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A historical perspective of embryo-related technologies in South America. Repositório Alice
VIANA, J. H. M.; FIGUEIREDO, A. C. S.; GONÇALVES, R. L. R.; SIQUEIRA, L. G. B..
bitstream/item/183783/1/fulltext.pdf
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Cattle; Cloning (animals); In vitro fertilization; Statistics; Superovulation.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1096736
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Acceptable microorganisms concentration in a semen sample for in vitro embryo production BJM
D'Angelo,Magali; Pavão,Danielle L.; Melo,Gisele Martins; Rojas,Natali; Souza,Rosimeire Joaquim; Athayde,Christiani; Galuppo,Andrea Giannotti; Pinheiro,Eliana Scarcelli.
The aim of this study was to report that the acceptable concentration of microorganisms in a semen sample for insemination may not be safe for an in vitro fertilization procedure. It seems that the semen sample should be completely germ-free, because of the excellent microorganism proliferation condition promoted by the in vitro environment.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Semen; Microorganisms; In vitro fertilization.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400031
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Aspects of energetic substrate metabolism of in vitro and in vivo bovine embryos BJMBR
de Souza,D.K.; Salles,L.P.; Rosa e Silva,A.A.M..
Although the metabolism of early bovine embryos has not been fully elucidated, several publications have addressed this important issue to improve culture conditions for cattle reproductive biotechnologies, with the ultimate goal of producing in vitro embryos similar in quality to those developing in vivo. Here, we review general aspects of bovine embryo metabolism in vitro and in vivo, and discuss the use of metabolic analysis of embryos produced in vitro to assess viability and predict a viable pregnancy after transference to the female tract.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine pregnancy rates; Embryo metabolism; Metabolism profile; In vitro fertilization; Embryo culture conditions; In vivo embryo.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300191
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Bovine epididymal spermatozoa treatment for in vitro fertilization: Heparin accelerates fertilization and enables a reduction in coincubation time. Repositório Alice
CUNHA, A. T. M.; CARVALHO, J. O.; GUIMARÃES, A. L. S.; LEMEID, L. O.; CAIXETA, F. M.; VIANA, J. H. M.; DODE, M. A. N..
bitstream/item/192418/1/journal.pone.0209692.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Epididymal sperm; In vitro fertilization.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1105757
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Contrasting effects of heat shock during in vitro maturation on development of in vitro-fertilized and parthenogenetic bovine embryos. Repositório Alice
CAMARGO, L. S. A.; COSTA, F. Q.; MUNK, M.; WOHLRES-VIANA, S.; SERAPIÃO, R. V.; CARVALHO, B. C.; CAMPOS JÚNIOR, P. H.; VIEIRA, A. C.; NOGUEIRA, L. A. G.; VIANA, J. H. M..
bitstream/item/203452/1/Camargo-et-al-2019-Reproduction-in-Domestic-Animals.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Activation; Embryos; Maturation; Aquaporin 3; Hsp70 Protein; Ionomycin; Heat Shock; Cleavage; Blastocyst; In vitro fertilization; Parthenogenesis; Apoptosis; Gene expression; Gametocytes; Oocytes.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113387
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Contrasting effects of heat shock during in vitro maturation on development of in vitro-fertilized and parthenogenetic bovine embryos. Repositório Alice
CAMARGO, L. S. A.; COSTA, F. Q.; MUNK, M.; WOHLRES-VIANA, S.; SERAPIÃO, R. V.; CARVALHO, B. C.; CAMPOS JÚNIOR, P. H.; VIEIRA, A. C.; NOGUEIRA, L. A. G.; VIANA, J. H. M..
Abstract This study investigated the influence of heat shock during in vitro maturation on embryo development following in vitro fertilization (IVF) or parthenogenesis (Part). Immature bovine cumulus?oocyte complexes were exposed to heat shock (41.0°C) during the first 12 hr of in vitro maturation (IVM), followed by 12 hr at 38.5°C. Control group consisted of in vitro maturation for 24 hr at 38.5°C. Oocytes were in vitro&#8208;fertilized or activated with ionomycin and cultured in vitro for 192 hr post&#8208;in vitro insemination or parthenogenetic activation (hpia). There was an interaction (p < .01) between temperature of IVM and method of oocyte activation (IVF or Part) for cleav&#8208;age at 48 hpia. Heat shock had a negative impact (p...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Activation; Embryos; Maturation; Aquaporin 3; Hsp70 Protein; Ionomycin; Heat Shock; Cleavage; Blastocyst; In vitro fertilization; Parthenogenesis; Apoptosis; Gene expression; Gametocytes; Oocytes.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118469
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Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro. Repositório Alice
CRUZ, M. H. C.; SARAIVA, N. Z.; CRUZ, J. F. da; OLIVEIRA, C. S.; COLLADO, M. del; FERNANDES, H.; CASTRO, F. C. de; GARCIA, J. M..
2014
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Embryo development; Inner cell mass; In vitro fertilization; Oocytes.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1010500
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Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro R. Bras. Zootec.
Cruz,Maria Helena Coelho; Saraiva,Naiara Zoccal; Cruz,Jurandir Ferreira da; Oliveira,Clara Slade; Del Collado,Maite; Fernandes,Hugo; Castro,Fernanda Cavallari de; Garcia,Joaquim Mansano.
This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryo development; Inner cell mass; In vitro fertilization; Oocytes.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000300003
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Effect of insulin-like growth factor-1 during in vitro oocyte maturation and in vitro culture of bovine embryos Arq. Bras. Med. Vet. Zootec.
Quetglas,M.D.; Coelho,L.A.; Garcia,J.M.; Oliveira Filho,E.B.; Esper,C.R..
The effects of insulin-like growth factor-I (IGF-I) on in vitro maturation (IVM) (experiment I) and on in vitro embryo development (experiment II) of bovine oocytes fertilized in vitro, were evaluated in terms of cleavage (CR), blastocyst (BR) and hatching (HR) rates. For IVM, immature cumulus-oocyte complexes were cultured in TCM-199 medium supplemented with Hepes, sodium bicarbonate, sodium pyruvate, additives, fetal calf serum (B-199 medium) and gonadotropins (14 U/ml PMSG and 7 U/ml hCG). For embryo development, the oocytes/zygotes were cultured in B-199 medium with bovine oviduct epithelial cells in suspension under silicon oil. Treatments for in vitro culture conditions for both experiments were: 1- B-199 + 200 ng/ml IGF-I; 2- B-199 + 100 ng/ml...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Cleavage rate; IGF-I; In vitro maturation; In vitro fertilization.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000200014
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Effect of thermal stress on the maturation of bovine oocytes, in vitro embryo production and expression of genes related to stress response. Repositório Alice
LIMA, A. C. B.; CÁCERES, M. B. S.; SILVA, A. F.; GHELLER, J. M.; SILVA, G. C.; CARDOSO, C. J. T.; PEREIRA, D. M.; NOGUEIRA, E.; SILVA, W. A. L.; OLIVEIRA, S. S.; ORTIZ, G. L.; STERZA, F. A. M..
The sirtuins are members of the histone deacetylase (HDAC) family and are involved in the regulation of transcription activation and apoptosis. In this regard, the aim of this study was to investigate the influence of different temperatures during in vitro maturation (IVM) on the developmental competence of bovine oocyte, as well as mRNA expression of sirtuins.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sirtuins; Cultura In Vitro; Temperatura; Cultura de Embrião; In vitro fertilization; Temperature; Embryo (animal).
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100168
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Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes. Repositório Alice
MUNK, M.; LADEIRA, L. O.; CARVALHO, B. C. de; CAMARGO, L. S. de A.; RAPOSO, N. R. B.; SERAPIÁO, R. V.; QUINTAO, C. C. R.; SILVA, S. R.; SOARES, J. S.; JORIO, A.; BRANDAO, H. de M..
The pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Gene delivery; Pellucid zone; In vitro fertilization.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1064560
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Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes BJMBR
Costa,L.O.B.; Mendes,M.C.; Ferriani,R.A.; Moura,M.D.; Reis,R.M.; Silva de Sá,M.F..
The objective of the present study was to examine the association between follicular fluid (FF) steroid concentration and oocyte maturity and fertilization rates. Seventeen infertile patients were submitted to ovulation induction with urinary human follicle-stimulating hormone, human menopausal gonadotropin and human chorionic gonadotropin (hCG). A total of 107 follicles were aspirated after hCG administration, the oocytes were analyzed for maturity and 81 of them were incubated and inseminated in vitro. Progesterone, estradiol (E2), estrone, androstenedione, and testosterone were measured in the FF. E2 and testosterone levels were significantly higher in FF containing immature oocytes (median = 618.2 and 16 ng/ml, respectively) than in FF containing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Steroid in follicular fluid; Maturity of oocytes; In vitro fertilization.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100021
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Genetic analysis of oocyte and embryo production traits in Guzerá breed donors and their associations with age at first calving. Repositório Alice
PEREZ, B. C.; PEIXOTO, M. G. C. D.; BRUNELI, F. A. T.; RAMOS, P. V. B.; BALIEIRO, J. C. C..
Abstract: The objective of this study was to estimate variance components for oocyte and embryo production traits in Guzerá breed female donors, and investigate their associations with age at first calving (AFC). The traits analyzed were the number of viable oocytes (NOV), the number of grade I oocytes (NGI), the number of cleaved embryos (NCLV), and viable embryos produced (NEMB), and the percentages of viable oocytes (POV), grade I oocytes (PGI), cleaved embryos (PCLV), and viable embryos (PEMB). Data were obtained from 5173 ovary puncture and in vitro fertilization (IVF) sessions using 1080 Guzerá female donors of different ages, occurred from March 2005 to July 2013. Variables were log-transformed (logeX+1) prior to analysis. (Co)variance components...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bivariate analysis; Genetic parameter; In vitro fertilization; Ovum pick-up; Permanent environment; Zebu cattle.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1061463
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Germinação "in vitro" de mogno. Infoteca-e
LOPES, S. da C.; LAMEIRA, O. A.; FORTES, G. R. L.; NOGUEIRA, R. C.; LEÃO, N. V. M..
2000
Tipo: Séries anteriores (INFOTECA-E) Palavras-chave: Mogno; Germinação in vitro; Cultura de tecido; Emergência; Substrato de cultura; Swietenia macrophylla; Pará; Amazônia; Brasil; In vitro fertilization; Tissue culture; Emergence; Culture media.
Ano: 2000 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/382708
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Goat incubator: the doe as a life incubator of bovine oocytes - first step. Repositório Alice
BATISTA, R. I. T. P.; CAMARGO, L. S. de A.; SOUZA FABJAN, J. M. G.; PRATES, J. F.; TREVIZAN, J. T.; BRANDÃO, F. Z.; FONSECA, J. F. da.
Despite significant improvements in the in vitro production of cattle embryos, the suboptimal in vitro culture environment still limits the embryo quality and production. Techniques that associate the advantages of in vivo and in vitro systems, such as intrafollicular transfer of immature oocytes, have been proposed mainly to increase the embryo quality. In this context, we tried to use a goat as live incubator and associated nonsurgical embryo transfer techniques in small ruminants to perform ex situ (in vivo) maturation of bovine oocytes. For this, immature bovine cumulus-oocyte complexes (COCs) of grade 1 and 2 were randomly distributed into two groups for in vitro (IVM; n = 38) and ex situ (ESM; n = 40) maturation. The IVM was performed for a period of...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Non-surgical collection; Ovino; Ovelha; Reprodução animal; Tecnologia do embrião; Cultura In Vitro; Maturação artificial; Sheep; Ewes; In vitro fertilization; In vitro culture; Reproduction; Embryo Technology; Ova; Maturation.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1074430
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Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos Scientia Agricola
Takada,Luciana; Martins Junior,Alicio; Mingoti,Gisele Zoccal; Balieiro,Julio César Carvalho; Coelho,Lia Alencar.
Melatonin (MEL) acts as a powerful scavenger of free radicals and direct gonadal responses to melatonin have been reported in the literature. Few studies, however, have evaluated the effect of MEL during in vitro maturation (IVM) on bovine embryos. This study tested the addition of MEL to maturation medium (MM) with no gonadotropins on nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos. Cumulus-oocyte complexes were aspirated from abattoir ovaries and cultured in MM (TCM-199 medium supplemented with 10% fetal calf serum - FCS) at 39ºC and 5% CO2 in air. After 24 hours of culture in MM with 0.5 µg mL-1 FSH and 5.0 µg mL-1 LH; 10-9 M MEL) or 10-9 M MEL, 0.5 µg mL-1 FSH and 5.0 µg mL-1 LH, the oocytes were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Comet assay; Embryos; Gonadotropins; In vitro fertilization; Melatonin.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000400003
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Mini-Percoll processing of domestic ruminant frozen-thawed semen dispenses the use of heparin in capacitating medium. Repositório Alice
OLIVEIRA, C. C. S.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; ALFRADIQUE, V. A. P.; CÔRTES, L. R.; BRANDÃO, F. Z..
Sperm capacitation is a prerequisite for mammal successful fertilization. Although usually a capacitating substance such as heparin is used during sheep in vitro fertilization, evidences suggest that the cryopreservation process and Percoll technique could induce spontaneous capacitation. This study aimed to compare ovine, caprine and bovine frozen-thawed sperm after mini-Percoll processing on sperm parameters, receiving or not heparin supplementation. In conclusion, frozen-thawed ovine, caprine and bovine spermatozoa processed with mini-Percoll behave similarly regarding to capacitation status and does not require heparin supplementation during in vitro incubation to achieve capacitation. [Processamento pela técnica de mini-Percoll em sêmen...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sperm capacitation; Capacitação espermática; Ovino; Caprino; Bovino; Reprodução animal; Sêmen; Heparina; Sheep; Goats; Cattle; Spermatozoa; Reproduction; In vitro fertilization; Heparin.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080820
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Oocyte aspiration and in vitro embryo production in Jersey cows with selenium-supplemented diet Arq. Bras. Med. Vet. Zootec.
Moraes,G.V.; Azevedo,J.R.; Carneiro,T.C.; Cavalieri,F.L.B.; Mataveli,M.; Rossi,R.M.; Santos,T.C..
The effects of selenium (Se) in Jersey cows' diet on the aspiration of oocytes and production of embryos in vitro were studied. Groups with five Jersey cows received 3.2mg or 9.6mg Se daily, provided in the feed concentrate. Six follicular aspirations were carried out every 15 days, using only the last 5. The oocytes were classified, and standard procedures were carried out for maturation, fertilization and cultivation. The total number of oocytes (35.11±2.65 vs 23.10±2.16) and degree 1 oocytes (11.61±2.65 vs 4.75±0.97) were higher in the group that received 9.6mg Se and the quantity of naked oocytes (3.23±0.87 vs 6.22±1.18) was lower in this group. The aspirated oocytes from the cows treated with 9.6mg Se/day resulted in higher (P<0.05) embryo...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle dairy; Antioxidant; Glutathione peroxidase; In vitro fertilization; Oocyte quality.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400001
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Propagação "in vitro" de plantas de pimenta-do-reino. Repositório Alice
LEMOS, O. F. de; MENEZES, I. C. de; SILVA, V. L. de..
1996
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Pimenta-do-reino; Reprodução vegetal; Piper nigrum; In vitro fertilization.
Ano: 1996 URL: http://www.alice.cnptia.embrapa.br/handle/doc/394874
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Simulation and economic analysis of bovine sex selection R. Bras. Zootec.
Beltrame,Renato Travassos; Quirino,Celia Raquel; Barioni,Luís Gustavo; Lima,Vera Fernanda Martins Hossepian de.
A simulation model implemented in the programming software Delphi XE® was applied to evaluate sex selection in bovine. The hypothesis under investigation was that a dynamic model with stochastic and deterministic elements could detect the sexed semen technique to minimize pregnancy cost and to determine the adequate number of recipients required for in vivo (ET) and in vitro embryo production (IVP) in the proposed scenarios. Sex selection was compared through semen sexed using flow cytometry (C1) and density gradient centrifugation techniques (C2) in ET and IVP. Sensibility analyses were used to identify the adequate number of recipients for each scenario. This number was reinserted into the model to determine the biological and financial values that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beef cattle; In vitro fertilization; Modeling; Stochastic simulation.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000500003
Registros recuperados: 25
Primeira ... 12 ... Última
 

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