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Activity of the antiarrhythmic drug amiodarone against Leishmania (L.) infantum: an in vitro and in vivo approach J. Venom. Anim. Toxins incl. Trop. Dis.
Pinto,Erika G.; Tempone,Andre G..
Abstract Background: Considering the high toxicity and limited therapies available for treating visceral leishmaniasis (VL), the drug repositioning approach represents a faster way to deliver new therapies to the market. Methods: In this study, we described for the first time the activity of a potent antiarrhythmic, amiodarone (AMD), against L. (L.)infantum and its in vitro and in vivo activity. Results: The evaluation against promastigotes has shown that amiodarone presents leishmanicidal effect against the extracellular form, with an IC50 value of 10 μM. The activity was even greater against amastigotes in comparison with promastigotes with an IC50 value of 0.5 μM. The selectivity index in relation to the intracellular form demonstrated that the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmania; Visceral leishmaniasis; Amiodarone; In vitro; In vivo.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100320
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Análise in silico e in vivo da via de isoprenóides em café. Repositório Alice
TISKI, I.; PEREIRA, L. F. P.; POT, D.; MARRACCINI, P. R.; VIEIRA, L. G. E..
Os diterpenos cafestol e caveol, presentes na fração lipídica em grãos de café, originam-se da via de síntese de isoprenóides. Estes compostos são sintetizados em todos os organismos, sendo abundantes em plantas com cerca de 10 mil componentes relatados. Apesar da diversidade de funções e estruturas todos os isoprenóides derivam de cinco comuns átomos de carbono, o isopentenil difosfato (IPP) e do isômero dimetilalil difosfato (DMAPP). Em vegetais superiores, duas vias localizadas em compartimentos intracelulares separados estão envolvidas na biossíntese de IPP e DMAPP. No citosol, IPP é derivado da via do ácido mevalônico (MVA) e no plastídeo, IPP é formado pela via do metileritritol fosfato (MEP ou não mevalonato). Com a disponibilidade das seqüências...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Coffea; Coffee; Café; Análise; Analysis; In silico; In vivo; MEP; MVA..
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/handle/doc/906049
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Antitumor activity of C-phycocyanin from Arthronema africanum (Cyanophyceae) BABT
Gardeva,Elena; Toshkova,Reneta; Yossifova,Liliya; Minkova,Kaledona; Ivanova,Natalia; Gigova,Liliana.
Pure C-phycocyanin (C-PC) was isolated from Arthronema africanumto evaluate its potential antitumor effects in vivo and in vitro. Experimental myeloid Graffi tumor in hamsters was used as a model. The cell proliferation assay showed that C-PC treatment, at concentration of 100 µg mL-1 for 24 h, significantly inhibited the growth of Graffi tumor cells (51.4% viability). Agarose gel electrophoresis of the genomic DNA of treated cells displayed time-and concentration-dependent fragmentation pattern, typical for apoptosis. Apoptotic process was related to the increase in cellular manganese and copper/zinc superoxide dismutases and glutathione reductase activities, coupled with a low catalase activity. In vivo C-PC administration (5.0 mg kg-1 body weight)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antitumor activity; Arthronema africanum; In vivo; In vitro; C-pycocyanin; Myeloid Graffi tumor.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500675
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Atividade anti-helmíntica de Cocos nucifera L. Repositório Alice
COSTA, C. T. C.; OLIVEIRA, L. M. B.; CAMURÇA-VASCONCELOS, A. L. F.; VIEIRA, L. da S.; BEVILÁQUA, C. M. L..
O controle de nematóides gastrintestinais é realizado com anti-helmínticos, mas o desenvolvimento de populações resistentes exigiu a pesquisa de novas alternativas tais como as plantas medicinais. Existem relatos populares da atividade antiparasitária do fruto de Cocos nucifera L. Desta forma a avaliação da atividade anti-helmíntica do Liquido da Casca do Coco Verde (LCCV) e dos extratos butanólico e acetato de etila do LCCV foi realizada inicialmente através de testes de atividade ovicida e larvicida sobre Haemonchus contortus; a toxicidade aguda em camundongos foi calculada para estabelecer a dose dos testes in vivo; também foram realizados estudo fitoquímico e quantificação de taninos dos extratos e finalmente a eficácia anti-helmíntica dos extratos foi...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Ovino; Parasito animal; Nematóide gastrintestinal; Tratamento anti-helmíntico; Cocos nucifera; Líquido da casca do coco verde; LCCV; In vivo; In vitro; Haemonchus contortus; Tanino; Fitoterapia; Brasil; Ceará.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/534581
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Avaliacao de fungicidas in vitro e in vivo no controle de Botryodiplodia theobromae Pat., na regiao do Submedio Sao Francisco. Repositório Alice
LIMA, J. A. S.; TAVARES, S. C. C. de H..
Este trabalho teve como objetivo avaliar a eficiência" in vitro" e u in vivo" dos fungicidas (Benomyl - 100 g, Thiophanate methyl - 100 g, Tebuconazole - 100 g, Thiabendazole - 100 g, Prochloraz - 100 ml, Prochloraz - 150 ml, Carbendazin - 100 ml, Carbendazin - 150 ml, Carbendazin + Prochloraz - 50+50 ml, Pyrimethanil - 250 ml, Pyrimethanil + Prochloraz - 150+150 ml, para 100 L de água) no controle do fungo Botryodiplodia theobromae Pat., agente causal da morte descendente em mangueira.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Avaliacao in vitro; In vivo; Controle; Submedio Sao Francisco; Evaluation; Control; Botryodiplodia Theobromae; Fungicida; Fungicides; Brazil.
Ano: 1999 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/133816
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Avaliação de métodos laboratoriais para estimar a digestibilidade e o valor energético de dietas para ruminantes Arq. Bras. Med. Vet. Zootec.
Silveira,M.F.; Kozloski,G.V.; Mesquita,F.R.; Farenzena,R.; Senger,C.C.D.; Brondani,I.L..
Foi avaliada a acurácia dos métodos laboratoriais para estimar a digestibilidade e o valor energético de dietas para bovinos de corte. As dietas experimentais foram isonitrogenadas e compostas por silagem de milho e 25, 40, 55 ou 70% de concentrado. Os valores de digestibilidade medidos in vivo foram comparados com os obtidos nos ensaios in situ, in vitro e in vitro/gases, e com valores estimados a partir de equações matemáticas baseadas na composição química das dietas. O ensaio in vivo foi realizado com quatro bovinos em delineamento em quadrado latino 4×4. Quatro animais foram usados para o ensaio in situ e quatro corridas foram realizadas para os ensaios in vitro. Não houve interação significativa de dietas versus métodos. As taxas de degradação...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ruminante; Digestibilidade; Equações; In situ; In vitro; In vivo.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000200021
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Decreased mitochondrial metabolic requirements in fasting animals carry an oxidative cost ArchiMer
Salin, Karine; Villasevil, Eugenia M.; Anderson, Graeme J.; Auer, Sonya K.; Selman, Colin; Hartley, Richard C.; Mullen, William; Chinopoulos, Christos; Metcalfe, Neil B..
Many animals experience periods of food shortage in their natural environment. It has been hypothesised that the metabolic responses of animals to naturally‐occurring periods of food deprivation may have long‐term negative impacts on their subsequent life‐history. In particular, reductions in energy requirements in response to fasting may help preserve limited resources but potentially come at a cost of increased oxidative stress. However, little is known about this trade‐off since studies of energy metabolism are generally conducted separately from those of oxidative stress. Using a novel approach that combines measurements of mitochondrial function with in vivo levels of hydrogen peroxide (H2O2) in brown trout (Salmo trutta), we show here that fasting...
Tipo: Text Palavras-chave: High-resolution respirometry; In vivo; Liver atrophy; MitoB probe; Mitochondrial respiratory state.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00449/56022/57540.pdf
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Evaluación in vivo de la actividad antimalárica de 25 plantas provenientes de una Reserva de Conservación Biológica de Costa Rica RChHN
CHINCHILLA-CARMONA,MISAEL; VALERIO-CAMPOS,IDALIA; SÁNCHEZ-PORRAS,RONALD; MORA-CHAVES,VÍCTOR; BAGNARELLO-MADRIGAL,VANESSA; MARTÍNEZ-ESQUIVEL,LAURA; GONZÁLEZ-PANIAGUA,ANTONIETA; VANEGAS,JUAN CARLOS.
Se realizó una evaluación in vivo de la actividad antimalárica de las hojas, flores, frutos, corteza y raíz de 25 plantas de la Reserva Biológica Alberto Manuel Brenes (REBAMB), situada en San Ramón, Alajuela, Costa Rica. Las plantas estudiadas fueron Aphelandra aurantiaca (Scheidw.) Lindl., Aphelandra tridentata Hemsl. (Acanthaceae), Xanthosoma undipes (K. Koch & C.D. Bouché) K. Koch. (Araceae), Iriartea deltoidea Ruiz & Pav. (Arecaceae), Neurolaena lobata (L.) Cass. (Asteraceae), Lonchocarpus pentaphyllus (Poir.) Kunth ex DC., Pterocarpus hayesii Hemsl., Senna papillosa (Britton & Rose) H.S. Irwin & Barneby., Cinnamomum chavarrianum (Hammel) Kosterm. (Fabaceae), Nectandra membranacea (Sw.) Griseb., Persea povedae W.C....
Tipo: Journal article Palavras-chave: Antimaláricos; Costa Rica; In vivo; Plantas; Plasmodium berghei.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2011000100009
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Evaluation of inhibitory effect of methylene blue against Babesia and Theileria parasites OAK
Tuvshintulga, B.; Sivakumar, T.; Salama, A.A.; Yokoyama, N.; Igarashi, I; 五十嵐, 郁男; 横山, 直明.
Methylene blue, the first fully synthetic drug, has been widely used in medical treatments. A few decades ago, this drug was used as an antimalarial agent. In this study, the in vitro inhibitory effect of methylene blue on Babesia bovis, B. bigemina, B. caballi, and Theileria equi (B. equi) and the in vivo inhibitory effect on B. microti were evaluated. Methylene blue significantly inhibited the growth of B. bovis, B. caballi, and T. equi at a 0.1 μM concentration, while B. bigemina was significantly inhibited at 0.01 μM, on day 3 of cultivation. The half maximal inhibitory concentrations (IC50) of methylene blue against B. bovis, B. bigemina, B. caballi, and T. equi were calculated as 0.83±0.02, 0.68±0.09, 0.54±0.14, and 0.49±0.06 μM, respectively. The...
Palavras-chave: Methylene blue; Babesia; Theileria; In vitro; In vivo.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4242
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Evaluation of inhibitory effects of dipyridamole against bovine and equine piroplasmosis OAK
Tuvshintulga, B.; Sivakumar, T.; Yokoyama, N.; Igarashi, I..
Dipyridamole, an antiplatelet drug used for the secondary prevention and treatment of stroke, also has antiplasmodial activity and enhances the activity of chloroquine. We evaluated the inhibitory effects of dipyridamole on the growths of Babesia and Theileria parasites. The growth of B. bovis and T. equi was significantly inhibited at a 25 μM concentration of dipyridamole, while B. bigemina and B. caballi were significantly inhibited at 50 μM and 1 μM concentrations of dipyridamole, respectively, on day 3 of cultivation. The half maximal inhibitory concentration (IC50) of dipyridamole was calculated at 39.5±4.5, 26.3±10.9, 13.2±3.6, and 23.5±0.5 μM on the growth of B. bovis, B. bigemina, B. caballi, and T. equi, respectively. In a viability assay, B....
Palavras-chave: Dipyridamole; Babesia; Theileria; In vitro; In vivo.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4497
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Expression and Localization of Olfactory Receptor AcerOr1 from Chinese Honey Bee, Apis cerana cerana (Hymenoptera, Apidae) BABT
Guo,Lina.
ABSTRACT Olfactory receptors are essential for recognition and detection of odor in honeybees. Although we have cloned and characterized the sequence of olfactory receptor AcerOr1 before, the tissue distribution and location of this gene in the nurse and the forager worker Apis cerana cerana were not very clear. To further investigate this information of AcerOr1 gene, we analyzed its expression and localization. The results showed that AcerOr1 mRNA was predominantly expressed in the antennae of nurse and forager bees, while the AcerOr1 protein was predominant in thorax, and its expression in antennae was higher in forager than in nurse. IHC revealed that AcerOr1 mainly localized in the olfactory neurons of the antennae. In addition, the staining intensity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apis cerana cerana; Olfactory receptor; AcerOr1; Expression; Localization; In vivo.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100440
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Fungos associados a podridao penduncular em mangueira irrigada no submedio Sao Francisco e eficiencia de fungicidas in vitro e in vivo. Repositório Alice
MOREIRA, W.A.; BARBOSA, F.R.; PAULA, F.R. de; MENEZES, M..
2000
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Manga; Irrigacao; Doenca; Podridao peduncular; Fungo; Fungicida in vitro; In vivo; Cylindrocladium sp; Mangoes; Irrigation; Diseases; Fungi; In vitro; In vivo fungicides.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/134044
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Identification of differentially expressed genes of Xanthomonas axonopodis pv. citri by representational difference analysis of cDNA Genet. Mol. Biol.
Mehta,Angela; Rosato,Yoko B..
Xanthomonas axonopodis pv. citri is a phytopathogenic bacterium responsible for citrus canker, a serious disease which causes severe losses in citriculture around the world. In this study we report the differential expression of X. axonopodis pv. citri in response to specific treatments by using Representational Difference Analysis of cDNA (cDNA RDA). cDNAs from X. axonopodis pv. citri cultured in the presence of leaf extract of the host plant (Citrus sinensis), in vivo, as well as in the complex medium were hybridized against cDNA of the bacterium grown in the minimal medium. Sequencing of the difference products obtained after the second and third hybridizations revealed a total of 37 distinct genes identified by homology searches in the genome of X....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus canker; Differential expression; Leaf extract; In vivo.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100024
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In vivo effects of metaldehyde on Pacific oyster, Crassostrea gigas: comparing hemocyte parameters in two oyster families. ArchiMer
Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan.
Pollutants via run-off into the ocean represent a potential threat to marine organisms, especially bivalves such as oysters living in coastal environments. These organisms filter large volumes of seawater and may accumulate contaminants within their tissues. Pesticide contamination in water could have a direct or indirect toxic action on tissues or cells and could induce alteration of immune system. Bivalve immunity is mainly supported by hemocytes and participates directly by phagocytosis to eliminate pathogens. Some studies have shown that pesticides can reduce immune defences and/or modify genomes in vertebrates and invertebrates. Metaldehyde is used to kill slugs, snails and other terrestrial gastropods. Although metaldehyde has been detected in...
Tipo: Text Palavras-chave: Immunity; Hemocytes; Metaldehyde; Pacific oyster; Flow cytometry; In vivo; Genetic diversity.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00195/30602/29059.pdf
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In vivo inhibitory effect of suberoylanilide hydroxamic acid combined with sorafenib on human hepatocellular carcinoma cells BJPS
Hao,Dalin; Deng,Fang; Shi,Hong; Wang,Hongsheng; Xiao,Fubin; Sun,Chengxue; Xu,Yansong; Li,Peng.
The present study aimed to investigate the in vivo inhibitory effect of histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) combined with sorafenib on human hepatocellular carcinoma HCCLM3 cells. The nude mice transplanted with HCCLM3 cells were randomly divided into control, SAHA, sorafenib and SAHA+sorafenib groups. The nude mice in the later 3 groups were intragastrically administrated with SAHA (10 mg·kg-1·day-1), sorafenib (10 mg·kg-1·day-1) and SAHA (10 mg·kg-1·day-1) combined with sorafenib (10 mg·kg-1·day-1), respectively, for successive 20 days. Finally, the inhibition rate of tumor was measured. The expressions of MEK1/2, p-ERK1/2, Cyclin D1, Bcl-2, Bax, p53, MMP-2, MMP-9 and uPA in tumor tissues were determined. Results showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Suberoylanilide hydroxamic acid; HCCLM3; Inhibitory; In vivo.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100528
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Intrafollicular transfer of fresh and vitrified immature bovine oocytes. Repositório Alice
SPRÍCIGO, J. F. W.; SENA NETTO, S. B.; MUTERLLE, C. V.; RODRIGUES, S. de A. D.; LEME, L. O.; GUIMARÃES, A. L.; CAIXETA, F. M. C.; FRANCO, M. M.; PIVATO, I.; DODE, M. A. N..
bitstream/item/148637/1/1-s2.0-S0093691X16302977-main.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Follicular injection; In vivo; In vitro; Blastocyst.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1054484
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Intraluteal Release of Prostaglandin F2α and E2 During Corpora Lutea Development in the Cow OAK
KOBAYASHI, Shu-ichi; ACOSTA, Tomas J.; HAYASHI, Kanako; BERISHA, Bajram; OZAWA, Takahiro; OHTANI, Masayuki; SCHAMS, Dieter; MIYAMOTO, Akio; 宮本, 明夫.
It is well known that prostaglandin (PG) F2α and PGE2 are actively produced by bovine early corpus luteum (CL) at higher levels than in the CL of later stages. However, there is no in vivo information about the local secretion of PGs within bovine developing CL so far. Thus, the objective of the present study was to determine in detail the real-time changes in PGF2α and PGE2 secretion within developing CL. A microdialysis system (MDS) was surgically implanted into the newly formed CL of 6 cows on Day 3 after GnRH administration (about 1.5 days after ovulation) to induce superovulation following FSH treatment. Both PGF2α and PGE2 release were high during the first 24 h within developing CL but decreased thereafter. These profiles were well reflected in the...
Palavras-chave: PGF2α; PGE2; Developing CL; Cow; In vivo.
Ano: 2002 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2974
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Multiplicación clonal in vivo e in vitro de la especie forestal nativa Aniba perutilis Hemsl. Acta Agron. (Palmira)
Delgado García,Lina Marcela; Hoyos Sánchez,Rodrigo Alberto.
Se desarrolló una metodología para el establecimiento in vitro e in vivo de Aniba perutilis (comino crespo), árbol colombiano en peligro crítico de extinción. En la primera etapa, se sometieron semillas de este árbol a la acción de los desinfectantes NaOCl, HgCl2 y AgNO3 para su germinación aséptica. Para inducir la formación de brotes se empleó el medio WPM y se evaluó la acción de las fitohormonas BAP, KIN, AIA, ANA y AG3 en diferentes concentraciones. Para la etapa de enraizamiento se emplearon ANA, AIB, y los medios de cultivo MS y WPM, ambos con sales reducidas a la mitad y combinados con diferentes concentraciones de AIA y ANA. En la etapa de multiplicación in vivo se emplearon plántulas establecidas en vivero para evaluar tres tamaños de explantes,...
Tipo: Journal article Palavras-chave: Comino crespo; Establecimiento in vitro; In vivo; Mini-esquejes; Recalcitrancia.
Ano: 2016 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122016000200013
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Response of seeds and pollen of Onobrychis viciifolia and Onobrychis oxyodonta var. armena to NaCl stress Scientia Agricola
Beyaz,Ramazan; Kaya,Gamze; Cocu,Sati; Sancak,Cengiz.
Sainfoin (Onobrychis viciifolia Scop.) is an important forage legume crop with 52 species adapted to dry and poor soils in Turkey, but little is known about the effects of salinity on germination and seedling growth in arid and semiarid regions suffering from salinity problem. The seeds and pollen of two species of sainfoin O. viciifolia and O. oxyodonta var. armena (Syn: O. armena) were exposed to 0, 5, 10, 20 and 30 dS m-1 of NaCl under in vivo and in vitro conditions and evaluated for germination under salt stress by comparing germination percentage, mean germination time, root and shoot length, fresh and dry seedling weight and dry matter. Increased salinity levels generally resulted in decrease in all traits except time to germination, dry seedling...
Tipo: Info:eu-repo/semantics/article Palavras-chave: In vivo; In vitro; Sainfoin; Salinity; Germination.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400013
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Seasonal changes in carbohydrate metabolism and its relationship with summer mortality of Pacific oyster Crassostrea gigas (Thunberg) in Marennes-Ole'ron bay (France) ArchiMer
Soletchnik, Patrick; Faury, Nicole; Goulletquer, Philippe.
This paper investigates the biological responses of Crassostrea gigas under traditional culture conditions on a mudflat in Marennes-Oleron bay. Summer mortality has been regularly observed in recent years in oysters reared using "on bottom" culture conditions. The present study attempts to provide a better understanding of the mortality phenomenon through biological parameters. Classical 'field parameters' such as mortality and growth rates, and quality index (dry meat weight/ dry shell weight x 1000) were monitored. Additional parameters, as biochemical composition of oyster meat and glucose incorporation capacity, were included as potential new bioindicators. The work highlighted a critical timing (May-June) preceding the summer mortality and...
Tipo: Text Palavras-chave: Marennes Oleron bay; Glycogenolysis; In vivo; Glycogen incorporation; Maturation; Summer mortality; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1116.pdf
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