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A Comparative Study of Fixatives for Axolotl Blastema Tissue International Journal of Morphology
Keskin,Ilknur; Yildirim,Berna; Kolbasi,Bircan; Öztürk,Gürkan; Demircan,Turan.
Regeneration is defined as tissue renewal and functional restoration process of the damaged parts of the body after an injury. Ambystoma mexicanum, commonly named the Axolotl, is one of the unique vertebrates, which has a remarkable ability to regenerate their extremities following the amputation. Although the process of regeneration includes several periods, it can be divided into two main phases; blastema formation and dedifferentiation. In the couple of hours following the amputation, wound closure occurs by migration of epithelial cells around the amputation site followed by macrophage infiltration and dedifferentiation of cells to turn into stem cells. Accumulated stem cells form a very authentic tissue type called blastema, which is crucial for...
Tipo: Journal article Palavras-chave: Axolotl; Blastema; Fixatives; Histology.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100009
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A histological study of microsporogenesis in Tarenna gracilipes (Rubiaceae) Naturalis
Vinckier, S.; Smets, E..
We studied the microsporogenesis in Tarenna gracilipes (Hayata) Ohwi, with special attention to the mode of exine deposition and tapetum development. We based this research on light (LM), scanning electron (SEM) and transmission electron microscopic (TEM) observations of developing anthers of T. gracilipes, from the microspore mother cell stage towards anther dehiscence. Evidence is supplied that the microsporogenesis in T. gracilipes can be considered as simultaneous. Columellae, foot layer and tectum develop in a fibrillar matrix. Similar with earlier studies in Rubiaceae species, a single white line formed near the plasmalemma in the extra-apertural region. The developing endexine dilated into several white line centered lamellae at the apertures. An...
Tipo: Article / Letter to the editor Palavras-chave: Tarenna gracilipes; Rubiaceae; Microsporogenesis; Exine deposition; Tapetum development; Histology; Light microscopy; Scanning.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/407305
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A histological study on the development of the digestive system of Pseudosciaena crocea larvae and juveniles ArchiMer
Mai, Kangsen; Yu, Hairui; Ma, Hongming; Duan, Qingyuan; Gisbert, Enric; Zambonino, Jose-luis; Cahu, Chantal.
The ontogenetic development of the gut and accessory organs in large yellow croaker Pseudosciaena crocea was investigated using light microscopy from hatching up to the juvenile stage (40 days post hatch, dph). At 3 dph (mean +/- S.D., 4.1 +/- 0.1 mm total length, L-T), coinciding with the buccopharynx opening, larvae started to feed exogenously, and the gut consisted of a well-developed buccopharynx, a partially-differentiated oesophagus and an intestine divided in three regions (anterior intestine, intermediate intestine and rectum). Yolk reserves were not completely depleted at the onset of exogenous feeding, and a period of mixed nutrition was observed up to 6 dph (4.3 +/- 0.1 mm L-T) when yolk was definitively exhausted. Important morphological...
Tipo: Text Palavras-chave: Pseudosciaena crocea; Ontogenetic development; Large yellow croaker; Histology; Digestive system.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-930.pdf
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A Macroanatomical and Histological Study of the Uropygial Gland in the White Stork (Ciconia cicionia) International Journal of Morphology
Kozlu,Tolunay; Bozkurt,Yesim Akaydin; Ates,Sevinc.
The present study was aimed at the macroanatomical and histological investigation and the demonstration of the structural characteristics of the uropygial gland in the white stork. The uropygial gland of two adult male white storks constituted the material of the study. It was determined that the gland was situated subcutaneously in between the caudal aspect of the lumbosacral bone and the first coccygeal vertebrae. The gland was composed of two lobes, which displayed an oval appearance when viewed from above and resembled a water drop when observed medially. Tuft of feathers were present on the caudal margin of each lobe. The present study revealed that the uropygial gland of the white stork is a simple tubular gland, which discharges its secretion into...
Tipo: Journal article Palavras-chave: White stork; Uropygial gland; Anatomy; Histology.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300010
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A shortcut method for sexing juvenile European sea bass, Dicentrarchus labrax L. ArchiMer
Menu, Bruno; Peruzzi, Stefano; Vergnet, Alain; Vidal, Marie-odile; Chatain, Beatrice.
The objective of this study was to develop a rapid and effective method of sexing juvenile sea bass with minimum labour and material. To this end, the gonad squash mount technique was applied along with macroscopic techniques for sexing a large number of experimental fish at the age of 215-275 days post fertilization (p.f.). At this age, 90% of the 3894 fish could be unambiguously sexed by macroscopic examination of their gonads, whereas the remaining proportion was identified using squash mount preparations (10%). The accuracy of the observations was measured up to classical histology procedures. Undifferentiated fish accounted for 0.1% of the total population only. The application of the squash mount technique holds out the prospect of considerable...
Tipo: Text Palavras-chave: Histology; Squash; Juvenile; Gonads; Seabass; Dicentrarchus labrax.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-378.pdf
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Academic Achievement and Perception of Two Teaching Methods in Histology: Light Microscopy and Digital Systems International Journal of Morphology
Becerra G,Daniela; Grob L. P,Melisa; Rodríguez R,Ángel; Barker M,María José; Consiglieri L,Lucas; Ferri G,Giorgio; Sabag S,Natividad.
With the advent of digital systems, the role of the microscope as an irreplaceable instrument in the practical teaching of histology has been called into question. In this study academic performance and student perception for three learning methods was compared: digital systems, microscopy, and microscopy plus digital systems, in the muscle tissue unit of the morphology course for first-year dentistry at the Universidad de los Andes, Santiago, Chile. Ninety-five students were divided into 3 groups: Group 1: individual optical microscopy, Group 2: digital systems (one projector per room), and Group 3: microscopy plus digital systems. All participants observed the same striate muscle, cardiac striated muscle, and smooth muscle mounts. Their diagnostic...
Tipo: Journal article Palavras-chave: Medical education; Histology; Visual learning; Virtual slides; Microscopy.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300001
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Acute oral toxicity and anti-inflammatory activity of hydroalcoholic extract from Lampaya medicinalis Phil in rats Biol. Res.
Morales,Glauco; Paredes,Adrián; Olivares,Alberto; Bravo,Jaime.
BACKGROUND: Algesia and inflammation are related with several pathological conditions. It is known that many drugs available for the treatment of these problems cause unwanted side effects. This study was aimed at evaluating acute toxicity and anti-inflammatory activity of Lampaya medicinalis Phil. (Verbenaceae) widely used in the folk medicine of Northern Chile against rheumatism, arthritis and body joints pain. RESULTS: Oral administration of hydroalcoholic extract (HAE) at the highest dose of 3000 mg/ Kg body weight resulted in no mortalities or evidence of significant behavioral changes. Histological examination revealed normal architecture and no significant adverse effects were observed on the liver, kidney, heart, lung or ovaries and testicles. The...
Tipo: Journal article Palavras-chave: Lampaya medicinalis; Hydroalcoholic extract; Acute oral toxicity; Histology; Hematology; Anti-inflammatory; Carrageenan.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100006
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Adaptaciones morfológicas de la lengua de Desmodus Rotundus Rotundus (Chiroptera: Phyllostomidae) en función de la alimentación Mastozool. neotrop.
Mollerach,Marcos I.; Mangione,Susana.
An analysis of the external morphology and histomorphology of the tongue, as well as an histochemical analysis of the glands of Desmodus rotundus, are here presented with the purpose of establishing a correlation between these structures and the feeding habits of this species. In the lingual surface they have the typical filiform papillae modified to different degrees of keratinization, and there are fungiform-like papillae in less amount. The intrinsic musculature and the presence of lynphocitary infiltration are described; the presence of important nervous packages was observed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Desmodus rotundus; Feeding habits; Histology; Tongue.
Ano: 2004 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-93832004000200005
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Age Related Structural Changes in the Bulbourethral Gland of Gaddi Goat (Capra hircus) International Journal of Morphology
Archana,P; Katiyar,R. S; Sharma,D. N; Farooqui,M. M; Prakash,Ajay.
A study was conducted on the bulbourethral glands of 30 Gaddi goats, divided into three groups of 10 animals in each viz; Prepubertal (1 day old to < 18 months of age), Pubertal (18 months to < 5yrs of age) and Postpubertal (>5yrs of age). The study revealed that the glands grew at a faster speed up-to 6 months of postnatal life after which the pace slowed down considerably. Almost 10 times increase in weight and more than three times increase in all the linear mensurations were observed in prepubertal group from the time of birth. The stroma vs parenchyma ratio which was 80:20 in one-day-old kids showed three times increase in parenchyma in prepubertal animals, and the ratio became 43:57. In one day old kids luminated and non-luminated secretory...
Tipo: Journal article Palavras-chave: Bulbourethral gland; Gaddi goat; Histology; Histochemistry.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200049
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AMP-deaminase in elasmobranch fish: A comparative histochemical and enzymatic study ArchiMer
Thebault, Marie-t; Izem, Lahoucine; Leroy, Jean Paul; Gobin, Eric; Charrier, Gregory; Raffin, Jean-paul.
AMP-deaminase activity was measured in white muscle from a wide range of fish, including one cyclostome, 13 chondrosteans, and one teleost to elucidate the pattern of the AMP-deaminase activity in white muscle of fish. Compared to a mammalian (rat) muscle extract, low enzyme activities are found in the cyclostome and two elasmobranchs from two families (Scyliorhinidae, Hexanchidae). In contrast, higher AMP-deaminase activities, similar to mammals, are expressed in Squalidae, all families of skates, Chimaeridae and in the teleostean fish. We then compared AMP-deaminase activities in red and white muscles from two representative elasmobranch fish, the dogfish (Scyliorhinus canicula) and the thornback ray (Raja clavata). The fibre type composition and...
Tipo: Text Palavras-chave: White fibres; Red fibres; Muscle; Histology; Fish; Evolution; Elasmobranch; AMP deaminase.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-635.pdf
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Análisis espacio-temporal del estado nutricional de larvas de anchoíta Engraulis anchoita. Relación con las características hidrográficas y la disponibilidad de alimento OceanDocs
Díaz, M.V..
Nutritional condition studies allow the assessment of physiological state of each larva and thus, the establishment of favourable nursery areas which provide better survival and growth. In the present work, morphometrical, histological and biochemical techniques were employed to assess nutritional condition of anchovy, Engraulis anchoita, larvae captured in the Argentine Sea. Its results were complemented with oceanographic data and information about zooplankton abundances, both prey and predators of anchovy larvae. Anchovy larvae abundance and distribution would be mainly determined by physico-chemical variables. Even though no significant differences were found in larval nutritional condition among the studied areas, larval condition was slightly better...
Tipo: Theses and Dissertations Palavras-chave: Fish larvae; Nutritional requirements; Morphometry; Histology; Hydrography; Nursery grounds; Fish larvae; Nutritional requirements; Hydrography; Nursery grounds; Http://aims.fao.org/aos/agrovoc/c_2924; Http://aims.fao.org/aos/agrovoc/c_5277; Http://aims.fao.org/aos/agrovoc/c_3727; Http://aims.fao.org/aos/agrovoc/c_25438.
Ano: 2010 URL: http://hdl.handle.net/1834/3548
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Anatomical studies of in vitro organogenesis induced in leaf-derived explants of passionfruit. Repositório Alice
GLORIA, B.A. da; VIEIRA, M.L.C.; DORNELAS, M.C..
With the aim os studying the organogenesis in vitro in Passiflora edulis Sims f. flavicarpa Deg., the passionfruit, leaf-derived explants were cultured on media containing NAA or BAP and incubated either in continuous darkness or in light. The histological events leading to de novo organ formation were evaluated. Darkness induces rhizogenesis in the presence of NAA, whereas direct shoot regeneration is stimulated by light and BAP. This latter condition is recommended for passionfruit micropropagation as several adventitious shoot buds were formed from meristemoids of paranchymal origin.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Passiflora; Tissue culture; Micropropagation; Histology; Cultura de tecidos; Micropropagacao; Histologia.
Ano: 1999 URL: http://www.alice.cnptia.embrapa.br/handle/doc/101050
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Anatomy, functional morphology, evolutionary ecology and systematics of the invasive gastropod Cipangopaludina japonica (Viviparidae: Bellamyinae) Naturalis
Bocxlaer, B. Van; Strong, E.E..
The anatomy, functional morphology and evolutionary ecology of the Viviparidae, and the subfamily Bellamyinae in particular, are incompletely known. Partly as a result, genealogical relationships within the family remain poorly understood. Because of this lack in knowledge, few informed hypotheses exist on ancestral states, how differences in body plans between the subfamilies evolved, and how the peculiar biogeographic distribution patterns of viviparids have arisen. Here we document the anatomy, morphology, life history and systematics of Cipangopaludina japonica, a Japanese species that has been introduced into North America, to resolve taxonomic confusion and to improve our understanding of how form and function are related in bellamyines. Anatomical...
Tipo: Article / Letter to the editor Palavras-chave: Basal Architaenioglossa; Bellamya; Fecundity; Geometric morphometrics; Histology; Reproductive anatomy; Sexual dimorphism; 42.73.
Ano: 2016 URL: http://www.repository.naturalis.nl/record/609995
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Anomalies, Aneurysm and Histology of the Middle Cerebral Artery in Nigerian Africans International Journal of Morphology
Idowu,O. E; Shokunbi,M.T; Malomo,A. O; Akang,E. E. U.
The histology of the middle cerebral artery (MCA) in Nigerian Africans has not been previously studied. One hundred MCAs obtained at autopsy from fifty adult Nigerians were studied. The vessels were processed and stained with Ehrlich's haematoxylin and eosin, elastic Van Gieson and Masson's trichrome stains. Early branches were given off before the perforators in two middle cerebral arteries, and there was one accessory MCA, making an incidence of anomalies of 3%. No aneurysm was observed in any of the cases. The internal elastic laminas were well developed but the external elastic laminas of the vessels were poorly developed. Cióse to the bif urcations the túnica media tapered gradually and at the bif urcations, the túnica media was completely deficient...
Tipo: Journal article Palavras-chave: Anomalies; Histology; Middle cerebral artery.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400038
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Azaspiracid accumulation, detoxification and biotransformation in blue mussels (Mytilus edulis) experimentally fed Azadinium spinosum ArchiMer
Jauffrais, Thierry; Marcaillou-le Baut, Claire; Herrenknecht, Christine; Truquet, Philippe; Sechet, Veronique; Nicolau, Elodie; Tillmann, Urban; Hess, Philipp.
Azadinium spinosum (Elbrächter and Tillmann), a small marine dinoflagellate, has been recently described as a de novo producer of azaspiracid-1 and -2 (AZA1 and -2) diarrhoeic toxins. A culture of A. spinosum was established in our laboratory and optimised for pilot-scale production of this organism, to evaluate and understand AZA1 and -2 accumulation and biotransformation in blue mussels (Mytilus edulis) fed with A. spinosum. Adult mussels were continuously exposed to A. spinosum over 1 week in 160 L cylindrical conical tanks. Three different diets were tested for contamination: 5000, 10 000 cells mL−1 of A. spinosum and a mixture of 5000 cells mL−1 of A. spinosum with 5000 cells mL−1 of Isochrysis aff. galbana (T-Iso, CCAP 927/14). During the subsequent...
Tipo: Text Palavras-chave: Azaspiracid; Azadinium spinosum; Marine biotoxins; AZA; Tissue distribution; Histology; Bivalve molluscs; Liquid chromatography coupled to tandem mass spectrometry.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00088/19877/17566.pdf
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Baybean ( Canavalia rosea (Sw.) DC.); organogenesis, morphological and anatomical studies Gayana Botánica
Mohajer,Sadegh; Taha,Rosna Mat; Mohamed,Normadiha; Razak,Ummi Nur Abdul.
ABSTRACT Morphological and anatomical studies comprised leaf venation, histological analysis and epidermal peeling were carried out on Canavalia rosea (Sw.) DC.). Anatomical studies of the leaf and root determined the presence of cuticle and oil glands, arrangement of cells and the structure of vascular system. The dorsiventral leaf with pinnate venation and amphistomatic leaf with paracytic type stomata were embedded below the epidermis. Trichomes and oil glands were also observed on both adaxial and abaxial surfaces of leaf. Longitudinal sections of the callus showed the existence of meristematic cells, which could give rise to plant regeneration. In the process of organogenesis, adventitious shoots emerged from leaf and stem explants within 2 months...
Tipo: Journal article Palavras-chave: Leaf architecture; Histology; Hormone; 6-benzyladenine; Trichomes; SEM.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432017000100120
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Bioactive extracellular compounds produced by the dinoflagellate Alexandrium minutum are highly detrimental for oysters ArchiMer
Castrec, Justine; Soudant, P.; Payton, L.; Tran, D.; Miner, Philippe; Lambert, C.; Le Goic, N.; Huvet, Arnaud; Quillien, Virgile; Boullot, F.; Amzil, Zouher; Hegaret, Helene; Fabioux, C..
Blooms of the dinoflagellate Alexandrium spp., known as producers of paralytic shellfish toxins (PSTs), are regularly detected on the French coastline. PSTs accumulate into harvested shellfish species, such as the Pacific oyster Crassostrea gigas, and can cause strong disorders to consumers at high doses. The impacts of Alexandrium minutum on C. gigas have often been attributed to its production of PSTs without testing separately the effects of the bioactive extracellular compounds (BECs) with allelopathic, hemolytic, cytotoxic or ichthyotoxic properties, which can also be produced by these algae. The BECs, still uncharacterized, are excreted within the environment thereby impacting not only phytoplankton, zooplankton but also marine invertebrates and...
Tipo: Text Palavras-chave: Harmful algal bloom (HAB); Crassostrea gigas; Paralytic shellfish toxins (PST); Bioactive extracellular compounds (BEC); Histology; Behavior.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00435/54637/56042.pdf
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Biochemical and cellularchanges in Oreochromis niloticus related to the water pollution of a degraded river - doi: 10.4025/actascibiolsci.v35i3.13207 Biological Sciences
Cruz, Zilma Maria Almeida; Universidade Vila Velha; Castro, Rodrigo Roque Lesqueves de; Universidade Vila Velha; Canal, Adriana; Azevedo Júnior, Romildo Rocha; Universidade Vila Velha; Silva, Ary Gomes da; Universidade Vila Velha; Ramos, Alessandro; Universidade Vila Velha.
The effects of polluted water at three sites in the Marinho River, Brazil, on Oreochromis niloticus (Nile tilápia) were investigated using histological, hematological and biochemical approaches. Fish exposed to the impacted water demonstrated that histological changes in gills were accompanied by nuclear and micronuclei abnormalities in cells. The activity of liver and plasma biomarkers (alkaline phosphatase (ALP), acid phosphatase (ACP), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and liver glutathione S-transferase (GST)) showed an expressive change due to the. The results were also correlated with the highest levels of Cu+2, Zn+2 and Mn+2 in the water. The data of this study evidenced the importance of using a set of biomarkers to...
Palavras-chave: Aminotransferases; Phosphatases; Glutathione-S-transferase; Histology; Cell abnormality; Tilapia.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/13207
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Biología reproductiva comparada de especies de la familia Sciaenidae en aguas del Río de la Plata y Costa Bonaerense OceanDocs
Militelli, M.I..
In the Argentine Sea (SW Atlantic), particularly in the Bonaerense Coastal Zone there are two frontal zones, the waterfront area of the Rio de la Plata and the southern area of the province called El Rincón. The fauna of the Bonaerense Coastal Area has its own characteristics and in the case of fish is defined by dominant species, as is the whole of Sciaenidae family composed of seven species: white mouth croaker (Micropogonias furnieri), striped weakfish (Cynoscion guatucupa), king weakfish (Macrodon ancylodon), black drum (Pogonias cromis), Argentine croaker (Umbrina canosai), drum (Paralonchurus brasiliensis) and southern kingfish (Menticirrhus americanus). In the present work is discussed in a comparative way various aspects of the reproductive cycle...
Tipo: Theses and Dissertations Palavras-chave: Brackishwater fish; Reproduction; Spawning grounds; Coastal waters; Fronts; Environmental conditions; Histology; Reproduction; Spawning grounds; Coastal waters; Environmental conditions; Http://aims.fao.org/aos/agrovoc/c_6507; Http://aims.fao.org/aos/agrovoc/c_7277; Http://aims.fao.org/aos/agrovoc/c_25205; Http://aims.fao.org/aos/agrovoc/c_32980.
Ano: 2007 URL: http://hdl.handle.net/1834/3549
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Biologia reproductiva de la saraquita Ramnogaster arcuata (Jenyns, 1842) del estuario del Rio de la Plata. OceanDocs
Rodrigues, K.A..
The reproductive biology of Ramnogaster arcuata inhabiting waters of the Rio de la Plata estuary (Argentina - Uruguay) was studied by using macroscopic and histological analysis of ovaries. R. Arcuata specimens were collected from two research cruises of the National Institute of Fisheries Research and Development (INIDEP) carried out in June 1998 and June 1999. During these surveys, bottom salinity and temperature data were also registered. In addition, information about annual catches of this species in the Rio de la Plata, obtained from different INIDEP cruises performed during the last ten years, was analyzed. During winter, a prevalence of R. arcuata individuals was observed in the Rio de la Plata estuary. In summer, this species apparently moves...
Tipo: Theses and Dissertations Palavras-chave: Sexual reproduction; Ovaries; Fecundity; Spawning seasons; Histology; Fish eggs; Oocytes; Environmental conditions; Ovaries; Sexual reproduction; Fecundity; Oocytes; Environmental conditions; Http://aims.fao.org/aos/agrovoc/c_5456; Http://aims.fao.org/aos/agrovoc/c_26876; Http://aims.fao.org/aos/agrovoc/c_15725; Http://aims.fao.org/aos/agrovoc/c_29343; Http://aims.fao.org/aos/agrovoc/c_32980.
Ano: 2005 URL: http://hdl.handle.net/1834/1485
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