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A model of chronic IgE-mediated food allergy in ovalbumin-sensitized mice BJMBR
Saldanha,J.C.S.; Gargiulo,D.L.; Silva,S.S.; Carmo-Pinto,F.H.; Andrade,M.C.; Alvarez-Leite,J.I.; Teixeira,M.M.; Cara,D.C..
Food allergy is most frequently the result of IgE-mediated hypersensitivity reactions. Here, we describe a chronic model in which some of the intestinal and systemic consequences of continuous egg white solution ingestion by ovalbumin-sensitized eight-week-old BALB/c mice, 6 animals per group, of both sexes, were investigated. There was a 20% loss of body weight that began one week after antigen exposure and persisted throughout the experiment (3 weeks). The sensitization procedure induced the production of anti-ovalbumin IgG1 and IgE, which were enhanced by oral antigen exposure (129% for IgG1 and 164% for IgE, compared to sensitization values). Intestinal changes were determined by jejunum edema at 6 h (45% Evans blue extravasation) and by a significant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Food allergy; Ovalbumin; Murine model; IgE; Hypersensitivity.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600005
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Effects of industrial cashew nut processing on anacardic acid content and allergen recognition by IgE. Repositório Alice
MATTISON, C. P.; CAVALCANTE, J. M.; GALLÃO, M. I.; BRITO, E. S. de.
bitstream/item/177370/1/ART18001.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Ácidos Anarcárdicos; Alérgeno alimentar; Processamento de comida; IgE; Allergy; Anacardic acids; Cashew; Food allergen; Caju; Alergia; Anacardium Occidentale; Food processing.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1089545
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Immunoglobulin E and systemic lupus erythematosus BJMBR
Atta,A.M.; Sousa,C.P.; Carvalho,E.M.; Sousa-Atta,M.L.B..
Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by intense polyclonal production of autoantibodies and circulating immune complexes. Some reports have associated SLE with a Th2 immune response and allergy. In the present study 21 female patients with SLE were investigated for total IgE and IgE antibodies to dust house aeroallergens by an automated enzyme-linked fluorescent assay, and were also evaluated for antinuclear IgE autoantibodies by a modified indirect immunofluorescence test using HEp-2 cells as antigen substrate. Additionally, immunocapture ELISA was used to investigate serum anti-IgE IgG autoantibodies. Serum IgE above 150 IU/ml, ranging from 152 to 609 IU/ml (median = 394 IU IgE/ml), was observed in 7 of 21 SLE...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Systemic lupus erythematosus; Antinuclear IgE antibody; Allergy; IgG anti-IgE; IgE.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000008
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Production and characterization of a monoclonal antibody against an Ascaris suum allergenic component BJMBR
Pires,R.R.; Oshiro,T.M.; Itami,D.M.; Fernandes,I.; Macedo-Soares,M.F..
Ascaris suum allergenic components (PIII) separated by gel filtration chromatography of an adult worm extract were used to immunize BALB/c mice. Popliteal lymph node cells taken from the immunized animals were fused with SP2/O myeloma cells using polyethylene glycol (MW 1450) as fusogen. The hybridomas were cultured in HAT-containing medium and cloned at limiting dilutions. Supernatants from the growing hybrids were screened by ELISA using plates coated with PIII or the A. suum crude extract. The monoclonal antibody obtained, named MAC-3 (mouse anti-A. suum allergenic component), is an IgG1 kappa mouse immunoglobulin that specifically recognizes a 29,000 molecular weight protein (called allergenic protein) with an affinity constant of 1.7 x 10(9) M-1. The...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Ascaris suum; Allergen; IgE; Monoclonal antibodies.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800009
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Restoration of adenosine deaminase, histamine, and IgE in organs of mice injected with cobra venom followed by specific treatment and reversal period J. Venom. Anim. Toxins incl. Trop. Dis.
Lipps,B. V..
Adenosine deaminase (ADA), histamine, and IgE are endogenously present in animals. Research from this laboratory reported decreased levels of these substances in organs of mice as a consequence of sub-lethal injection of Naja kaouthia venom. This research reports that decreased ADA, histamine, and IgE levels were prevented by specific treatment and prolonged recovery periods. Adult Balb/c mice injected IM with sub-lethal venom dose were divided into five groups. Group 1 were injected with PBS; Group 2 with anti-cobra venom; and Group 3 with lethal toxin neutralizing factor (LTNF). Groups 4 and 5 were treated with IM or oral synthetic LT-10. After 24 hours, mice were sacrificed and organ homogenates were assayed for ADA, histamine, and IgE. Group 1 showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cobra venom; Mouse organs; ADA; Histamine; IgE; Homeostasis.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200007
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Role of immunoglobulin E and mast cells in murine models of asthma BJMBR
Mayr,S.I.; Zuberi,R.I.; Liu,F.-T..
Immunoglobulin E (IgE) and mast cells are believed to play important roles in allergic inflammation. However, their contributions to the pathogenesis of human asthma have not been clearly established. Significant progress has been made recently in our understanding of airway inflammation and airway hyperresponsiveness through studies of murine models of asthma and genetically engineered mice. Some of the studies have provided significant insights into the role of IgE and mast cells in the allergic airway response. In these models mice are immunized systemically with soluble protein antigens and then receive an antigen challenge through the airways. Bronchoalveolar lavage fluid from mice with allergic airway inflammation contains significant amounts of IgE....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Asthma; IgE; Mast cells; Airway hyperresponsiveness.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700001
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SUB-LETHAL INJECTION OF COBRA VENOM DECREASES ADENOSINE DEAMINASE, HISTAMINE, AND IgE IN ORGANS OF MICE J. Venom. Anim. Toxins
LIPPS,B. V..
The pharmacological substances adenosine deaminase (ADA), histamine, and IgE are endogenously present in animals. They are implicated in allergy and asthma and detectable in blood serum. This research reports the presence of ADA, histamine, and IgE at varying levels in almost all major organs of mice. This research further reports that intramuscular injection of sub-lethal dose of Naja kaouthia venom disrupted homeostasis and lowered the levels of ADA, histamine, and IgE in the organs of mice. Adult Balb/C male mice were injected with a half lethal dose of the venom. The mice were sacrificed at 2, 8, and 24 hours post-injection and different organs were collected. Organs were homogenized, centrifuged, and the supernatants were assayed for ADA, histamine,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cobra venom; Balb/C mice organs; Adenosine deaminase; Histamine; IgE; Naja kaouthia.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100006
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Sub-lethal injection of honeybee venom decreased the levels of endogenously present substances in organs of mice J. Venom. Anim. Toxins
LIPPS,B. V..
Pharmacological substances such as adenosine deaminase (ADA), collagen, histamine, IgE, myoglobin, and nerve growth factor (NGF) are endogenously present in animals. Research from this laboratory reported decreased levels of ADA, histamine, IgE, and NGF in organs of mice injected with sub-lethal doses of cobra venom. The goal of this research is to observe the levels of ADA, collagen, histamine, IgE, myoglobin, and NGF in certain organs of mice injected with venom from the bee Apis mellifera. Adult Balb/c female mice IM injected with half lethal dose of bee venom were sacrificed after 2 and 8 hours for removal of organs. The homogenates of the organs were assayed by ELISA for ADA, collagen, histamine, IgE, myoglobin, and NGF using respective antisera....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Honeybee venom; Adenosine deaminase; Collagen; Histamine; IgE; Myoglobin; Nerve growth factor.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000200006
Registros recuperados: 8
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