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Registros recuperados: 14
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A herpes-like virus infects a non-ostreid bivalve species: virus replication in Ruditapes philippinarum larvae ArchiMer
Renault, Tristan; Lipart, Cecile; Arzul, Isabelle.
Sporadic high mortalities were reported in June 1997 among hatchery-reared larval Manila clam Ruditapes philippinarum in a French commercial hatchery. Cellular abnormalities were observed in semi-thin sections in affected animals. Transmission electron microscopy revealed the presence of herpes-like virus particles in larvae. This is the first description of a herpes-like virus infection in larval R. philippinarum, a non-ostreid bivalve species. Virus particles were similar to other herpes-like viruses described from different oyster species with respect to ultrastructure and morphogenesis. Electron microscopic examination also demonstrated cells with condensed chromatin and extensive perinuclear fragmentation of chromatin. Like viruses infecting oysters,...
Tipo: Text Palavras-chave: Apoptosis; Virus replication; Mortality; Ruditapes philippinarum; Manila clam; Herpes like virus.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-816.pdf
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Apoptose et infections à virus de type herpès chez les bivalves marins : détection de séquences codant pour des protéines inhibitrices de l'apoptose ArchiMer
Didier, Yohann.
Tipo: Text Palavras-chave: PCR; Apoptosis; Marine bivalve; Herpes like virus.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/rapport-1768.pdf
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Concomitant herpes-like virus infections in hatchery-reared larvae and nursery-cultured spat Crassostrea gigas and Ostrea edulis ArchiMer
Renault, Tristan; Le Deuff, Rose-marie; Chollet, Bruno; Cochennec, Nathalie; Gerard, Andre.
Concomitant sporadic high mortalities were reported in France in May 1994 among batches of hatchery-reared larval Pacific oysters Crassostrea gigas and European flat oysters Ostrea edulis in 2 hatcheries, and in June and July 1994 among batches of cultured spat of both species in a shellfish nursery. Histological observation showed the presence of cellular abnormalities in moribund animals. Transmission electron microscopy revealed the presence of herpes-like virus particles in infected larvae and spat of both oyster species. This is the first description of a herpes-like virus infection in larval O.. edulis. Viruses observed in diseased larvae and spat of both species are similar with respect to ultrastructure and morphogenesis, They were detected...
Tipo: Text Palavras-chave: Apoptosis; Virus replication; Concomitant infection; Oyster mortality; Crassostrea gigas; Pacific oyster; Ostrea edulis; European flat oyster; Herpes like virus.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-820.pdf
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Diagnosis of herpes-like virus infections in oysters using molecular techniques ArchiMer
Renault, Tristan; Lipart, Cecile.
Several herpes-like virus infections have been reported since 1991 among different bivalve species around the world (Nicolas et al., 1992; Comps and Cochennec, 1993; Renault et al., 1994; Hine and Thome, 1997; Hine 1997). To date, detection of these infections has been strongly correlated with severe losses of oyster larvae and spat. The virus infections are currently diagnosed by laborious histologicai and transmission electron microscopical examinations. These techniques, however, facilitate neither rapid diagnosis nor the ability to analyse large sample numbers.
Tipo: Text Palavras-chave: Diagnosis; Ostrea edulis; Crassostrea gigas; Herpes like virus.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/acte-2902.pdf
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Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" - Final Report 4th January 1999 to 3rd January 2002 ArchiMer
Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
Little information is available on viral infections that affect bivalve molluscs. Such a lack of data is due ta a certain inadequacy of the diagnosis methods that are employed when massive mortality events occur. Most laboratories involved in mollusc pathology still analyse samples through light microscopy. World-wide, there is thus currently a lack of information concerning the occurrence of bivalve herpesvituses. This is probably due to the lack of suitable diagnostic tools. The basic method for identification and examination of suspect samples is predominantly histopathology. This enables the identification of any cellular changes, but is not conclusive identification of bivalve herpesviruses. This technique doesn't allow, by itself, to detect viruses...
Tipo: Text Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Validation molecular; Detection; Herpesviridae; OsHV-1; Diagnostic techniques; Oysters.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00044/15525/12912.pdf
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Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" - First Periodic Progress Report 4th January 1999 to 3rd January 2000 ArchiMer
Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in bivalves and to validate these reagents by using them in different European laboratories involved in shellfish epidemiological surveys. This will done using techniques developed by the partners to characterise viruses by studying their genomes and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are: 1 - Obtaining the complete oyster herpes-like virus DNA sequence with determination of the genome structure. 2 - Comparing oyster herpes-like viruses with other viruses belonging to the Helpesviridae family on the basis of sequence data and...
Tipo: Text Palavras-chave: Pathology; Diagnosis; Oyster; Herpes like virus; Herpesviridae; Immunology; Detection; Virus; Bivalves.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00044/15523/12910.pdf
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Diagnosis of oyster herpes-like virus: development and validation of molecular immunological and cellular tools "VINO". Second periodic progress report: 4th January 2000 at 3rd January 2001 ArchiMer
Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in bivalves and to validate these reagents by using them in different European laboratories involved in shellfish epidemiological surveys. This will done using teclmiques developed by the patiners to characterise viruses by studying their genome and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are: 1 - Obtaining the complete oyster herpesvirus (OsHV-1) DNA sequence with determination of the genome structure. 2 - Comparing OsHV -1 with viruses belonging to the Herpesviridae family on the basis of sequence data and genome structure. 3 -...
Tipo: Text Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Validation molecular; Detection; Herpesviridae; OsHV-1; Diagnostic techniques; Oysters.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00048/15947/13377.pdf
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Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" Third Periodic Progress Report : 4th January 2001 to 3rd January 2002 ArchiMer
Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
Herpesvirus infections have been reported in different bivalves species around the world. Specific diagnostic tools are needed in order ta assess the causative raIe of these agents in mortalities observed among bivalves There is currently a lack of information concerning the occurrence of bivalve herpesviruses. This is probably due ta the lack of suitable diagnostic tools. The basic method for examination of bivalve samples is predominantly histology. This enables the identification of any cellular changes, but is not conclusive for virus identification. Transmission electron microscopy is a necessity for visual confirmation of viral infections. However, histology and electron microscopy are time consuming and inadequate for epidemiological studies. The...
Tipo: Text Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Virus; Oyster; Detection; OsHV-1; Herpesviridae.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00044/15524/12911.pdf
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Etude des mécanismes antiviraux développés par les huîtres lors d'infections à virus de type herpès ArchiMer
Olicard, Cecile.
Tipo: Text Palavras-chave: Cytometry; Herpes like virus; Oyster; Antivirus mechanisms.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/rapport-1769.pdf
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Experimental herpes-like viral infections in marine bivalves: demonstration of interspecies transmission ArchiMer
Arzul, Isabelle; Renault, Tristan; Lipart, Cecile.
Since 1972, herpes-like virus infections have been reported in several marine bivalve species around the world. Viral detection was often associated with high mortality rates in larvae and spat. To determine whether a single virus is able to infect different bivalve host species, we carried out experimental transmission assays. As a first step, 8 assays were performed to infect axenic Crassostrea gigas larvae with virus from infected C. gigas larvae using a previously described protocol. The protocol appeared reliable and PCR was confirmed as a powerful technique for detecting viral DNA in experimentally infected oysters. The defined protocol was then applied to infect different bivalve species. Interspecies viral transmission was demonstrated under...
Tipo: Text Palavras-chave: Experimental infection; Host specificty; Interspecies transmission; Marine bivalves; Herpes like virus.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-819.pdf
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Experimental transmission of a Herpes-like virus to axenic larvae of Pacific oyster, Crassostrea gigas ArchiMer
Le Deuff, Rose-marie; Nicolas, Jean-louis; Renault, Tristan; Cochennec, Nathalie.
A herpes-like virus which was associated with mortalities amongst hatchery-reared larvae of Pacific oysters, Crassostrea gigas. was observed for the first time in France during the summer 1991 (Nicolas et al.. 1992). Since this first report, we have noted further outbreaks of the disease in the summers of 1992 and 1993 in hatcheries on the western coasts of France in which a similar virus was associated with moribund larvae. Moreover, in the summer 1991, Hine et al. (1992) described a herpes-like virus responsible for larval mortality of hatchery reared C. gigas, in New Zealand. Ail these viruses are c10sely related with respect to their size and morphology and to their apparent cellular tropism for fibroblastic cells.
Tipo: Text Palavras-chave: Transmission; Herpes like virus; Crassostrea gigas; Oysters.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/publication-2887.pdf
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Herpes-like virus infecting Japanese oyster (Crassostrea gigas) spat ArchiMer
Renault, Tristan; Cochennec, Nathalie; Le Deuff, Rose-marie; Chollet, Bruno.
During the summer of 1993, abnormal sporadic mass mortalities (80-90%) and morbidities occurred among four batches of young Japanese oyster (Crassostrea gigas) spat from the French Atlantic coast. These mortalities were only observed in July. Samples of affected animaIs were examined by electron microscopy. This paper reports the presence and appearance of herpes viruses in these samples.
Tipo: Text Palavras-chave: Herpes like virus; Mortalities; Crassostrea gigas; Oysters.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/publication-2888.pdf
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Herpesviruses associated with mortalities among Pacific oyster, Crassostrea gigas, in France-Comparative study ArchiMer
Renault, Tristan; Le Deuff, Rose-marie; Cochennec, Nathalie; Maffart, Patricia.
Sporadic mortalities were reported in june and july of 1992 and 1993 among batches of hatchery-reared larval Pacifie oyster, Crassastrea gigas, and at the beginning of July and in August of 1993 among five batches of 3-7 month old young spats, C. gigas. Observations with transmission electron microscope showed the presence of herpes-like virus particles in infected larvae and young spats. The viruses observed in diseased larvae and affected young spat oysters were very similar. Elevated temperature and crowding may increase susceptibility of oysters to these herpes-like virus infections.
Tipo: Text Palavras-chave: Spat; Larvae; Crassostrea gigas; Pacific oysters; Mortality; Herpes like virus; Naissain; Larve; Crassostrea gigas; Huître japonaise; Mortalités; Herpès like virus.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/publication-2898.pdf
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Report about mollusc diseaes ArchiMer
Berthe, Franck.
Of the 75 laboratories contacted through the survey, 54 answered, 14 of which are partially or totally devoted to mollusc diseases. In fact, only 4 laboratories are devoted full-time to this work. Of the 14 laboratories that declared to work on the diagnosis of mollusc diseases, 12 stated that they worked with mussels, 12 with oysters, 9 with clams and 8 with other mollusc species. This is coherent with available production information. According to production data in 2001 (FAO source), the main mollusc species in the region are the blue mussel, Mytilus edulis (297,485 tonnes), the Mediterranean mussel, Mytilus galloprovincialis (131,014 tonnes), the Pacific cupped oyster, Crassostrea gigas (127,323 tonnes, an important part of which is produced on the...
Tipo: Text Palavras-chave: Bonamia existiosus; Bonamis ostreae; Bonamiosis; Marteilia sydneyi; Marteilia maurini; Marteilia refringens; Marteiliosis; Perkinsus marinus; Perkinsus olseni; Perkinsus atlanticus; Perkinsosis; Vibriosis; Herpes like virus; Oysters; Pathology; Mollusc diseases.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-3300.pdf
Registros recuperados: 14
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