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Registros recuperados: 14
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Bovine epididymal spermatozoa treatment for in vitro fertilization: Heparin accelerates fertilization and enables a reduction in coincubation time. Repositório Alice
CUNHA, A. T. M.; CARVALHO, J. O.; GUIMARÃES, A. L. S.; LEMEID, L. O.; CAIXETA, F. M.; VIANA, J. H. M.; DODE, M. A. N..
bitstream/item/192418/1/journal.pone.0209692.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Epididymal sperm; In vitro fertilization.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1105757
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Contrasting effects of heat shock during in vitro maturation on development of in vitro-fertilized and parthenogenetic bovine embryos. Repositório Alice
CAMARGO, L. S. A.; COSTA, F. Q.; MUNK, M.; WOHLRES-VIANA, S.; SERAPIÃO, R. V.; CARVALHO, B. C.; CAMPOS JÚNIOR, P. H.; VIEIRA, A. C.; NOGUEIRA, L. A. G.; VIANA, J. H. M..
bitstream/item/203452/1/Camargo-et-al-2019-Reproduction-in-Domestic-Animals.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Activation; Embryos; Maturation; Aquaporin 3; Hsp70 Protein; Ionomycin; Heat Shock; Cleavage; Blastocyst; In vitro fertilization; Parthenogenesis; Apoptosis; Gene expression; Gametocytes; Oocytes.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113387
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Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro. Repositório Alice
CRUZ, M. H. C.; SARAIVA, N. Z.; CRUZ, J. F. da; OLIVEIRA, C. S.; COLLADO, M. del; FERNANDES, H.; CASTRO, F. C. de; GARCIA, J. M..
2014
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Embryo development; Inner cell mass; In vitro fertilization; Oocytes.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1010500
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Effect of thermal stress on the maturation of bovine oocytes, in vitro embryo production and expression of genes related to stress response. Repositório Alice
LIMA, A. C. B.; CÁCERES, M. B. S.; SILVA, A. F.; GHELLER, J. M.; SILVA, G. C.; CARDOSO, C. J. T.; PEREIRA, D. M.; NOGUEIRA, E.; SILVA, W. A. L.; OLIVEIRA, S. S.; ORTIZ, G. L.; STERZA, F. A. M..
The sirtuins are members of the histone deacetylase (HDAC) family and are involved in the regulation of transcription activation and apoptosis. In this regard, the aim of this study was to investigate the influence of different temperatures during in vitro maturation (IVM) on the developmental competence of bovine oocyte, as well as mRNA expression of sirtuins.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sirtuins; Cultura In Vitro; Temperatura; Cultura de Embrião; In vitro fertilization; Temperature; Embryo (animal).
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100168
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Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes. Repositório Alice
MUNK, M.; LADEIRA, L. O.; CARVALHO, B. C. de; CAMARGO, L. S. de A.; RAPOSO, N. R. B.; SERAPIÁO, R. V.; QUINTAO, C. C. R.; SILVA, S. R.; SOARES, J. S.; JORIO, A.; BRANDAO, H. de M..
The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Gene delivery; Pellucid zone; In vitro fertilization.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1064560
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Genetic analysis of oocyte and embryo production traits in Guzerá breed donors and their associations with age at first calving. Repositório Alice
PEREZ, B. C.; PEIXOTO, M. G. C. D.; BRUNELI, F. A. T.; RAMOS, P. V. B.; BALIEIRO, J. C. C..
Abstract: The objective of this study was to estimate variance components for oocyte and embryo production traits in Guzerá breed female donors, and investigate their associations with age at first calving (AFC). The traits analyzed were the number of viable oocytes (NOV), the number of grade I oocytes (NGI), the number of cleaved embryos (NCLV), and viable embryos produced (NEMB), and the percentages of viable oocytes (POV), grade I oocytes (PGI), cleaved embryos (PCLV), and viable embryos (PEMB). Data were obtained from 5173 ovary puncture and in vitro fertilization (IVF) sessions using 1080 Guzerá female donors of different ages, occurred from March 2005 to July 2013. Variables were log-transformed (logeX+1) prior to analysis. (Co)variance components...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bivariate analysis; Genetic parameter; In vitro fertilization; Ovum pick-up; Permanent environment; Zebu cattle.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1061463
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Germinação "in vitro" de mogno. Infoteca-e
LOPES, S. da C.; LAMEIRA, O. A.; FORTES, G. R. L.; NOGUEIRA, R. C.; LEÃO, N. V. M..
2000
Tipo: Séries anteriores (INFOTECA-E) Palavras-chave: Mogno; Germinação in vitro; Cultura de tecido; Emergência; Substrato de cultura; Swietenia macrophylla; Pará; Amazônia; Brasil; In vitro fertilization; Tissue culture; Emergence; Culture media.
Ano: 2000 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/382708
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Goat incubator: the doe as a life incubator of bovine oocytes - first step. Repositório Alice
BATISTA, R. I. T. P.; CAMARGO, L. S. de A.; SOUZA FABJAN, J. M. G.; PRATES, J. F.; TREVIZAN, J. T.; BRANDÃO, F. Z.; FONSECA, J. F. da.
Despite significant improvements in the in vitro production of cattle embryos, the suboptimal in vitro culture environment still limits the embryo quality and production. Techniques that associate the advantages of in vivo and in vitro systems, such as intrafollicular transfer of immature oocytes, have been proposed mainly to increase the embryo quality. In this context, we tried to use a goat as live incubator and associated nonsurgical embryo transfer techniques in small ruminants to perform ex situ (in vivo) maturation of bovine oocytes. For this, immature bovine cumulus-oocyte complexes (COCs) of grade 1 and 2 were randomly distributed into two groups for in vitro (IVM; n = 38) and ex situ (ESM; n = 40) maturation. The IVM was performed for a period of...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Non-surgical collection; Ovino; Ovelha; Reprodução animal; Tecnologia do embrião; Cultura In Vitro; Maturação artificial; Sheep; Ewes; In vitro fertilization; In vitro culture; Reproduction; Embryo Technology; Ova; Maturation.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1074430
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Mini-Percoll processing of domestic ruminant frozen-thawed semen dispenses the use of heparin in capacitating medium. Repositório Alice
OLIVEIRA, C. C. S.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; ALFRADIQUE, V. A. P.; CÔRTES, L. R.; BRANDÃO, F. Z..
Sperm capacitation is a prerequisite for mammal successful fertilization. Although usually a capacitating substance such as heparin is used during sheep in vitro fertilization, evidences suggest that the cryopreservation process and Percoll technique could induce spontaneous capacitation. This study aimed to compare ovine, caprine and bovine frozen-thawed sperm after mini-Percoll processing on sperm parameters, receiving or not heparin supplementation. In conclusion, frozen-thawed ovine, caprine and bovine spermatozoa processed with mini-Percoll behave similarly regarding to capacitation status and does not require heparin supplementation during in vitro incubation to achieve capacitation. [Processamento pela técnica de mini-Percoll em sêmen...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sperm capacitation; Capacitação espermática; Ovino; Caprino; Bovino; Reprodução animal; Sêmen; Heparina; Sheep; Goats; Cattle; Spermatozoa; Reproduction; In vitro fertilization; Heparin.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080820
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Propagação "in vitro" de plantas de pimenta-do-reino. Repositório Alice
LEMOS, O. F. de; MENEZES, I. C. de; SILVA, V. L. de..
1996
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Pimenta-do-reino; Reprodução vegetal; Piper nigrum; In vitro fertilization.
Ano: 1996 URL: http://www.alice.cnptia.embrapa.br/handle/doc/394874
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Situação atual da produção de embriões bovinos no Brasil e no mundo Repositório Alice
GONÇALVES, R. L. R.; VIANA, J. H. M..
bitstream/item/203454/1/Romany-RBRA-2019.pdf
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Fertilização in vitro; Pecuária bovina; Transferência de embriões; Cattle livestock; Estatística; Embryo transfer; In vitro fertilization; Statistics.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113394
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The application of reproductive technologies in the management of small ruminants genetic resources. Repositório Alice
MACHADO, R.; SALLES, H. O; SIMPLICIO, A. A..
This paper presents some applications of reproductive technologies in the management of small ruminants genetic resources. It is the importance of economics for large scale adoption of a given reproductive technique. Discusses the technical advances and hindrances.
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Fertilização in vitro; Diagnóstico de prenhez; Caprino; Ovino; Reprodução animal; Inseminação artificial; Ovulação; Ciclo estral; Transferência de embrião; Recurso genético; Parto; Indução; Sheep; Goats; Reproduction; In vitro fertilization; Ovulation; Embryo transfer; Semen; Pregnancy diagnosis; Parturition; Oestrous cycle; Animal breeding; Artificial insemination.
Ano: 1996 URL: http://www.alice.cnptia.embrapa.br/handle/doc/514875
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Use of cryopreserved spermatozoa for caprine in vitro fertilization (IVF). Repositório Alice
SIMPLICIO, A. A.; BRACKETT, B. G.; KESKINTEPE, L..
1997
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Fertilização in vitro; Caprino; Espermatozóide; Criopreservação; Goats; In vitro fertilization; Cryopreservation; Spermatozoa; Reproduction control.
Ano: 1997 URL: http://www.alice.cnptia.embrapa.br/handle/doc/514937
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ผลของการเสริม Ethidium bromide หรือ CuSO4 ในปฏิกิริยา Loop-mediated isothermal amplification (LAMP) ต่อการเพิ่มความแม่นยำในการคัดเพศตัวอ่อนโคเนื้อ Thai Agricultural
Saruttiwong Boonkong; Chainarong Navanukraw; Tossapol Moonmanee; Jiratti Thammasiri; Vilaivan Khanthusaeng; Aree Kraisoon.
The study was conducted to determine effect of EB or CS supplements in LAMP reaction on greater accuracy of sexing cattle embryos. Bovine ovaries were collected from the slaughterhouse, oocytes were aspirated from follicles then were evaluated morphology. Oocytes were cultured in TCM-199 at 38.5 ºC 24 h. For in vitro fertilization (IVF), oocytes were co-incubated with spermatozoa at 38.5 ºC 24 h. Fertilization rate was evaluated and then the embryos were in vitro cultured until morula stage. Then, embryo samples were frozen and stored at -196 ºC. Frozen embryos (n=35) were randomly extracted for total cellular DNA and amplified by PCR method. DNA samples were stored at -20 ºC. Sexing was determined by modification of LAMP method with either 1mM EB or 1M CS...
Tipo: PhysicalObject Palavras-chave: Cattle; Bovine; Oocyte; LAMP reaction; Loop-mediated isothermal amplificatio; Ethidium bromide; EB; CuSO4; CS; DNA; Sexing embryo; In vitro fertilization; IVF; โคเนื้อ; โอโอไซต์; ลักษณะทางสัณฐานวิทยา; การคัดเพศตัวอ่อน; เทคนิคการคัดเพศ; ดีเอ็นเอ; ความแม่นยำ.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5813
Registros recuperados: 14
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