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A case of intersex occurrence in Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae) under captivity condition: a cytogenetic and morphological study Neotropical Ichthyology
Honji,Renato M.; Caneppele,Danilo; Pandolfi,Matias; Nostro,Fabiana L. Lo; Moreira,Renata G..
ABSTRACT Little is known about reproductive biology of endangered Steindachneridion parahybae , a gonochoristic teleost species inhabiting the Paraíba do Sul River Basin, and herein is the first description of intersex in S. parahybae juvenile. The normal appearance of ovaries and testes in juvenile from the same lot of breeding were also described for comparison, even as cytogenetic analysis was performed in these juveniles. One specimen was a priori classified as female due to the macroscopic characteristic of ovaries, with small yellow oocytes, without fringes (a main characteristic of catfish male), and larger than testes; however the microscopic analysis revealed the presence of ovotestes, including the complete spermatogenesis. S. parahybae had...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Histology; Karyotype; Ovary; Ovotestes; Testicles.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000400206
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A Comparative Study of Fixatives for Axolotl Blastema Tissue International Journal of Morphology
Keskin,Ilknur; Yildirim,Berna; Kolbasi,Bircan; Öztürk,Gürkan; Demircan,Turan.
Regeneration is defined as tissue renewal and functional restoration process of the damaged parts of the body after an injury. Ambystoma mexicanum, commonly named the Axolotl, is one of the unique vertebrates, which has a remarkable ability to regenerate their extremities following the amputation. Although the process of regeneration includes several periods, it can be divided into two main phases; blastema formation and dedifferentiation. In the couple of hours following the amputation, wound closure occurs by migration of epithelial cells around the amputation site followed by macrophage infiltration and dedifferentiation of cells to turn into stem cells. Accumulated stem cells form a very authentic tissue type called blastema, which is crucial for...
Tipo: Journal article Palavras-chave: Axolotl; Blastema; Fixatives; Histology.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100009
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A histological study of microsporogenesis in Tarenna gracilipes (Rubiaceae) Naturalis
Vinckier, S.; Smets, E..
We studied the microsporogenesis in Tarenna gracilipes (Hayata) Ohwi, with special attention to the mode of exine deposition and tapetum development. We based this research on light (LM), scanning electron (SEM) and transmission electron microscopic (TEM) observations of developing anthers of T. gracilipes, from the microspore mother cell stage towards anther dehiscence. Evidence is supplied that the microsporogenesis in T. gracilipes can be considered as simultaneous. Columellae, foot layer and tectum develop in a fibrillar matrix. Similar with earlier studies in Rubiaceae species, a single white line formed near the plasmalemma in the extra-apertural region. The developing endexine dilated into several white line centered lamellae at the apertures. An...
Tipo: Article / Letter to the editor Palavras-chave: Tarenna gracilipes; Rubiaceae; Microsporogenesis; Exine deposition; Tapetum development; Histology; Light microscopy; Scanning.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/407305
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A histological study on the development of the digestive system of Pseudosciaena crocea larvae and juveniles ArchiMer
Mai, Kangsen; Yu, Hairui; Ma, Hongming; Duan, Qingyuan; Gisbert, Enric; Zambonino, Jose-luis; Cahu, Chantal.
The ontogenetic development of the gut and accessory organs in large yellow croaker Pseudosciaena crocea was investigated using light microscopy from hatching up to the juvenile stage (40 days post hatch, dph). At 3 dph (mean +/- S.D., 4.1 +/- 0.1 mm total length, L-T), coinciding with the buccopharynx opening, larvae started to feed exogenously, and the gut consisted of a well-developed buccopharynx, a partially-differentiated oesophagus and an intestine divided in three regions (anterior intestine, intermediate intestine and rectum). Yolk reserves were not completely depleted at the onset of exogenous feeding, and a period of mixed nutrition was observed up to 6 dph (4.3 +/- 0.1 mm L-T) when yolk was definitively exhausted. Important morphological...
Tipo: Text Palavras-chave: Pseudosciaena crocea; Ontogenetic development; Large yellow croaker; Histology; Digestive system.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-930.pdf
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A Macroanatomical and Histological Study of the Uropygial Gland in the White Stork (Ciconia cicionia) International Journal of Morphology
Kozlu,Tolunay; Bozkurt,Yesim Akaydin; Ates,Sevinc.
The present study was aimed at the macroanatomical and histological investigation and the demonstration of the structural characteristics of the uropygial gland in the white stork. The uropygial gland of two adult male white storks constituted the material of the study. It was determined that the gland was situated subcutaneously in between the caudal aspect of the lumbosacral bone and the first coccygeal vertebrae. The gland was composed of two lobes, which displayed an oval appearance when viewed from above and resembled a water drop when observed medially. Tuft of feathers were present on the caudal margin of each lobe. The present study revealed that the uropygial gland of the white stork is a simple tubular gland, which discharges its secretion into...
Tipo: Journal article Palavras-chave: White stork; Uropygial gland; Anatomy; Histology.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300010
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A shortcut method for sexing juvenile European sea bass, Dicentrarchus labrax L. ArchiMer
Menu, Bruno; Peruzzi, Stefano; Vergnet, Alain; Vidal, Marie-odile; Chatain, Beatrice.
The objective of this study was to develop a rapid and effective method of sexing juvenile sea bass with minimum labour and material. To this end, the gonad squash mount technique was applied along with macroscopic techniques for sexing a large number of experimental fish at the age of 215-275 days post fertilization (p.f.). At this age, 90% of the 3894 fish could be unambiguously sexed by macroscopic examination of their gonads, whereas the remaining proportion was identified using squash mount preparations (10%). The accuracy of the observations was measured up to classical histology procedures. Undifferentiated fish accounted for 0.1% of the total population only. The application of the squash mount technique holds out the prospect of considerable...
Tipo: Text Palavras-chave: Histology; Squash; Juvenile; Gonads; Seabass; Dicentrarchus labrax.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-378.pdf
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Academic Achievement and Perception of Two Teaching Methods in Histology: Light Microscopy and Digital Systems International Journal of Morphology
Becerra G,Daniela; Grob L. P,Melisa; Rodríguez R,Ángel; Barker M,María José; Consiglieri L,Lucas; Ferri G,Giorgio; Sabag S,Natividad.
With the advent of digital systems, the role of the microscope as an irreplaceable instrument in the practical teaching of histology has been called into question. In this study academic performance and student perception for three learning methods was compared: digital systems, microscopy, and microscopy plus digital systems, in the muscle tissue unit of the morphology course for first-year dentistry at the Universidad de los Andes, Santiago, Chile. Ninety-five students were divided into 3 groups: Group 1: individual optical microscopy, Group 2: digital systems (one projector per room), and Group 3: microscopy plus digital systems. All participants observed the same striate muscle, cardiac striated muscle, and smooth muscle mounts. Their diagnostic...
Tipo: Journal article Palavras-chave: Medical education; Histology; Visual learning; Virtual slides; Microscopy.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300001
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Acute hepatotoxicity of Crotalus durissus terrificus (South American rattlesnake) venom in rats J. Venom. Anim. Toxins incl. Trop. Dis.
França,RF; Vieira,RP; Ferrari,EF; Souza,RA; Osorio,RAL; Prianti-Jr,ACG; Hyslop,S; Zamuner,SR; Cogo,JC; Ribeiro,W.
Venom of the South American rattlesnake, Crotalus durissus terrificus (Cdt), presents myotoxic and neurotoxic outcomes, but reports on its effects on the liver are scarce. This study examined the hepatotoxicity resulting from Cdt venom administration (100, 200 and 300 µg/kg) in male Wistar rats. Animals were studies at 3, 6, 9 and 12 hours after venom injection. The hepatotoxicity was assessed through serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (AP), gamma glutamyl transferase (GGT), bilirrubin and also by histopathological evaluation. All the different concentrations of Cdt venom resulted in increased levels of hepatic enzymes, when compared with the control group, except for the 100 µg/kg dose,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crotalus durissus terrificus; Hepatotoxicity; Histology; AST; ALT; Rattlesnake; Liver enzymes; Venom.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100007
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Acute oral toxicity and anti-inflammatory activity of hydroalcoholic extract from Lampaya medicinalis Phil in rats Biol. Res.
Morales,Glauco; Paredes,Adrián; Olivares,Alberto; Bravo,Jaime.
BACKGROUND: Algesia and inflammation are related with several pathological conditions. It is known that many drugs available for the treatment of these problems cause unwanted side effects. This study was aimed at evaluating acute toxicity and anti-inflammatory activity of Lampaya medicinalis Phil. (Verbenaceae) widely used in the folk medicine of Northern Chile against rheumatism, arthritis and body joints pain. RESULTS: Oral administration of hydroalcoholic extract (HAE) at the highest dose of 3000 mg/ Kg body weight resulted in no mortalities or evidence of significant behavioral changes. Histological examination revealed normal architecture and no significant adverse effects were observed on the liver, kidney, heart, lung or ovaries and testicles. The...
Tipo: Journal article Palavras-chave: Lampaya medicinalis; Hydroalcoholic extract; Acute oral toxicity; Histology; Hematology; Anti-inflammatory; Carrageenan.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100006
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Acute toxicity and histopathology in ornamental fish amazon bluespotted corydora (Corydoras melanistius) exposed to formalin Anais da ABC (AABC)
Santos,Rudã F.B.; Dias,Henrique M.; Fujimoto,Rodrigo Y..
The objective of this work was to evaluate the acute toxicity of formalin and histopathological effects on the Amazon ornamental fish, bluespotted coridora (Corydoras melanistius). A randomized design was used, with ten concentrations of formalin (40%) (0, 3, 6, 12, 25, 50, 100, 150, 200 and 250mg.L-1) with four replicates and five fish per container (3L) in static system for 96 hours. The moribund fish were killed and fixed in 10% formalin to proceed the histopathological analysis of gill, liver and kidney. At the end of this experiment the following mortality rates (%) were obtained in increasing order of exposure: 0, 0, 0, 0, 0, 65, 85, 100, 100 and 100%. The lethal concentration 50% (LC50-96h (I)) estimated was 50.76 mg.L-1 with regression of y =...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lethal concentration; Corydora; Formaldehyde; Toxicity; Histology; Amazon.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000400014
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Adaptaciones morfológicas de la lengua de Desmodus Rotundus Rotundus (Chiroptera: Phyllostomidae) en función de la alimentación Mastozool. neotrop.
Mollerach,Marcos I.; Mangione,Susana.
An analysis of the external morphology and histomorphology of the tongue, as well as an histochemical analysis of the glands of Desmodus rotundus, are here presented with the purpose of establishing a correlation between these structures and the feeding habits of this species. In the lingual surface they have the typical filiform papillae modified to different degrees of keratinization, and there are fungiform-like papillae in less amount. The intrinsic musculature and the presence of lynphocitary infiltration are described; the presence of important nervous packages was observed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Desmodus rotundus; Feeding habits; Histology; Tongue.
Ano: 2004 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-93832004000200005
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Age Related Structural Changes in the Bulbourethral Gland of Gaddi Goat (Capra hircus) International Journal of Morphology
Archana,P; Katiyar,R. S; Sharma,D. N; Farooqui,M. M; Prakash,Ajay.
A study was conducted on the bulbourethral glands of 30 Gaddi goats, divided into three groups of 10 animals in each viz; Prepubertal (1 day old to < 18 months of age), Pubertal (18 months to < 5yrs of age) and Postpubertal (>5yrs of age). The study revealed that the glands grew at a faster speed up-to 6 months of postnatal life after which the pace slowed down considerably. Almost 10 times increase in weight and more than three times increase in all the linear mensurations were observed in prepubertal group from the time of birth. The stroma vs parenchyma ratio which was 80:20 in one-day-old kids showed three times increase in parenchyma in prepubertal animals, and the ratio became 43:57. In one day old kids luminated and non-luminated secretory...
Tipo: Journal article Palavras-chave: Bulbourethral gland; Gaddi goat; Histology; Histochemistry.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200049
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AMP-deaminase in elasmobranch fish: A comparative histochemical and enzymatic study ArchiMer
Thebault, Marie-t; Izem, Lahoucine; Leroy, Jean Paul; Gobin, Eric; Charrier, Gregory; Raffin, Jean-paul.
AMP-deaminase activity was measured in white muscle from a wide range of fish, including one cyclostome, 13 chondrosteans, and one teleost to elucidate the pattern of the AMP-deaminase activity in white muscle of fish. Compared to a mammalian (rat) muscle extract, low enzyme activities are found in the cyclostome and two elasmobranchs from two families (Scyliorhinidae, Hexanchidae). In contrast, higher AMP-deaminase activities, similar to mammals, are expressed in Squalidae, all families of skates, Chimaeridae and in the teleostean fish. We then compared AMP-deaminase activities in red and white muscles from two representative elasmobranch fish, the dogfish (Scyliorhinus canicula) and the thornback ray (Raja clavata). The fibre type composition and...
Tipo: Text Palavras-chave: White fibres; Red fibres; Muscle; Histology; Fish; Evolution; Elasmobranch; AMP deaminase.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-635.pdf
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Análisis espacio-temporal del estado nutricional de larvas de anchoíta Engraulis anchoita. Relación con las características hidrográficas y la disponibilidad de alimento OceanDocs
Díaz, M.V..
Nutritional condition studies allow the assessment of physiological state of each larva and thus, the establishment of favourable nursery areas which provide better survival and growth. In the present work, morphometrical, histological and biochemical techniques were employed to assess nutritional condition of anchovy, Engraulis anchoita, larvae captured in the Argentine Sea. Its results were complemented with oceanographic data and information about zooplankton abundances, both prey and predators of anchovy larvae. Anchovy larvae abundance and distribution would be mainly determined by physico-chemical variables. Even though no significant differences were found in larval nutritional condition among the studied areas, larval condition was slightly better...
Tipo: Theses and Dissertations Palavras-chave: Fish larvae; Nutritional requirements; Morphometry; Histology; Hydrography; Nursery grounds; Fish larvae; Nutritional requirements; Hydrography; Nursery grounds; Http://aims.fao.org/aos/agrovoc/c_2924; Http://aims.fao.org/aos/agrovoc/c_5277; Http://aims.fao.org/aos/agrovoc/c_3727; Http://aims.fao.org/aos/agrovoc/c_25438.
Ano: 2010 URL: http://hdl.handle.net/1834/3548
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Anatomical studies of in vitro organogenesis induced in leaf-derived explants of passionfruit. Repositório Alice
GLORIA, B.A. da; VIEIRA, M.L.C.; DORNELAS, M.C..
With the aim os studying the organogenesis in vitro in Passiflora edulis Sims f. flavicarpa Deg., the passionfruit, leaf-derived explants were cultured on media containing NAA or BAP and incubated either in continuous darkness or in light. The histological events leading to de novo organ formation were evaluated. Darkness induces rhizogenesis in the presence of NAA, whereas direct shoot regeneration is stimulated by light and BAP. This latter condition is recommended for passionfruit micropropagation as several adventitious shoot buds were formed from meristemoids of paranchymal origin.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Passiflora; Tissue culture; Micropropagation; Histology; Cultura de tecidos; Micropropagacao; Histologia.
Ano: 1999 URL: http://www.alice.cnptia.embrapa.br/handle/doc/101050
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ANATOMICAL STUDIES OF IN VITRO ORGANOGENESIS INDUCED IN LEAF-DERIVED EXPLANTS OF PASSIONFRUIT PAB
GLORIA,BEATRIZ APPEZZATO DA; VIEIRA,MARIA LUCIA CARNEIRO; DORNELAS,MARCELO CARNIER.
With the aim of studying the organogenesis in vitro in Passiflora edulis Sims f. flavicarpa Deg., the passionfruit, leaf-derived explants were cultured on media containing NAA or BAP and incubated either in continuous darkness or in light. The histological events leading to de novo organ formation were evaluated. Darkness induces rhizogenesis in the presence of NAA, whereas direct shoot regeneration is stimulated by light and BAP. This latter condition is recommended for passionfruit micropropagation as several adventitious shoot buds were formed from meristemoids of parenchymal origin.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Passiflora; Tissue culture; Micropropagation; Histology.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X1999001100005
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Anatomy, functional morphology, evolutionary ecology and systematics of the invasive gastropod Cipangopaludina japonica (Viviparidae: Bellamyinae) Naturalis
Bocxlaer, B. Van; Strong, E.E..
The anatomy, functional morphology and evolutionary ecology of the Viviparidae, and the subfamily Bellamyinae in particular, are incompletely known. Partly as a result, genealogical relationships within the family remain poorly understood. Because of this lack in knowledge, few informed hypotheses exist on ancestral states, how differences in body plans between the subfamilies evolved, and how the peculiar biogeographic distribution patterns of viviparids have arisen. Here we document the anatomy, morphology, life history and systematics of Cipangopaludina japonica, a Japanese species that has been introduced into North America, to resolve taxonomic confusion and to improve our understanding of how form and function are related in bellamyines. Anatomical...
Tipo: Article / Letter to the editor Palavras-chave: Basal Architaenioglossa; Bellamya; Fecundity; Geometric morphometrics; Histology; Reproductive anatomy; Sexual dimorphism; 42.73.
Ano: 2016 URL: http://www.repository.naturalis.nl/record/609995
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Anomalies, Aneurysm and Histology of the Middle Cerebral Artery in Nigerian Africans International Journal of Morphology
Idowu,O. E; Shokunbi,M.T; Malomo,A. O; Akang,E. E. U.
The histology of the middle cerebral artery (MCA) in Nigerian Africans has not been previously studied. One hundred MCAs obtained at autopsy from fifty adult Nigerians were studied. The vessels were processed and stained with Ehrlich's haematoxylin and eosin, elastic Van Gieson and Masson's trichrome stains. Early branches were given off before the perforators in two middle cerebral arteries, and there was one accessory MCA, making an incidence of anomalies of 3%. No aneurysm was observed in any of the cases. The internal elastic laminas were well developed but the external elastic laminas of the vessels were poorly developed. Cióse to the bif urcations the túnica media tapered gradually and at the bif urcations, the túnica media was completely deficient...
Tipo: Journal article Palavras-chave: Anomalies; Histology; Middle cerebral artery.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000400038
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Anti-diabetic effects of Campomanesia xanthocarpa (Berg) leaf decoction BJPS
Vinagre,Anapaula Sommer; Rönnau,Ângela Della'Santa Rubio Origuella; Pereira,Sabrina Francisco; Silveira,Lídia Uliano da; Wiilland,Elenir de Fátima; Suyenaga,Edna Sayuri.
The objective of this research was to identify the effects of 3-week treatment of normal and streptozotocin-induced diabetic rats using a leaf decoction of Campomanesia xanthocarpa Berg. (20 g/L) on physiological, biochemical and histological parameters. Streptozotocin (STZ, 70 mg/kg in citrate buffer, pH 4.5) was administered IP to induce experimental diabetes one week prior to the treatment. STZ caused typical diabetic symptoms: polydypsia, polyuria, polyphagia, hyperglycemia, hypertriglyceridemia and histopathological modifications in the pancreas, liver and kidney. The treatment of diabetic rats using the decoction decreased blood glucose levels, inhibited hepatic glycogen loss, and prevented potential histopathological alterations in the pancreas and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Campomanesia xanthocarpa/pharmacognosy; Diabetes Mellitus/experimental study; Hypoglycemic plant; Histology; Carbohydrate/metabolism; Lipid/metabolism.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200002
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Aspectos gametogênicos e histoquímicos de Iphigenia brasiliana (Lamarck) (Bivalvia, Donacidae) da Lagoa de Itaipu, Niterói, Rio de Janeiro, Brasil Zoologia
Mesquita,Eliana de Fátima Marques de; Abreu,Marta Guimarães de; Lima,Francisco Carlos de.
Gametogenic and histochemical aspects of an edible bivalve, lphigenia brasiliana (Lamarek, 1818) from Itaipu Lagoon, Niterói, State of Rio de Janeiro, were studied. Between May 1998 and October 1999 a total of 120 clams were picked up. Samples of 10 animals were collected once a month. Specimens were fixed in Bouin's fixative for 4 hours or less and transferred to 10% formalin for 24 hours. The shell lenght has been measured with the valves lightly opened to permit the penetration of fixative solution deeply in the tissues. Transverse sections including digestive diverticula, mantle, gills, gonad and foot were obtained from the total body. After fixative treatment, tissues sections were embedded in paraffin, cut routinely at 5 µm and stained with Harris's...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gametogenic cycle; Histology; Special-staining methods; Iphigenia brasiliana; Ltaipu Lagoon; Brazil.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000200023
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