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Registros recuperados: 26
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A la recherche de l'huître portuguaise: identification de marqueurs moléculaires discriminant Crassostrea angulata et Crassostrea gigas ArchiMer
Cornette, Florence.
L'étude historique de l'ostréiculture française monte que cette activité est caractérisée par des périodes de prospérités et de pénurie. Des épizooties ont été à l'origine de ces alternances. A chacune de ces période, on a pu constater des changements d'espèces : Ostrea edulis fut remplacée par Crassostrea angulata, de même celle-ci fut remplacée par Crassostrea gigas dans les années 1970. L'étude de la bibliographie montre que les techniques utilisées pour distinguer des espèces Crassostrea angulata et Crassostrea gigas sont insuffisantes. L'évolution des technologies en matière d'analyses génétiques ouvrent de nouvelles perspectives. La recherche de marqueurs moléculaires réalisée sur Crassostrea angulata et Crassostrea gigas, par amplification par PCR...
Tipo: Text Palavras-chave: Crassostrea angulata; Crassostrea gigas; Marqueurs moléculaires; ADN mitochondrial; PCR; Enzymes de restrictions; Haplotypes.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00049/15979/13421.pdf
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alpha-thalassemia, HbS, and beta-globin gene cluster haplotypes in two Afro-Uruguayan sub-populations from northern and southern Uruguay Genet. Mol. Biol.
Luz,Julio A. da; Sans,Mónica; Kimura,Elza Miyuki; Albuquerque,Dulcinéia Martins; Sonati,Maria de Fatima; Costa,Fernando Ferreira.
Hemoglobinopathies are the most common monogenic disorders worldwide; however, they have never been systematically studied from a genetic perspective in Uruguay. In this study, we determined the frequencies of hemoglobin variants in Afro-Uruguayans. A sample of 52 healthy unrelated Afro-Uruguayans from the northern (N = 28) and southern (N = 24) regions of the country was analyzed. Eight individuals (15.4%) were heterozygous for -alpha3,7thalassemia; seven of them (29.2%) were originally from the southern region, whereas one of them (3.6%) was from the northern region; the differences between both regions were statistically significant (p = 0.016 +/-0.003). The only structural mutation detected was betaS, which is typical of African populations. Four...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hemoglobinopathies; Alpha-thalassemias; HbS; Haplotypes; Afro-derived populations.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400002
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An insight into the linkage disequilibrium map of the Canchim beef cattle breed. Repositório Alice
MOKRY, F. B.; LIMA, A. O. de; MUDADU, M. A.; HIGA, R. H.; MEIRELLES, S. L. C.; REGITANO, L. C. de A..
The development of linkage disequilibrium (LD) maps is very important for understanding the nature of non-linear association between phenotypes and genes, as LD can be defined as the non-random segregation of a pair of alleles at polymorphic sites. Canchim is a composite beef cattle breed which was developed in the 1960's by the crossing of Bos indicus (3/8) x Charolais (5/8) animals. The objective in this study was to analyze the extension of LD maps from a Canchim beef cattle population.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Desequilíbrio de ligação; Gado; Linkage disequilibrium; Cattle; Zebu; Haplotypes.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/946432
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Association analyses pointed the TIPARP as a potential candidate gene influencing residual feed intake variation in Nelore cattle. Repositório Alice
LIMA, A. O. de; OLIVEIRA, P. S. N. de; TIZIOTO, P. C.; AFONSO, J.; SOMAVILLA, A. L.; DINIZ, W. J. da S.; SILVA, J. V. da; ROCHA, M. I. P.; MUDADU, M. de A.; COUTINHO, L. L.; REGITANO, L. C. de A..
2016
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Haplótipo; SNPs; Gado de corte; Gado nelore; Haplotypes; Nellore.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1056239
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Beta-globin gene cluster haplotypes in Venezuelan sickle cell patients from the State of Aragua Genet. Mol. Biol.
Moreno,Nancy; Martínez,José A.; Blanco,Zorella; Osorio,Leidys; Hackshaw,Patrick.
Seven polymorphic sites in the beta-globin gene cluster were analyzed on a sample of 96 chromosomes of Venezuelan sickle cell patients from the State of Aragua. The Benin haplotype was predominant with a frequency of 0.479, followed by the Bantu haplotype (0.406); a minority of cases with other haplotypes was also identified: atypical Bantu A2 (0.042), Senegal (0.031), atypical Bantu A7 (0.021) and Saudi Arabia/Indian (0.021) haplotypes; however, the Cameroon haplotype was not identified in this study. Our results are in agreement with the historical records that establish Sudanese and Bantu origins for the African slaves brought into Venezuela.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Haplotypes; Beta-globin gene cluster; Sickle cell.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100005
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CYP1A1 genotypes and haplotypes and risk of oral cancer: a case-control study in South Indians Genet. Mol. Biol.
Balaji,Lakshmi; Singh,Krishna Balaji; Bhaskar,Lakkakula V.K.S..
The CYP1A1 gene encodes for the enzyme, aryl hydrocarbon hydroxylase, which is involved in the biotransformation of various aromatic tobacco precarcinogens. In the present study, the association between CYP1A1 gene polymorphisms (IVS1-728G > A, Thr461Asn and Ile462Val), and the risk of oral cancer, was examined among 157 patients with oral cancer and 132 age-matched controls, in a south Indian population. The strength of the association between CYP1A1 variants and oral cancer was estimated by logistic regression. It was found that Thr461Asn was not polymorphic. Both IVS1-728G > A and Ile462Val frequencies were consistent with Hardy-Weinberg equilibrium in the control group. There were no significant differences in genotype or haplotype frequencies...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CYP1A1; Haplotypes; Allele; Oral cancer.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000300004
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Descriptive analysis of haplotypes in a population of Canchim beef cattle. Repositório Alice
MOKRY, F. B.; LIMA, A. O.; MUDADU, M. de A.; HIGA, R. H.; MEIRELLES, S. L. C.; SILVA, M. V. G. B.; CARDOSO, F. F.; NICIURA, S. C. M..
2012
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Haplotypes; Beef cattle; Race Canchim.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/946401
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Determination of βS haplotypes in patients with sickle-cell anemia in the state of Rio Grande do Norte, Brazil Genet. Mol. Biol.
Cabral,Cynthia Hatsue Kitayama; Serafim,Édvis Santos Soares; Medeiros,Waleska Rayane Dantas Bezerra de; Fernandes,Thales Allyrio Araújo de Medeiros; Kimura,Elza Miyuki; Costa,Fernando Ferreira; Sonati,Maria de Fátima; Rebecchi,Ivanise Marina Moretti; Medeiros,Tereza Maria Dantas de.
βS haplotypes were studied in 47 non-related patients with sickle-cell anemia from the state of Rio Grande do Norte, Brazil. Molecular analysis was conducted by PCR/RFLP using restriction endonucleases XmnI, HindIII, HincII and HinfI to analyze six polymorphic sites from the beta cluster. Twenty-seven patients (57.5%) were identified with genotype CAR/CAR, 9 (19.1%) CAR/BEN, 6 (12.8%) CAR/CAM, 1 (2.1%) BEN/BEN, 2 (4.3%) CAR/Atp, 1 (2.1%) BEN/Atp and 1 (2.1%) with genotype Atp/Atp. The greater frequency of Cameroon haplotypes compared to other Brazilian states suggests the existence of a peculiarity of African origin in the state of Rio Grande do Norte.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Haplotypes; Β-globin; Sickle-cell anemia; Brazilian population; S hemoglobin.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300009
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Differential fine-tuning of gene expression regulation in coffee leaves by CcDREB1D promoter haplotypes under water deficit. Repositório Alice
ALVES, G. S. C.; TORRES, L. F.; DÉCHAMP, E.; BREITLER, J-C.; JOËT, T.; GATINEAU, F.; ANDRADE, A. C.; BERTRAND, B.; MARRACCINI, P.; ETIENNE, H..
Despite the importance of the DREB1D gene (also known as CBF4) in plant responses to water deficit and cold stress, studies analysing its regulation by transgenic approaches are lacking. In the current work, a functional study of three CcDREB1D promoter haplotypes (named HP15, HP16 and HP17) isolated from drought-tolerant and droughtsensitive clones of Coffea canephora was carried out in plants of C. arabica stably transformed by Agrobacterium tumefaciens by analysing their ability to regulate the expression of the uidA reporter gene in response to water deficit mimicked by polyethylene glycol (−2.0 MPa) and low relative humidity treatments.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Coffe; Water deficit; Genetic transformation; Haplotypes; Reporter gene.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1084513
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Evaluating methodologies for species delimitation: the mismatch between phenotypes and genotypes in lichenized fungi (Bryoria sect. Implexae, Parmeliaceae) Naturalis
Boluda, C.G.; Rico, V.J.; Divakar, P.K.; Nadyeina, O.; Myllys, L.; McMullin, R.T.; Zamora, J.C.; Scheidegger, C.; Hawksworth, D.L..
In many lichen-forming fungi, molecular phylogenetic analyses lead to the discovery of cryptic species within traditional morphospecies. However, in some cases, molecular sequence data also questions the separation of phenotypically characterised species. Here we apply an integrative taxonomy approach ‒ including morphological, chemical, molecular, and distributional characters ‒ to re-assess species boundaries in a traditionally speciose group of hair lichens, Bryoria sect. Implexae. We sampled multilocus sequence and microsatellite data from 142 specimens from a broad intercontinental distribution. Molecular data included DNA sequences of the standard fungal markers ITS, IGS, GAPDH, two newly tested loci (FRBi15 and FRBi16), and SSR frequencies from 18...
Tipo: Article / Letter to the editor Palavras-chave: Chemotypes; Cryptic species; Haplotypes; Incomplete lineage sorting; Integrative taxonomy; Microsatellites; Speciation; Species concepts.
Ano: 2019 URL: http://www.repository.naturalis.nl/record/660277
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Genetic association of SNPs in the FTO gene and predisposition to obesity in Malaysian Malays BJMBR
Apalasamy,Y.D.; Ming,M.F.; Rampal,S.; Bulgiba,A.; Mohamed,Z..
The common variants in the fat mass- and obesity-associated (FTO) gene have been previously found to be associated with obesity in various adult populations. The objective of the present study was to investigate whether the single nucleotide polymorphisms (SNPs) and linkage disequilibrium (LD) blocks in various regions of the FTO gene are associated with predisposition to obesity in Malaysian Malays. Thirty-one FTO SNPs were genotyped in 587 (158 obese and 429 non-obese) Malaysian Malay subjects. Obesity traits and lipid profiles were measured and single-marker association testing, LD testing, and haplotype association analysis were performed. LD analysis of the FTO SNPs revealed the presence of 57 regions with complete LD (D’ = 1.0). In addition, we...
Tipo: Info:eu-repo/semantics/article Palavras-chave: FTO gene; SNP; Obesity; Linkage disequilibrium; Haplotypes.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200003
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Genetic structure of the ornamental tetra fish species Piabucus melanostomus Holmberg, 1891 (CHARACIDAE, IGUANODECTINAE) in the Brazilian Pantanal wetlands inferred by mitochondrial DNA sequences Biota Neotropica
Borba,Rafael Splendore de; Silva,Edson Lourenço da; Ponzetto,Josi Margarete; Pozzobon,Allan Pierre Bonetti; Centofante,Liano; Alves,Anderson Luis; Parise-Maltempi,Patrícia Pasquali.
The subfamily Iguanodectinae comprises a group of small Neotropical fishes composed by two genera and 11 nominal species widely distributed in the Atlantic drainages of South America. Piabucus is the only genus of Iguanodectinae found in the Paraguay River basin, especially in the Pantanal of Mato Grosso State, where it is represented by Piabucus melanostomus. Given the wide distribution and the low dispersion capacity of this species, due the ecological constraints, it is possible that many interesting genetic features could be found in different populations. In this way, the aim of his work was to perform the phylogeographic pattern of P. melanostomus populations using mitochondrial DNA sequences. A total of 13 individuals from three rivers belonging the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Flood pulse; Genetic diversity; Gene flow; Haplotypes.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032013000100004
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Haplotypes of the bovine IgG2 heavy gamma chain in tick-resistant and tick-susceptible breeds of cattle. Repositório Alice
CARVALHO, W. A.; IANELLA, P.; ARNOLDI, F. G. C.; CAETANO, A. R.; MARUYAMA, S. R.; FERREIRA, B. R.; CONTI, L. H. A.; SILVA, M. R. M. da; PAULA, J. O. F.; MAIA, A. A. M.; SANTOS, I. K. F. de M..
bitstream/item/183396/1/Carvalho2011-Article-HaplotypesOfTheBovineIgG2Heavy.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bovine IgG2; Allotypes; Hinge region; Rhipicephalus (Boophilus) microplus; Haplotypes.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/902013
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Low mtDNA diversity in a highly differentiated population of spinner dolphins (Stenella longirostris) from the Fernando de Noronha Archipelago, Brazil. Repositório Alice
FARIA, D. M.; SILVA JÚNIOR, J. M. da; COSTA, L. P.; PAIVA, S. R.; MARINO, C. L.; ROLLO JÚNIOR, M. M.; BAKER, C. S.; FARRO, A. P. C..
bitstream/item/213180/1/journal.pone.0230660.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Stenella longirostris; DNA sequencing; Archipelagoes; Brazil; Dolphins; Mitochondrial DNA; Haplotypes.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1122499
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Mitochondrial markers to distinguish Spodoptera frugiperda populations associated with corn and cotton crops PAB
Queiroz,Paulo Roberto; Ramiro,Carolina Almeida; Martins,Érica Soares; Soberón,Mário; Bravo,Alejandra; Monnerat,Rose Gomes.
Abstract: The objective of this work was to analyze the genetic variability of Spodoptera frugiperda (Lepidoptera: Noctuidae) populations collected from corn and cotton crops in Brazil. The samples were analyzed by DNA markers. The dendrogram produced by the 20 RAPD primers evaluated showed a correlation between genetic profile and feeding behavior. The analysis of the mitochondrial ND1 gene allowed identifying the insect populations in both crops, and, in corn, in several geographical regions. The presented strategy allows the identification of Spodoptera frugiperda populations associated with corn and cotton crops.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gossypium hirsutum; Zea mays; Fall armyworm; Haplotypes; ND1 gene; RAPD..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500692
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Modulation of the prostaglandin-endoperoxide synthase 2 gene expression by variant haplotypes: influence of the 3′-untranslated region BJMBR
Piranda,D.N.; Abreu,R.B.V.; Freitas-Alves,D.R.; de Carvalho,M.A.; Vianna-Jorge,R..
The inducible inflammatory enzyme cycloxigenase-2 is up-regulated in cancer, and favors tumor progression. Cycloxigenase-2 is encoded by the prostaglandin-endoperoxide synthase 2 (PTGS2) gene, which presents sequence variations in the promoter region (PR) and in the 3′-untranslated region (3′-UTR). Different PR (rs689465, rs689466, rs20417) and 3′-UTR (rs5275) variants were generated by site-directed mutagenesis, and combined in haplotypes to access expression levels using a reporter system (luciferase) in human cells (MCF-7 and HEK293FT). Luciferase activity did not differ significantly among PTGS2 PR constructs, except for pAAC (containing variant allele rs20417 C), with 40% less activity than pAAG (wild-type sequence) in MCF-7 cells (P<0.01). Despite...
Tipo: Info:eu-repo/semantics/article Palavras-chave: COX-2; PTGS2; Haplotypes; Gene expression; MCF-7; HEK293FT.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200602
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Molecular characterization of Aedes aegypti (L.) (Diptera: Culicidae) of Easter Island based on analysis of the mitochondrial ND4 gene Rev. Bras. entomol.
Núñez,Claudia Andrea; González,Christian Raúl; Obreque,Víctor; Riquelme,Brenda; Reyes,Carolina; Rojas,Mabel.
ABSTRACT Aedes aegypti mosquitoes are the main vector of viruses Dengue, Zika and Chikungunya. Shortly after the first report of the dengue vector Ae. aegypti in Easter Island (Rapa Nui) in late 2000, the first disease outbreak dengue occurred. Viral serotyping during the 2002 outbreak revealed a close relationship with Pacific DENV-1 genotype IV viruses, supporting the idea that the virus most likely originated in Tahiti. Mitochondrial NADH dehydrogenase subunit 4 (ND4) DNA sequences generated from 68 specimens of Ae. aegypti from Easter Island reporting a unique finding of a single maternal lineage of Ae. aegypti on Easter Island.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Dengue; Haplotypes; Rapa Nui.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262016000200186
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Occurrence of 15 haplotypes of linepithema micans (Hymenoptera: Formicidae) in southern Brazil. Repositório Alice
RAMALHO, M. O.; MARTINS, C.; CAMPOS, T.; NONDILLO, A.; BOTTON, M.; BUENO, O. C..
Abstract The ant genus Linepithema is widely known, thanks to the pest species Linepithema humile (Mayr), which is easily mistaken for Linepithema micans (Forel) due to their morphological similarity. Like L. humile , L. micans is associated to the main grapevine pest in Brazil, Eurhizococcus brasiliensis (Wille), also known as ground pearl. Therefore, the present study uses mtDNA fragments to expand the knowledge of haplotype diversity and distri- bution of L. micans in the state of Rio Grande do Sul Brazil), to understand the genetic differences of the popu- lations identified in this study. We identified 15 haplotypes of L. micans spread across different localities. Twelve of these haplotypes were new for the species. The high haplotype diversity...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Mitotype; Mitótipo; Ground pearl; Rio Grande do Sul; Haplotypes; Linepithema micans.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1071592
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PCR-RFLP analysis of non-coding regions of cpDNA in Araucaria angustifolia (Bert.) O. Kuntze Genet. Mol. Biol.
Schlögl,Paulo Sérgio; Souza,Anete Pereira de; Nodari,Rubens Onofre.
The Araucaria angustifolia (Bert.) O. Kuntze, also named the "paraná pine" (‘pinheiro-do-Paranᒠin Portuguese), is a native conifer species naturally occurring in the Brazilian Tropical Atlantic Forest which in Brazil is mostly limited to the southern Brazilian states of Paraná, Santa Catarina and Rio Grande do Sul. Chloroplast DNA markers (cpDNA) are useful in populational genetic studies because of their low substitution rate and the uniparental transmission. The conservation of cpDNA genes between species has allowed the design of consensus chloroplast primers that have had a great impact on population genetics and phylogenetic studies. In this study we used the polymerase chain reaction technique combined with restriction enzyme fragment length...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Haplotypes; Conifer species; Brazilian pine; Genetic diversity.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300020
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Phylogenetic analysis, based on EPIYA repeats in the cagA gene of Indian Helicobacter pylori, and the implications of sequence variation in tyrosine phosphorylation motifs on determining the clinical outcome Genet. Mol. Biol.
Tiwari,Santosh K.; Sharma,Vishwas; Sharma,Varun Kumar; Gopi,Manoj; Saikant,R; Nandan,Amrita; Bardia,Avinash; Gunisetty,Sivaram; Katikala,Prasanth; Habeeb,Md. Aejaz; Khan,Aleem A.; Habibullah,C.M..
The population of India harbors one of the world's most highly diverse gene pools, owing to the influx of successive waves of immigrants over regular periods in time. Several phylogenetic studies involving mitochondrial DNA and Y chromosomal variation have demonstrated Europeans to have been the first settlers in India. Nevertheless, certain controversy exists, due to the support given to the thesis that colonization was by the Austro-Asiatic group, prior to the Europeans. Thus, the aim was to investigate pre-historic colonization of India by anatomically modern humans, using conserved stretches of five amino acid (EPIYA) sequences in the cagA gene of Helicobacter pylori. Simultaneously, the existence of a pathogenic relationship of tyrosine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Helicobacter pylori; EPIYA motifs; Tyrosine phosphorylation motifs; Haplotypes; Anatomically modern humans.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200019
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