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1,3-Propanediol production from crude glycerol by Clostridium butyricum DSP1 in repeated batch Electron. J. Biotechnol.
Szymanowska-Powalowska,Daria.
Background The production of biofuels from renewable energy sources is one of the most important issues in industrial biotechnology today. The process is known to generate various by-products, for example crude glycerol, which is obtained in the making of biodiesel from rapeseed oil. Crude glycerol may be utilized in many ways, including microbial conversion to 1,3-propanediol (1,3-PD), a raw material for the synthesis of polyesters and polyurethanes. Results The paper presents results of a study on the synthesis of 1,3-propanediol from crude glycerol by a repeated batch method with the use of Clostridium butyricum DSP1. Three cycles of fermentation medium replacement were carried out. The final concentration of 1,3-PD was 62 g/L and the maximum...
Tipo: Journal article Palavras-chave: Environmental stress; Fermentation; High-performance liquid chromatography.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600011
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Alkaloids and phenolics biosynthesis increases mango resistance to infection by Ceratocystis fimbriata Bragantia
Araujo,Leonardo; Bispo,Wilka Messner Silva; Rios,Jonas Alberto; Fernandes,Sergio Antonio; Rodrigues,Fabrício de Ávila.
ABSTRACT Mango wilt, caused by Ceratocystis fimbriata, is one of the most important diseases affecting mango yields in Brazil. Information regarding the biochemical mechanisms involved in mango resistance against C. fimbriata is absent in the literature. Thus, the present study determined and quantified alkaloids and phenolics in the stem tissue of mango plants from Palmer (susceptible) and Ubá (resistant) cultivars. Furthermore, it was examined the effect of these secondary metabolites against C. fimbriata growth in vitro. The high-performance liquid chromatography revealed that the concentration of two alkaloids (theobromine and 7-methylxanthine) and six phenolic compounds (caffeic acid, p-coumaric acid, gallic acid, protocatechuic acid, catechin and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ceratocystis fimbriata; Mangifera indica; High-performance liquid chromatography; Host defense responses; Secondary metabolism in plants; Vascular pathogen.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052016000200199
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Assessment of the antiproliferative and antigenotoxic activity and phytochemical screening of aqueous extracts of Sambucus australis Cham. & Schltdl. (ADOXACEAE) Anais da ABC (AABC)
TEDESCO,MARÍLIA; KUHN,ANDRIELLE W.; FRESCURA,VIVIANE DAL-SOUTO; BOLIGON,ALINE A.; ATHAYDE,MARGARETH L.; TEDESCO,SOLANGE B.; SILVA,ANTONIO C.F. DA.
ABSTRACT The purpose of this study was to evaluate the antiproliferative and antigenotoxic activity of Sambucus australis Cham. & Schltdl. aqueous extracts on the cell cycle of Allium cepa L. as well as determine the phenolic compounds in such extracts. S. australis inflorescences and leaves of two accessions were used for aqueous extract preparation at concentrations: 0.003 g/ml and 0.012 g/ml. A. cepa bulbs were rooted in distilled water and, subsequently, placed in treatments for 24 hours. Rootlets were collected and fixed in modified Carnoy’s solution for 24 hours and kept. The squash technique was performed for slide preparation. Root tips were smashed and stained with 2% acetic orcein, and a total of 4000 cells per treatment were analyzed. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plants; Mitotic index; Allium cepa; Sabugueiro; High-performance liquid chromatography.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502141
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Occurrence of aflatoxin B1 in natural products BJM
Prado,Guilherme; Altoé,Aline F.; Gomes,Tatiana C. B.; Leal,Alexandre S.; Morais,Vanessa A. D.; Oliveira,Marize S.; Ferreira,Marli B.; Gomes,Mateus B.; Paschoal,Fabiano N.; Souza,Rafael von S.; Silva,Daniela A.; Madeira,Jovita E. G. Cruz.
The media claims for the consumption of natural resource-based food have gradually increased in both developing and developed countries. The interest in the safety of these products is partially due to the possible presence of toxigenic fungi acting as mycotoxin producers, such as aflatoxins produced during the secondary metabolism of Aspergillus flavus, A. parasiticus and A. nomius. Aflatoxins, mainly aflatoxin B1, are directly associated with liver cancer in human beings. This paper is aimed at evaluating the presence of aflatoxin B1 in a few vegetable drugs, dried plant extracts and industrialized products traded in 2010 in the city of Belo Horizonte, State of Minas Gerais, Brazil. The method used for the quantification of aflatoxin B1 was based on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plants; Fungi; Mycotoxin; High-performance liquid chromatography; HPLC.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400026
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Stability-indicating HPLC method for determination of amiodarone hydrochloride and its impurities in tablets: a detailed forced degradation study BJPS
Coelho,Ana Silva; Ribeiro,Igor Felipe Rodrigues; Lages,Eduardo Burgarelli.
Resumo Amiodarone hydrochloride is one of the most important drugs used to treat arrhythmias. The USP monograph for amiodarone hydrochloride describes an HPLC method for the quantification of seven impurities, however, this method shows problems that result in unresolved peaks. Moreover, there is no monograph for tablets in this compendium. Thus, a stability indicating HPLC method was developed for the determination of amiodarone, its known impurities and degradation products in tablets. A detailed forced degradation study was performed submitting amiodarone API, tablets and placebo to different stress conditions: acid and alkaline hydrolysis, oxidation, metal ions, heat, humidity, and light. Amiodarone hydrochloride API was susceptible to degradation in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amiodarone hydrochloride; Impurities; Forced degradation; Stability indicating methods; High-performance liquid chromatography.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100552
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