Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 30
Primeira ... 12 ... Última
Imagem não selecionada

Imprime registro no formato completo
Application of Oligonucleotide Microarray for the Detection and Genotyping of cry Genes in Bacillu thuringiensis Chilean J. Agric. Res.
Xu-Guang,Liu; Fu-Ping,Song; Si-Yuan,Wen; Sheng-Qi,Wang; Da-Fang,Huang; Jie,Zhang.
We have developed a parallel, rapid, high-throughput oligonucleotide microarray-based assay for the reliable detection and genotyping of three cry genes (cry1, cry2 and cry9) in Bacillus thuringiensis (Bt). After the non-polymerase chain reaction (PCR), amplified Bt genomic DNA were fluorescent-labeled using a random primer.   The corresponding oligonucleotide probes were designed for the different cry genes that can hybridize Bt genomic DNA after cluster analysis and were printed on glass slides. This microarray has unambiguously detected and identified the cry genes in 10 isolates and reference Bt. Our data demonstrates that the microarray assay is simple and rapid for the detection and genotyping of genes. This type of assay is also a potentially...
Tipo: Journal article Palavras-chave: Oligonucleotide microarray; Cry genes; Genotyping; Random primer labeling; Bioinformatics.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000200004
Imagem não selecionada

Imprime registro no formato completo
Avaliação genética das vacinas contra a brucelose bovina comercializadas no Brasil. Repositório Alice
BASTOS, R.; SOARES, C. O.; ELISEI, C.; MUNHOZ, A. L. R.; BEZERRA, N. L.; CAITANO, M. A. B.; ROSINHA, G. M. S..
Objetivou-se com este estudo caracterizar genotipicamente as amostras vacinais B19 e RB51 comercializadas no Brasil.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Brucella abortus; Brucellosis; Vaccine; B19; RB51; Genotyping; Genetic quality.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/937237
Imagem não selecionada

Imprime registro no formato completo
Characterization of mixed infections with different strains of bovine rotavirus in an outbreak of diarrhea in dairy herds in Goiás, Brazil BJM
Brito,Wilia Marta Elsner Diederichsen de; Munford,Veridiana; Villaça,André Martins; Caruzo,Thabata Alessandra Ramos; Rácz,Maria-Lucia.
Ten faecal samples of bovine rotavirus from calves less than 30 days old from an outbreak of diarrhea in Hidrolândia, Goiás, Brazil were submitted to serological and molecular characterization, using enzyme immunoassay for subgrouping and serotyping, PAGE for determination of electropherotypes and PCR for genome typing. Nine samples belonged to group A/subgroup I rotavirus and one sample was group A / subgroup non-I/non-II. Four samples were characterized as G10P[11] (B223-like), four samples showed a mixture of two rotavirus strains (G6G10 and P[5]P[11]), one sample was characterized as G6P[11] and one sample was characterized only by G serotyping/genotyping, and did not react with any P primer used. Two electropherotypes were detected and both were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rotavirus; Diarrhea; Bovine; Genotyping; Serotyping.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200015
Imagem não selecionada

Imprime registro no formato completo
Comparison of whole-genome amplifications for microsatellite genotyping of Rotylenchulus reniformis Electron. J. Biotechnol.
Arias,Renée S; Stetina,Salliana R; Scheffler,Brian E.
Currently, a large number of microsatellites are available for Rotylenchulus reniformis (reniform nematode); however, two barriers exist for genotyping samples from different geographical areas. The limited amount of nucleic acids obtained from single nematodes which would require their multiplication to obtain enough DNA for testing; and the strictly regulated transport of live samples and multiplication in greenhouse for being a plant pathogen. Whole-genome amplification (WGA) of samples consisting of one and five dead gravid females with their associated egg masses was successfully performed on disrupted tissue using three commercial kits. DNA yield after WGA ranged from 0.5 to 8 µg and was used to test 96 microsatellite markers we previously developed...
Tipo: Journal article Palavras-chave: Genotyping; Reniform nematode; SSR; STR; WGA.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300014
Imagem não selecionada

Imprime registro no formato completo
Compilation of an informative microsatellite set for genetic characterisation of East African finger millet (Eleusine coracana) Electron. J. Biotechnol.
De Villiers,Santie M; Michael,Vincent N; Manyasa,Eric O; Saiyiorri,Annis N; Deshpande,Santosh.
Background Genetic diversity of finger millet (Eleusine coracana), a nutritious neglected staple cereal in Africa and South Asia is largely uncharacterized. This study analysed 82 published SSR markers for finger millet across 10 diverse accessions to compile an informative set for genetic characterisation. Extensive optimization compared single samples with bulked leaf or bulked DNA samples for capturing within accession genetic diversity. The markers were evaluated to determine (1) how efficiently they amplified target loci during high-throughput genotyping with a generic PCR protocol, (2) ease of scoring PCR products and (3) polymorphism and ability to discern genetic diversity within the tested finger millet germplasm. Results Across 88 samples, the 52...
Tipo: Journal article Palavras-chave: Diversity analysis; SSR markers; Genotyping.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200003
Imagem não selecionada

Imprime registro no formato completo
DNA from tissues of young mice is optimal for genotyping Electron. J. Biotechnol.
Picazo,María G; García-Olmo,Dolores C.
Background Genotyping of mice is a common procedure in animal facilities. The aim of this study was to compare the quantity and quality of DNA extracted from samples obtained from young mice (YM; 10 d old) and adult mice (AM; 12 weeks old). We collected samples from the tail and ear of YM and AM. We also sampled blood, check cells (via buccal swabs), hair and fecal pellets of AM, and biopsied distal phalanx of YM. We isolated DNA using commercial kits and determined concentrations and purity by spectrophotometry. The integrity of DNA was evaluated by agarose-gel electrophoresis and polymerase chain reaction (PCR). Results DNA in all samples was amplified successfully but the intensities of bands after electrophoresis was heterogeneous. In general, tissues...
Tipo: Journal article Palavras-chave: DNA extraction; Genotyping; Mouse.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200004
Imagem não selecionada

Imprime registro no formato completo
Estrutura e diversidade genética de uma população de Coffea canephora conilon utilizando marcadores SNPs identificados por nextRAD. Repositório Alice
CARNEIRO, F. de A.; RÊGO, É. C. S.; AQUINO, S. O. de; COSTA, T. S.; LIMA, E. A. de; ROCHA, O. C.; RODRIGUES, G. C.; CARVALHO, M. A. de F.; MARRACCINI, P.; BARTHOLO, G. F.; GUERRA, A. F.; SILVA JUNIOR, O. B. da; GRATTAPAGLIA, D.; ANDRADE, A. C..
Este trabalho objetivou (i) avaliar e caracterizar, por meio da técnica de genotipagem nextRAD (Nextera-tagmented reductively-amplified DNA), a diversidade e a estrutura genética de indivíduos de C. canephora Conilon, pertencentes a uma população localizada em campo experimental da Embrapa Cerrados.
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Diversidade genética; Genotipagem nextRAD; Genetic diversity; Coffea canephora; Single nucleotide polymorphism; Genotyping; Genetic variation.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1029881
Imagem não selecionada

Imprime registro no formato completo
Genetic differences for behaviour in juveniles from two strains of brown trout suggest an effect of domestication history ArchiMer
Benhaim, David; Guyomard, Rene; Chatain, Beatrice; Quillet, Edwige; Begout, Marie-laure.
Because captivity constitutes a drastic environmental change, domestication is expected to induce a rapid genetic selection for behavioural traits. In this study, we searched for genetic differences in behaviour among brown trout juveniles from two strains differing for their domestication history, i.e. an almost pure native wild Mediterranean population (W) and an Atlantic domesticated strain (D). In order to assess pure genetic differences among strains, males from the two origins were mated with Mediterranean females to produce two experimental crosses (WW and WD). The swimming activity characteristics of individual WW and WD juveniles were compared before and after the application of a stress (light switched off suddenly, followed by a 5-min period of...
Tipo: Text Palavras-chave: Domestication; Genotyping; Microsatellite; Swimming activity; Restocking.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00152/26301/24409.pdf
Imagem não selecionada

Imprime registro no formato completo
Genetic differentiation between Cinta Senese and commercial pig breeds using microsatellite Electron. J. Biotechnol.
Scali,Monica; Vignani,Rita; Bigliazzi,Jacopo; Paolucci,Elisa; Bernini,Andrea; Spiga,Ottavia; Niccolai,Neri; Cresti,Mauro.
Background: Cinta Senese (CS) is an autochthonous Tuscan breed, which risked extinction since the ‘60s. Results: Monitoring the genetic variability of the actual population by use of DNA molecular markers is essential to address a correct breeding policy, finalized to obtain the race preservation and its fitness in the future. 17 SSRs autosomal markers and 1 associated to the X chromosome were used to genotype 86 individuals belonging to the CS and 12 belonging to two main white races Landrace (L), Large White (LW) and crosses between LW and L and L and CS widespread in Tuscany and used in the recent past to obtain hybrids with the CS. Conclusions: A dendrogram of similarity measures the relative genetic distance between individuals in the population. Data...
Tipo: Journal article Palavras-chave: Cinta Senese; Genetic diversity; Genotyping; Microsatellites; Sus scrofa.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200001
Imagem não selecionada

Imprime registro no formato completo
Genetic variability of Brazilian rice landraces determined by SSR markers. Repositório Alice
BORBA, T. C. de O.; MENDES, C. dos A.; GUIMARÃES, E. P.; BRUNES, T. O.; FONSECA, J. R.; BRONDANI, R. V.; BRONDANI, C..
Abstract ? The objective of this study was to evaluate the genetic variability of rice (Oryza sativa) landraces collected in Brazilian small farms. Twelve simple sequence repeat (SSR) markers characterized 417 landraces collected in 1986, 1987 and 2003, in the state of Goiás, Brazil. The number of landraces with long and thin grain type increased in the evaluated period, probably due to market demand. Based on the molecular data, the genetic variability increased during this period and, as per to the factorial correspondence analysis, most of the accessions were grouped according to the year of collection. The incorporation of modern rice cultivars in landrace cultivation areas and the selection carried out by small farmers are the most probable factors...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Genotyping; Participatory breeding; Rice breeding; Oriza sativa; Microsatellites.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/631815
Imagem não selecionada

Imprime registro no formato completo
Genome-wide copy number variation (CNV) detection in Nelore cattle reveals highly frequent variants in genome regions harboring QTLs affecting production traits. Repositório Alice
SILVA, J. M. da; GIACHETTO, P. F.; SILVA, L. O. da; CINTRA, L. C.; PAIVA, S. R.; YAMAGISHI, M. E. B.; CAETANO, A. R..
Background: Copy number variations (CNVs) have been shown to account for substantial portions of observed genomic variation and have been associated with qualitative and quantitative traits and the onset of disease in a number of species. Information from high-resolution studies to detect, characterize and estimate population-specific variant frequencies will facilitate the incorporation of CNVs in genomic studies to identify genes affecting traits of importance. Results: Genome-wide CNVs were detected in high-density single nucleotide polymorphism (SNP) genotyping data from 1,717 Nelore (Bos indicus) cattle, and in NGS data from eight key ancestral bulls. A total of 68,007 and 12,786 distinct CNVs were observed, respectively. Cross-comparisons of results...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bioinformática; Polimorfismo de nucleotídeo único; Genotipagem; Copy number variations; SNP genotyping; Gado de corte; Bioinformatics; Beef cattle; Single nucleotide polymorphism; High-throughput nucleotide sequencing; Genotyping.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1047683
Imagem não selecionada

Imprime registro no formato completo
Genomic structure and marker-derived gene networks for growth and meat quality traits of brazilian nelore beef cattle. Repositório Alice
MUDADU, M. de A.; PORTO NETO, L. R.; MOKRY, F. B.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; TULLIO, R. R.; NASSU, R. T.; NICIURA, S. C. M.; THOLON, P.; ALENCAR, M. M. de; HIGA, R. H.; ROSA, A. do N.; FEIJO, G. L. D.; FERRAZ, A. L. J.; SILVA, L. O. C. da; MEDEIROS, S. R. de; LANNA, D. P. D.; NASCIMENTO, M. L. do; CHAVES, A. S.; SOUZA, A. R. D. L.; PACKER, I. U.; TORRES JUNIOR, R. A. de A.; SIQUEIRA, F.; MOURAO, G. B.; COUTINHO, L. L.; REVERTER, A.; REGITANO, L. C. de A..
Nelore is the major beef cattle breed in Brazil with more than 130 million heads. Genome-wide association studies (GWAS) are often used to associate markers and genomic regions to growth and meat quality traits that can be used to assist selection programs. An alternative methodology to traditional GWAS that involves the construction of gene network interactions, derived from results of several GWAS is the AWM (Association Weight Matrices)/PCIT (Partial Correlation and Information Theory). With the aim of evaluating the genetic architecture of Brazilian Nelore cattle, we used high-density SNP genotyping data (~770,000 SNP) from 780 Nelore animals comprising 34 half-sibling families derived from highly disseminated and unrelated sires from across Brazil....
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: AWM; PCIT; GWAS; Genotyping.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1053714
Imagem não selecionada

Imprime registro no formato completo
Genomic structure and marker-derived gene networks for growth and meat quality traits of Brazilian Nelore beef cattle. Repositório Alice
MUDADU, M. de A.; PORTO-NETO, L. R.; MOKRY, F. B.; TIZIOTO, P. C.; OLIVEIRA, P. S. N.; TULLIO, R. R.; NASSU, R. T.; NICIURA, S. C. M.; THOLON, P.; ALENCAR, M. M. de; HIGA, R. H.; ROSA, A. do N.; FEIJO, G. L. D.; FERRAZ, A. L. J.; SILVA, L. O. C. da; MEDEIROS, S. R. de; LANNA, D. P.; NASCIMENTO, M. L.; CHAVES, A. S.; SOUZA, A. R. D. L.; PACKER, I. U.; TORRES JUNIOR, R. A. de A.; SIQUEIRA, F.; MOURÃO, G. B.; COUTINHO, L. L.; REVERTER, A.; REGITANO, L. C. de A..
Nelore is the major beef cattle breed in Brazil with more than 130 million heads. Genome-wide association studies (GWAS) are often used to associate markers and genomic regions to growth and meat quality traits that can be used to assist selection programs. An alternative methodology to traditional GWAS that involves the construction of gene network interactions, derived from results of several GWAS is the AWM (Association Weight Matrices)/PCIT (Partial Correlation and Information Theory). With the aim of evaluating the genetic architecture of Brazilian Nelore cattle, we used high-density SNP genotyping data (~770,000 SNP) from 780 Nelore animals comprising 34 half-sibling families derived from highly disseminated and unrelated sires from across Brazil....
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Genome-wide association studies; Association Weight Matrices; Partial Correlation and Information Theory; Gene; Genética; Gado Nelore; Gado de corte; Genótipo; Genotyping; Genotype; Nellore; Beef cattle; Genetic resources.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1059898
Imagem não selecionada

Imprime registro no formato completo
Genomic structure and marker-derived gene networks for growth and meat quality traits of Brazilian Nelore beef cattle. Repositório Alice
MUDADU, M. de A.; PORTO-NETO, L. R.; MOKRY, F. B.; TIZIOTO, P. C.; OLIVEIRA, P. S. N.; TULLIO, R. R.; NASSU, R. T.; NICIURA, S. C. M.; THOLON, P.; ALENCAR, M. M. de; HIGA, R. H.; ROSA, A. do N.; FEIJO, G. L. D.; FERRAZ, A. L. J.; SILVA, L. O. C. da; MEDEIROS, S. R. de; LANNA, D. P.; NASCIMENTO, M. L.; CHAVES, A. S.; SOUZA, A. R. D. L.; PACKER, I. U.; TORRES JUNIOR, R. A. de A.; SIQUEIRA, F.; MOURÃO, G. B.; COUTINHO, L. L.; REVERTER, A.; REGITANO, L. C. de A..
Nelore is the major beef cattle breed in Brazil with more than 130 million heads. Genome-wide association studies (GWAS) are often used to associate markers and genomic regions to growth and meat quality traits that can be used to assist selection programs. An alternative methodology to traditional GWAS that involves the construction of gene network interactions, derived from results of several GWAS is the AWM (Association Weight Matrices)/PCIT (Partial Correlation and Information Theory). With the aim of evaluating the genetic architecture of Brazilian Nelore cattle, we used high-density SNP genotyping data (~770,000 SNP) from 780 Nelore animals comprising 34 half-sibling families derived from highly disseminated and unrelated sires from across Brazil....
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Genome-wide association studies; Association Weight Matrices; Partial Correlation and Information Theory; Gene; Genética; Gado Nelore; Gado de corte; Genótipo; Genotyping; Genotype; Nellore; Beef cattle; Genetic resources.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1065945
Imagem não selecionada

Imprime registro no formato completo
Genomic variant hotspots in nelore cattle revealed by missing genotypes. Repositório Alice
GIACHETTO, P. F.; SILVA, J. M. da; SILVA, L. O. C. da; CINTRA, L. C.; PAIVA, S. R.; CAETANO, A. R.; YAMAGISHI, M. E. B..
In order to investigate whether or not the ?missing? genotypes actually reveal genomic variant hotspots, we examined BovineHD missing genotypes from a total of 1,709 Nelore DNA samples and used sequence data from eight of them to identify putative polymorphisms flanking the assayed SNPs.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Genotipagem; Polimorfismo de nucleotídeo único; Gado de corte; Single nucleotide polymorphism; Genotyping; Cattle.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1033991
Imagem não selecionada

Imprime registro no formato completo
Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie. Repositório Alice
GONÇALVES, A. N. D.; SOARES, C. O.; SANCHES, S. C.; REIS, F. A.; ROSINHA, G. M. S..
The objective of this work was to determine the genotypic profile specific to scrapie in codons 136, 154, and 171 of the PRNP gene of the Pantanal creole sheep. Genomic DNA was extracted from blood samples collected from 66 sheep, and the regions of interest on the DNA strand were amplified by PCR. Five haplotypes were identified: ARR, alanine, arginine, arginine; ARQ, alanine, arginine, glutamine; AHQ, alanine, histidine, glutamine; ARH, alanine, arginine, histidine; and VRQ, valine, arginine, glutamine. The most common genotypes were ARQ/ARQ (27%) and ARR/ARQ (24%). The genotypic profile of the Pantanal creole sheep shows low to moderate susceptibility.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Encefalopatia espongiforme transmissivel; Gene PRNP; Genotipagem; Polimorfismo de nucleotídeo único; Single nucleotide polymorphisms; Transmissible spongiform encephalopathies; Genotyping; Prion diseases.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1050554
Imagem não selecionada

Imprime registro no formato completo
High-density diversity arrays technology (DArT) genotyping for cost-effective mapping and genome-wide selection in Eucalyptus. Repositório Alice
SANSALONI, C. P.; PETROLI, C. D.; STEANE, D. A.; VAILLANCOURT, R. E.; CARLING, J.; MYBURG, A. A.; RESENDE, M. D. V. de; WENZL, P.; KILIAN, A.; GRATTAPAGLIA, D..
2009
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Eucalyptus; Genotyping.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/579665
Imagem não selecionada

Imprime registro no formato completo
High-throughput polymorphism detection and genotyping in polyploid Paspalum plicatulum using next-generation rad sequencing. Repositório Alice
OLIVEIRA, F. A. de; VIGNA, B. B. Z.; FAVERO, A. P.; HOHENLOHE, P. A.; SOUZA, A. P..
2015
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Pplymorphism detection; Genotyping; Paspalum plicatulum.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1034673
Imagem não selecionada

Imprime registro no formato completo
Identificação de novos acessos de Coffea Arábica introduzidos na coleção da Etiópia do IAPAR. Repositório Alice
FERREIRA, R. V.; ANDRADE, G. A.; CHARMETANT, P.; LEROY, T.; DOMINGUES, D. S.; PEREIRA, L. F. P..
Marcadores microssatélites foram utilizados na discriminação de novos acessos de Coffea arabica que foram introduzidos na coleção da Etiópia do IAPAR, provenientes de uma coleção similar na República de Camarões. Inicialmente 46 acessos de Camarões foram repassados para a Embrapa CPAC (Embrapa Cerrados ? Planaltina - DF), onde ficaram em quarentena. Em seguida, estes acessos foram transferidos para o IAPAR, porém durante os processos de quarentena e transferênc ia, alguns dos acessos foram misturados. Visando identificar corretamente os acessos e também validar os SSRs previamente identificados, todos os 46 acessos foram analisados em géis de poliacrilamida. Os 8 locos microssatélites utilizados geraram um total de 19 alelos, com uma média de 2,3 alelos...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Cafeeiro; Marcador microssatélite; Genotipagem; Coffee tree; Microsatellite markers; Café; Genotyping.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1040035
Imagem não selecionada

Imprime registro no formato completo
Identification of QTLs associated with biological nitrogen fixation traits in soybean using a genotyping-by-sequencing approach. Repositório Alice
GRUNVALD, A. K.; TORRES, A. R.; PASSIANOTTO, A. L. de L.; SANTOS, M. A.; JEAN, M.; BELZILE, F.; HUNGRIA, M..
bitstream/item/189995/1/Grunvald-2523.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Fixação de Nitrogênio; Soja; Soybeans; Nitrogen fixation; Genotyping.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103320
Registros recuperados: 30
Primeira ... 12 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional