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Anatomical characteristics and enzymes of the sucrose metabolism and their relationship with latex yield in the rubber tree (Hevea brasiliensis Muell. Arg.) Braz. J. Plant Physiol.
Mesquita,Alessandro Carlos; Oliveira,Luiz Edson Mota de; Mazzafera,Paulo; Delú-Filho,Nelson.
Rubber production is dependent on the availability of carbohydrates in the tissues engaged in latex synthesis. In this study three rubber tree clones were used to study the protein profile, the activities of invertase and sucrose synthase in the latex and anatomical characteristics of the bark, as well as to establish a possible relationship with latex production. The clones used in the study, RRIM 600, GT 1 and Fx2261, differed in latex productivity, from high to low in this order. Although present, few differences were observed in the latex protein profile obtained by denaturing electrophoresis. The activity of neutral invertase was higher than the acidic isoform, and both activities were higher in the most productive clones RRIM 600 and GT 1. The same...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hevea brasiliensis; Invertase; Lacticiferous vessels; Latex; Rubber; Sucrose synthase.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000200002
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Characterization of an Alternative Baking Strain of Saccharomyces cerevisiae Isolated from Fermented Cherry Fruits by the Analysis of SUC2 Gene OAK
ODA, Yuji; MIKUMO, Dai; TAJIMA, Kanako; YAMAUCHI, Hiroaki; 小田, 有二.
The yeast strain AK 46 was isolated from dough containing fermented cherry fruits and identified as Saccharomyces cerevisiae. The nucleotide sequence of the rDNA spacer region from this strain coincided with those from the yeasts used for the production of whisky, wine, and bread. In a comparison with six baking strains, strain AK 46 possessed less leavening ability in dough with and without addition of 5% sucrose (based on the weight of flour) but a high ability in sweet dough containing 30% sucrose. The activities of the two enzymes α-glucosidase and invertase, which are related to dough fermentation, were lower than those in the other strains. A baking test showed that strain AK 46 was applicable for breadmaking using the straight dough and sponge dough...
Palavras-chave: Baker's yeast; Leavening; Dough; SUC gene; Invertase.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2963
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Crescimento de plântulas do milho 'Saracura' e atividade de alfa-amilase e invertases associados ao aumento da tolerância ao alagamento exercido pelo cálcio exógeno Bragantia
Fries,Daniela Deitos; Alves,José Donizeti; Delú Filho,Nelson; Magalhães,Paulo César; Goulart,Patrícia de Fátima Pereira; Magalhães,Marcelo Murad.
Objetivou-se avaliar o crescimento da plântula e o metabolismo de carboidratos associados ao aumento da tolerância à hipoxia exercido pela presença do cálcio no período de germinação e/ou alagamento de plântulas de milho com diferentes idades. O experimento foi desenvolvido na Universidade Federal de Lavras, MG, em 2002. Cariopses de milho da variedade 'Saracura' (BRS-4154) foram germinadas em água ou solução de CaCl2. Após dois e quatro dias, as plântulas foram submetidas ao alagamento em tubos de PVC com tampão (com ou sem CaCl2) por três dias, sendo então avaliadas a sobrevivência, massa seca e as características bioquímicas. O cálcio aumentou a sobrevivência ao alagamento de plântulas com quatro dias, entretanto, não influenciou naquelas com dois dias....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Invertase; Alfa-amilase; Cálcio; Hipoxia.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052007000100001
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EFEITO DA ASSOCIAÇÃO DE PECTINASE, INVERTASE E GLICOSE ISOMERASE NA QUALIDADE DO SUCO DE BANANA Ciênc. Tecnol. Aliment.
CARDOSO,Marisa H.; JACKIX,Marisa N.H.; MENEZES,Hilary C.; GONÇALVES,Elisabeth B.; MARQUES,Simone V.B..
O efeito do tratamento em que se associou as enzimas comerciais: 0,03 % v/p de pectinase (Clarex) a 0,6 % v/p de invertase (Invertase-S) e 0,5 % p/p de glicose-isomerase (Taka-sweet) sobre purê de banana (Musa cavendishii), em condições amenas de hidrólise (40o C, 15 min.) foi observado e comparado com o efeito de outros três tratamentos enzimáticos: 0,03 % v/p de pectinase (Clarex); 0,03 % v/p de pectinase (Clarex) associada à 0,6 % v/p de invertase (Invertase-S); e 0,03 % v/p de pectinase (Sigma) associada a 0,03 % v/p de celulase (Sigma), visando determinar a qualidade representada por um conjunto de propriedades físicas, fisico-químicas, químicas, microbiológicas e sensoriais dos sucos de banana obtidos. Essas propriedades não diferiram...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pectinase; Celulase; Invertase; Glicose isomerase; Suco de banana; Qualidade.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20611998000300005
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Effect of raffinose and ultrasound pulses on invertase release by free and immobilized Saccharomyces cerevisiae in loofa (Luffa cylindrica) sponge BABT
Marques,Leila Larisa Medeiros; Buzato,João Batista; Celligoi,Maria Antonia Pedrine Colabone.
This study investigated the effect of raffinose and ultrasound pulses on invertase release from free S. cerevisiae and S. cerevisiae immobilized in Luffa cylindrica. The free cell culture was submitted to 2% raffinose pulse and irradiated for 2 minutes at 0.12 and 0.46 h-1 dilution rates. The immobilized cell culture was submitted to raffinose pulse and irradiated for 1, 2 and 4 minutes, at 0.10 h-1 dilution rate. In immobilized cells, the raffinose pulse increased the invertase activity from 5.38 to 7.27 U/mg. Ultrasound application in free cell culture at the 0.12 h-1 dilution rate gave the best results. The activity varied from 25.08 to 29.38 U/mg while the increase in immobilized cells was from 5.22 to 9.70 U/mg when sonicated for two minutes. These...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Invertase; Luffa cylindrica; Raffinose; Saccharomyces cerevisiae; Ultrasound.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700003
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Hydrolysis of galacto-oligosaccharides in soy molasses by α -galactosidases and invertase from Aspergillus terreus BABT
Reis,Angélica Pataro; Guimarães,Valéria Monteze; Ferreira,Joana Gasperazzo; Queiroz,José Humberto de; Oliveira,Maria Goreti Almeida; Falkoski,Daniel Luciano; Almeida,Maíra Nicolau de; Rezende,Sebastião Tavares de.
Two α -galactosidase (P1 and P2) and one invertase present in the culture of Aspergillus terreus grown on wheat straw for 168 h at 28ºC were partially purified by gel filtration and hydrophobic interaction chromatographies. Optimum pH and temperatures for P1, P2 and invertase preparations were 4.5-5.0, 5.5 and 4.0 and 60, 55 and 65ºC, respectively. The K M app for ρ -nitrophenyl-α -D-galactopyranoside were 1.32 mM and 0.72 mM for P1 and P2, respectively, while the K M app value for invertase, using sacarose as a substrate was 15.66 mM. Enzyme preparations P1 and P2 maintained their activities after pre-incubation for 3 h at 50ºC and invertase maintained about 90% after 6 h at 55 ºC. P1 and P2 presented different inhibition sensitivities by Ag+,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus terreus; Galactooligosaccharides; Α-galactosidase; Invertase; Soy molasses.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300029
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Identifying yeast isolated from spoiled peach puree and assessment of its batch culture for invertase production Ciênc. Tecnol. Aliment.
FERREIRA,Marcela Vega; AVILA,Tariani Lemos de; KUHN,Claudio Rafael; TORALLES,Ricardo Peraça; ROMBALDI,César Valmor.
Abstract The identification of yeasts isolated from spoiled Jubileu peach puree using the API 20C AUX method and a commercial yeast as witness were studied. Subsequently, the yeast’s growth potential using two batch culture treatments were performed to evaluate number of colonies (N), reducing sugar concentration (RS), free-invertase (FI), and culture-invertase activity (CI). Stock cultures were maintained on potato dextrose agar (PDA) slants at 4 °C and pH 5 for later use for batch-culture (150 rpm) at 30°C for 24 h, then they were stored at 4 °C for subsequent invertase extraction. The FI extract was obtained using NaHCO3 as autolysis agent, and CI activity was determined on the supernatant after batch-cultured centrifugation. The activity was followed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spoiled peach puree; Invertase; Saccharomyces cerevisiae; Autolysis.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000400701
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Invertase, glucose oxidase and catalase for converting sucrose to fructose and gluconic acid through batch and membrane-continuous reactors BJPS
Silva,Aline Ramos da; Tomotani,Ester Junko; Vitolo,Michele.
Conversion of sucrose into fructose and gluconic acid using invertase, glucose oxidase and catalase was studied by discontinuous (sequential or simultaneous addition of the enzymes) and continuous (simultaneous addition of the enzymes in a 100 kDa-ultrafiltration membrane reactor) processes. The following parameters were varied: concentration of enzymes, initial concentration of substrates (sucrose and glucose), pH, temperature and feeding rate (for continuous process). The highest yield of conversion (100%) was attained through the discontinuous (batch) process carried out at pH 4.5 and 37 ºC by the sequential addition of invertase (14.3 U), glucose oxidase (10,000 U) and catalase (59,000 U).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sucrose/conversion; Fructose; Gluconic acid; Invertase; Glucose oxidase; Catalase.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200022
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Production of thermostable invertases by Aspergillus caespitosus under submerged or solid state fermentation using agroindustrial residues as carbon source BJM
Alegre,Ana Cláudia Paiva; Polizeli,Maria de Lourdes Teixeira de Moraes; Terenzi,Héctor Francisco; Jorge,João Atílio; Guimarães,Luis Henrique Souza.
The filamentous fungus Aspergillus caespitosus was a good producer of intracellular and extracellular invertases under submerged (SbmF) or solid-state fermentation (SSF), using agroindustrial residues, such as wheat bran, as carbon source. The production of extracellular enzyme under SSF at 30ºC, for 72h, was enhanced using SR salt solution (1:1, w/v) to humidify the substrate. The extracellular activity under SSF using wheat bran was around 5.5-fold higher than that obtained in SbmF (Khanna medium) with the same carbon source. However, the production of enzyme with wheat bran plus oat meal was 2.2-fold higher than wheat bran isolated. The enzymatic production was affected by supplementation with nitrogen and phosphate sources. The addition of glucose in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus caespitosus; ²-D-fructofuranosidase; Invertase; Solid-state fermentation; Submerged fermentation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300025
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Production of α-Galactosidase by Aspergillus oryzae through solid-state fermentation and its application in soymilk Galactooligosaccharide hydrolysis BABT
Kapnoor,Shankar; Mulimani,Veerappa Hanumanth.
α-Galactosidase was produced by Aspergillus oryzae on red gram plant waste-wheat bran based media in solid-state fermentation (SSF). Optimum temperature for α-galactosidase production was 35 0C and upto 4 cm of bed height of substrate had no inhibitory effect on enzyme production. Hydrolysis of galactooligosaccharides in soymilk was carried out by α-galactosidase. Optimum temperature and pH for the hydrolysis of raffinose and stachyose of soymilk were 55(0)C and 5.2-6.2, respectively. The enzymatic treatment for 3 h completely removed the raffinose oligosaccharides in soymilk. Crude extract also showed considerable amount of invertase activity.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus oryzae; Α-galactosidase; SSF; Soymilk; Invertase; HPLC analysis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100026
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Purification and characterization of β-Fructosidase with inulinase activity from Aspergillus niger - 245 BABT
Cruz,Vinícius D'Arcadia; Belote,Juliana Gisele; Dorta,Claudia; Santos,Luíza Helena Oliveira dos; Andriolo,Cláudia Regina; Khenayfes,Marcelo de Oliveira; Cruz,Rubens.
Aspergillus niger - 245, a strain isolated from soil samples showed good β-fructosidase activity when inoculated in medium formulated with dahlia extract tubers. The enzyme was purified by precipitation in ammonium sulphate and percolated in DEAE-Sephadex A-50 and CM-cellulose columns, witch showed a single peack in all the purification steps, maintaining the I/S ratio between 0.32 to, 0.39. Optimum pH for inulinase activity (I) was between 4.0 - 4.5 and for invertase activity (S) between 2.5 and 5.0. The optimum temperature was 60O.C for both activities and no loss in activity was observed when it was maintained at this temperature for 30 min. The Km value was 1.44 and 5.0, respectively, for I and S and Vm value 10.48 and 30.55, respectively. The I...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inulinase; Invertase; Β-fructosidase; Inulin; Fructose syrup; Aspergillus niger.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000300003
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Sucrose hydrolysis by invertase using a membrane reactor: effect of membrane cut-off on enzyme performance BJPS
Addezio,Francesco Di; Yoriyaz,Ester Junko; Cantarella,Maria; Vitolo,Michele.
Sucrose hydrolysis by invertase [EC.3.2.1.26] produces inverted sugar syrup, an ingredient mainly used in the food industry. To properly catalyze hydrolysis, the enzyme should be reused after this reaction. It is advisable to maintain constant activity over a considerable period. Thus, sucrose hydrolysis was performed in a membrane bioreactor - a continuously stirred tank reactor coupled with an ultrafiltration membrane (UFM) which provides good diffusion and high activity per unit volume. Molecular weight cut-off for soluble invertase UFMs was up to 100kDa. This study focused on the role of UFM invertase cut-off as it is the main element in the process. We demonstrated that both the cut-off and chemical nature of the UFM affected specific invertase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Invertase; Membrane Reactor; Sucrose/hydrolysis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000200257
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Sucrose hydrolysis catalyzed by auto-immobilized invertase into intact cells of Cladosporium cladosporioides Electron. J. Biotechnol.
Santana de Almeida,Ana Cláudia; Costa de Araújo,Luciares; Mendes Costa,Andressa; Moraes de Abreu,César Augusto; Gomes de Andrade Lima,Maria Alice; Perez Fernandez Palha,Maria de Los Angeles.
The enzyme known as invertase (E.C. 3.2.1.26 - β-D-fructofuranosidase) catalyzes the sucrose hydrolysis producing an equimolar mixture of glucose and fructose named inverted sugar. The fungus Cladosporium cladosporioides has invertase as its constituent. Hence, its use as a natural immobilized support for the invertase produces interesting results for the enzyme. The present work has the objective of determining the optimum operational conditions of auto-immobilized invertase, as well as its kinetic parameters (K M and Vmax). A complete 2³ factorial planning was done for the evaluation of such parameters. Temperature, pH and agitation level were the studied variables. The hydrolysis percentage was the monitored result. Batch tests in optimum...
Tipo: Journal article Palavras-chave: Cladosporium cladosporioides; Factorial planning; Invertase.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100009
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Synthesis of sorbitol by Zymomonas mobilis under high osmotic pressure BJM
Barros,Márcio de; Celligoi,Maria Antonia Pedrine Colabone.
The bacterium Zymomonas mobilis presents potential for sorbitol production when grown in culture medium with high sugar concentration. Sorbitol is produced and accumulated in the periplasma of the bacterium to protect the cells from the harmful effects of high osmotic pressure that results from the action of invertase on sucrose. The conversion of sucrose into glucose and fructose increases the osmolarity of the medium. However, an excessive increase in the osmotic pressure may decrease the sorbitol production. In this work Saccharomyces cerevisiae invertase was added two media containing sucrose 200 and 300 g.L-1. Sorbitol production in sucrose at 200 g.L-1 was 42.35 and 38.42 g.L-1, with and without the invertase treatment, respectively. In the culture...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zymomonas mobilis; Invertase; Osmotic pressure; Sorbitol.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300023
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Trehalose accumulation, invertase activity and physiological characteristics of yeasts isolated from 24 h fermentative cycles during the production of artisanal Brazilian cachaça BJM
Pataro,Carla; Guerra,Juliana B.; Gomes,Fátima C.O.; Neves,Maria J.; Pimentel,Patrícia F.; Rosa,Carlos A..
Trehalose accumulation, invertase activity and physiological characteristics of 86 yeast isolates from short fermentative cycles during the production of cachaça in three artisanal distilleries of the State of Minas Gerais were studied. Among these isolates, 70% were able to grow at temperatures between 40 and 42ºC. Only Saccharomyces cerevisiae isolates were able to grow over 40ºC. Lower temperatures (<40ºC) favoured the growth of yeasts such as Candida parapsilosis-like, C. maltosa-like, Kloeckera japonica, S. exiguus and C. bombicola-like. The isolates from all three distilleries were ethanol tolerant, produced invertase, and accumulate trehalose in the presence of glucose. The strains isolated from distillery A presented more resistance to ethanol...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharomyces cerevisiae; Cachaça; Trehalose; Invertase; Fermentation.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300003
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Variação ao longo do dia da atividade de enzimas do catabolismo de sacarose em plântulas de Hymenaea courbaril L. durante a mobilização do xiloglucano de reserva Rev. Bras. Bot.
Molle,Fábio Reis Dalle; Brandão,Aline Dias; Tiné,Marco Aurélio Silva.
Sementes de Hymenaea courbaril L. possuem um polissacarídeo de reserva que é mobilizado após a germinação, quando a primeira folha da planta já é fotossinteticamente ativa. No momento da mobilização das reservas, a plântula precisa coordenar duas fontes de carboidratos: a fotossíntese e a mobilização das reservas. Ambos geram sacarose como forma de exportação de carbono. Para entender a alocação de recursos na plântula, portanto, é necessário avaliar o catabolismo de sacarose nos órgãos. Neste trabalho foram analisados os carboidratos de baixo peso, quantificada a atividade da sacarose sintase e das três isoformas de invertase nos diferentes órgãos de plântulas de H. courbaril ao longo de um dia. As dosagens foram feitas no período de mobilização do...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cotilédones; Germinação; Invertase; Sacarose sintase; Semente.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042009000100014
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Variação intra-anual dos teores foliares de carboidratos e atividade de invertases em videiras no Vale do rio São Francisco. Infoteca-e
DANTAS, B. F.; RIBEIRO, L. de S..
O pólo de agricultura irrigada do Vale do Rio São Francisco a presenta um crescente aumento na produção de uvas para vinificação. Entre os vinhos finos ali produzidos, destaca-se aquele obtido da cv. Petite Syrah, que se adaptou bem às condições climáticas da região. Pouco se conhece, no entanto, sobre o metabolismo de carboidratos das videiras nessa região. O objetivo deste trabalho foi avaliar os teores de açúcarese de amido, bem como a atividade de invertases, durante dois ciclos de produção consecutivos. O experimento foi realizado na Embrapa Semi-Árido e na Vitivinícola Santa Maria, localizadas, respectivamente, em Petrolina e em Lagoa Grande - PE. Semanalmente, de janeiro a dezembro de 2003, foram coletadas folhas para análise dos teores de açúcares...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Videira; Variação; Vale do São Francisco.; Carboidrato; Invertase; Uva..
Ano: 2005 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/156975
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Variação sazonal de algumas características nutricionais e bioquímicas relacionadas com a produção de látex em clones de seringueira [Hevea brasiliensis (Wild.) Muell. Arg.)], em Lavras-MG Ciência e Agrotecnologia
Melo,Renato Figueiredo; Oliveira,Luiz Edson Mota de; Mesquita,Alessandro Carlos; Delú Filho,Nelson.
Objetivou-se com este trabalho avaliar os efeitos das variações climáticas sazonais sobre a produção de látex, a partir de parâmetros relacionados ao fluxo e regeneração de látex e da caracterização bioquímica dos tecidos fonte e dreno, com base nas atividades das enzimas invertase neutra e sintase da sacarose (SuSy). Foram usados folíolos centrais de folhas completamente expandidas de clones de seringueira (RRIM 600, FX 2261 e GT 1) nos meses de dezembro de 2001 e julho de 2002, plantados no Setor de Fisiologia Vegetal da Universidade Federal de Lavras (UFLA). Os dados de produção foram obtidos nos respectivos meses, por meio de sangrias no sistema S ½ (D2/D3), além da utilização de borracha seca em estufa para análises minerais. Pelos dados de produção,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Seringueira; Látex; Invertase; Sintase da sacarose.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000600015
Registros recuperados: 18
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