rhizogenes‑mediated transformation PAB
Weber,Ricardo Luís Mayer; Bodanese‑Zanettini,Maria Helena.
The objective of this work was to perform the screening of soybean genotypes as to their ability to respond to the induction of hairy roots by Agrobacterium rhizogenes‑mediated transformation. Four Brazilian soybean cultivars (BRSMG 68 Vencedora, BRS 137, Embrapa 48, and MG/BR 46 Conquista) and two North American ones adapted to Brazilian cropping conditions (Bragg and IAS‑5) were screened for their capacity to respond to A. rhizogenes in protocols for in vitro hairy root culture and ex vitro composite plant production. Four‑day‑old seedlings with uniform size were injected with A. rhizogenes harboring the plasmid p35S‑GFP. Seedlings expressing green fluorescent protein (GFP) in at least one hairy root were used to determine the transformation frequency....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Composite plants; Genetic transformation; Hairy roots culture; Strain K599.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000900014
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Influence of antibiotics on embryogenic tissue and Agrobacterium tumefaciens suppression in soybean genetic transformation Bragantia
Wiebke,Beatriz; Ferreira,Fabricio; Pasquali,Giancarlo; Bodanese-Zanettini,Maria Helena; Droste,Annette.
The influence of different antibiotic treatments in soybean genetic transformation was evaluated. First, an assay was performed to verify how different antibiotic treatments affect soybean embryogenic tissues. The effect of carbenicillin at 500 mg L-1 was genotype-dependent. This antibiotic did not affect embryo survival of cv. IAS5, but a three-fold increase of embryo proliferation was observed for cv. Bragg, when compared to the control. On the other hand, cefotaxime at 350 and 500 mg L-1 caused death of embryogenic tissues of both cultivars. Finally, the association of cefotaxime (250 mg L-1) + vancomycin (250 mg L-1) did not affect negatively the somatic embryos of tested cultivars until 63 days of treatment. Thereafter, a second experiment was carried...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agrobacterium tumefaciens; Carbenicillin; Cefotaxime; Genetic transformation; Glycine max; Vancomycin.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052006000400002
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Isolation of a cotton NADP(H) oxidase homologue induced by drought stress PAB
NEPOMUCENO,ALEXANDRE LIMA; STEWART,JAMES MCD; OOSTERHUIS,DERRICK; TURLEY,RICKIE; NEUMAIER,MORMAN; FARIAS,JOSÉ RENATO BOUÇAS.
The aim of this study was to identify and isolate genes that are differentially expressed in four selected cotton (Gossypium hirsutum L.) genotypes contrasting according to their tolerance to water deficit. The genotypes studied were Siokra L-23, Stoneville 506, CS 50 and T-1521. Physiological, morphological and developmental changes that confer drought tolerance in plants must have a molecular genetic basis. To identify and isolate the genes, the mRNA Differential Display (DD) technique was used. Messenger RNAs differentially expressed during water deficit were identified, isolated, cloned and sequenced. The cloned transcript A12B15-5, a NADP(H) oxidase homologue, was up regulated only during the water deficit stress and only in Siokra L-23, a drought...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Messenger RNA; Gene expression; Genetic transformation; Water deficit.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000700015
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Laboratory Protocols 2008: Maize Nutrition Quality and Plant Tissue Analysis Laboratory AgEcon
Gallicia, L.; Nurit, E.; Rosales, A.; Palacios-Rojas, N..
Tipo: Report Palavras-chave: Zea mays; Maize; Wheats; Triticum; Transgenic plants; Genetic engineering; Gene transfer; Genetic transformation; DNA hybridization; Phenotypes; Selection; Tissue culture; In vitro culture; Biolistics; Research projects; Methods; Molecular cloning; PCR; Crop Production/Industries; F02; F30.
Ano: 2009 URL: http://purl.umn.edu/56177
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Molecular strategies to use nuclear male sterility in plant hybrid breeding International Rice Research Institute
Li, X. Q.; Yuan, L. P..
p.10-12
Palavras-chave: Male sterility; Genes; Genetic transformation; Plastids; Gene expression; Promoters; Recombination; Plant breeding; Molecular genetics.
Ano: 2004 URL: http://hdl.handle.net/123456789/924
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pGVG: a new Gateway-compatible vector for transformation of sugarcane and other monocot crops Genet. Mol. Biol.
Guidelli,Giovanna V.; Mattiello,Lucia; Gallinari,Rafael H.; Lucca,Paulo Cezar de; Menossi,Marcelo.
Abstract The successful development of genetically engineered monocots using Agrobacterium-mediated transformation has created an increasing demand for compatible vectors. We have developed a new expression vector, pGVG, for efficient transformation and expression of different constructs for gene overexpression and silencing in sugarcane. The pCAMBIA2300 binary vector was modified by adding Gateway recombination sites for fast gene transfer between vectors and the maize polyubiquitin promoter Ubi-1 (ZmUbi1), which is known to drive high gene expression levels in monocots. Transformation efficiency using the pGVG vector reached up to 14 transgenic events per gram of transformed callus. Transgenic plants expressing the β-glucuronidase (GUS) reporter gene...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Monocots; Sugarcane; Vector; Gateway technology; Genetic transformation.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300450
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PHLOEM PROMOTERS IN TRANSGENIC SWEET ORANGE ARE DIFFERENTIALLY TRIGGERED BY Candidatus Liberibacter asiaticus Rev. Bras. Frutic.
MIYATA,LUZIA YURIKO; HARAKAVA,RICARDO; ATTÍLIO,LÍSIA BORGES; MENDES,BEATRIZ MADALENA JANUZZI; LOPES,JOÃO ROBERTO SPOTTI; COLETTA-FILHO,HELVÉCIO DELLA; SOUZA,ALESSANDRA ALVES DE; MOURÃO FILHO,FRANCISCO DE ASSIS ALVES.
ABSTRACT The use of promoters preferentially expressed in specific plant tissues is a desirable strategy to search for resistance for pathogens that colonize these tissues. The bacterium Candidatus Liberibacter asiaticus (Las), associated with huanglongbing disease (HLB) of citrus, colonizes phloem vessels. Some promoters, besides conferring tissue-specific expression, can also respond to the presence of the pathogen. The objective of the present study was to verify if the presence of Las could modulate the activation of the phloem-specific promoters AtPP2 (Arabidopsis thaliana phloem protein 2), AtSUC2 (A. thaliana sucrose transporter 2) and CsPP2 ( pCitrus phloemrotein 2), known to be expressed in Citrus sinensis phloem. ‘Hamlin’ sweet orange plants...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus sinensis; Diaphorina citri; Genetic transformation; GUS; Huanglongbing; Quantitative real-time PCR.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000300101
Registros recuperados: 34
Primeira ... 12 ... Última
 

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