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Registros recuperados: 11
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Cytogenetics of Chilean angiosperms: Advances and prospects RChHN
JARA-SEGUEL,PEDRO; URRUTIA,JONATHAN.
Cytogenetic data on Chilean angiosperms have been reported since at least eight decades ago; however, much of this information is disperse in diverse sources and is not readily available as a comprehensive document that allows having a general vision on advances and gaps in this matter. The goal of this paper is to summarize the advances and prospets on cytogenetic studies of the Chilean angiosperms based on compiled publications from 1929 to 2010. We found 78 publications supplied by four groups of Chilean researchers and some foreign specialists. Cytogenetic data have been reported for 139 Chilean angiosperm species (2.8 % of the total), which belong to 58 genera and 34 families. During 2001-2010 there was an increase in the number of publications, being...
Tipo: Journal article Palavras-chave: Chromosome banding; Chromosome number; Genome size; Karyotype morphology.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2012000100001
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Detection of diploid males in a natural colony of the cleptobiotic bee Lestrimelitta sp (Hymenoptera, Apidae) Genet. Mol. Biol.
Tavares,Mara Garcia; Carvalho,Carlos Roberto; Soares,Fernanda Aparecida Ferrari; Fernandes,Anderson.
When working at quantifying the genome size of stingless bees, it was observed that males of Lestrimelitta sp possessed the same amount of nuclear DNA as the females. Thus, we used flow cytometry (FCM) and cytogenetic analysis to confirm the ploidy of these individuals. The males analyzed proved to be diploid, since, through cytometric analysis, it was demonstrated that the mean genome size of both males and females was the same (C = 0.463 pg), and, furthermore, cytogenetic analysis demonstrated that both had 2n = 28 chromosomes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetic; Flow cytometry; Genome size; Karyotype; Stingless bee.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300019
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DNA content estimation of Fig and Black Mulberry using flow cytometry Rev. Bras. Frutic.
Dalkiliç,Zeynel; Dalkiliç,Gonca Günver.
Abstract In this study, fig and black mulberry DNA contents were estimated using DAPI fluorescence stain in flow cytometry. The 2C DNA contents of the fig and black mulberry were found as 0.82 pg and 8.34 pg, respectively. The calculated 1C value of genome size of fig is 401.8 Mbp and that of black mulberry is 4086.6 Mbp. The ratio of 2C DNA content and 1C genome of the black mulberry was 10.17 times that of the fig although fig is diploid and black mulberry is decosaploid.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ficus carica; Morus nigra; DAPI; DNA content; Flow cytometry; Genome size.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000200801
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Genome analysis of thirteen Colombian clostridial strains by pulsed field gel electrophoresis Electron. J. Biotechnol.
Quilaguy Ayure,Diana Milena; Suárez Moreno,Zulma Rocío; Aristizábal Gutierrez,Fabio Ancizar; Bernal Morales,Jose Mauricio; Montoya Castaño,Dolly.
Pulsed field gel electrophoresis was used for estimating the size of the genome and evaluating the presence of megaplasmids in 13 native Colombian solventogenic Clostridium strains. DNA preparation and purification were optimised for obtaining differentiated restriction fragments in electrophoresis. Genomic DNA was digested with ApaI, Eco52I, SmaI and XhoI enzymes. Estimated genome size for native strains ranged from 4.0 to 4.2 mega base pairs. Larger sized plasmids were detected and the presence of genes related to megaplasmid pSOL1 was determined by polymerase chain reaction. adc gene region amplification suggested that genes related to solventogenesis in native strains may be located in an extra-chromosomal element. Determining genome size provides...
Tipo: Journal article Palavras-chave: Clostridium; Genome size; PFGE; PSOL1.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500009
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Genome size and ploidy variations in pearl millet (Pennisetum glaucum) and napier grass (Pennisetum purpureum) genotypes Acta Agron. (Palmira)
Animasaun,David Adedayo; Morakinyo,Joseph Akintade; Mustapha,Oba Toyin; Krishnamurthy,Rama.
Abstract Pennisetum glaucum (Pearl millet) and P. purpureum (Napier grass) are economically important members of the genus Pennisetum . The knowledge of variability in chromosome number, size and genomic content of the species could provide clues on the mechanisms responsible for decrease or increase in genomic content in the evolutionary pathway. In the present study, twenty nine genotypes consisting of 24 pearl millet and 5 Napier grass were assessed for inter- and intra-variations in chromosome number, ploidy status and genome content. Conventional cytogenetic was used for chromosome counting and flow cytometry technique for assessing genomic contents of the genotypes. Pearl millet genotypes were diploid (2n = 14) while Napier grass were mainly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genome size; Flow cytometry; Napier grass; Pearl millet; Pennisetum; Ploidy analysis.
Ano: 2019 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122019000400299
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Genome size of three Brazilian flies from the Sciaridae family Genet. Mol. Biol.
Saccuti,Cecília Ferreira; Soares,Maria Albertina de Miranda; Falco,José Ricardo Penteado; Fernandez,Maria Aparecida.
We determined the genome size of the Brazilian sciarid flies Bradysia hygida, Rhynchosciara americana and Trichosia pubescens (Diptera: Sciaridae) using absorbance measurements of Feulgen-stained nuclei belonging to these species (and chicken erythrocytes as a standard) to calculate the amount of DNA in picograms (pg) and the number of base pairs (bp), or C-value, for each of these species. The C-values were: 3 x 10(8) bp (0.31 pg) for B. hygida; 3.6 x 10(8) bp (0.37 pg) for R. americana; and 1 x 10(9) bp (1.03 pg) for T. pubescens. The sciarids investigated in this work had considerably higher C-values than the average for previously described dipteran species, including D. melanogaster.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Genome size; Feulgen reaction; Sciaridae; Bradysia hygida; Rhynchosciara americana; Trichosia pubescens; Brazilian flies.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500015
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Het Dryopteris affinis-complex in Nederland Naturalis
Hovenkamp, P.; Hendrikx, B.; Roskam, H.; Winter, W. de.
With the help of flow-cytometric analysis of nuclear DNA content and morphology, we establish the presence in the Netherlands of two taxa of the Dryopteris affinis complex: diploid D. affinis subsp. affinis, triploid D. affinis subsp. borreri, and the hybrids D × complexa (tetraploid) and D. × critica (pentaploid). Onderzoek aan kern-DNA gewicht en morfologie van recente vondsten van het Dryopteris affinis-complex wijst uit dat in Nederland twee taxa en twee hybrides met redelijke zekerheid zijn gevonden: dediploïde D. affinis subsp. affinis, de triploïde D. affinis subsp. borreri, en de hybriden D × complexa (tetraploïd) en D. × critica (pentaploïd). We analyseren een aantal onderscheidende kenmerken en maken de keuze om deze taxa te behandelen als...
Tipo: Article / Letter to the editor Palavras-chave: Dryopteris affinis; Soortscomplex; Species complex; Uitbreiding; Range expansion; Genoomgrootte; Genome size; Cytotypes; Polyploidie; Polyploidy; Morfometrie; Morphometrics.
Ano: 2018 URL: http://www.repository.naturalis.nl/record/669050
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Karyotype and nuclear DNA content of hexa-, octo-, and duodecaploid lines of Bromus subgen. Ceratochloa Genet. Mol. Biol.
Klos,Joanna; Sliwinska,Elwira; Kula,Adam; Golczyk,Hieronim; Grabowska-Joachimiak,Aleksandra; Ilnicki,Tomasz; Szostek,Krzysztof; Stewart,Alan; Joachimiak,Andrzej J..
The subgenus Ceratochloa of the genus Bromus includes a number of closely related allopolyploid forms or species that present a difficult taxonomic problem. The present work combines data concerning chromosome length, heterochromatin distribution and nuclear genome size of different 6x, 8x and 12x accessions in this subgenus. Special attention is paid to the karyotype structure and genomic constitution of duodecaploid plants recently found in South America. Hexaploid lineages possess six almost indistinguishable genomes and a nuclear DNA content between 12.72 pg and 15.10 pg (mean 1Cx value = 2.32 pg), whereas octoploid lineages contain the same six genomes (AABBCC) plus two that are characterized by longer chromosomes and a greater DNA content (1Cx = 4.47...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Karyotype; C-banding; Heterochromatin; Flow cytometry; Genome size.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300016
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rDNA mapping, heterochromatin characterization and AT/GC content of Agapanthus africanus (L.) Hoffmanns (Agapanthaceae) Anais da ABC (AABC)
REIS,ARYANE C.; FRANCO,ANA LUIZA; CAMPOS,VICTÓRIA R.; SOUZA,FLÁVIA R.; ZORZATTO,CRISTIANE; VICCINI,LYDERSON F.; SOUSA,SAULO M..
ABSTRACT Agapanthus (Agapanthaceae) has 10 species described. However, most taxonomists differ respect to this number because the great phenotypic plasticity of the species. The cytogenetic has been an important tool to aid the plant taxon identification, and to date, all taxa of Agapanthus L'Héritier studied cytologically, presented 2n = 30. Although the species possess large chromosomes, the group is karyologically little explored. This work aimed to increase the cytogenetic knowledge of Agapanthus africanus (L.) Hoffmanns by utilization of chromosome banding techniques with DAPI / CMA3 and Fluorescent in situ Hybridization (FISH). In addition, flow cytometry was used for determination of DNA content and the percentage of AT / GC nitrogenous bases....
Tipo: Info:eu-repo/semantics/article Palavras-chave: African lily; Chromosome banding; Cytotaxonomy; Flow cytometry; Genome size; Molecular cytogenetics.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501727
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Recent advances in EST sequencing in Crassostrea gigas: towards the sequencing of the Pacific oyster genome ArchiMer
Boudry, Pierre; Huvet, Arnaud; Lapegue, Sylvie; Sauvage, Christopher; Tanguy, A.; Moraga, D.; Goostrey, A.; Prunet, P.; Boulo, Viviane; Gueguen, Y.; Moal, Jeanne; Fleury, Elodie; Samain, Jean-francois; Mathieu, M.; Favrel, P..
Rationale for EST and genome sequencing of the Pacific oyster: • its membership of the Lophotrochozoa, an little studied branch of the Eukaryotes, • its worldwide aquacultural importance (4.2 million metric tons, worth 3.5 billion US dollars), • its high fecundity, with concomitantly high DNA polymorphism, • its use as a biosensor of coastal pollution. In 2004, an international community of biologists, the Oyster Genome Consortium (OGC) led by D. Hedgecock (University of Southern California) and uniting 70 participants from 10 countries, first presented the Pacific oyster Crassostrea gigas as a genome-sequencing candidate to Joint Genome Institute (JGI, USA) (Hedgecock et al., 2005). Besides this project (that remains to be accepted), several EST...
Tipo: Text Palavras-chave: Characteristics; Genome size; Genome; Crassostrea gigas; Oyster; Genetic.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3414.pdf
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台灣芭蕉倍數性的研究 Taiwan Agricultural Research Institute
邱輝龍; 陳榮芳; 許圳塗; 張淯茱; Hui-Lung Chiu; Long-Fang O. Chen; Chou-Tou Shii; Yu-Chu Chang.
[[abstract]]本研究證實台灣芭蕉 [Musa formosana (Warb.) Hayata] 的染色體數為2n = 22,綜合先前形態特性調查資料,將台灣芭蕉歸類為Eumusa 宗的二倍體野生種香蕉植物。以流式細胞儀測得台灣芭蕉體細胞核內DNA 含量為1.387 pg/2C,基因組 大小約為669.23 Mbp/2C,相較於尖蕉 (M. acuminata) 的A 基因組與拔蕉 (M. balbisiana) 的B 基因組大小,分別高出18%與26%。基因組大小的資料不僅可應用於不同物種的鑑定,尚可預測基因組間植株雜交後裔的基因組組成,而應用於雜交後裔的早期檢定。
Palavras-chave: 台灣芭蕉 染色體數 基因組大小 流式細胞技術 野生香蕉 Musa formosana; Chromosome number; Genome size; Fl ow cytometry; Wild banana [[classification]]9.
Ano: 2010
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