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Registros recuperados: 19
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A reassessment of the phytogeographic characterization of Temperate Pacific South America RChHN
SANTELICES,BERNABE; MENESES,ISABEL.
In 1980, a study of geographic distribution patterns and geographic affinities of benthic algae of temperate Pacific South America (5-55 ºS) provided a first characterization of these coasts. High endemism and limited floristic exchange with the Tropical Pacific and with islands in the South Pacific at various distances from the continent were two outstanding features of the marine flora. A partial blockage of species exchange partially accounted for a peculiar latitudinal pattern of species richness that increases with increasing latitudes. In contrast, there was reduced species richness in comparison with other climatically equivalent regions that have contact with more effective routes of migration. Over the last two decades, more than 30 taxonomic and...
Tipo: Journal article Palavras-chave: Benthic phytogeography; Isolation; Latitudinal patterns; Seaweeds; Temperate South America.
Ano: 2000 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2000000400005
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Aislamiento y caracterización de Streptococcus bovis y estudio inicial para el aislamiento de su bacteriófago Colegio de Postgraduados
López Garrido, Serafín Jacobo.
El objetivo del presente estudio fue obtener un cultivo puro de la bacteria ruminal Streptococcus bovis a partir de líquido ruminal de ovinos alimentados con una dieta alta en sorgo (70 % en base seca). Además, se analizó su actividad fermentativa (producción de AGV y lactato) y el proceso de liofilizado como método de conservación, y se realizaron ensayos para expresar su bacteriófago. Para el aislamiento se usó un medio comercial para Streptococcus spp. (Merck) preparado bajo anaerobiosis. Para la identificación se usó el sistema API 20 Strep, reacción catalasa, morfología celular y tinción de Gram. Con la prueba API 20 Strep se comprobó que la bacteria aislada pertenece a la especie S. bovis. Esta bacteria tiene forma esférica a ovoide, de 0.8...
Tipo: Tesis Palavras-chave: Rumen; Streptococcus bovis; Aislamiento; Iiofilización; Bacteriófago; Doctorado; Ganadería; Isolation; Lyophilization; Bacteriophage.
Ano: 2008 URL: http://hdl.handle.net/10521/1402
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Banana Supply Chains in Indonesia and Australia: Surviving Isolation from End markets AgEcon
Singgih, Shinta; Woods, Elizabeth.
Two banana supply chains (BSC), one in Indonesia (BSC1) and one in Australia (BSC2), are described based on case study research conducted in 2002. BSC1 sources bananas from small farmers for distribution through traditional markets in Jakarta. BSC2 includes farmers supplying to both major and independent retailers in Sydney through a wholesale agency with some farmer ownership. Farmers in both chains live in relative isolation from the main markets for their bananas. Prices for their bananas depend on the market prices in the city, upon which they have little control. This paper focuses on how farmers removed from key markets, deal with buyers to receive fair prices. In both chains, most transactions between farmers and their buyers were completed through...
Tipo: Conference Paper or Presentation Palavras-chave: Farmers; Banana supply chain; Isolation; Trust.; Crop Production/Industries; Industrial Organization.
Ano: 2003 URL: http://purl.umn.edu/24336
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Characterization of a highly thermostable alkaline phosphatase from the euryarchaeon Pyrococcus abyssi ArchiMer
Zappa, Sebastien; Rolland, Jean-luc; Flament, Didier; Gueguen, Yannick; Boudrant, Joseph; Dietrich, Jacques.
This work reports the first isolation and characterization of an alkaline phosphatase (AP) from a hyperthermophilic archaeon. An AP gene from Pyrococcus abyssi, a euryarchaeon isolated from a deep-sea hydrothermal vent, was cloned and the enzyme expressed in Escherichia coli. Analysis of the sequence showed conservation of the active site and structural elements of the E. coli AP. The recombinant AP was purified and characterized. Monomeric and homodimeric active forms were detected, with a monomer molecular mass of 54 kDa. Apparent optimum pH and temperature were estimated at 11.0 and 70 degreesC, respectively. Thus far, P. abyssi AP has been demonstrated to be the most thermostable AP, with half-lives at 100 and 105 degreesC of 18 and 5 h, respectively....
Tipo: Text Palavras-chave: Escherichia coli; Characterization; Isolation; Gene; Alkaline phosphatase; Pyrococcus abyssi; Archaeon.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-1268.pdf
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Comunicação interpessoal em três assentamentos de reforma agrária de Unaí-Minas Gerais. Infoteca-e
ROCHA, F. E. de C.; ZOBY, J. L. F.; XAVIER, J. H. V.; GASTAL, M. L..
ABSTRACT: A research project on farming community development has been carried out by Embrapa Cerrados (Savannah Agricultural Research Center of the Brazilian Agricultural Research Corporation) in three Agrarian Reform Settlement Projects (PA): PA Jibóia, PA Paraíso and PA Santa Clara Furadinho, in Unaí country, Minas Gerais State, Brazil. At its initial phase, there was a diagnose and survey of information about the interpersonal interaction among group members. The objective of this article is to discuss the applicability of Moreno Sociometry in mapping the interpersonal relations between the land reform settlers to subsidize development of farming communities. initially, farmers were interviewed and requested to respond to three questions related to...
Tipo: Documentos (INFOTECA-E) Palavras-chave: Assentamento; Reforma agrária; Comunicação; Pequeno produtor; Relação interpessoal; Liderança; Isolamento; Interação; Afinidade; Sociometria; Análise; Rural settlement; Agrarian reform; Communication; Small farmers; Interpessoal relation; Leadership; Isolation; Sociometry; Affinity; Interaction; Analysis.
Ano: 2003 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/568179
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Cultivating the uncultured: limits, advances and future challenges ArchiMer
Alain, Karine; Querellou, Joel.
Since the invention of the Petri dish, there have been continuous efforts to improve efficiency in microbial cultivation. These efforts were devoted to the attainment for diverse growth conditions, simulation of in situ conditions and achievement of high-throughput rates. As a result, prokaryotes catalysing novel redox reactions as well as representatives of abundant, but not-yet cultured taxa, were isolated. Significant insights into microbial physiology have been made by studying the small number of prokaryotes already cultured. However, despite these numerous breakthroughs, microbial cultivation is still a low-throughput process. The main hindrance to cultivation is likely due to the prevailing lack of knowledge on targeted species. In this review, we...
Tipo: Text Palavras-chave: High throughput cultivation; Cell cell communication; Microbial community; Metabolism; Isolation; Culture.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6820.pdf
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Endophytic bacterium isolated of hosts present in contaminated areas by mercury in Pantanal mato-grossense. Repositório Alice
MELLO, I. S.; PIETRO-SOUZA, W.; LIOTTI, R. G.; PEREIRA, J. M. C.; BICHARA, K. P. A.; SILVA, G. F.; ALMEIDA, E. G.; SOARES, M. A..
The mercury is a contaminant in gold mining in the city of Poconé. This heavy metal is a toxic in different organisms and affect the human by bioaccumulation process causing a serie of cronic diseases. Mercury can be easily absorbed by plants and be accumulated in the human body through the food chain. The hypothesis is microorganism that is in association with plants, that survive in contaminated areas with mercury, provide greater resistance to contaminant and possibly an increased frequency of colonization. In this way, the aim of this study was to isolate endophytic bacterium that exhibit resistance to mercury.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Isolation; Tolerance; Contaminant.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1027548
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Folyami hajózás és a magyarországi gabonaexport AgEcon
Szilvassy, Zoltan.
Az árutermelésre áttért mezőgazdaság közel másfél évszázada alatt bebizonyosodott, hogy a megtermelt terményfelesleg az ország gazdaságföldrajzi „bezártsága” miatt nem versenyképes a tengeri kijutással rendelkező országok gabonájával szemben. Atengeri kikötőhöz vezető olcsó vízi utat máig sem sikerült megteremteni, és a magyar gabona jelenleg is csak a világpiaci árnál alacsonyabb áron értékesíthető. Afolyók vízjárástól független hajózhatósága azok belépcsőzésével valósítható meg. ------------------------------------------- Over the one and a half centuries since agriculture shifted from self-sufficiency to producing marketable goods, it has been proven that the surplus produce – due to the country’s geographical isolation – cannot compete against the...
Tipo: Journal Article Palavras-chave: Gazdaságföldrajz; Bezártság; Belvízi hajózás; Vízlépcsők; Eco-geography; Isolation; Inland navigation; Weirs; Agricultural and Food Policy; International Relations/Trade.
Ano: 2009 URL: http://purl.umn.edu/92479
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Islands: Palaeontology, Geology and Tectonics Naturalis
Donovan, S.K..
The intention of this Lyell Meeting is to bring together tectonicists, geologists, palaeontologists and geomorphologists to discuss the intersection of their specialisms in the geology of islands.
Tipo: Article / Letter to the editor Palavras-chave: Lyell meetings; Islands; Geology; Palaeontology; Tectonics; Evolution; Isolation; 38.20; 38.37; 38.22.
Ano: 2011 URL: http://www.repository.naturalis.nl/record/428038
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Isolamento e caracterizacao de bacilos da Regiao Norte do Brasil e avaliacao da patogenicidade contra dipteros e lepidopteros. Repositório Alice
SILVA, S.F.; CAETANO, R.C.P.S.; TOSTES, A.N.; FARIAS, J.M; DIAS, J.M.C.S..
1996
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Bacilo; Isolamento; Diptera; Lepidoptera; Caracterizacao; Brasil; Regiao Norte %% Bacillus; Isolation; Characterization; Brazil; North region.
Ano: 1996 URL: http://www.alice.cnptia.embrapa.br/handle/doc/171623
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Isolation and Analysis of Recombinant EPSP synthases from Microbial Pathogens for Drug Design Purpose Thai Agricultural
Vaithanomsat, P.; Brown, K..
Palavras-chave: Isolation; Analysis; Recombinant.
Ano: 1461 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5025
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Isolation and biological characterization of a basic phospholipase A2 from Bothrops jararacussu snake venom Biocell
Maruñak,S.L.; Leiva,L; Garcia Denegri,M.E.; Teibler,P; Acosta De Pérez,O.
A phospholipase A2 has been isolated from Bothrops jararacussu venom from snakes that inhabit the northeast region of Argentina. The present study describes in vivo and in vitro biological activities of phospholipase A2 from B. jararacussu as well as isolation details of its. Venom was obtained by milking of adult snakes which were housing in wood reptile cages of varying dimensions in heated (20-30ºC) rooms. Snakes received a weekly diet of mice and water was available ad libitum for drinking and soaking. The enzyme was purified by gel filtration on a Sephadex G-75 column followed by ion exchange chromatography on a SP-Sephadex C25 column. The major peak belonging to proteins was retained in the cation exchanger and then eluted using a concentration...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Snake venom; Bothrops jararacussu; Phospholipase A2; Isolation.
Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300001
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Isolation and survival of Yersinia enterocolitica in ice cream at different pH values, stored at -18°c BJM
Pederiva,Norma B. Barbini de; Guzmán,Ana M. Stefanini de.
The presence of Yersinia enterocolitica was investigated in 203 samples of industrial (123) and non-industrial ice cream (80). Two Y. enterocolitica strains were isolated from non-industrial ice cream, which suggests the possibility of post-manufacturing contamination. One strain was typed as B:1A, O: 3,50,51; lis Xz, while the other one was biotyped as: B:1A but not serologically typed. Survival of Y. enterocolitica was investigated by inoculating nine samples of industrially manufactured ice cream to contain 20 CFU/ml of Y. enterocolitica and stored at -18°C for 480 days. The inoculated samples were classified into three different groups according to their pH (Group 1: pH 4-5; Group 2: pH 5-6 and Group 3: pH 6-7). Viability was determined by a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yersinia; Isolation; Survival; Ice cream; PH.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300005
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Isolation of mutants of Trichoderma harzianum with enhanced b-glucosidase production with ability to inhibit and the plant pathogen Sclerotinia sclerotiorum. Repositório Alice
MELO, I. S. de; FAULL, J.L.; GRAEME-COOK, K.A.; FRIGHETTO, R. T. S.; SILVA, C.M.M.S..
Antagonism of Trichoderma spp. against other microrganisms in general operates by exolysis, breaking down the walls of fungal pathogens. Trichoderma species seem to be the best source of extracellular cellulase that can solubilize highly ordered cellulose. Cell walls of sclerotia of slerotiorum usually contain chitin and b-glucans. Such sclerotia may be vulnerable to attack by many microrganisms. The objective of this research was the isolation of improved strains for b-glucosidase production. The new biotypes obtained from a wild strain of T. harzianum were isolated via UV light in solid medium containing wheat-bran. Two mutants produced significant amounts of extra cellular cellulase activity with b-glucosidase activity. The percentage of reduction of...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Isolation; Mutants; Trichoderma harzianum; Enhanced; B-glucosidase; Production; Pathogen; Sclerotinia sclerotiorum.
Ano: 1996 URL: http://www.alice.cnptia.embrapa.br/handle/doc/12605
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Isolation of simple sequence repeats from groundnut Electron. J. Biotechnol.
Tang Wang,Chuan; Dao Yang,Xin; Xu Chen,Dian; Lin Yu,Shan; Zhen Liu,Guang; Yi Tang,Yue; Zhi Xu,Jian.
SSRs have proved to be the most powerful tool for variety identification in groundnut of similar origin, and have much potential in genetic and breeding studies. To facilitate SSR discovery in groundnut, we proposed a highly simplified SSR isolation protocol based on multiple enzyme digestion/ligation, mixed biotin-labeled probes and streptavidin coated magnetic beads hybridization capture strategy. Of the 272 colonies randomly picked for sequencing, 119 were found to have unique SSR inserts
Tipo: Journal article Palavras-chave: Groundnut; Isolation; Simple sequence repeat.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300015
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METHOD OF DEFINING THE ISOLATION PARAMETERS IN 1000 WATT SYMMETRIC ELECTRICITY NETWORK AgEcon
Sidorov, Alexander; Utegulov, Bolatbek; Utegulov, Arman; Begentaev, Bagustar; Uahitova, Aygul; Amurgaliev, Samat.
The authors define parameters for isolation three-phase electric network to provide safety conditions for personnel working at open-operated coal deposits. The authors conclude the technique provides satisfactory accuracy of the parameters’ defining, as well as simplicity and safety of coal mining works with electric network having pressure up to 1000 Watt.
Tipo: Journal Article Palavras-chave: Isolation; Electric network; Electric pressure; Isolated neutral; Research and Development/Tech Change/Emerging Technologies.
Ano: 2009 URL: http://purl.umn.edu/94561
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Ocupação de manchas florestais no Pantanal por duas espécies de roedores: Clyomys laticeps e Thrichomys pachyurus. Repositório Alice
CAMILO, A. R.; TOMAS, W. M.; SOUZA, F. L. de; BOLZAN, A.; COELHO, A. G..
A fragmentação e a perda de habitat afetam de diferentes maneiras as comunidades biológicas. os efeitos variam muito de espécie para espécie, com algumas sendo extintas enquanto outras são beneficiadas. Tendo em vista o mosaico vegetacional que caracteriza a paisagem no Pantanal, o presente estudo relacionou o tamanho e o isolamento médio das manchas florestais à probabilidade de ocupação por duas espécies de roedores: Clyomys laticeps (Thomas, 1841) e Thrichomys pachyurus (Wagner, 1845).
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Fragmentação; Isolamento; Pequenos mamíferos; Probabilidade de ocupação; Fragmentation; Isolation; Small mammals; Occupancy.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/handle/doc/869029
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Primeiro isolamento de Actinobacillus seminis em um caprino no município de Patos, Paraíba, Brasil MV&Z
Santos, Fabrine A.; Azevedo, Sergio S.; Mota, Rinaldo Aparecido; Kim, Pommy C.P.; Gomes, A. L. V.; Alves, Clebert José.
É relatado o primeiro isolamento de Actinobacillus seminis em um caprino no município de Patos, Estado da Paraíba, região semiárida do Brasil. Em um rebanho com 70 caprinos e 65 ovinos criados juntos, um reprodutor da espécie caprina da raça Moxotó, com quatro anos de idade, apresentou um quadro clínico de orquite e epididimite unilateral com consistência firme. O diagnóstico da infecção por A. seminis foi confirmado pela associação dos achados clínicos, isolamento bacteriano e, PCR e sequenciamento do gene 16S do rRNA. O resultado obtido sugere que A. seminis pode ser uma importante causa de infertilidade em caprinos da região.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epididymitis; Goats; Actinobacillus seminis; Isolation.
Ano: 2015 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/25222
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The use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fields Electron. J. Biotechnol.
Nunkaew,Tomorn; Kantachote,Duangporn; Nitoda,Teruhiko; Kanzaki,Hiroshi.
The aims were to explore an appropriate isolating medium for obtaining purple nonsulfur bacteria (PNSB) for use as biofertilizers in saline paddy fields and to obtain pure cultures. We therefore chose a defined isolating medium containing 0.25% NaCl, (Glutamate-Acetate broth, GA) and a rice straw broth to compare them for numbers of PNSB obtained, time to obtain pure cultures, diversity and costs. A total of 30 water and 30 sediment samples were collected from saline paddy fields in southern Thailand and used to isolate PNSB in both the isolating media. Based on 60 samples and a period of 13 days incubation under anaerobic light conditions, a greater number of samples produced PNSB growth in GA broth after only day 3; however, after that the rice straw...
Tipo: Journal article Palavras-chave: Biofertilizer; Isolation; Paddy field; Purple nonsulfur bacteria; Rice straw broth.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600007
Registros recuperados: 19
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