Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 13
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
BIOPROSPECTING: A TOOL TO CONSERVE CHILEAN BRYOPHYTES Gayana Botánica
Cuvertino-Santoni,Jorge; Montenegro,Gloria.
Here, we present the current state of knowledge on the diversity of Chilean bryophytes (mosses, liverworts, and hornworts), the secondary metabolites present in these plants, and the biological activity of extracts from populations of Chilean species. Our goal is to establish the potential of these plants as a source of useful molecules for pharmaceutical and agricultural industries, thus promoting their conservation. Only 3,7% of Chilean bryophytes (55 spp.) have been analyzed using chemical characterization of their extracts (methanol, ether, ethanol, dichloromethane, acetone and hexane). Only four species from Chilean and Argentinian populations (Porella chilensis (Lehm. & Lindenb.) Trevi., Riccardiapolyclada (Mitt. ex Thurn) Hässel,...
Tipo: Journal article Palavras-chave: Mosses; Liverworts; Hornworts; Uses; Secondary metabolites; Biological activity; In vitro cultivation.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432013000100002
Imagem não selecionada

Imprime registro no formato completo
Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium Ciência Rural
Gavilan,Natália Helena; Furlan,Fernanda Cardoso; Zorz,Alex Zichner; Oliveira,Leandro Silva de; Campos,Wellington Ferreira; Brondani,Gilvano Ebling.
ABSTRACT: Cochlospermum regium roots are used in popular medicine and its extract has diverse phytochemical molecules some with antimicrobial activity, consequently exposing this specie to genetic erosion risks. Thus, the objective of this study was to develop an in vitro multiplication protocol using chemical sterilization of culture medium. Therefore, explants obtained from apical buds of C. regium seedlings were inoculated into with 0.05mg L-1 NAA and 1mg L-1 BAP sterilized by chemical agent sodium hypochlorite (NaOCl) at 0.001%, 0.003% and 0.005% of active chlorine (Cl). Autoclaved culture medium was used as control. Result showed that the contamination by bacterial at 91 days of cultivation was significantly (P<0.05) controlled by autoclaving,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Autoclaving; Cerrado biome; Active chlorine; In vitro cultivation.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900400
Imagem não selecionada

Imprime registro no formato completo
Effects of somatic embryogenesis on gene expression of cloned coffee heterozygous hybrids. Repositório Alice
OLIVEIRA, K. C. de; GUIMARÃES, P. de S.; BAZIOLI, J. M.; MARTINATI, J. C.; SANTOS, M. M. dos; PADILHA, L.; GUERREIRO-FILHO, O.; MALUF, M. P..
Cloning of superior coffee plants by somatic embryogenesis can assist breeding programs on reducing the cost and time for launch of new cultivars. This study aimed to evaluate the efficiency of this methodology for cloning coffee trees with high heterozygosity, and to gather evidence that clonal progenies are faithful copies of mother plants. Selected plants IAC1 and IAC 2 from Coffea arabica breeding populations, resistant to leaf rust and leaf miner, respectively, were cloned via indirect somatic embryogenesis. Expression of selected genes involved in biological processes potentially affected by in vitro cultivation was evaluated by quantitative analysis. Genes encoding proteins associated with maintenance of DNA integrity and control of cell cycle...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: In vitro cultivation; Differential expression; Coffea Arábica; Somaclonal variation.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113261
Imagem não selecionada

Imprime registro no formato completo
Etude d'une prolifération hémocytaire chez la moule Mytilus edulis ArchiMer
Noel, Danièle.
Hemic proliferative disease in the bay mussel, Mytilus edulis, has been studied following mortalities in Washington State (USA). The disease has been reproduced in the labortory. The establishment of this experimental model led to study tumoral characteristics of the atypic hemocytes : ultrastrure, in vitro cultivation, transplantation. The infectious etiology of this tumor has been verified. It has been hypothesized to result from a viral infection since retrovirus-like particules have been observed following induction by bromodeoxyuridine. Specific monoclonal antibodies against atypic hemocytes have been obtained and characcterized
Tipo: Text Palavras-chave: Mytilus; Mollusque; Hémocyte; Tumeur; Ulstrastructure; In vitro culture; Transplantation; Rétrovirus; Anticorps monoclonaux; Mytilus; Mollusca; Hemocyte; Tumor; Ultrastructure; In vitro cultivation; Transplantation; Retrovirus; Monoclonal antibodies.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00428/53930/55057.pdf
Imagem não selecionada

Imprime registro no formato completo
Growth curve and development of the internal calli structure of Eucalyptus camaldulensis Dehn BABT
Mendonça,Evânia Galvão; Paiva,Luciano Vilela; Stein,Vanessa Cristina; Pires,Marinês Ferreira; Santos,Breno Régis; Pereira,Fabricio José.
The objective of this work was to elucidate the growth curve of Eucalyptus camaldulensis Dehn. calli analyzing their anatomical modifications. A sigmoid aspect of the growth curve of the calli fresh matter was observed, with five different phases (lag, exponential, linear, deceleration and decline). In the lag phase, the highest growth percentage 87%, was observed, which reduced during the evaluation period to 17% in the linear phase. As for the anatomical analyses, cellular multiplications was observed during the lag and exponential phases and increase in cell size during the linear phase, promoting the calli volume growth and the establishment of the globular conformation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro cultivation; Organogenesis; Anatomy; Astra blue; Safranin; Forestry.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600012
Imagem não selecionada

Imprime registro no formato completo
In Vitro Cultivation of Blood Stream Trypomastigotes of Trypanosoma vivax without Feeder Cell Layers OAK
Hirumi, Hiroyuki; Hirumi, K.; Moloo, S. K.; Shaw, M. K..
Bloodstream trypomastigotes (BSFs) of 2 clones (IL 1392 and IL 3671) of Trypanosoma vivax were cultured without feeder layers in 3 systems. System I: metacyclic-producing cultures of T. vivax IL 1392 were initiated with BSFs derived from infected mice at 27 OC in the presence of feeder layers using TVM-1 medium. Metacyclics harvested were then transferred to flasks containing feeder cells and maintained at 34 OC using TVM-22 medium. Under such conditions, the metacyclics transformed to BSFs. Bloodstream trypomastigotes which were then transferred to new flasks, continued to grow in HMI-162 medium without feeder cells. The maximum density of the BSFs and their shortest population doubling time were 3.5 x l06 / ml and 13.5 h, respectively. System II:...
Palavras-chave: Feeder cell layers; In vitro cultivation; Trypomastigotes.
Ano: 1991 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/153
Imagem não selecionada

Imprime registro no formato completo
Light in intermediate acclimatization of in vitro germinated seedlings of Dendrobium phalaenopsis Deang Suree Ciência Rural
Sorgato,José Carlos; Rosa,Yara Brito Chaim Jardim; Soares,Jackeline Schultz; Lemes,Camila Soares Rosa; Sousa,Gisele Garcia de.
The success in micropropagation of Dendrobium phalaenopsis Deang Suree is high, but when transplanted into the greenhouse, their survival is minimal. To increase survival in production in the present study it was evaluated the effect of intermediate acclimatization for 30 days in a grow room utilizing the following luminosity conditions: 1- white fluorescent light (B) (18.9µmol m-2 s-1); 2- white fluorescent light + red fluorescent light (GRO-LUX(r)) (BV) (14.85µmol m-2 s-1); 3- red fluorescent light (GRO-LUX(r)) (V) (9.45µmol m-2 s-1) and the control plants were accommodated directly in a greenhouse (162.0µmol m-2 s-1). After this the leaves were characterized anatomically and the plants transferred to the control greenhouse. It was evaluated survival...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Orchidaceae; Floriculture; Luminosity conditions; In vitro cultivation.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000200231
Imagem não selecionada

Imprime registro no formato completo
Micropropagation of Syngonanthus elegantulus Ciência e Agrotecnologia
Pêgo,Rogério Gomes; Paiva,Patrícia Duarte de Oliveira; Paiva,Renato.
The species Syngonanthus elegantulus Ruhland is an important native plant used as cut flowers, but extractive exploration has caused the endangered status of this plant. Therefore, the objective of this study was to establish a protocol of the micropropagation of S. elegantulus. To this end, seed germination was tested in media containing different concentrations of WPM salts. For plantlet establishment, MS and WPM media were evaluated with 50 and 100% of the salt concentrations. Different the sucrose levels in the WPM medium were also evaluated. For multiplication, the plantlets were inoculated in WPM media with 0.0, 0.5, 1.0, 2.0 and 4.0 mg L-1 TDZ and 0.0, 0.5 and 1.0 mg L-1 NAA in all possible combinations. The pre-acclimatization effect was tested in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro cultivation; Floriculture; Conservation; Endangered; Plant.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000100004
Imagem não selecionada

Imprime registro no formato completo
Micropropagation protocol for Syngonanthus elegans (Bong.) Ruhland: an ornamental species Agronomy
Pêgo, Rogério Gomes; Universidade Federal de Viçosa; Paiva, Patrícia Duarte de Oliveira; Universidade Federal de Lavras; Paiva, Renato; Universidade Federal de Lavras.
The research aimed to establish a protocol of micropropagation of Syngonanthus elegans. Seed germination in WPM media containing 0%, 25%, 50%, 75% and 100% salt concentrations was evaluated. Establishment of plantlets in WPM and MS media, with 50% or 100% salt concentrations, was studied. The addition of 0, 5, 10, 15, 20, 25 and 30 g L-1of sucrose in WPM media was evaluated. The effects of TDZ (0.0; 0.5; 1.0; 2.0 and 4.0 mg L-1) and ANA (0.0; 0.5 and 1.0 mg L-1) in all possible combinations were investigated. To test acclimatization, sand, PlantmaxTM or vermiculite substrates were evaluated by direct acclimatization and pre-acclimatization. The germination of S. elegans seeds was not influenced by salt concentration in the culture media, but the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.00.00-9 floriculture; In vitro cultivation; Multiplication; Acclimatization Agronomia.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/17946
Imagem não selecionada

Imprime registro no formato completo
Mycelial growth and sporulation of Apoharknessia eucalyptorum on different culture media. Repositório Alice
GARRETT, A. T. de A.; SANTOS, A. F. dos; FIGUEIREDO FILHO, A.; TAMBARUSSI, E. V.; GONÇALVES, A. B.; GARCIA, F. A. de O..
Apoharknessia eucalyptorum foi inicialmente descrita em 2017 e identificada em folhas de Eucalyptus dunnii no Sul do Brasil. No entanto, faltam informações sobre o cultivo in-vitro para estudos complementares. No presente estudo folhas de E. dunnii foram inoculadas para demonstrar a patogenicidade de A. eucalyptorum, e o crescimento e esporulação foram avaliados em temperaturas de 15, 20 e 25°C em quatro meios de cultura: extrato de malte-ágar (MEA), batata dextrose-ágar (PDA), suco V8-ágar (V8) e feijão dextrose-ágar (BEAN), sob luz constante. Apoharknessia eucalyptorum causou manchas foliares nas folhas inoculadas. As melhores condições para o crescimento micelial foram em 25°C em PDA, BEAN e MEA. Para a esporulação as melhores condições foram a 25°C em...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Apoharknessia eucalyptorum; Características de colônia; Fungo patogênico; Cultivo in vitro; Colony characteristics; Inoculation; In vitro cultivation; Inoculação; Temperatura; Doença de Planta; Temperature.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113197
Imagem não selecionada

Imprime registro no formato completo
Organogenesis and plant regeneration of Arachis villosa Benth. (Leguminosae) through leaf culture Biocell
Fontana,María Laura; Mroginski,Luis Amado; Rey,Hebe Yolanda.
With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: &#945;-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 &#956;M thidiazuron and 4.44 &#956;M 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in Arachis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: In vitro cultivation; Leaflet explants; Micropropagation; Thidiazuron; Wild groundnut.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000300006
Imagem não selecionada

Imprime registro no formato completo
Protoplast production from napier grass and pearl millet triploid hybrids. Repositório Alice
TIMBÓ, A. L. de O.; DAVIDE, L. C.; PINTO, J. E. B. P.; PEREIRA, A. V..
2010
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Pennisetum purpureum; Pennisetum glaucum; In vitro cultivation; Interspecific hybrids; Forage.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/handle/doc/868965
Imagem não selecionada

Imprime registro no formato completo
Protoplast production from napier grass and pearl millet triploid hybrids Ciência e Agrotecnologia
Timbó,Ana Luiza de Oliveira; Davide,Lisete Chamma; Pinto,José Eduardo Brasil Pereira; Pereira,Antônio Vander.
The objective of this work was to obtain protoplasts from napier grass and pearl millet triploid hybrids as a basis for future studies on chromosomal duplication. Explants were taken from mesophyll of in vitro- and in vivo-cultured plants or from calli of two triploid hybrids (H1 and H2), which were treated with enzymatic solutions containing different concentrations of cellulase R-10 (0.5, 1.0, 1.5 and 2.0%) with an additional 0.2% macerozyme and 0.1% driselase or 1.0% pectolyase Y-23 and 0.5% hemicellulase. Enzymatic digestion was monitored once every hour for five hours. Protoplasts were obtained from in vitro and in vivo leaflets of both triploid hybrids, and in vitro leaflets were the best explant sources. The quantity of produced protoplasts varied...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pennisetum purpureum; Pennisetum glaucum; In vitro cultivation; Interspecific hybrids; Forage.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000500020
Registros recuperados: 13
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional