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Registros recuperados: 157
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16s-23S rDNA: polymorphisms and their use for detection and identification of Xylella fastidiosa strains BJM
Martinati,Juliana Camargo; Pacheco,Flávia Tereza Hansen; Miranda,Vitor Fernandes Oliveira de; Tsai,Siu Mui.
Strains of Xylella fastidiosa from several hosts (coffee, citrus, grape, almond, oleander, peach, plum, etc.) were characterized by analyzing the content of the nucleotide sequences of 16S-23S rDNA (coding for a small subunit ribosomal RNA) spacer region (ITS). Current methods for sequencing the ITS region yields partial sequences that do not contribute with significant information. According to this fact, new primers have been designed in order to obtain a complete sequence and facilitate the sequencing. The complete 16S-23S sequences from 08 strains were amplified through PCR using the primers designed in our lab. The 16S-23S sequences obtained were compared with 52 others sequences entries in GenBank database. The results revealed a higher level of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic diversity; 16S-23S rDNA; Intergenic spacer; Identification; Detection.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100033
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A key to the Afrotropical genera of Muscidae (Diptera) Zoologia
Couri,Márcia S..
A key to 57 of the 58 Afrotropical Muscidae (Diptera) genera is given. The previous Catalogue of Afrotropical Diptera published about 25 years ago by Adrian C. Pont was used as a taxonomic guide, to which the more recently synonyms and taxonomic changes were updated. For each genus, geographical distribution and number of valid species in this region are also included. Some morphological diagnostic characters are illustrated in order to help the use of the key.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Afrotropical Region; Identification; Taxonomy.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752007000100022
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A Linnaeus NGTM interactive key to the Lithocolletinae of North-West Europe aimed at accelerating the accumulation of reliable biodiversity data (Lepidoptera, Gracillariidae) Naturalis
Doorenweerd, C.; Haren, M.M. van; Schermer, M.; Pieterse, S.; Nieukerken, E.J. van.
We present an interactive key that is available online through any web browser without the need to install any additional software, making it an easily accessible tool for the larger public. The key can be found at http://identify.naturalis.nl/lithocolletinae. The key includes all 86 North-West European Lithocolletinae, a subfamily of smaller moths (“micro-moths”) that is commonly not treated in field guides. The user can input data on several external morphological character systems in addition to distribution, host plant and even characteristics of the larval feeding traces to reach an identification. We expect that this will enable more people to contribute with reliable observation data on this group of moths and alleviate the work-load of taxonomic...
Tipo: Article / Letter to the editor Palavras-chave: Cameraria; Phyllonorycter; Macrosaccus; Triberta; Identification; Monitoring; Conservation; Biodiversity; Leafminers.
Ano: 2014 URL: http://www.repository.naturalis.nl/record/648110
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A novel acidophilic, thermophilic iron and sulfur-oxidizing archaeon isolated from a hot spring of tengchong, yunnan, China BJM
Ding,Jiannan; Zhang,Ruiyong; Yu,Yizun; Jin,Decai; Liang,Changli; Yi,Yang; Zhu,Wei; Xia,Jinlan.
A novel thermoacidophilic iron and sulfur-oxidizing archaeon, strain YN25, was isolated from an in situ enriched acid hot spring sample collected in Yunnan, China. Cells were irregular cocci, about 0.9-1.02 µm×1.0-1.31 µm in the medium containing elemental sulfur and 1.5-2.22 µm×1.8-2.54 µm in ferrous sulfate medium. The ranges of growth and pH were 50-85 (optimum 65) and pH 1.0-6.0 (optimum 1.5-2.5). The acidophile was able to grow heterotrophically on several organic substrates, including various monosaccharides, alcohols and amino acids, though the growth on single substrate required yeast extract as growth factor. Growth occurred under aerobic conditions or via anaerobic respiration using elemental sulfur as terminal electron acceptor. Results of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Isolation; Identification; Acidianus manzaensis; Bioleaching.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200016
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A polyphasic approach for black Aspergillus identification using MALDI-TOF MS. IPB - Escola Superior Agrária
Rodrigues, Paula; Soares, Célia; Santos, Cledir; Fraga, Marcelo; Venâncio, Armando; Lima, Nelson.
The Aspergillus section Nigri is among the best studied fungi, having different commercial applications, but also causing biodeterioration of commodities and food spoilage. Although being well studied, their identification is not straightforward, and, recently, new species have been described in this section1,2,3. These new species were not only separated from their relatives in the section by morphological distinction but also from molecular point of view. The concept of species is clearly abstract and delimitations are very difficult, and often not consensual. Recently, Matrix-Assisted Laser Desorption/Ionisation Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) has been used to generate spectrum of protein masses in a range of 2000 to 20000 Da that is a...
Tipo: ConferenceObject Palavras-chave: Black aspergilli; Identification.
Ano: 2009 URL: http://hdl.handle.net/10198/3202
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A simple and reliable PCR-restriction fragment length polymorphism assay to identify Candida albicans and its closely related Candida dubliniensis BJM
Ge,Yi Ping; Wang,Le; Lu,Gui Xia; Shen,Yong Nian; Liu,Wei Da.
Candida dubliniensis is an emerging pathogen capable of causing superficial as well as systemic infections. Due to its close similarity to C. albcians, conventional methods based on phenotypic traits are not always reliable in identification of C. dubliniensis. In this study, we developed a PCR-restriction fragment length polymorphism (RFLP) assay to identify and discriminate between the two closely related species. The D1/D2 region of 28S rDNA was amplified by PCR and enzymatically digested by ApaI and BsiEI respectively. PCR products of both species were digested into two fragments by ApaI, but those of other yeast species were undigested. BsiEI cut the PCR products of C. albicans into two fragments but not those of C. dubliniensis. Thus two species were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Candida dubliniensis; PCR-restriction fragment length polymorphism; Identification; Differentiation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300004
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Acervo do herbário IAN. 1. Meliaceae Ventenat. Infoteca-e
MARTINS-DA-SILVA, R. C. V.; FERREIRA, G. C..
1998
Tipo: Documentos (INFOTECA-E) Palavras-chave: Herbário; Planta; Identificação; Banco de dados; Meliaceae Ventenat; Amazônia; Brasil; Herbaria; Plants; Identification; Databases.
Ano: 1998 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/388116
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Acervo do herbário IAN. 2. Lecythidaceae Poiteau. Infoteca-e
JESUS, K. A. S. de; FERREIRA, G. C.; MARTINS-DA-SILVA, R. C. V..
2000
Tipo: Documentos (INFOTECA-E) Palavras-chave: Herbário; Planta; Identificação; Banco de dados; Lecythidaceae; Amazônia; Brasil; Herbana; Plants; Identification; Databases.
Ano: 2000 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/392024
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Age Estimation from Ossification of the Medial Clavicular Epiphysis by Computed Tomography International Journal of Morphology
El Morsi,Doaa A.; Abo El-Atta,Hend M; ElMaadawy,Merit; Tawfik,Ahmed M; Batouty,Nihal M.
The current study intended to evaluate the accuracy of age estimation from the development of the medial clavicular epiphysis (sternal end of the clavicle) in Egyptian population using computed tomography (CT) and to develop a discriminant formula that can be used in Egyptians. The study was conducted on 142 subjects (84 males and 58 females) after taken informed consent. They were subjected to multi-slice CT on the medial end of clavicles of both sides. The results revealed that the age of non - union was seen at 8­17 years; incomplete union at 15­20 years and complete union was seen at 20 years. It is concluded that the ossification of medial clavicular end could be used for age estimation. Also CT is a good visualization tool to be used. Regression...
Tipo: Journal article Palavras-chave: Forensic science; Age estimation; Ossification status; Medial clavicular epiphysis; Sternal end of the clavicle; Identification; Computed tomography.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400038
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Alternative techniques to study characters of the genitalia in Lepidoptera Neotropical Entomology
Dias,Fernando M S; Casagrande,Mirna M; Mielke,Olaf H H.
The present note aims to describe two alternative methods for observing genitalia in Lepidoptera. The first one provides means to examine both male and female genitalia without spoiling the scales of the abdomen, preserving it attached to the thorax and aesthetically similar to an unexamined specimen. The second one provides ways of observing certain characters on the male genitalia in a non-destructive way, and does not depend on time-consuming removing and dissection of the abdomen. It is expected that the presented techniques will help on morphological studies and on identifying similar species which consistently differ in genitalic armatures.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Morphology; Identification; Valva; Uncus.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000600030
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Amostragem de plantas, preparo de amostras e remessa ao laboratório para identificação de doença. Infoteca-e
PAIVA, F. de A.; GOULART, A. C. P..
2000
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Doenca de planta; Identificacao; Laboratorio; Cerrado; Plant diseases; Identification; Laboratories.
Ano: 2000 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/243315
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An aid to the identification of the South American species of Amphisbaena (Squamata, Amphisbaenidae) Pap. Avulsos de Zool. (São Paulo)
Vanzolini,P. E..
A matrix of meristic characters is presented to orient the identification of South American Amphisbaena. Complementarily is given a checklist containing the citation of the original description, the type locality, identified, and a citation of a modern description useful to the identification.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amphisbaena; Identification.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0031-10492002001500001
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An external tag for fish: tagging effects in different fish size classes and its influence on growth performance and hematology of Lophiosilurus alexandri (Siluriformes: Pseudopimelodidae) Neotropical Ichthyology
Boaventura,Túlio P.; Gil,Viviane S. B.; Gil,Camila S. B.; Peres,Anderson M.; Luz,Ronald K..
ABSTRACT This study tested an external tag for juveniles of Lophiosilurus alexandri and measured the effects of tagging in different size classes of fishes. Experiment 1 evaluated the retention rate and influence of the tag on survival and growth of three hundred fishes divided Small, Medium and Large size classes. After 90 days of experiment the fishes had 100% survival and the tag had a higher retention rate for animals of the Large size class. Experiment 2 evaluated the tag’s influence on hematology parameters of forty-two fishes at 5, 10 and 30 days post-tagging. In this experiment both tagged and untagged animals experienced 9.4% mortality. The hematocrit was higher on the 30th day for tagged animals than for untagged animals. No difference was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catfish; Identification; Pacamã; Physiology; Repopulation; Retention.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000100214
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An illustrated key to male Actinote from Southeastern Brazil (Lepidoptera, Nymphalidae) Biota Neotropica
Francini,Ronaldo Bastos; Penz,Carla M..
In certain times of the year, Actinote butterflies can be vastly abundant in Southeastern Brazil, thus representing conspicuous denizens of our fauna. Their wing coloration allows for the recognition of five major patterns, which probably resulted from mimicry. Furthermore, examination of series of field-collected and reared specimens clearly demonstrates a high intra-specific variation in wing color pattern. In concert, inter-specific resemblance (mimicry) and intra-specific variation make it difficult to discriminate Actinote species from each other. This key aims at providing a tool for identification of male Actinote. To that effect, we provide diagnostic characters and illustrations of wings and male genitalia for 22 species. We hope that the clear...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Butterfly; Identification; Genitalia; Mimicry.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032006000100013
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Analysis of Palatal Rugae Morphology Before and After Orthodontic Treatment by a Digital Image Recognition System International Journal of Morphology
Xiu-Ping,Wu; Jian-Ning,Han; Pan,Fen; Yu-Jin,Wan; Li,Bing.
To analyze the accuracy rate of a digital image recognition system in matching the palatal rugae Morphology after orthodontic treatment, before orthodontic treatment and to provide theoretical evidence for individual identification in the field of forensic dentistry. High-resolution digital images of the palatal rugae were taken under particular conditions from each patient before and after orthodontic treatment. Features of each digital image were extracted using a digital image recognition system and included in the palatal rugae database. Using an MATLAB software system, information matching of the palatal rugae was performed. From the matching results, the accuracy rate of individual identification of orthodontic patients was 100 %, with a Euclidean...
Tipo: Journal article Palavras-chave: Forensic anthropology; Forensic stomatology; Palatal rugae; Identification; Orthodontic treatment.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200005
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Antibiotic susceptibility profile of bacilli isolated from the skin of healthy humans BJM
Tarale,Prashant; Gawande,Sonali; Jambhulkar,Vinay.
Abstract In the present work, twelve bacilli were isolated from four different regions of human skin from Bela population of Nagpur district, India. The isolated bacilli were identified by their morphological, cultural and biochemical characteristics. Seven isolates were Gram negative rods, out of which five were belong to genus Pseudomonas. Three among the five Gram positive isolates were identified as Dermabactor and the remaining two Bacillus. Their antimicrobial susceptibility profile was determined by Kirby-Bauer disc diffusion method. The isolates showed resistance to several currently used broad-spectrum antibiotics. The Dermabactor genus was resistant to vancomycin, although it was earlier reported to be susceptible. Imipenem was found to be the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibiotic resistance; Identification; Microflora; Nosocomial; Opportunistic.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401111
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Application of Palatal Rugae Morphology in Forensic Identification International Journal of Morphology
Wu,Xiu-Ping; Han,Jian-Ning; Fen,Pan; Wang,Yu-Jin; Bing,Li.
To investigate the feasibility of application of digital images of the palatal rugae in forensic identification. One hundred patients, consisting of 50 males and 50 females, who received treatment between January 2015 and June 2015 at Shanxi Medical University Stomatological Hospital, China, were included in this study. High-resolution digital image of the palatal rugae was taken from each patient using a digital SLR camera and then processed using a digital image recognition system, including noise reduction, contrast enhancement, image segmentation, feature extraction, edge detection and information matching. Using an MATLAB software system, match results and the time taken by each operator for information matching were recorded. The digital image...
Tipo: Journal article Palavras-chave: Forensic anthropology; Forensic stomatologia; Palatal rugae; Identification.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200017
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Aves migratórias ocorrentes no Pantanal: caracterização e conservação. Infoteca-e
NUNES, A. P.; TOMAS, W. M..
Periodicamente o Brasil é visitado por milhares de aves que realizam movimentos sazonais da América do Norte para a América do Sul e vice-versa (Sick 1983, Morrison et al. 1989, Chesser 1994). Dentre as aves que visitam o território nacional, destacam-se aquelas que migram com a proximidade do inverno boreal (Antas 1994). Os migrantes vêm ao Brasil à procura de locais de invernada, onde encontram alimentação farta, propiciando-lhes a continuidade do seu ciclo de vida (Telino Jr. et al. 2003). Estas aves apresentam extraordinária capacidade de percorrer longas distâncias (Hayman et al. 1986; Antas 1989; Morrison et al. 1989; Sick 1983, 1997; Azevedo Jr. et al. 2002, Alerstan et al. 2003). No período de inverno, a baixa oferta de recursos alimentares aliada...
Tipo: Documentos (INFOTECA-E) Palavras-chave: Ave; Migração; Conservação; Identificação; Pantanal; Bird; Migration; Conservation; Identification.
Ano: 2004 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/811658
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BEEF '97, PART I: REFERENCE OF 1997 BEEF COW-CALF MANAGEMENT PRACTICES AgEcon
Dargatz, David.
The NAHMS Beef '97 Study was designed to provide both participants and the industry with information on the nation's cow-calf population for education and research. The USDA's National Agricultural Statistics Service (NASS) collaborated with NAHMS to select a statistically-valid sample yielding 2,713 producers from 23 states. The 23-state target population represented 85.7 percent of U.S. beef cows on January 1, 1997, and 77.6 percent of U.S. beef operations. NASS enumerators collected data for this report via a questionnaire administered on-farm from December 30, 1996, through February 3, 1997. Contact for this paper: David Dargatz
Tipo: Report Palavras-chave: NAHMS; Beef; Cattle; Cow-calf; Labor; Identification; Weaning; Marketing; Breeding; Calving; Livestock Production/Industries.
Ano: 1997 URL: http://purl.umn.edu/32742
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BEEF, '97, PART IV: CHANGES IN THE U.S. BEEF COW-CALF INDUSTRY, 1993-1997 AgEcon
Dargatz, David.
Section 1 of this report shows demographic changes of the U.S. beef cow-calf industry from a historical perspective from data provided by the National Agricultural Statistics Service (NASS) and Census of Agriculture. Results of two NAHMS national studies in Section II complete the overview of change in the U.S. beef cow-calf industry during the 5-year period from 1993 to 1997. NAHMS, CHAPA Phase I included data collected from 2,539 cow-calf operations in the 48 continental states via telephone during the fall of 1992. Subsequent data collection was done on operations in 18 states and from producers with five or more beef cows and 50 percent or more of their calf crop born between January 1 and June 30 (spring calving) The 18 states represented 70...
Tipo: Report Palavras-chave: NAHMS; Beef; Cattle; Cow-calf; Demographics; Identification; Economics; Marketing; Weaning; Morbidity; Mortality; Nutrition; Livestock Production/Industries.
Ano: 1998 URL: http://purl.umn.edu/32755
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