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Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro Arq. Bras. Med. Vet. Zootec.
Sollecito,N.V.; Pereira,E.C.M.; Grázia,J.G.V.; Neves,B.P.; Couto,B.V.R.; Andrade,V.B.; Miranda,M.S.; Silva,J.K.R.; Borges,A.M..
ABSTRACT The aim of this study was to evaluate the supplementation of embryo culture medium with antioxidant obtained from oily extract of Lippia origanoides on in vitro blastocyst development and quality. Oocytes collected from slaughterhouse ovaries were matured and fertilized in vitro following standard laboratory procedures. Zygotes were cultured in SOF medium supplemented according to the following treatments: T1 embryo culture medium without antioxidant supplementation; T2)50μM/mL Cysteamine; T3)2.5μg/mL; T4)5.0μg/mL and T5)10.0μg/mL of antioxidant obtained from oily extract of Lippia origanoides. On the seventh day of culture, the blastocysts were fixed and evaluated for apoptosis rates, number of total cell and inner cell mass cells by means of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Apoptosis; Lippia origanoides; In vitro embryo production.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300723
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