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A factorial design applied to the study of chromium toxicity on the glutathione levels of Brachiaria brizantha and Brachiaria ruziziensis seedlings. Repositório Alice
MARQUES, R.; OLIVEIRA, M. A. L. de; REIS, C. R. G. dos; KOPP, M. M.; PASSOS, L. P..
2015
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Brachiaria ruziziensis; Brachiaria brizantha; Glutathione; Chromium-toxicity; Oxidative stress.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1035418
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Altered Testicular Morphology and Oxidative Stress Induced by Cadmium in Experimental Rats and Protective Effect of Simultaneous Green Tea Extract International Journal of Morphology
El-Shahat,Abd El-Rahman; Gabr,Attia; Meki,Abdel-Raheim; Mehana,El-Saed.
Cadmium (Cd), is an environmental and industrial pollutant that affects the male reproductive system. Cd induces its effect by affecting tissue antioxidant enzyme systems. Green tea extract (GTE) is an antioxidant and free radicals scavenger and has a chelating property. The purpose of this study was to investigate the protective effect of GTE against testes damage induced by Cd. Four groups of male rats, were utilized as following: Controls, GTE treated, Cd treated and Cd + GTE, treated rats at the same doses. The rats received GTE and or Cd orally in drinking water. After 5 weeks, the animals were sacrificed and testes were removed for microscopic and Biochemical evaluation. The levels of lipid peroxides (LPO) and glutathione (GSH) were detected in the...
Tipo: Journal article Palavras-chave: Rat Testes; Cadmium toxicity; Green tea extract; Lipid peroxides; Glutathione.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000300020
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Antioxidant responses of cortex neurons to iron loading Biol. Res.
AGUIRRE,PABLA; MENA,NATALIA; TAPIA,VICTORIA; ROJAS,ALEJANDRO; ARREDONDO,MIGUEL; NÚÑEZ,MARCO TULIO.
Brain cells have a highly active oxidative metabolism, yet they contain only low to moderate superoxide dismutase and catalase activities. Thus, their antioxidant defenses rely mainly on cellular reduced glutathione levels. In this work, in cortical neurons we characterized viability and changes in reduced and oxidized glutathione levels in response to a protocol of iron accumulation. We found that massive death occurred after 2 days in culture with 10 mM Fe. Surviving cells developed an adaptative response that included increased synthesis of GSH and the maintenance of a glutathione-based reduction potential. These results highlight the fundamental role of glutathione homeostasis in the antioxidant response and provide novel insights into the adaptative...
Tipo: Journal article Palavras-chave: Glutathione; Iron; Redox response; Cortical neurons; Cell death.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100012
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Determination of reduced sulfur compounds by high-performance liquid chromatography in hydrothermal seawater and body fluids from Riftia pachyptila ArchiMer
Gru, Cecile; Sarradin, Pierre-marie; Legoff, Hélène; Narcon, Sandrine; Caprais, Jean-claude; Lallier, Francois.
This paper describes a method for the determination of reduced sulfur compounds in hydrothermal seawater and body fluids from the hydrothermal tube worm Riftia pachyptila. Sulfur is a hey component of the hydrothermal ecosystem based on chemosynthesis, Sulfur compounds were derivatized at pH 8 (4.3 for sulfide in biological fluid) with a fluorescent reagent, monobromobimane, and separated by reverse-phase HPLC. The eluted compounds were detected by spectrofluorimetry. This method allowed: the quantitative analysis of sulfide, sulfite, thiosulfate, cysteine and glutathione in seawater, vascular blood and coelomic fluids from R, pachyptila, The detection limits were in the 0.1 mu mol l(-1) range with a precision lower than 10%, The method has been applied to...
Tipo: Text Palavras-chave: Sulfide; Sulfite; Thiosulfate; Cysteine; Glutathione; Hydrothermal seawater; Riftia pachyptila; Biological fluids.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00186/29763/28203.pdf
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Effect of fish meal replacement by plant protein sources on non-specific defence mechanisms and oxidative stress in gilthead sea bream (Sparus aurata) ArchiMer
Sitja Bobadilla, A; Pena Llopis, S; Gomez Requeni, P; Medale, Francoise; Kaushik, Sadasivam; Perez Sanchez, J.
Partial or total replacement of fish meal by a mixture of plant protein (PP) sources (corn gluten, wheat gluten, extruded peas, rapeseed meal and sweet white lupin) balanced with indispensable amino acids was examined in juvenile gilthead sea bream over the course of a 6-month growth trial. A diet with fish meal (FM) as the sole protein source was compared to diets with 50%, 75% and 100% of replacement (PP50, PP75, and PP100). The possible influence of diets on growth performance, plasma metabolites, gut integrity, liver structure, anti-oxidant and immune status was evaluated. Final body weight was progressively decreased with PP inclusion, but in PP50 and PP75-fed fish, feed efficiency (FE) was significantly improved and specific growth rates remained...
Tipo: Text Palavras-chave: Gilthead sea bream; Cholesterol; Glutathione; Histopathology; Myeloperoxidase; Complement; Lysozyme; Respiratory burst; Immune response; Growth; Plant proteins; Fish meal.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-3595.pdf
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Effects of buthionine sulfoximine nifurtimox and benznidazole upon trypanothione and metallothionein proteins in Trypanosoma cruzi. Biol. Res.
MAYA,JUAN DIEGO; RODRÍGUEZ,ANDRÉS; PINO,LAURA; PABÓN,ADRIANA; FERREIRA,JORGE; PAVANI,MARIO; REPETTO,YOLANDA; MORELLO,ANTONIO.
Proteins rich in sulfhydryl groups, such as metallothionein, are present in several strains of the parasite Trypanosoma cruzi, the etiological agent of Chagas' disease. Metallothionein-like protein concentrations ranged from 5.1 to 13.2 pmol/mg protein depending on the parasite strain and growth phase. Nifurtimox and benznidazole, used in the treatment of Chagas' disease, decreased metallothionein activity by approximately 70%. T. cruzi metallothionein was induced by ZnCl2. Metallothionein from T. cruzi was partially purified and its monobromobimane derivative showed a molecular weight of approximately 10,000 Da by SDS-PAGE analysis. The concentration of trypanothione, the major glutathione conjugate in T. cruzi, ranged from 3.8 to 10.8 nmol/mg protein,...
Tipo: Journal article Palavras-chave: Trypanosoma cruzi; Metallothionein; Glutathione; Trypanothione; Benznidazole; Nifurtimox; Buthionine sulfoximine.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100007
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Elicitation of peroxidase activity in genetically transformed root cultures of Beta vulgaris L. Electron. J. Biotechnol.
Rudrappa,Thimmaraju; Neelwarne,Bhagyalakshmi; Lakshmanan,Venkatachalam; Reddampalli Venkataramareddy,Sreedhar; Gokare Aswathanarayana,Ravishankar.
Genetically transformed roots of red beet produce copious levels of peroxidase (POD) - a multifunctional enzyme with a number of commercial applications. In an effort to elicit the POD activity, the cultures were treated with biotic elicitors such as dry cell powders of microbial cultures (0.1-0.5% w/v) and the respective culture filtrates (1-5% v/v). Similarly, abiotic elicitors, particularly metal ions (2-8 folds of that present in the nutrient medium), the plant hormone Thidiazuron (at 0.25-1 ppm) and other bio-molecules such as Glutathione (at 0.5-10 mM) and Methyl jasmonate (at 20-100 µM) were used. It was observed that dry cell powder of Candida versatilis significantly elicited the enzyme activity (3.52-fold higher than the control) followed by...
Tipo: Journal article Palavras-chave: Aspergillus; Calcium; Culture filtrate; Dried cell powder; Elicitor; Glutathione; Methyljasmonate; Rhizophus; Thidiazuron.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500006
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HEp-2 cell line as an experimental model to evaluate genotoxic effects of pentavalent inorganic arsenic JBAG
Andrioli,N.B; Chaufan,G; Coalova,I; Ríos de Molina,M.C; Mudry,M.D.
Early detection of toxic events induced by xenobiotics is necessary for a proper assessment of human risk after the exposure to those agents. The aim of this work was to evaluate the cell line HEp-2 as an experimental model to determine the genotoxic effects of sodium arsenate. To this end, we determined the metabolic activity cells by the MTT test on seven concentrations of arsenate that range from 27 to 135,000 μM, obtaining the median lethal concentration (LC50), the lowest observed effect concentration (LOEC), and the not observed effect concentration (NOEC) of sodium arsenate at 24 h of exposition. According to the cytotoxic response obtained, we evaluated the genotoxic effect of the 27 and 270 μM concentrations by using the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytotoxicity; Genotoxicity; Glutathione; HEp-2 cell line.
Ano: 2017 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332017000300002
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Selenium invoked antioxidant defense system in Azolla caroliniana plant Phyton
Hassan,AMA; Mostafa,EM.
Plants of Azolla caroliniana were treated with different selenium concentrations (0, 1, 2, 5, 7, 10 ppm) for seven days. Selenium (Se) content in Azolla plants increased significantly with increasing Se concentrations in the culture media up to 5 ppm. This indicated that Azolla plants were a good accumulator for Se. Selenium accumulation determined changes in Azolla biomass, doubling time and relative growth rates. Treatment of Azolla plants with low concentrations of Se (1 ppm) resulted in a significant increase in biomass. This was accompanied by a reduction in hydrogen peroxide and malondialdehyde (MDA) contents; the decrease percentages were 78% and 60%, respectively at 1 ppm Se in comparison with the control. At higher Se concentrations (>5 ppm),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Selenium; Azolla caroliniana; Ascorbate; Glutathione; Antioxidant enzymes; Glutathione peroxidase.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000200014
Registros recuperados: 9
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