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Agrobacterium rhizogenes mediated genetic transformation resulting in hairy root formation is enhanced by ultrasonication and acetosyringone treatment Electron. J. Biotechnol.
Kumar,Vinod; Sharma,Ashwani; Narasimha Prasad,Bellur Chayapathy; Bhaskar Gururaj,Harishchandra; Aswathanarayana Ravishankar,Gokare.
The phytopathogenic bacteria Agrobacterium rhizogenes genetically transforms plants by transferring a portion of the resident Ri plasmid, the T-DNA to the plant. Plant species differ in their temporal competence for transformation. But various physical and chemical methods are found to enhance transformation frequency. Agrobacterium rhizogenes mediated transformation efficiency was assessed under the influence of sonication, calcium treatment, acetosyringone and macerating enzymes in suitable combinations in Nicotiana tabacum as a model system. Manual wounding resulted in 21% transformation frequency. Where as sonication resulted in 2.2 fold increase, followed by sonication with CaCl2 treatment resulted in 2.5 fold increase and sonication with...
Tipo: Journal article Palavras-chave: Agrobacterium rhizogenes; Hairy roots; Nicotiana tabacum; Transformation frequency; Ultra-sonication.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400002
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Agrobacterium rhizogenes vs auxinic induction for in vitro rhizogenesis of Prosopis chilensis and Nothofagus alpina Biocell
Caro,Luis A.; Santecchia,Natalia; Marinangeli,Pablo A.; Curvetto,Néstor R.; Hernández,Luis F..
The induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (BTMm) containing half salt concentration of macronutrients and 0.05 mg.L-1 benzilaminopurine (BAP). After 30 days, microshoots with 2-4 leaves were selected and cultured in BTMm-agar in presence or abscense of Ar and in combination with IBA. For N. alpina, the apical shoots with the first 2 true leaves, from 5 weeks old seedlings, were cultured in the abovementioned...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agrobacterium rhizogenes; Algarrobo; Hairy roots; Indole-3-butyric acid; Nothofagus nervosa; Prosopis chilensis; Raulí; Rooting.
Ano: 2003 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300002
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Analysis and sequencing of h6hmRNA, last enzyme in the tropane alkaloids pathway from anthers and hairy root cultures of Brugmansia candida (Solanaceae) Electron. J. Biotechnol.
Cardillo,Alejandra B.; Giulietti,Ana M.; Marconi,Patricia L..
Brugmansia candida (Solanaceae) is a native tree distributed across South-American and produces the pharmacologically- important group of tropane alkaloids including scopolamine. This biocompound is synthesised from hyoscyamine by action of Hyoscyamine 6-β hydroxylase (H6H, EC 1.14.11.11) at the end of the tropane alkaloid pathway. Here are reported the tissue and organ-specific expression of h6hmRNA by RT-PCR analyses and the isolation, cloning and sequencing of the cDNA obtained from B. candida anthers and hairy root transformed cultures. Bioinformatic analysis of the nucleotide sequence revealed an uninterrupted ORF of 1038 bp and the predicted aminoacid sequence could be 344 aminoacid long. A database search showed that this sequence has high...
Tipo: Journal article Palavras-chave: H6H; Hairy roots; Hyoscyamine; Scopolamine; Sequence analysis; Transformation.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300004
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Efficient genetic transformation and regeneration system from hairy root of Origanum vulgare. Repositório Alice
HABIBI, P.; SA, M. F. G. de; SILVA, A. L. L. da; MAKHZOUM, A.; COSTA, J. da L.; BORGHETTI, I. A.; SOCCOL, C. R..
bitstream/item/148212/1/art-10.1007-s12298-016-0354-2.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Agrobacterium rhizogenes; Hairy roots; Calli; Regeneration; Origanum vulgare.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1053999
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