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A reduced, yet functional, nectary disk integrates a complex system of floral nectar secretion in the genus Zeyheria (Bignoniaceae) Acta Botanica
Machado,Silvia Rodrigues; Souza,Camila Vaz de; Guimarães,and Elza.
ABSTRACT The genus Zeyheria (Bignoniaceae) comprises only two species, both of which have been described as possessing a reduced and non-functional nectary disk. Despite the importance of this evolutionary change in the floral nectary, these functional assumptions have been based on disk size and on the distribution, abundance and histochemistry of corolla-borne trichomes. By combining methods on light and electron microscopy, here we investigated the functionality of the reduced nectary disk and describe all of the tissues and structures of the nectar chamber in order to determine the sites of floral nectar secretion in both Zeyheria species. . Our data find the floral nectary traits of both species to be very similar, although differing in their cellular...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bignoniaceae; Floral nectary; Functional anatomy; Histochemistry; Ultrastructure.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000300344
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Age Related Structural Changes in the Bulbourethral Gland of Gaddi Goat (Capra hircus) International Journal of Morphology
Archana,P; Katiyar,R. S; Sharma,D. N; Farooqui,M. M; Prakash,Ajay.
A study was conducted on the bulbourethral glands of 30 Gaddi goats, divided into three groups of 10 animals in each viz; Prepubertal (1 day old to < 18 months of age), Pubertal (18 months to < 5yrs of age) and Postpubertal (>5yrs of age). The study revealed that the glands grew at a faster speed up-to 6 months of postnatal life after which the pace slowed down considerably. Almost 10 times increase in weight and more than three times increase in all the linear mensurations were observed in prepubertal group from the time of birth. The stroma vs parenchyma ratio which was 80:20 in one-day-old kids showed three times increase in parenchyma in prepubertal animals, and the ratio became 43:57. In one day old kids luminated and non-luminated secretory...
Tipo: Journal article Palavras-chave: Bulbourethral gland; Gaddi goat; Histology; Histochemistry.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200049
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All green, but equal? Morphological traits and ecological implications on spores of three species of mosses in the Brazilian Atlantic forest Anais da ABC (AABC)
MACIEL-SILVA,ADAÍSES S.; SILVA,FLÁVIA C.L. DA; VÁLIO,IVANY F.M..
Spores of the tropical mosses Pyrrhobryum spiniforme, Neckeropsis undulata and N. disticha were characterized regarding size, number per capsule and viability. Chemical substances were analyzed for P. spiniforme and N. undulata spores. Length of sporophyte seta (spore dispersal ability) was analyzed for P. spiniforme. Four to six colonies per species in each site (lowland and highland areas of an Atlantic Forest; Serra do Mar State Park, Brazil) were visited for the collection of capsules (2008 – 2009). Neckeropsis undulata in the highland area produced the largest spores (ca. 19 µm) with the highest viability. The smallest spores were found in N. disticha in the lowland (ca. 13 µm). Pyrrhobryum spiniforme produced more spores per capsule in the highland...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bryophytes; Histochemistry; Life histories; Spore size; Spore production; Tropical rain forest.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301249
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Anatomical, phytochemical and histochemical study of Solidago chilensis Meyen Anais da ABC (AABC)
SOUZA,DOUGLAS M.F. DE; SÁ,RAFAELA D.; ARAÚJO,EVANI L.; RANDAU,KARINA P..
ABSTRACT Solidago chilensis Meyen, belonging to the family Asteraceae, is a plant native to South America and the only representative of the genus in Brazil. This species is popularly known as “arnica” and is used to treat bruises, muscle pain and inflammation. The objective of this work was to contribute to the pharmacognostic standardization of S. chilensis. Cross-sections were obtained, by freehand, for microscopic analysis of root, stem and leaf; for these parts of the plant maceration was also performed according to the method of Jeffrey. For the leaf were still made paradermal sections, scanning electron microscopy analysis, phytochemical and histochemical tests. Thus, it was determined anatomical features useful for diagnosis of the species which,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anatomy; Arnica; Asteraceae; Histochemistry; Phytochemistry; Solidago chilensis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502107
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Anatomy, ultrastructure and secretion of Hibiscus pernambucensis Arruda (Malvaceae) extrafloral nectary Rev. Bras. Bot.
Rocha,Joecildo Francisco; Machado,Silvia Rodrigues.
This paper reports on the extrafloral nectary (EFN) of Hibiscus pernambucensis, a native shrub species occurring in mangrove and restinga along Brazil's coastline. EFNs occur as furrows with a protuberant border on the abaxial surface veins of the leaf blade. Each nectary consists of numerous secretory multicellular trichomes, epidermal cells in palisade-like arrangements and non-vascularized parenchyma tissue. Nectar secretion is prolonged, since secretion starts in very young leaves and remains up to completely expanded leaves. Reduced sugars, lipids, and proteins were histochemically detected in all the nectary cells; phenolic substances were detected in the vacuoles of the epidermal palisade cells and in some secretory trichome cells. The secretory...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anatomy; Extrafloral nectary; Hibiscus pernambucensis; Histochemistry; Ultrastructure.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042009000300008
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Androgen Receptor Distribution, PAS and Alcyan Blue Reaction in the Vomeronasal Organ and the Nasal Septum Mucosa of the Developing Male Rat International Journal of Morphology
Núñez Chichet,M E; Genovese,P; Bielli,A.
The vomeronasal organ (VNO) is a tubular chemoreceptory organ which detects environmental pheromones and is essential for mammalian normal reproductive activity. A sensitive chemoreceptory neuroepithelium lines the concave VNO wall and the opposite, convex wall is lined by a seudostratified, receptor-free epithelium. The secretion of the Jacobson glands (JG) -tubuloacinary glands lying in the lamina propia of the VNO - is essential for pheromones contacting vomeronasal organ chemoreceptors. Tubuloacinary glands lying in the connective tissue of the nasal septum mucosa (Bowman glands), can be classified as ventral and dorsal, according to their location. Positivity to PAS and Alcyan blue reactions and androgen receptor immunolocalization was evaluated with...
Tipo: Journal article Palavras-chave: Vomeronasal organ; Chemoreception; Development; Histochemistry; Immunohistochemistry.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300018
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Antiherbivory defense mechanisms along an environmental gradient in restinga Acta Botanica
Silva,Maiara Matilde da; Boeger,Maria Regina Torres; Melo Júnior,João Carlos Ferreira de; Santos,Bruno Francisco Sant’Anna dos.
ABSTRACT Plant-herbivore interactions have directed the evolution of defense mechanisms in plants, which can vary intraspecifically according to environmental quality. This study evaluated variation in the production of antiherbivory defenses in plant species distributed along an environmental gradient of restinga. The study was performed in Parque Estadual Acaraí, state of Santa Catarina, Brazil. Three species of four plant formations with different abiotic conditions were selected for study. Specific leaf area; water, nitrogen and carbon content; and presence of phenols, tannins, lignin and alkaloids, were evaluated in leaves of ten plants per species per plant formation. The results indicated that Symphyopappus casarettoi possesses Low Nutritional...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coastal plain; Herbivore-plant interaction; Histochemistry; Parque Estadual Acaraí; Physical defense; Secondary metabolites.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000400583
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Aspectos histológicos e histoquímicos da cloaca feminina de Columba livia (Gmelin) (Columbidae, Columbiformes) Zoologia
Teles,Maria Eloíza de Oliveira; Ribeiro,Maria das Graças; Maruch,Sandra Maria das Graças; Ribeiro,Roseli Deolinda.
The domestic dove Columba livia (Gmelin, 1789) is a species well adapted to Brazil and the study of its reproductive biology is part of a broad research project on birds. This essay describes the morphological aspects of the cloaca of female Columba livia, describing compartments lengthwise starting from the head such as the coprodeo, urodeo and proctodeo limited by mucosal folds. Each compartment of the cloaca presents its own morphological characteristics which differ one from the other in form, height and position of mucosal projections, kinds of tissues, presence and histological aspects of glands, presence of lymphonodus. The rectum opens into the coprodeo, the ureter opens into the oviduct and the cloacal sac opening into the proctodeo. Histochemical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Domestic dove; Morphological aspects; Histochemistry; Cloaca.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000100013
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Aspectos reprodutivos de Sphoeroides greeleyi (Gilbert), Pisces, Osteichthyes, Tetraodontidae, da gamboa do Baguaçu, Baia De Paranaguá, Paraná, Brasil Zoologia
Schultz,Yart Damasceno; Favaro,Luís Fernando; Spach,Henry Louis.
The present study analyses the reproductive aspects of Sphoeroides greeleyi (Gilbert, 1900), such as morphology and histochemistry of carbohydrates in the oogenesis phases, maturity scale, time and type of spawn, sexual rate and first ripened mature size for females. From the monthly captured animais (Nov 1998 to Nov 1999) the morphometric data (entire length and total weight) were extracted and the sex and the gonadal maturity were determined. Some ovaries were prepared for microscopic analysis that charac-terized six phases for the development of the ovarian folheies. Through the carbohydrates analysis the acid carbohydrates could be related to the oogenesis maintenance and the neutral carbohydrates to the beginning of embryonic development. A maturity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Reproduetion; Histology; Histochemistry.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752002000100004
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Clinical and Histochemical Alterations of the Periodontal Ligament in Gerbils after Malocclusion Induced International Journal of Morphology
Naves,Leandro Moura Leite; Issa,João Paulo Mardegan; Pitol,Dimitrius Leonardo; Fukada,Sandra Yasuyo; di Matteo,Miguel Ángel Sala; Iyomasa,Mamie Mizusaki.
The aim of this article is to show the clinical and histochemical alterations of the first periodontal ligament, on the right side, after upper molars teeth extraction on the left side in gerbils. After two months, the periodontal ligaments were removed and processed for histochemical analysis. The data showed that TRAP reaction was able to evidence the osteoclastic activity in the hyperfunction hemimandible, right side, explaining the functional changes in the periodontal ligament after teeth extraction, and a little gingival recession and radicular exposure of teeth without function was observed at inferior molars of the left side
Tipo: Journal article Palavras-chave: Periodontal ligament; Histochemistry; Malocclusion; Gerbil; Meriones unguiculatus.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000400036
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Comparative analysis of the integument of different tree frog species from Ololygon and Scinax genera (Anura: Hylidae) Rev. Bras. Zool.
Silva,Henrique Alencar Meira da; Silva-Soares,Thiago; Brito-Gitirana,Lycia de.
ABSTRACT The integuments of ten treefrog species of two genera from Scinaxnae - O. angrensis (Lutz, 1973), O. flavoguttata (Lutz & Lutz, 1939), O. humilis (Lutz & Lutz, 1954), O. perpusilla (Lutz & Lutz, 1939), O. v-signata (Lutz, 1968), Scinax hayii (Barbour, 1909), S. similis (Cochran, 1952), O. trapicheroi (Lutz & Lutz, 1954) and S. x-signatus (Spix, 1824) - were investigated using conventional and histochemical techniques of light microscopy, and polarized light microscopy. All integuments showed the basic structure of the anuran integument. Moreover, the secretory portions of exocrine glands, such as serous merocrine and apocrine glands, were found to be restricted to the spongious dermis. Lipid content occurred together with the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazilian Atlantic forest; Histochemistry; Hylids; Treefrog.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702017000100327
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Comparative anatomy of calyx and foliar glands of Banisteriopsis C. B. Rob. (Malpighiaceae) Acta Botanica
Araújo,Josiane Silva; Meira,Renata Maria Strozi Alves.
Banisteriopsis is considered one of the largest genera of Malpighiaceae with 58 species, of which 47 occur in Brazil. The typical calyx and leaf glands of Banisteriopsis are considered relevant to the adaptive success of Malpighiaceae. Comparative studies of anatomical and histochemical characteristics may reveal similarities and assist in the interpretation of the functions performed by such glands. The present study aimed to describe the anatomy of the calyx and leaf glands of 38 species ofBanisteriopsis that occur in Brazil, and to analyze these structures histochemically in B. campestris, B. laevifolia and B malifolia, using standard methods. Calyx glands differ from leaf glands by possessing an irregular surface that is covered by a thick cuticle that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Banisteriopsis; Calyx glands; Histochemistry; Leaf glands; Lipids; Micromorphology; Polysaccharides; Protein.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016000100112
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Comparative Intestinal Histomorphology of Five Species of Phyllostomid Bats (Phyllostomidae, Microchiroptera): Ecomorphological Relations with Alimentary Habits International Journal of Morphology
Gadelha-Alves,Rafaela; Rozensztranch,Andréa Melo Da Silva; Rocha- Barbosa,Oscar.
The intestines of Sturnira lilium, Phyllostomus hastatus, Carolliaperspicillata, GlossophagasoricinaíindDesmodus rotundus were analized through macroscopic, morphometric and light microscopy studies. The species studied presented some common charactenstics generally found in bats, such as the absence of a caecum and an appendix. The frugivorous bat S. lilium has a longer intestine than the other species. The intestinal epithelium is of the simple columnar type, constituted of absorptive cells characterized by microvilli and goblet cells, which in all the species proved to be PAS + and AB +. These cells presented some differences in quantity and in distribution among the species. The intestinal mucosa presented morphologic and morphometric differences, such...
Tipo: Journal article Palavras-chave: Intestines; Bats; Phyllostomidae; Histology; Histochemistry; Micrometry; Ecomorphology.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000300014
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Corona development and floral nectaries of Asclepiadeae (Asclepiadoideae, Apocynaceae) Acta Botanica
Monteiro,Mariana Maciel; Demarco,Diego.
ABSTRACT Flowers of Asclepiadoideae are notable for possessing numerous nectaries and elaborate coronas, where nectar can accumulate but is not necessarily produced. Given the complexity and importance of these structures for reproduction, this study aimed to analyze the ontogeny of the corona, the structure and position of nectaries and the histochemistry of the nectar of species of Asclepiadeae. Two types of coronas were observed: androecial [C(is)] and corolline (Ca). The development of the C(is)-type of corona initiates opposite the stamens in all species examined with the exception of Matelea in which it begins to develop as a ring around the filament tube. Despite their morphological variation, coronas typically originate from the androecium. A...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Asclepiads; Histochemistry; Ontogeny; Nectar; Structural; Diversity.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000300420
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Development and some histochemical aspects of foliar glandular trichomes of Stevia rebaudiana (Bert.) Bert. - Asteraceae Rev. Bras. Bot.
MONTEIRO,WALKYRIA ROSSI; CASTRO,MARÍLIA DE MORAES; MAZZONI-VIVEIROS,SOLANGE CRISTINA; MAHLBERG,PAUL GORDON.
The ten-celled biseriate glandular trichome of Stevia rebaudiana (Bert.) Bert.-Asteraceae, found on both leaf surfaces, originates from a single protruding, protodermal cell undergoing an anticlinal division. A subsequent series of periclinal divisions, occurring in acropetal sequence, leads to the formation of the trichome, composed of five pairs of cells, one pair of basal cells, another of stalk cells and three pairs of secretory head cells. Developing, still two-celled glandular trichomes already occur on leaf primordia of the second pair (these primordia measuring, in some cases, ca. 0.30 mm in length), and most of the glandular trichomes are at the mature phase on very young, expanding leaves, for example on those of the sixth pair. The secretory...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stevia rebaudiana; Asteraceae; Glandular trichomes; Ontogeny; Histochemistry.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000300013
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Dynamique et enzymologie de la digestion chez l'huitre Crassostrea gigas ArchiMer
Boucaud, C; Lebesnerais, C; Lubet, P; Lihrmann, I.
Feeding experiments carried out on Crassostrea gigas allowed the authors to set up the stages and the timing of the movement of food throughout the digestive tract. Various activities involved in digestion (glycosidases, esterases, and peptidases) were investigated histochemically in the digestive tract in order to localize the sites of absorption and the sites of protein and carbohydrate digestion. An explicative model of the different stages of digestion is proposed.
Tipo: Text Palavras-chave: Crassostrea gigas; Bivalvia; Cytology; Histochemistry; Histology; Digestive system; Enzymatic activity; Digestive glands; Digestion; Enzymes; Transit digestif; Digestion; Huitre; Crassostrea gigas.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1179.pdf
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Effect of salbutamol on innervated and denervated rat soleus muscle BJMBR
Šoic-Vranic,T.; Bobinac,D.; Bajek,S.; Jerkovic,R.; Malnar-Dragojevic,D.; Nikolic,M..
The objective of the present investigation was to perform a 14-day time-course study of treatment with salbutamol, a ß2 adrenoceptor agonist, on rat soleus muscle in order to assess fiber type selectivity in the hypertrophic response and fiber type composition. Male Wistar rats were divided into four groups: control (N = 10), treated with salbutamol (N = 30), denervated (N = 30), and treated with salbutamol after denervation (N = 30). Salbutamol was injected intraperitoneally in the rats of the 2nd and 4th groups at a concentration of 0.3 mg/kg twice a day for 2 weeks. The muscles were denervated using the crush method with pean. The animals were sacrificed 3, 6, 9, 12, and 14 days after treatment. Frozen cross-sections of soleus muscle were stained for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salbutamol; Rat skeletal muscle; Soleus muscle; Denervation; Histochemistry; Muscle fibers.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200008
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Effects of sciatic nerve transection on ultrastructure, NADPH-diaphorase reaction and serotonin-, tyrosine hydroxylase-, c-Fos-, glucose transporter 1- and 3-like immunoreactivities in frog dorsal root ganglion BJMBR
Rigon,F.; Rossato,D.; Auler,V.B.; Dal Bosco,L.; Faccioni-Heuser,M.C.; Partata,W.A..
Frogs have been used as an alternative model to study pain mechanisms. Since we did not find any reports on the effects of sciatic nerve transection (SNT) on the ultrastructure and pattern of metabolic substances in frog dorsal root ganglion (DRG) cells, in the present study, 18 adult male frogs (Rana catesbeiana) were divided into three experimental groups: naive (frogs not subjected to surgical manipulation), sham (frogs in which all surgical procedures to expose the sciatic nerve were used except transection of the nerve), and SNT (frogs in which the sciatic nerve was exposed and transected). After 3 days, the bilateral DRG of the sciatic nerve was collected and used for transmission electron microscopy. Immunohistochemistry was used to detect...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Axotomy; Immunoreactivity; Histochemistry.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600513
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Extrafloral nectaries of annatto ( Bixa orellana L.): anatomy, nectar composition and activity during organ development Acta Botanica
Miranda,Rafaela Marques de; Nery,Lays Araújo; Ventrella,Marília Contin.
ABSTRACT This study aimed to anatomically characterize the extrafloral nectaries (EFNs) of annatto (Bixa orellana) and determine the composition of its nectar in order to better understand their structure and function during organ development. Standard light and scanning electron microscopy techniques were used for anatomical analysis, and test-strips and a refractometer for determining nectar composition. Both receptacle and stem EFNs were found to possess similar anatomy and nectar composition, and to secrete from early to advanced developmental stages of the organs to which they are associated. EFNs consisting of uniseriate epidermis, nectariferous parenchyma and subnectariferous parenchyma were found located where vascular tissue is immersed. Some...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ants; Histochemistry; Nectar release; Secretory structure; Urucum.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000300468
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Histochemical aspects of wheat resistance to leaf blast mediated by silicon Scientia Agricola
Silva,Washington Luís da; Cruz,Maria Fernanda Antunes; Fortunato,Alessandro Antônio; Rodrigues,Fabrício Ávila.
Blast, caused by Pyricularia oryzae, has become a significant disease threat to wheat (Triticum aestivum L.) in Brazil. This study aimed to investigate at the histochemical level if silicon (Si) could enhance the production of flavonoids in the leaves of wheat plants in response to P. oryzae infection. Plants from the Aliança cultivar, which are susceptible to blast, were grown in hydroponic cultures containing 0 (-Si) or 2 mM of Si (+Si) and inoculated by spraying a conidial suspension of P. oryzae (1 × 105 conidia mL−1) on all adaxial leaf surfaces of plants at 60 days after emergence (growth stage 65). The fourth and fifth leaves of each plant were used to evaluate blast severity at 24, 36, 48, 72 and 96 h after inoculation (hai). At 96 hai, leaves were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pyricularia oryzae; Flavonoids; Histochemistry; Phenolics.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000400322
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