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Activity of glucose-fructose oxidoreductase in fresh and permeabilised cells of Zymomonas mobilis grown in different glucose concentrations BJM
Erzinger,Gilmar Sidnei; Silveira,Mauricio Moura da; Costa,José Paulo Castilho Lopes da; Vitolo,Michele; Jonas,Rainer.
Previously grown cells of the ethanologenic bacterium Zymomonas mobilis produce sorbitol and gluconic acid, in reactions catalysed by the periplasmic enzymes glucose-fructose oxidoreductase (GFOR) and glucono-delta-lactonase (GL). The GFOR/GL system activity, in cells to be used in this bioconversion, depends on growth conditions. In batch runs, with initial glucose concentrations (S0) from 42 to 230 g/L, the highest specific and total GFOR/GL activities were obtained with S0 = 153 g/L (12.6 U/g cells and 62 U/L). Higher S0 led to decreasing activities in fresh cells. With S0 = 209 g/L, the final specific activity was only 7.0 U/g. After disruption of cells, however, an activity over 15 U/g was revealed. Since growth inhibition with S0 over 153 g/L was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zymomonas mobilis; Glucose-fructose oxidoreductase; Glucose.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400008
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Anesthesia of Epinephelus marginatus with essential oil of Aloysia polystachya: an approach on blood parameters Anais da ABC (AABC)
FOGLIARINI,CARINE O.; GARLET,QUELEN I.; PARODI,THAYLISE V.; BECKER,ALEXSSANDRO G.; GARCIA,LUCIANO O.; HEINZMANN,BERTA M.; PEREIRA,ANA MARIA S.; BALDISSEROTTO,BERNARDO.
ABSTRACT This study investigated the anesthetic potential of the essential oil (EO) of Aloysia polystachya in juveniles of dusky grouper (Epinephelus marginatus). Fish were exposed to different concentrations of EO of A. polystachya to evaluate time of induction and recovery from anesthesia. In the second experiment, fish were divided into four groups: control, ethanol and 50 or 300 µL L−1 EO of A. polystachya, and each group was submitted to induction for 3.5 min and recovery for 5 or 10 min. The blood gases and glucose levels showed alterations as a function of the recovery times, but Na+ and K+ levels did not show any alteration. In conclusion, the EO from leaves of A. polystachya is an effective anesthetic for dusky grouper, because anesthesia was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anesthetic efficacy; Blood gases; Glucose; Hemoglobin; Plasma ion levels.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200445
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Anesthetic activity of the essential oil of Ocimum americanum in Rhamdia quelen (Quoy & Gaimard, 1824) and its effects on stress parameters Neotropical Ichthyology
Silva,Lenise de Lima; Garlet,Quelen Iane; Koakoski,Gessi; Abreu,Murilo Sander de; Mallmann,Carlos Augusto; Baldisserotto,Bernardo; Barcellos,Leonardo José Gil; Heinzmann,Berta Maria.
ABSTRACT The aim of this study was to evaluate the anesthetic activity of the essential oil (EO) of Ocimum americanum L. in silver catfish (Rhamdia quelen ). In the first experiment, the depressor effects and chemical composition of the leaf EO (LEO) and inflorescence EO (IEO) were compared. Juveniles (n = 10) were placed in aquaria containing different concentrations of EO (25 - 500 mg L-1) to determine the point at which anesthesia was induced and the length of the recovery period. In the following experiment, the effects of 300 and 500 mg L-1 LEO exposure on stress parameters (plasma cortisol, glucose and sodium levels) after air exposure for 1 min were assayed. Fish (n = 10 per sampling time) were sampled immediately or transferred to anesthetic-free...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anesthesia; Basil; Cortisol; Glucose; Sodium.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000400715
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Approches cellulaires et moléculaires de la gestion des réserves chez l’huître, Crassostrea gigas (Thunberg, 1793). Suivi saisonnier du métabolisme énergétique et effet du niveau trophique sur la mise en réserve ArchiMer
Hanquet, Anne-caroline.
In the Pacific oyster, Crassostrea gigas, the energy necessary for general metabolism and gametogenesis is mainly provided by the glycogen stored in the vesicular cells. The first level of glycogen metabolism control is the entrance of glucose into cells. The monitoring of the uptake capacity of [U-14C] D-glucose revealed the existence of a saturable component with kinetic constants: Km = 0.71 mM ; Vmax = 0.22 nmol/h/106cells. The use of inhibitors has revealed the presence of carriers belonged to GLuT and SGLT types. A follow-up showed a seasonal variation of the uptake capacity and incorporation of glucose into glycogen, and a high expression of SGLT during mobilization of glycogen and gametes maturation. A bioassay in a controlled rearing system has...
Tipo: Text Palavras-chave: Crassostrea gigas; Glucides métabolisme; Glucose; Reproduction; Cryoconservation; Crassostrea gigas; Carbohydrate metabolism; Glucose; Reproduction; Cryoconservation.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00013/12399/9185.pdf
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Association between heart rate, heart rate variability, cortisol, glucose and electrolytes in healthy newborn calves Arq. Bras. Med. Vet. Zootec.
Quevedo,D.A.C.; Lourenço,M.L.G.; Bolaños,C.A.D.; Takahira,R.K.; Oba,E.; Alfonso,A.; Chiacchio,S.B..
ABSTRACT This study aims at evaluating the effects of electrolytes, glucose and cortisol levels over heart rate (HR) and heart rate variability (HRV) in healthy newborn calves. Seventeen healthy Holstein calves were evaluated during their first month of life, and the plasma concentrations of glucose, cortisol, calcium, magnesium, inorganic phosphorus, sodium and potassium were analyzed. HRV indexes were determined in the time and frequency domains through the analysis of neonatal electrocardiogram recordings. In the first day, low blood levels of phosphorus presented a strong correlation with the HR and the increased high-frequency components of HRV. The plasma concentrations of magnesium decreased significantly throughout the 35 days, revealing a positive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Calves; Electrolytes; Glucose; Cortisol; Heart rate variability.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000601922
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Avaliação dos efeitos da adição de sal e da densidade no transporte de tambaqui. Repositório Alice
GOMES, L. de C.; ARAÚJO-LIMA, C.A.R.M.; ROUBACH, R.; URBINATI, E.C..
Os objetivos deste trabalho foram testar a eficiência do sal como redutor de estresse e verificar a melhor densidade de transporte de juvenis de tambaqui (Colossoma macropomun) em caixas de plástico adaptadas. No primeiro experimento foram testadas diferentes concentrações de sal de cozinha (NaCl) na água; no segundo, o transporte foi realizado por três horas em caixas de plástico de 200 L estocadas com diferentes densidades de peixe, com 8 g de sal/L de água. O cortisol plasmático dos peixes sofreu aumento significativo após o transporte no tratamento sem sal e com 2 g de sal/L de água, retornando para níveis normais após 96 horas. A glicose plasmática dos peixes sofreu aumento após o transporte em todas as concentrações de sal testadas, com exceção da...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Colossoma macropomum; Glicose; Cloreto de sódio; Piscicultura; Glucose; Sodium chloride; Cortisol; Fish culture.
Ano: 2003 URL: http://www.alice.cnptia.embrapa.br/handle/doc/108909
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Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agents BABT
Rodrigues,Marcelo; Festucci-Buselli,Reginaldo Alves; Silva,Luzimar Campos; Otoni,Wagner Campos.
The use of cell and plant tissues culture techniques to produce economically important active metabolites has been growing. Among these substances, azadirachtin (AZA), produced by the neem tree (Azadirachta indica), has received considerable attention due to its bioinsecticide action. The main goal of this work was to analyze the AZA levels in neem cotyledonary calli. The calli were grown in agitated Woody Plant Medium (WPM) liquid medium, supplemented with glucose (Gl), hydrolyzed casein (HC) and methyl jasmonate (MeJ) as elicitor agent. An interaction was observed between these substances, depending on in vitro cultivation time with orbital agitation. The highest concentrations (average of 0.2470 µg g-1) of AZA were produced in the first and second weeks...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neem; HPLC; Glucose; Hydrolysed casein; Methyl jasmonate.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200001
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Azo dye degradation by Phanerochaete chrysosporium in the medium enriched with nitrogen in the presence of primary co-substrate BABT
Andrade,Marcus Vinicius Freire; Silva,Karla Mayara Lima da; Siqueira,João Paulo da Silva; Wanderley,Carlos Ronald Pessoa; Araújo,Rinaldo dos Santos; Marinho,Gloria; Rodrigues,Kelly.
This work sought to evaluate the ability of fungi Phanerochaete chryosporium to degrade congo red azo dye in batch reactor, evaluate the influence of glucose and wheat bran as co-substrates on the removal of the dye in the medium and investigate the influence of ammonium chloride, ammonium nitrate and ammonium sulfate as the inorganic nitrogen source for the process. Wheat bran was not effective satisfactorily for the removal of dye and organic matter had no desired effect for the removal of color and organic matter and showed the lowest values of k2, 0.008 M-1.d-1and 0.0004 M-1.d-1, respectively. Glucose presented the best response with the highest final percentage of dye removal (97%) and rate of dye removal (0.017 M-1.d-1), without adding an external...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wheat bran; Glucose; Congo red; Fungi; Nitrogen.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500019
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Biochemical and morphological changes during the growth kinetics of Araucaria angustifolia suspension cultures BABT
Santos,André Luis Wendt dos; Silveira,Vanildo; Steiner,Neusa; Maraschin,Marcelo; Guerra,Miguel Pedro.
Embryogenic cultures of Araucaria angustifolia were established in a BM liquid medium supplemented with 2 µM 2,4-dichlorophenoxyacetic acid, 1 µM 6-benzylaminopurine and 1 µM kinetin (BM2) and in a BM medium free of growth regulators (BM0). During 42 days in culture, the cell growth pattern of both cultures was similar. The pH of the culture medium of both BM0 and BM2 underwent progressive reduction during culture time. For both the embryogenic cultures a preferential uptake of glucose in the late stages of cell growth kinetics was observed. The extracellular protein content was similar for both the embryogenic cultures. Acetocarmine and Evan's blue double stain showed major differences for early somatic embryo organisation, in which only the embryogenic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Araucariaceae; Glucose; PH changes; Protein; Somatic embryogenesis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300001
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Biochemical changes in Salminus brasiliensis due to successive captures and stocking densities - doi: 10.4025/actascibiolsci.v35i1.13055 Biological Sciences
Braun, Neiva; Universidade Federal de Santa Catarina; Lima, Ronaldo Lima de; Universidade Federal de Santa Catarina; Baldisserotto, Bernardo; Universidade Federal de Santa Maria; Dafre, Alcir Luiz; Universidade Federal de Santa Catarina; Nuñer, Alex Pires de Oliveira; Universidade Federal de Santa Catarina.
This study evaluated the biochemical changes associated to successive captures of Salminus brasiliensis reared at different stocking densities. The experiment was conducted in tanks with a capacity of 1,000 L, connected to a recirculating system with temperature control and continuous aeration. Fish were stocked at densities of 30, 150 and 300 fish m-3 and the successive catches were applied at the end of the experimental period of 80 days. The biochemical changes were evaluated at the end of the trial through the parameters: hematocrit, hemoglobin, glucose and lactate in plasma and Na+, K+ and Cl- and Ca2+ in the gills. The concentration of glucose increased with the increase of stocking density as also registered for lactate concentration, except for...
Palavras-chave: Zoologia; Fisiologia; Bioquímica dourado; Hematocrit; Hemoglobin; Glucose; Lactate; Ions.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/13055
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Biochemical profile of amniotic fluid for the assessment of fetal and renal development BJMBR
Oliveira,F.R.; Barros,E.G.; Magalhães,J.A..
Creatinine plays a key role in the function and maturation of fetal kidneys throughout pregnancy. It is important to identify other markers that may help in the diagnosis of renal dysfunction. Our aim was to determine the profile of and the correlation between biochemical markers to be used to assess renal function and maturation of the fetus in the amniotic fluid during pregnancy and to determine the distribution of normal values for creatinine, N-acetyl-ß-D-glucosaminidase (NAG), ß2-microglobulin, glucose, urea, sodium, potassium, phosphorus, calcium, uric acid, albumin, and osmolality in three gestational age groups. This was a cross-section study that assessed 115 samples of amniotic fluid during three different periods of pregnancy, i.e., 13 to 20, 27...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fetal renal maturation; Normal pregnancy; Amniotic fluid; Creatinine; SS2-Microglobulin; Glucose; Uric acid.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200010
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Biosensor for determination of glucose in real samples of beverages Ciênc. Tecnol. Aliment.
Lopes,Flavio Marques; Batista,Karla de Aleluia; Batista,Gustavo Luiz Aleluia; Fernandes,Kátia Flávia.
A biosensor was developed for spectrophotometric determination of glucose concentrations in real samples of orange juice energetic drinks, and sport drinks. The biosensor consisted of glucose oxidase (GOD) and horseradish peroxidase (HRP) immobilized onto polyaniline activated with glutaraldehyde (PANIG). Immobilization parameters were optimized for GOD, and maximum immobilization yield was 16% when 5.0 mg of PANIG and 8.9 U prepared in 0.1 mol.L-1 sodium phosphate buffer (pH 7.0) reacted for 60 minutes at 4 °C with gentle stirring. The linear operational range for glucose determination using optimized operational parameters was between 0.05 and 6.0 mg.mL-1 with a very good reproducibility of response. The results obtained in the biosensor were compared...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosensor; Glucose; Glucose oxidase; Immobilization; Polyaniline.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100010
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Blood lactate threshold reflects glucose responses in horses submitted to incremental exercise test Arq. Bras. Med. Vet. Zootec.
Ferraz,G.C.; D’Angelis,F.H.F.; Teixeira-Neto,A.R.; Freitas,E.V.V.; Lacerda-Neto,J.C.; Queiroz-Neto,A..
Determinou-se, em eqüinos, o efeito do treinamento sobre as concentrações sangüíneas de lactato e plasmáticas de glicose durante exercício de intensidade progressiva em esteira rolante. Demonstrou-se que o treinamento aeróbico causou diminuição da concentração máxima de lactato e que o limiar de lactato corresponde ao ponto de inflexão da curva de glicose plasmática, confirmando esse parâmetro como indicador da capacidade aeróbica de cavalos.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Horse; Aerobic training; Lactate; Glucose; Performance.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000100035
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Blood metabolites as predictors to evaluate the body condition of Neopelma pallescens (Passeriformes: Pipridae) in northeastern Brazil Rev. Bras. Zool.
Azeredo,Luane M.M.; Oliveira,Talita C.; Lopez,Luiz C.S..
ABSTRACT Body condition is an important ecological attribute that can provide a measure of the energy stored by an animal or population. Body condition is traditionally ascertained with morphometric body condition indices (BCIs), but some researchers have used various physiological and biochemical parameters as predictors of body condition. This study aimed to test reliable biochemical predictors of body condition in individuals of Neopelma pallescens (Lafresnaye, 1853), found in an Atlantic forest patch. For this purpose we examined the relationship between body condition and the concentrations of blood glucose and ketones. We also verified correlations between blood metabolite concentrations and ecological variables, including time of day, seasons,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Birds; Ectoparasitism; Glucose; Incubation; Ketones.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702016000600901
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Carbohydrate measuvements on four brands of coconut water Ciência e Agrotecnologia
Oliveira,Hernete de Jesus Santos; Abreu,Celeste Maria Patto de; Santos,Custódio Donizete dos; Cardoso,Maria das Graças; Teixeira,Juliana Érica Carvalho; Guimarães,Nilson César Castanheira.
Utilization of water from dry coconuts to produce long-life coconut water, using a 20% mixture of green coconut water in order to reach the quality standard demanded by Brazilian customers, has been done since 2000. That is one more alternative for the by products from Cocos nucifera L. processing industries, introducing marketing advantages like: better storage, longer shelf life, transportation, and visual quality to the customer. Packed with ultrasonic Swedish technology, coconut water has been already commercialized in establishments in Southeastern Brazil. In this work, carbohydrate contents of four coconut water brands and the natural coconut water were comparatively analyzed, and the contents of fructose, glucose, sucrose, total sugars, and reducing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cocos nucifera L.; Carbohydrates; Fructose; Sucrose; Glucose.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542003000500013
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Carbohydrate metabolism and metabolic disorders in horses R. Bras. Zootec.
Hoffman,Rhonda M..
Horses evolved consuming primarily fermentable forage carbohydrates, but forage diets have been traditionally supplemented with grain meals rich in starch and sugar in order to provide additional calories, protein and micronutrients. Starch and sugar are important for performance horses, but the consumption starch-rich meals may cause equine digestive and metabolic disorders. The critical capacity for preileal starch digestibility appears to be 0.35 to 0.4% but may be as little, depending on the source of starch. Small intestinal absorption of simple sugars is limited by the activity and expression of two classes of glucose carrier proteins, which are affected by chronic intake of hydrolyzable carbohydrate but may be sluggish to respond to abrupt changes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbohydrate metabolism; Glucose; Horse; Insulin resistance.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009001300027
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Carbohydrates on the growth of Cattleya schilleriana Reichb.f. seedlings (Orchidaceae) Ciência Rural
Galdiano Júnior,Renato Fernandes; Mantovani,Cibele; Lemos,Eliana Gertrudes de Macedo.
ABSTRACT: The aim of the present study was to evaluate the effects of carbohydrate supplementation on the propagation of the orchid Cattleya schilleriana. The 120-d-old seedlings were subcultured in fructose-, glucose-, or sucrose-supplemented (0, 15, 30, and 45g L-1) ½ MS culture medium (half-strength macronutrient concentrations), using a completely random design with four repetitions per treatment. After 120d of treatment, root number and length, leaf number and length, and fresh weight were evaluated, and seedling survival was evaluated after 75d of acclimatization in a greenhouse. The in vitro growth data were submitted to regression analysis, whereas the percentage survival data were analyzed using ANOVA and Tukey’s test. Both in vitro growth and ex...
Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro culture; Native orchid; Glucose; Sucrose; Acclimatization.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700203
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Chemical pretreatment of Arundo donax L. for second-generation ethanol production. Repositório Alice
LEMÕES, J. S.; SILVA, C. F. L. e.; AVILA, S. P. F.; MONTERO, C. R. S.; SILVA, S. D. dos A. e; SAMIOS, D.; PERALBA, M. do C. R..
bitstream/item/187160/1/Sergio-Delmar-Chemical-pretreatment-of-Arundo-donax-L...r-second-generation.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Acid pretreatment; Alkaline pretreatment; Arundo biomass; Biofuel; Giant reed; Xylose Raw material; Enzymatic hydrolysis; Glucose; Lignocellulose; Response surface methodology.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100191
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Chickpea diseases resistance-screening techniques Open Agri
Y.L..
Palavras-chave: Chickpeas; Glucose; Distilling; Poultry equipment; Planting; Flours; Fungi; Disease resistance; Induced mutation; Water.
Ano: 1981 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4216
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Classification of Bovine Follicles Based on the Concentrations of Steroids, Glucose and Lactate in Follicular Fluid and the Status of Accompanying Follicles OAK
NISHIMOTO, Hiromi; HAMANO, Seizo; HILL, Glen Alan; MIYAMOTO, Akio; TETSUKA, Masafumi; 宮本, 明夫; 手塚, 雅文.
A simple and clear means to identify the physiological status of follicles is essential for study of follicular biology. In the present study, we verified a novel classification procedure based on analysis of the follicular population and glucose concentration in follicular fluid (FF) as an alternative method to classify bovine follicles. Paired ovaries were collected from heifers, and the number of follicles and stage of the CL were recorded. Follicles were initially divided into the following 3 groups according to diameter and the ratio of E2 and P4 (E/P): E2 active (E-A: E/P≥1), E2 inactive (E-I: E/P<1, ≥8.5 mm) and small follicles (E/P<1, <8.5 mm). E-A follicles were easily identified as E2-rich dominant follicles and were further classified...
Palavras-chave: Bovine; Follicular fluid; Follicle classification; Glucose; Lactate; Steroid.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2828
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