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| Registros recuperados: 26 | |
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| Phonsakhan,Wipada; Kong-Ngern,Kanlaya. |
| Background Black glutinous rice contains remarkable levels of anthocyanins, which possess anti-oxidative properties and thus have health benefits. The accumulation of anthocyanins in grains of thirty black glutinous rice varieties was measured, and the results revealed that the accumulated anthocyanin content ranged from 0.262 to 2.539 mg/g. Black glutinous rice Br no. 19 was selected, and its leaf protein expression profile was compared with that of white glutinous rice RD 6 using 2D-PAGE, and the protein spots were then directly analyzed after proteolysis by LC-MS/MS. Results The proteins from the leaves of the two rice varieties were separated using 2D-PAGE and silver stained, and the spots were analyzed using Image Master 2D Platinum version 5... |
| Tipo: Journal article |
Palavras-chave: 2D-PAGE; Anthocyanin; LC-MS/MS; Oryza sativa L; Proteomics. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100006 |
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| Tolosa, Imma; Huertas, David; Choyke, Sarah; Sander, Sylvia; Aminot, Yann. |
| Two different sample preparation protocols for the determination of 37 emerging and historical halogenated flame retardants (HFRs) in marine tissues were compared with regards to extraction recovery, lipid removal efficiency, repeatability, reproducibility, and ability to measure sub-ng g−1 (dry weight) concentrations in marine biota. One method involved a purification step using gel permeation chromatography (GPC) followed by a HPLC fractionation step on a Partisil amino-cyano normal phase (GPC-Partisil procedure) and the other more traditional method was based on sulphuric acid treatment followed by silica column fractionation (H2SO4-silica procedure). The samples were analysed by gas chromatography (GC) and liquid chromatography (LC) tandem mass... |
| Tipo: Text |
Palavras-chave: Halogenated flame retardants; Biota; Clean-up; GC-MS/MS; LC-MS/MS; Emerging contaminants. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00654/76570/77700.pdf |
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| Reveillon, Damien. |
| BMAA (â-N-methylamino-L-alanine) is a non proteinogenic neurotoxic amino acid that has been associated with amyotrophic lateral sclerosis. Its analytical detection is widely debated thus the global production of BMAA by cyanobacteria is questioned. We first aimed at developing an analytical method allowing for highly selective identification of BMAA and isomers, using HILIC chromatography coupled to mass spectrometry. Then, we have cultivated and analyzed more than 50 microalgae (e.g. cyanobacteria, diatoms). Unlike DAB, one of its natural isomer, BMAA was only reported in five diatom species and cannot be accounted as a cyanotoxin anymore. In parallel, we have evaluated the presence of BMAA on the three French coasts. The presence of BMAA in different... |
| Tipo: Text |
Palavras-chave: DAB; Cyanotoxines; CL-SM/SM; Diatomées; Cyanobactéries; Bivalves; BMAA; DAB; Cyanotoxins; LC-MS/MS; Diatoms; Cyanobacteria; Bivalves. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00295/40651/39617.pdf |
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| Aasen, John A. B.; Espenes, Arild; Miles, Christopher O.; Samdal, Ingunn A.; Hess, Philipp; Aune, Tore. |
| For many years, the presence of yessotoxins (YTXs) in shellfish has contributed to the outcome of the traditional mouse bioassay and has on many occasions caused closure of shellfisheries. Since YTXs do not appear to cause diarrhoea in man and exert low oral toxicity in animal experiments, it has been suggested that they should be removed from regulation. Before doing so, it is important to determine whether the oral toxicity of YTXs is enhanced when present together with shellfish toxins known to cause damage to the gastrointestinal tract. Consequently, mice were given high doses of YTX, at 1 or 5 mg/kg body weight, either alone or together with azaspiracid-1 (AZA1) at 200 μg/kg. The latter has been shown to induce damage to the small intestine at this... |
| Tipo: Text |
Palavras-chave: Azaspiracid-1; AZA1; Yessotoxin; YTX; Marine algal toxins; Absorption; Pathology; Sublethal; NMRI; Mice; LC-MS/MS; Oral toxicity. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00034/14477/11776.pdf |
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| Bandeira,Raquel Duarte da Costa Cunha; Uekane,Thais Matsue; Cunha,Carolina Passos da; Rodrigues,Janaina Marques; la Cruz,Marcus Henrique Campino de; Godoy,Ronoel Luiz de Oliveira; Fioravante,Andreia de Lima. |
| Two analytical methods for the determination and confirmation of ochratoxin A (OTA) in green and roasted coffee samples were compared. Sample extraction and clean-up were based on liquid-liquid phase extraction and immunoaffinity column. The detection of OTA was carried out with the high performance liquid chromatography (HPLC) combined either with fluorescence detection (FLD), or positive electrospray ionization (ESI+) coupled with tandem mass spectrometry (MS-MS). The results obtained with the LC-ESI-MS/MS were specific and more sensitive, with the advantages in terms of unambiguous analyte identification, when compared with the HPLC-FLD. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HPLC-FLD; LC-MS/MS; Green Coffee and Roasted Coffee. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600004 |
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| Brissard, Charline; Herrenknecht, Christine; Sechet, Veronique; Herve, Fabienne; Pisapia, Francesco; Harcouet, Jocelyn; Lemee, Rodolphe; Chomerat, Nicolas; Hess, Philipp; Amzil, Zouher. |
| Ostreopsis cf. ovata produces palytoxin analogues including ovatoxins (OVTXs) and a putative palytoxin (p-PLTX), which can accumulate in marine organisms and may possibly lead to food intoxication. However, purified ovatoxins are not widely available and their toxicities are still unknown. The aim of this study was to improve understanding of the ecophysiology of Ostreopsis cf. ovata and its toxin production as well as to optimize the purification process for ovatoxin. During Ostreopsis blooms in 2011 and 2012 in Villefranche-sur-Mer (France, NW Mediterranean Sea), microalgae epiphytic cells and marine organisms were collected and analyzed both by LC-MS/MS and hemolysis assay. Results obtained with these two methods were comparable, suggesting ovatoxins... |
| Tipo: Text |
Palavras-chave: Ostreopsis cf. ovata; Palytoxin & ovatoxins; Culture; Seafood contamination; LC-MS/MS; Hemolysis assay; Mediterranean Sea. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00199/31025/29428.pdf |
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| Gouvêa,R.; Santos,F.F.; Machado,L.S.; Panzenhagen,P.H.N.; Aquino,M.H.C.; Nascimento,E. Rosendo do; Ribeiro,C.; Pereira,V.L.A.. |
| A enrofloxacina é um dos antimicrobianos mais utilizados na avicultura industrial, e a deposição de resíduos em produtos avícolas, como os ovos, são de grande importância para a saúde pública. Na legislação brasileira não existe padronização do período de carência para o seu uso na produção avícola e não há Limite Máximo de Resíduo (LMR) fixado para enrofloxacina em ovos. Neste estudo, foi utilizado o kit de ELISA comercial (Bioo Scientific(r)) e a LC-MS/MS na pesquisa de enrofloxacina em ovos de 30 galinhas tratadas previamente via água de bebida, com 10mg/kg de enrofloxacina, durante cinco dias. Seis ovos foram coletados diariamente e analisados durante o tratamento e após a sua suspensão, durante 15 dias. A deposição de resíduos obteve níveis máximos no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enrofloxacina; Resíduos; Ovos; LC-MS/MS; ELISA. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601917 |
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| Jauffrais, Thierry; Kilcoyne, Jane; Herrenknecht, Christine; Truquet, Philippe; Sechet, Veronique; Miles, Christopher O.; Hess, Philipp. |
| The relationship between azaspiracid shellfish poisoning and a small dinoflagellate, Azadinium spinosum, has been shown recently. The organism produces AZA1 and -2, while AZA3 and other analogues are metabolic products formed in shellfish. We evaluated whether mussels were capable of accumulating dissolved AZA1 and -2, and compared the toxin profiles of these mussels at 24 h with profiles of those exposed to live or lysed A. spinosum. We also assessed the possibility of preparative production of AZA metabolites by exposing mussels to semi-purified AZA1. We exposed mussels to similar concentration of AZAs: dissolved AZA1+2 (crude extract) at 7.5 and 0.75 µg L-1, dissolved AZA1+2 (7.5 µg L-1) in combination with Isochrysis affinis galbana, and lysed and live... |
| Tipo: Text |
Palavras-chave: Dissolved marine biotoxins; AZA; Tissue distribution; Bivalve molluscs; LC-MS/MS; Azaspiracid. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00136/24757/22867.pdf |
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| Jauffrais, Thierry; Sechet, Veronique; Truquet, Philippe; Amzil, Zouher; Herrenknecht, Christine; Hess, Philipp. |
| Azadinium spinosum, a small dinoflagellate has recently been discovered and identified as the primary producer of azaspiracid-1 (AZA) and -2. Since AZA poisoning has been reported following consumption of contaminated shellfish it is important to have these toxins available for toxicological studies, and a sustainable production of AZAs as calibrants in monitoring programs without having to rely on natural events. In order to address this concern, continuous pilot scale cultures were carried out to evaluate the feasibility of AZA production from A. spinosum. Algae were cultured using two 100 L chemostats in series (R1 and R2), with agitation and pH control. Four different dilution rates were tested (0.15, 0.2, 0.25 and 0.3 day−1) to evaluate chemostat... |
| Tipo: Text |
Palavras-chave: Solid phase adsorption; Chemostat; Photobioreactor; Microalgae; LC-MS/MS; Tangential flow filtration. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00152/26349/24437.pdf |
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| Castilhos,Juliana Kich; Dillenburg,Thaís Luise; Antunes,Marina Venzon; Scribel,Letícia; Zavascki,Alexandre Prehn; Linden,Rafael; Verza,Simone Gasparin. |
| Polymyxins are a group of antibacterial substances and have remained the drugs of choice for treatment of resistant Gram-negative bacilli. Polymyxin B is administered by intravenous infusion and requires the reconstitution of lyophilized powder with 0.9% saline or 5% glucose solutions. To date, there is little information about polymyxin stability in different infusions solutions, especially at 40 ºC, a temperature that is recommended to study drug stability as it accelerates degradation reactions. Therefore, in this work an analytical method using LC-MS/MS was developed, validated and applied to determine the stability of polymyxin B diluted in 0.9% saline or 5% glucose solutions at 25 ºC and 40 ºC. The stability of polymyxin B solutions was evaluated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polymyxin; Stability; LC-MS/MS; Glucose solutions; Saline solutions. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100537 |
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| Roue, Melanie; Darius, Helene Taiana; Picot, Sandy; Ung, Andre; Viallon, Jerome; Gaertner-mazouni, Nabila; Sibat, Manoella; Amzil, Zouher; Chinain, Mireille. |
| Ciguatera Fish Poisoning (CFP) is a foodborne disease classically related to the consumption of tropical coral reef fishes contaminated with ciguatoxins (CTXs), neurotoxins produced by dinoflagellates of the Gambierdiscus genus. Severe atypical ciguatera-like incidents involving giant clams, a marine resource highly consumed in the South Pacific, are also frequently reported in many Pacific Islands Countries and Territories. The present study was designed to assess the ability of giant clams to accumulate CTXs in their tissues and highlight the potential health risks associated with their consumption. Since giant clams are likely to be exposed to both free-swimming Gambierdiscus cells and dissolved CTXs in natural environment, ex situ contamination... |
| Tipo: Text |
Palavras-chave: Giant clams; Ex situ contamination; Ciguatoxins; Gambierdiscus polynesiensis; LC-MS/MS; Neuroblastoma cell-based assay. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00343/45415/45123.pdf |
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| Gomes,G.L.G.C.; Carbonari,C.A.; Velini,E.D.; Trindade,M.L.B.; Silva,J.R.M.. |
| This study has aimed to develop a method for simultaneous extraction and determination by liquid chromatography and mass spectrometry (LC-MS/MS) of glyphosate, aminomethylphosphonic acid (AMPA), shikimic acid, quinic acid, phenylalanine, tyrosine and tryptophan. For the joint analysis of these compounds the best conditions of ionization in mass spectrometry and for chromatographic separation of the compounds were selected. Calibration curves and linearity ranges were also determined for each compound. Different extraction systems of the compounds were tested from plant tissues collected from sugarcane (Saccharum officinarum) and eucalyptus (Eucalyptus urophylla platiphylla) plants two days after the glyphosate application at the dose of 720 g a.e. ha-1.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Herbicide; LC-MS/MS; Metabolites; Metabolic profile. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582015000200295 |
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| Pais,Mariana Castello Novo; Nascimento,Elizabeth de Souza. |
| Abstract This study aimed to determine whether the anti-inflammatory drugs that are most commonly consumed in Brazil, including diclofenac, ketoprofen, naproxen, indomethacin, ibuprofen and acetaminophen, are present in drinking water and to derive guideline values to characterize the human risk. These pharmaceuticals were quantified in surface waters by LC-MS/MS with solid phase extraction, both before and after conventional treatment on a laboratory scale, using a jar test assay. The methods used to quantify these drugs showed good results: the chromatographic analysis obtained correlation coefficients between 0.9952 and 0.9991, with limits of quantification of 0.5 ng.mL-1 - 50 ng.mL-1 and precision standard deviations (0.08 - 2.08). Only ketoprofen and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Emerging contaminant; LC-MS/MS; Human risk assessment; Pharmaceuticals in drinking water.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100624 |
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| SANTOS,E.A.; CORREIA,N.M.; SILVA,J.R.M.; VELINI,E.D.; PASSOS,A.B.R.J.; DURIGAN,J.C.. |
| Due to the large amount of pesticides applied in agriculture, mainly herbicides, there is a growing concern about a possible environmental contamination with these products, including water bodies. Given the above, the aim of the present work was to detect and quantify herbicides through multiresidue analysis in water samples collected in semi-artesian wells and springs in a rural area of the city of Jaboticabal (SP). Samples were collected from 32 wells and 13 water springs, in three different seasons: October 2010, February 2011 and May 2011. Additionally, samples at a residence in the urban area were also collected. Analysis using high performance liquid chromatography coupled to mass spectrometry was performed and herbicides ametryn, amicarbazone,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sugarcane; LC-MS/MS; Water springs; Semi-artesian wells. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582015000100147 |
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| Longo, Sebastien; Sibat, Manoella; Viallon, Jérôme; Darius, Hélène Taiana; Hess, Philipp; Chinain, Mireille. |
| Ciguatera poisoning (CP) is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genera Gambierdiscus and Fukuyoa. The toxin production and toxin profiles were explored in four clones of G. polynesiensis originating from different islands in French Polynesia with contrasted CP risk: RIK7 (Mangareva, Gambier), NHA4 (Nuku Hiva, Marquesas), RAI-1 (Raivavae, Australes), and RG92 (Rangiroa, Tuamotu). Productions of CTXs, maitotoxins (MTXs), and gambierone group analogs were examined at exponential and stationary growth phases using the neuroblastoma cell-based assay and liquid chromatography–tandem mass spectrometry. While none of the strains was found to produce known MTX compounds,... |
| Tipo: Text |
Palavras-chave: Gambierdiscus polynesiensis; Ciguatera; Ciguatoxins; Gambierone; 44-methylgambierone; LC-MS/MS; CBA-N2a; Toxin profiles. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00599/71134/69449.pdf |
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| Ben Haddouch, Asia; Amanhi, Rachid; Amzil, Zouher; Taleb, Hamib; Rovillon, Georges-augustin; Adly, Farida; Loutfi, Mohammed. |
| Forthe Moroccan Phycotoxins Monitoring that is part of theSafety of theCoastal Monitoring Network(RSSL),shellfish sampleswereharvestedfromdifferentlocations at NorthAtlantic of Morocco whereharmful algaeblooms were known to have occurred. Forallshellfishsamples found positive by themouse bioassay fordiarrheicshellfishpoisoning(DSP)toxins,liquid chromatography coupled to tandem massspectrometry(LC-MS-MS) in order to search thefollowinglipophilictoxins: okadaic acid (OA), dinophysistoxins(DTXs), pectenotoxins(PTXs), azaspiracids(AZAs), yessotoxins(YTXs), spirolides(SPXs) and gymnodimines (GYMs).Theresultsrevealeddifferentlipophilictoxinprofiles as a function of theshellfish sampling location. It has beennoticed that allthesamples contained OA and its... |
| Tipo: Text |
Palavras-chave: Diarrheictoxins; Lipophilic toxins; LC-MS/MS; Mouse test; Shellfish. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00372/48342/48495.pdf |
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| Ferreira,Adson da S. G.; Silva,Henrique C. M. P. da; Rodrigues,Hélio O. S.; Silva,Marileide; Albuquerque Junior,Eden C. de. |
| ABSTRACT The intensive use of pesticides to control pests in agriculture has exposed the environment and humans to a variety of risks. Among the crops with higher consumption of these compounds there is the sugarcane, developed in regions bordered by large watersheds. In this work, the occurrence of pesticides in the water of Ipojuca River was investigated in a 50 km range of its eastern portion, in a region noted for intense agroindustrial activity, especially by sugarcane cultivation, in the state of Pernambuco. Among fungicides, herbicides, insecticides and carbamates, 238 pesticides were investigated in the Ipojuca River using the technique of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The study, conducted in the months of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sugarcane; Pesticides; Environmental contamination; LC-MS/MS. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662016001201124 |
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| Amzil, Zouher; Sibat, Manoella; Chomerat, Nicolas; Grossel, Hubert; Marco-miralles, Francoise; Lemee, Rodolphe; Nezan, Elisabeth; Sechet, Veronique. |
| Dinoflagellates of the genus Ostreopsis are known to cause (often fatal) food poisoning in tropical coastal areas following the accumulation of palytoxin (PLTX) and/or its analogues (PLTX group) in crabs, sea urchins or fish. Ostreopsis spp. occurrence is presently increasing in the northern to north western Mediterranean Sea (Italy, Spain, Greece and France), probably in response to climate change. In France, Ostreopsis. cf. ovata has been associated with toxic events during summer 2006, at Morgiret, off the coast of Marseille, and a specific monitoring has been designed and implemented since 2007. Results from 2008 and 2009 showed that there is a real danger of human poisoning, as these demonstrated bioaccumulation of the PLTX group (PLTX and ovatoxin-a)... |
| Tipo: Text |
Palavras-chave: Ostreopsis cf. ovata; Palytoxin; Ovatoxin-a; LC-MS/MS; Seafood. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00071/18230/15799.pdf |
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| Registros recuperados: 26 | |
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