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Registros recuperados: 33
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Bioensaios de toxicidade genética com plantas superiores Tradescantia (MCN e SHM), milho e soja. Repositório Alice
RODRIGUES, G. S..
Para se evitarem agentes mutagênicos no ambiente são necessários indicadores sensíveis para detectar todo o espectro desses compostos. Tais sistemas indicadores tem sido definidos como testes de mutagênese de tipo II, ou seja, aqueles que apresentam alta sensibilidade e baixa especificidade. A maioria dos bioensaios vegetais são considerados testes do tipo II, com especial referencia para os ensaios do micronúcleo e do pelo estaminal em Tradescantia (comelinacea), e o ensaio do grão de pólen ceroso em milho. Outro bioensaio vegetal de interesse para o monitoramento de agentes mutagênicos ambientais e o teste do mosaicismo em soja, que permite especulação sobre o mecanismo envolvido na toxicidade genética. O bioensaio não exige qualquer instrumentação...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Toxidez; Mutação; Milho; Soja; Tradescantia; Bioassays; Soybean; Corn; Mutagenesis; Genotoxicity.
Ano: 1998 URL: http://www.alice.cnptia.embrapa.br/handle/doc/13061
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Bioensaios de toxicidade genética com Tradescantia. Infoteca-e
RODRIGUES, G. S..
Resumo: Dentre a variedade de bioensaios disponíveis para estudos de detecção e monitoração dos efeitos tóxicos genéticos de substâncias e ambientes contaminados, os bioensaios que empregam a planta Tradescantia destacam-se pela sensibilidade e facilidade de realização de estudos de curto prazo in situ. Este trabalho apresenta uma revisão extensiva dos estudos de mutagênese ambiental empregando Tradescantia como bioindicador, e é complementado com resultados experimentais e indicações metodológicas.
Tipo: Documentos (INFOTECA-E) Palavras-chave: Bioensaio; Toxidez; Mutação; Agrotóxico; Tradescantia; Bioassays; Mutagenesis; Genotoxicity; Pesticides.
Ano: 1999 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/1051547
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Cd, Cu, and Mn from Uruguay River Basin in Uruguaiana, RS, Brazil, and their toxicological potential for human leukocyte Biological Sciences
Costa, Gislaine Rezer; Rocha, Mariana Balhego; Querol, Marcus Vinicius Morini; Dal Magro, Jacir; Machado, Michel Mansur; Oliveira, Luís Flávio Souza de.
This study assessed the limnology from the Medium Uruguay River Basin in Uruguaiana, Brazil, with a focus on the concentration of heavy metals (Cd, Cu, and Mn), to assess the toxicological potential (cytotoxicity and genotoxicity) for humans using as biological matrix of study human leukocyte cells. The conductivity, resistivity, and dissolved O2 levels exceeded the limits recommended by the National Environmental Council (Conselho Nacional do Meio Ambiente - CONAMA). The percentage of non-viable human leukocyte cells exposed to water samples was approximately 20% higher than that of the negative control (<3%), but similar to the positive control. The DNA damage index was high for all heavy metal concentrations assayed when compared to the negative...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.00.00.00-6 ecotoxicology; Genotoxicity; Limnology; Heavy metals; Uruguay River Basin Ecotoxicologia.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31998
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Chronic dietary exposure of zebrafish to PAH mixtures results in carcinogenic but not genotoxic effects ArchiMer
Larcher, Thibaut; Perrichon, Prescilla; Vignet, Caroline; Ledevin, Mireille; Le Menach, Karyn; Lyphout, Laura; Landi, Laure; Clerandeau, Christelle; Lebihanic, F.; Menard, Dominique; Burgeot, Thierry; Budzinski, Helene; Akcha, Farida; Cachot, J.; Cousin, Xavier.
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants that can be present at high levels as mixtures in polluted aquatic environments. Many PAHs are potent mutagens and several are well-known carcinogens. Despite numerous studies on individual compounds, little is known about the toxicity of PAHs mixtures that are encountered in environmental situations. In the present work, zebrafish were continuously fed from 5 days post-fertilisation to 14 months post-fertilisation (mpf) with a diet spiked with fractions of either pyrolytic (PY), petrogenic light oil (LO), or petrogenic heavy oil (HO) origin at three concentrations. A decrease in survival was identified after 3 mpf in fish fed with the highest concentration of HO or LO, but not for PY. All...
Tipo: Text Palavras-chave: Polycyclic aromatic hydrocarbon; Zebrafish; Carcinogenesis; Genotoxicity; Neoplasia; Carcinoma; Toxicological pathology.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00242/35329/34108.pdf
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Chronic ethanol consumption in mice does not induce DNA damage in somatic or germ cells, evaluated by the bone marrow micronucleous assay and the dominant lethal mutation assay Biol. Res.
Ellahueñe,Manuel F; Pérez-Alzola,L Patricia; Olmedo,M Isabel.
Although alcohol is known to be a carcinogen for humans, ethanol-genotoxicity studies are incomplete. Ethanol seems not to be a bacterial mutagen, but the results are conflicting in rodent assays. We investigate the genotoxicity in the bone marrow micronucleus (MN) test and in the dominant lethal mutation (DLM) assay using two long-term ethanol exposure protocols. In the MN test, mice consumed three doses (5, 10 and 15% v/v) for 32 weeks. MN induction was compared to two control groups of 5- and 38-week-old mice (the ages of the treated mice when the treatment was initiated and when they were killed, respectively). For the three groups treated with ethanol there was no significant increase in MN induction as compared to the first control group, but...
Tipo: Journal article Palavras-chave: Dominant lethal mutation; Ethanol; Genotoxicity; Micronucleus test.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100004
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Comet assay in phytoplankton as biomarker of genotoxic effects of environmental pollution ArchiMer
Akcha, Farida; Arzul, Genevieve; Rousseau, Sabrina; Bardouil, Michele.
The alkaline comet assay was tested on different microalgae: the clinofiagellates, Karenia mikimotoi and Alexandrium minuturn, and the diatom, Chaetoceros gracilis. The microalgae were exposed during their exponential growth to the model direct genotoxicant, hydrogen peroxide (1 h, 5 and 100 pM H2O2). Following H2O2 exposure, the comet assay was validated only for K. mikimotoi for which genotoxicity was observed from the lowest tested concentration of 5 pM with a concentration-dependent effect. C. gracilis was too small in size (4 mu m) to be correctly analysed. For A. minuturn, our lysis buffer was not strong enough to digest the cellulosic thecal plates. For K. mikimotoi, the comet assay was thus applied for the study of the genotoxic effects of...
Tipo: Text Palavras-chave: Comet assay; Genotoxicity; Pesticides; Phytoplankton.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4504.pdf
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Comparative embryotoxicity and genotoxicity of the herbicide diuron and its metabolites in early life stages of Crassostrea gigas: implication of reactive oxygen species production ArchiMer
Behrens, Daphne; Rouxel, Julien; Burgeot, Thierry; Akcha, Farida.
Herbicides are one of the major classes of pollutants contaminating coastal waters over the world. Among them, diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) is a phenylurea herbicide frequently detected in oyster-producing area, known to be toxic for this important exploited non-target species. With the aim to investigate the mechanisms by which diuron displays its toxicity in oyster, the implication of both biotransformation and oxygen reactive species (ROS) production was studied considering embryotoxicity and genotoxicity as endpoints. Comparative embryotoxicity and genotoxicity of diuron and its main metabolites (DCPMU, DCPU and 3,4-DCA) were thus studied on oyster larvae by the embryo-larval bioassay on D larvae and the comet assay on trochophore...
Tipo: Text Palavras-chave: Oyster; Herbicide; Embryotoxicity; Genotoxicity; Oxidative stress; Biotransformation.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00324/43521/43053.pdf
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Cytological and cytogenic effects induced by thiabendazole on Allium cepa root meristem JBAG
Andrioli,Nancy B; Mudry,Marta D.
The toxicity and genotoxicity of thiabendazole (TBZ) in its commercial formulation Foldan® was studied in Allium cepa meristematic cells at concentrations ranging between 10 and 250 µg / ml. Our results show that exposure to TBZ induces a significant increase in the frequency of anaphase telophase chromosomal aberrations, micronuclei and binucleated cells at all the concentrations of TBZ assayed. The frequency of anaphase telophase aberrations was observed to reach a plateau at the concentration of 50 µg / ml of TBZ, which could be probably due to TBZ mitotic toxicity manifested at concentrations of 100 and 250 µg / ml. An increase was also observed in the rate of metaphase and anaphase, which was, in turn, indicative of alterations in chromatid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genotoxicity; Cytotoxicity; Mitotic index; Micronuclei; Binucleates; Thiabendazole.
Ano: 2011 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332011000200002
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Development of a comet-FISH assay for the detection of DNA damage in hemocytes of Crassostrea gigas ArchiMer
Perez Garcia, Maria Concepcion; Rouxel, Julien; Akcha, Farida.
In this work, the DNA-damaging effect of hydrogen peroxide on the structural integrity of nucleolar organizer regions (NORs) was studied for the first time by comet-FISH in the Pacific oyster Crassostrea gigas. Global DNA damage was assessed in hemocytes using an alkaline version of the comet assay. Next, NOR sensitivity was analysed by mapping major rDNA repeat unit by fluorescence in situ hybridisation (FISH) on the same comet slides. Exposure of hemocytes to 100 μM of hydrogen peroxide induced a significant increase in both DNA damage and number of FISH-signals of major ribosomal genes versus the control. Moreover, a significant positive correlation was shown between DNA damage as measured by the comet assay (percentage of DNA in comet tail) and the...
Tipo: Text Palavras-chave: Crassostrea gigas; Hydrogen peroxide; Comet assay; Comet-FISH; Genotoxicity.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00250/36157/34712.pdf
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Developmental toxicity of PAH mixtures in fish early life stages. Part I: adverse effects in rainbow trout ArchiMer
Le Bihanic, Florane; Morin, Benedicte; Cousin, Xavier; Le Menach, Karyn; Budzinski, Helene; Cachot, Jerome.
A new gravel-contact assay using rainbow trout, Oncorhynchus mykiss, embryos was developed to assess the toxicity of polycyclic aromatic hydrocarbons (PAHs) and other hydrophobic compounds. Environmentally realistic exposure conditions were mimicked with a direct exposure of eyed rainbow trout embryos incubated onto chemical-spiked gravels until hatching at 10 °C. Several endpoints were recorded including survival, hatching delay, hatching success, biometry, developmental abnormalities, and DNA damage (comet and micronucleus assays). This bioassay was firstly tested with two model PAHs, fluoranthene and benzo[a]pyrene. Then, the method was applied to compare the toxicity of three PAH complex mixtures characterized by different PAH compositions: a pyrolytic...
Tipo: Text Palavras-chave: PAH mixture; Oil extract; Pyrolytic extract; Embryotoxicity; Developmental defects; Genotoxicity.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00188/29919/28384.pdf
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DNA damage in dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) from European seas ArchiMer
Hylland, Ketil; Skei, Bjorn Borge; Brunborg, Gunnar; Lang, Thomas; Gubbins, Matthew J.; Le Goff, Jeremie; Burgeot, Thierry.
Dab (Limanda limanda) and haddock (Melanogrammus aeglefinus) were collected from coastal and offshore locations in the Baltic (dab only), North Sea (haddock from one location only) and Iceland. Blood was analysed for DNA strand breaks using the comet assay and liver samples for DNA adduct concentrations. DNA strand breaks were at background levels in dab from the two Iceland locations and from the Dogger Bank. The highest levels were observed in dab from the Firth of Forth, Ekofisk and the German Bight. Hepatic DNA adducts in dab were highest at Ekofisk, in the Baltic and Dogger Bank, below detection limit in dab from Iceland and low in dab from the Firth of Forth and German Bight. There was large variation in DNA strand breaks between locations and...
Tipo: Text Palavras-chave: ICON; Comet; DNA adducts; Genotoxicity; Haddock; Dab; European seas.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00309/42064/44971.pdf
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Dna protective effect of antioxidant apple extract. Repositório Alice
VIZZOTTO, M.; KROLOW, A. C. R..
2012
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Crystal violet test; Comet assay; MTT assay; Cytotoxicity; DNA protection; Genotoxicity.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/946996
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Étude chez l'huître creuse, Crassostrea gigas, des anomalies génomiques provoquées par l'exposition à des concentrations environnementales de diuron : caractérisation des atteintes, étude de leur héritabilité et conséquences pour la survie et la croissance des naissains ArchiMer
Barranger, Audrey.
The aims of this PhD were to determine (1) the effects of the herbicide diuron on the genome of the oyster, Crassostrea gigas, (2) the possible transmission to the offspring of damaged DNA and (3) its consequences on oyster physiology. The genotoxicity of diuron and the vertical transmission of DNA damage were highlighted after parental exposure to environmental concentrations of diuron during the gametogenesis. Primary DNA lesions were detected in both somatic cells and germ cells of exposed genitors. Detection of the oxidized base 8-oxodGuo in gonads shows that exposure to diuron led to an oxidative stress which likely explains the observed DNA strand breaks in spermatozoa. The transmission of these DNA damage was confirmed by fluorescent in situ...
Tipo: Text Palavras-chave: Crassostrea gigas; Herbicide; Diuron; Génotoxicité; Effets trans-générationnels; Cytogénétique; Test des comètes; Cytométrie en flux; Crassostrea gigas; Herbicide; Diuron; Genotoxicity; Trans-generational effects; Cytogenetic; Comet assay; Flow cytometry.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00271/38237/36419.pdf
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Étude des voies de bioactivation du benzo[a]pyrène et du fluoranthène chez la sole commune (Solea solea) : Profil métabolique et génotoxicité ArchiMer
Wessel, Nathalie.
The aim of this Ph‟D was to study the biotransformation and the genotoxicity of two PAH of interest, namely the benzo[a]pyrene (BaP) and the fluoranthene (Fluo), in the common sole Solea solea, which is a benthic species sensitive to chemical pollution. The use of in vitro exposure models and the application of two different chemical analytical techniques allowed demonstrating that, as in higher vertebrates, the dihydrodiols and the phenols of BaP and Fluo are the major metabolites produced by sole liver enzymes. In vitro, the genotoxic potential of BaP and Fluo was demonstrated in sole, by the production of DNA adducts and oxidative DNA damage. The genotoxicity of Fluo appeared to be 50 fold less important than the genotoxicity of BaP. In the case of BaP,...
Tipo: Text Palavras-chave: Écotoxicologie; Hydrocarbures Aromatiques Polycycliques; Solea solea; Métabolisme; Génotoxicité; Ecotoxicology; Polycyclic Aromatic Hydrocarbons; Solea solea; Metabolism; Genotoxicity.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00032/14326/11609.pdf
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Evaluation of the genotoxicity of cellulose nanofibers Repositório Alice
LIMA, R. de; FEITOSA, L. O.; MARUYAMA, C. R.; BARGA, M. A.; YAMAWAKI, P. C.; VIEIRA, I. J.; TEIXEIRA, E. M.; CORRÊA, A. C; MATTOSO, L. H. C.; FRACETO, L. F..
bitstream/item/183092/1/Evaluation-of-the-genotoxicity-of-cellulose-nanofibers.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Nanotoxicology; Environmental nanotechnology; Curaua; Genotoxicity; Nanofibers; Cotton.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/940003
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Evaluation of the tickcide, genotoxic, and mutagenic effects of the Ruta graveolens L. (Rutaceae) Biological Sciences
Carvalho, Alessandra Vargas de; Lübeck, Irina; Rocha, Mariana Balhego da; Güez, Camila Martins; Machado, Michel Mansur; Oliveira, Luís Flávio Souza de.
Current analysis investigated the tickcide effects of the aqueous extract and chloroform fractions of Ruta graveolens L. (rue) on engorged females of Rhipicephalus microplus, as well as their genotoxic and mutagenic effects on human leukocytes. The best tickcide activity (non-dependent dose) and genotoxic / mutagenic effects (dependent-dose) were observed on exposure to chloroform fractions. Results suggest that extract fractions of R. graveolens L are efficient against R. microplus, although the fraction and the tested concentrations show genotoxic and mutagenic potential for human leukocytes. 
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.10.07.00-4 acaricide; Genotoxicity; Rue. Toxicologia e Etnofarmacologia.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/27576
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Genotoxic effect of high nitrate water consumption in men and woman from northern Mar del plata, Argentina JBAG
Poli,M.N; López Miranda,L.A; Fernández Iriarte,P.J; Zanier,G.J; Iúdica,C.E.
Previous research has shown connections between the exposure to different agents and mutations in germinal and somatic cells. One of such agents, nitrate (NO3), can be potentially genotoxic for individuals who drink high nitrate well water, and may cause a subsequent impact on their health and on the whole ecosystem. The aim of this research was to conduct genotoxicity assays in somatic cells from people living in the northern area of Mar del Plata city, Argentina. The purpose of these experiments was, in turn, to establish the possible relationships between mutagenic effects and high nitrate water consumption. To this end, different diagnostic tests were carried out to detect potential genetic and/or chromosomal alterations. A non-exposed population equal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mutagenic agents; Nitrate; Chromosomal aberrations; Genotoxicity; Micronuclei.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332012000100001
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Genotoxicity of diuron and glyphosate in oyster spermatozoa and embryos ArchiMer
Akcha, Farida; Spagnol, Charlene; Rouxel, Julien.
We investigated the effects of genotoxicant exposure in gametes and embryos to find a possible link between genotoxicity and reproduction/developmental impairment, and explore the impact of chemical genotoxicity on population dynamics. Our study focused on the genotoxic effects of two herbicides on oyster gametes and embryos: glyphosate (both as an active substance and in the Roundup formulation) and diuron. France is Europe's leading consumer of agrochemical substances and as such, contamination of France's coastal waters by pesticides is a major concern. Glyphosate and diuron are among the most frequently detected herbicides in oyster production areas; as oyster is a specie with external reproduction, its gametes and embryos are in direct contact with...
Tipo: Text Palavras-chave: Genotoxicity; Embryotoxicity; Oyster; Gametes; Pesticides; Diuron; Glyphosate.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00070/18128/15783.pdf
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Hematologic and hepatic responses of the freshwater fish Hoplias malabaricus after saxitoxin exposure. Repositório Alice
ASSIS, H. C. S. de; SILVA, C. A. da; OBA, E. T.; PAMPLONA, J. H.; MELA, M.; DORIA, H. B.; GUILOSKI, I. C.; RAMSDORF, W.; CESTARI, M. M..
The bioaccumulation of saxitoxins (STX) in the trophic chain, mainly in freshwater, are not completely known. This work aimed to elucidate the effects of STX on Hoplias malabaricus through trophic bioassay. The fish were fed once every five days with Astyanax sp. before being subjected to an intraperitoneal inoculation with the lysate of Cylindrospermopsis raciborskii culture containing 97% STX and 3% by neosaxitoxin and gonyautoxin during 20 days. The animal?s liver was assessed using biomarkers as activities of superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and glutathione peroxidase (GPx), and concentrations of reduced glutathione (GSH) and lipoperoxidation (LPO) and protein carbonylation (PCO). In the blood was analyzed...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Freshwater fish; Eutrophication; Cyanotoxin; Genotoxicidade; Genotoxicity; Paralytic shellfish poison; Peixe de água doce; Eutroficação; Cianotoxina.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/982178
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HEp-2 cell line as an experimental model to evaluate genotoxic effects of pentavalent inorganic arsenic JBAG
Andrioli,N.B; Chaufan,G; Coalova,I; Ríos de Molina,M.C; Mudry,M.D.
Early detection of toxic events induced by xenobiotics is necessary for a proper assessment of human risk after the exposure to those agents. The aim of this work was to evaluate the cell line HEp-2 as an experimental model to determine the genotoxic effects of sodium arsenate. To this end, we determined the metabolic activity cells by the MTT test on seven concentrations of arsenate that range from 27 to 135,000 &#956;M, obtaining the median lethal concentration (LC50), the lowest observed effect concentration (LOEC), and the not observed effect concentration (NOEC) of sodium arsenate at 24 h of exposition. According to the cytotoxic response obtained, we evaluated the genotoxic effect of the 27 and 270 &#956;M concentrations by using the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytotoxicity; Genotoxicity; Glutathione; HEp-2 cell line.
Ano: 2017 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332017000300002
Registros recuperados: 33
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