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Registros recuperados: 21
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A comparative proteomic study of white and black glutinous rice leaves Electron. J. Biotechnol.
Phonsakhan,Wipada; Kong-Ngern,Kanlaya.
Background Black glutinous rice contains remarkable levels of anthocyanins, which possess anti-oxidative properties and thus have health benefits. The accumulation of anthocyanins in grains of thirty black glutinous rice varieties was measured, and the results revealed that the accumulated anthocyanin content ranged from 0.262 to 2.539 mg/g. Black glutinous rice Br no. 19 was selected, and its leaf protein expression profile was compared with that of white glutinous rice RD 6 using 2D-PAGE, and the protein spots were then directly analyzed after proteolysis by LC-MS/MS. Results The proteins from the leaves of the two rice varieties were separated using 2D-PAGE and silver stained, and the spots were analyzed using Image Master 2D Platinum version 5...
Tipo: Journal article Palavras-chave: 2D-PAGE; Anthocyanin; LC-MS/MS; Oryza sativa L; Proteomics.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100006
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BMAA et cyanotoxines : microalgues productrices et niveaux d’accumulation dans les organismes marins ArchiMer
Reveillon, Damien.
BMAA (â-N-methylamino-L-alanine) is a non proteinogenic neurotoxic amino acid that has been associated with amyotrophic lateral sclerosis. Its analytical detection is widely debated thus the global production of BMAA by cyanobacteria is questioned. We first aimed at developing an analytical method allowing for highly selective identification of BMAA and isomers, using HILIC chromatography coupled to mass spectrometry. Then, we have cultivated and analyzed more than 50 microalgae (e.g. cyanobacteria, diatoms). Unlike DAB, one of its natural isomer, BMAA was only reported in five diatom species and cannot be accounted as a cyanotoxin anymore. In parallel, we have evaluated the presence of BMAA on the three French coasts. The presence of BMAA in different...
Tipo: Text Palavras-chave: DAB; Cyanotoxines; CL-SM/SM; Diatomées; Cyanobactéries; Bivalves; BMAA; DAB; Cyanotoxins; LC-MS/MS; Diatoms; Cyanobacteria; Bivalves.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00295/40651/39617.pdf
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Combined oral toxicity of azaspiracid-1 and yessotoxin in female NMRI mice ArchiMer
Aasen, John A. B.; Espenes, Arild; Miles, Christopher O.; Samdal, Ingunn A.; Hess, Philipp; Aune, Tore.
For many years, the presence of yessotoxins (YTXs) in shellfish has contributed to the outcome of the traditional mouse bioassay and has on many occasions caused closure of shellfisheries. Since YTXs do not appear to cause diarrhoea in man and exert low oral toxicity in animal experiments, it has been suggested that they should be removed from regulation. Before doing so, it is important to determine whether the oral toxicity of YTXs is enhanced when present together with shellfish toxins known to cause damage to the gastrointestinal tract. Consequently, mice were given high doses of YTX, at 1 or 5 mg/kg body weight, either alone or together with azaspiracid-1 (AZA1) at 200 μg/kg. The latter has been shown to induce damage to the small intestine at this...
Tipo: Text Palavras-chave: Azaspiracid-1; AZA1; Yessotoxin; YTX; Marine algal toxins; Absorption; Pathology; Sublethal; NMRI; Mice; LC-MS/MS; Oral toxicity.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00034/14477/11776.pdf
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Complex Toxin Profile of French Mediterranean Ostreopsis cf. ovata Strains, Seafood Accumulation and Ovatoxins Prepurification ArchiMer
Brissard, Charline; Herrenknecht, Christine; Sechet, Veronique; Herve, Fabienne; Pisapia, Francesco; Harcouet, Jocelyn; Lemee, Rodolphe; Chomerat, Nicolas; Hess, Philipp; Amzil, Zouher.
Ostreopsis cf. ovata produces palytoxin analogues including ovatoxins (OVTXs) and a putative palytoxin (p-PLTX), which can accumulate in marine organisms and may possibly lead to food intoxication. However, purified ovatoxins are not widely available and their toxicities are still unknown. The aim of this study was to improve understanding of the ecophysiology of Ostreopsis cf. ovata and its toxin production as well as to optimize the purification process for ovatoxin. During Ostreopsis blooms in 2011 and 2012 in Villefranche-sur-Mer (France, NW Mediterranean Sea), microalgae epiphytic cells and marine organisms were collected and analyzed both by LC-MS/MS and hemolysis assay. Results obtained with these two methods were comparable, suggesting ovatoxins...
Tipo: Text Palavras-chave: Ostreopsis cf. ovata; Palytoxin & ovatoxins; Culture; Seafood contamination; LC-MS/MS; Hemolysis assay; Mediterranean Sea.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00199/31025/29428.pdf
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Desenvolvimento e validação de método para determinação de florfenicol e seu metabólito em tilápia por LC-MS/MS. Repositório Alice
SHIROMA, L. S.; JONSSON, C. M.; QUEIROZ, S. C. do N. de; BOTTOLI, C. B. G..
Atualmente há um crescimento na produção de pescado no mundo devido à busca dos consumidores por alimentos mais saudáveis e com alto valor nutritivo.1 Para atender a esse aumento na demanda de pescados surgiu o cultivo intensivo. Porém neste sistema de produção há uma maior ocorrência de infecções, pois a alta densidade populacional de peixes resulta em má qualidade da água, mudanças nos valores de pH, oxigênio, maior acúmulo de resíduos orgânicos e nutrição insuficiente dos peixes. Essas infecções podem ocasionar lesões superficiais, nas barbatanas, nas caudas e doenças branquiais.2 Para controlar isso, são administrados antibióticos, que são compostos que previnem ou tratam infecções microbianas. Entretanto o uso indiscriminado dessas substâncias pode...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: LC-MS/MS; Tilápia; Florfenicol.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103261
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Determinação de resíduos de paclobutrazol em manga (Mangifera indida L.) por cromatografia acoplada a espectometria de massas (LC-MS/MS). Infoteca-e
FERRACINI, V. L.; QUEIROZ, S. C. do N. de; ROSA, M. A.; LOPES, P. R. C..
Paclobutrazol (PBZ) é um regulador de crescimento que tem sido utilizado para alterar o ciclo fenológico da mangueira visando a produção de frutos em qualquer época do ano. É bastante utilizado na região nordeste do Brasil e a determinação dos resíduos na fruta é importante pois há pouco conhecimen- to sobre o acúmulo desta substância na planta em condições brasileiras. Para isso foi necessário adaptar um método analítico para determinar resídu- os de paclobutrazol em frutos de manga. Após a extração do PBZ na matriz o extrato foi analisado por LC-MS/MS. Os limites de detecção (LOD) e de quantificação (LOQ) obtidos para o PBZ foram de 0,25 ?g Kg-1 e 1,25 ?g Kg-1, respectivamente. As recuperações foram de 82 a 94% e se encontram dentro da faixa considerada...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: LC-MS/MS; Resíduos de paclobutrazol; Resíduo químico; Regulador de crescimento; Análise química; Especrometria; Cromatografia; Manga; Paclobutrazol; Chemical residues; Chemical analysis; Chromatography; Mass spectrometry.
Ano: 2011 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/898423
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Determination of moxidectin in serum by liquid chromatography-tandem mass spectrometry and its application in pharmacokinetic study in lambs. Repositório Alice
BAPTISTA, R. C.; FERNANDES, M. A. M.; GILAVERTE, S.; QUEIROZ, S. C. do N. de; ASSALIN, M. R.; FERRACINI, V. L.; MONTEIRO, A. L. G.; REYES, F. G. R..
Abstract: The widespread use of moxidectin (MOX), a parasiticide used in the sheep breeding, has induced the parasite resistance in Brazilian farms. As a consequence, the farmers often increase the dose and frequency of drug utilization, and disregards safety of meat or milk. In order to establish adequate therapeutic treatment it is necessary to know the pharmacokinetics of the drug in the animal's body. Thus, high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed for the determination of MOX in serum lamb. Serum samples were treated with acetonitrile to precipitate proteins. A clean up by dispersive extraction in solid phase (SPE-d), using primary/secondary amine (PSA) and C18...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Serum lamb; LC-MS/MS; Cordeiro; Soro sanguíneo; Análise de laboratório; Anti-helmíntico; Cromatografia; Espectrometria; Blood serum; Lambs; Moxidectin; Anthelmintics; Pharmacokinetics; Veterinary drugs; Tandem mass spectrometry; Liquid chromatography.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1060930
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Dissolved azaspiracids are absorbed and metabolized by blue mussels (Mytilus edulis) ArchiMer
Jauffrais, Thierry; Kilcoyne, Jane; Herrenknecht, Christine; Truquet, Philippe; Sechet, Veronique; Miles, Christopher O.; Hess, Philipp.
The relationship between azaspiracid shellfish poisoning and a small dinoflagellate, Azadinium spinosum, has been shown recently. The organism produces AZA1 and -2, while AZA3 and other analogues are metabolic products formed in shellfish. We evaluated whether mussels were capable of accumulating dissolved AZA1 and -2, and compared the toxin profiles of these mussels at 24 h with profiles of those exposed to live or lysed A. spinosum. We also assessed the possibility of preparative production of AZA metabolites by exposing mussels to semi-purified AZA1. We exposed mussels to similar concentration of AZAs: dissolved AZA1+2 (crude extract) at 7.5 and 0.75 µg L-1, dissolved AZA1+2 (7.5 µg L-1) in combination with Isochrysis affinis galbana, and lysed and live...
Tipo: Text Palavras-chave: Dissolved marine biotoxins; AZA; Tissue distribution; Bivalve molluscs; LC-MS/MS; Azaspiracid.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00136/24757/22867.pdf
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Écophysiologie des dinoflagellés du genre Azadinium, production toxinique et transfert trophique vers les mollusques bivalves ArchiMer
Jauffrais, Thierry.
This study has been conducted in order to develop the analysis of AZAs and to produce AZAs from A. spinosum culture. It also aimed at studying the effect of environmental and nutritional factors on growth and toxin production. The study also demonstrated a link between A. spinosum and the accumulation of AZAs in shellfish, followed by the clarification of the processes of accumulation, detoxification and biotransformation of AZAs into mussels. A quantitative analysis of AZAs in A. spinosum cultures was developed, and the formation and structure of the AZA methylated analogues was explained and minimised. This work also demonstrated the feasibility of a sustainable production of AZAs from A. spinosum culture and highlighted the main factors influencing...
Tipo: Text Palavras-chave: Azadinium spinosum; Mytilus edulis; Azaspiracides; Phycotoxines; Dinoflagellés; Écophysiologie; CL-SM/SM; Azadinium spinosum; Mytilus edulis; Azaspiracids; Phycotoxins; Dinoflagellate; Ecophysiology; LC-MS/MS.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00111/22217/19891.pdf
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Effect of Dilution Rate on Azadinium spinosum and Azaspiracid (AZA) Production in Pilot Scale Photobioreactors for the Harvest of AZA1 and -2 ArchiMer
Jauffrais, Thierry; Sechet, Veronique; Truquet, Philippe; Amzil, Zouher; Herrenknecht, Christine; Hess, Philipp.
Azadinium spinosum, a small dinoflagellate has recently been discovered and identified as the primary producer of azaspiracid-1 (AZA) and -2. Since AZA poisoning has been reported following consumption of contaminated shellfish it is important to have these toxins available for toxicological studies, and a sustainable production of AZAs as calibrants in monitoring programs without having to rely on natural events. In order to address this concern, continuous pilot scale cultures were carried out to evaluate the feasibility of AZA production from A. spinosum. Algae were cultured using two 100 L chemostats in series (R1 and R2), with agitation and pH control. Four different dilution rates were tested (0.15, 0.2, 0.25 and 0.3 day−1) to evaluate chemostat...
Tipo: Text Palavras-chave: Solid phase adsorption; Chemostat; Photobioreactor; Microalgae; LC-MS/MS; Tangential flow filtration.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00152/26349/24437.pdf
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Evidence of the bioaccumulation of ciguatoxins in giant clams (Tridacna maxima) exposed to Gambierdiscus spp. cells ArchiMer
Roue, Melanie; Darius, Helene Taiana; Picot, Sandy; Ung, Andre; Viallon, Jerome; Gaertner-mazouni, Nabila; Sibat, Manoella; Amzil, Zouher; Chinain, Mireille.
Ciguatera Fish Poisoning (CFP) is a foodborne disease classically related to the consumption of tropical coral reef fishes contaminated with ciguatoxins (CTXs), neurotoxins produced by dinoflagellates of the Gambierdiscus genus. Severe atypical ciguatera-like incidents involving giant clams, a marine resource highly consumed in the South Pacific, are also frequently reported in many Pacific Islands Countries and Territories. The present study was designed to assess the ability of giant clams to accumulate CTXs in their tissues and highlight the potential health risks associated with their consumption. Since giant clams are likely to be exposed to both free-swimming Gambierdiscus cells and dissolved CTXs in natural environment, ex situ contamination...
Tipo: Text Palavras-chave: Giant clams; Ex situ contamination; Ciguatoxins; Gambierdiscus polynesiensis; LC-MS/MS; Neuroblastoma cell-based assay.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00343/45415/45123.pdf
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Identificação de proteínas envolvidas na aquisição de competência embriogênica em dendezeiros (Elaeis guineensis Jacq. var. tenera) utilizando proteômica Shotgun. Repositório Alice
RIBEIRO, D. G.; ALMEIDA, R. F.; FONTES, W.; CASTRO, M. de S.; SOUSA, M. V. de; RICART, C. A. O.; PEREIRA, J. E. S.; MEHTA, A..
2016
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Embriogênese somática; LC-MS/MS; Proteínas diferenciais.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1066037
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Lipophilic Toxin Profile in Mytilus galloprovincialis from the North Atlantic Coast of Morocco: LC-MS/MS and Mouse Bioassay Analyses ArchiMer
Ben Haddouch, Asia; Amanhi, Rachid; Amzil, Zouher; Taleb, Hamib; Rovillon, Georges-augustin; Adly, Farida; Loutfi, Mohammed.
Forthe Moroccan Phycotoxins Monitoring that is part of theSafety of theCoastal Monitoring Network(RSSL),shellfish sampleswereharvestedfromdifferentlocations at NorthAtlantic of Morocco whereharmful algaeblooms were known to have occurred. Forallshellfishsamples found positive by themouse bioassay fordiarrheicshellfishpoisoning(DSP)toxins,liquid chromatography coupled to tandem massspectrometry(LC-MS-MS) in order to search thefollowinglipophilictoxins: okadaic acid (OA), dinophysistoxins(DTXs), pectenotoxins(PTXs), azaspiracids(AZAs), yessotoxins(YTXs), spirolides(SPXs) and gymnodimines (GYMs).Theresultsrevealeddifferentlipophilictoxinprofiles as a function of theshellfish sampling location. It has beennoticed that allthesamples contained OA and its...
Tipo: Text Palavras-chave: Diarrheictoxins; Lipophilic toxins; LC-MS/MS; Mouse test; Shellfish.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00372/48342/48495.pdf
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Ovatoxin-a and Palytoxin Accumulation in Seafood in Relation to Ostreopsis cf. ovata Blooms on the French Mediterranean Coast ArchiMer
Amzil, Zouher; Sibat, Manoella; Chomerat, Nicolas; Grossel, Hubert; Marco-miralles, Francoise; Lemee, Rodolphe; Nezan, Elisabeth; Sechet, Veronique.
Dinoflagellates of the genus Ostreopsis are known to cause (often fatal) food poisoning in tropical coastal areas following the accumulation of palytoxin (PLTX) and/or its analogues (PLTX group) in crabs, sea urchins or fish. Ostreopsis spp. occurrence is presently increasing in the northern to north western Mediterranean Sea (Italy, Spain, Greece and France), probably in response to climate change. In France, Ostreopsis. cf. ovata has been associated with toxic events during summer 2006, at Morgiret, off the coast of Marseille, and a specific monitoring has been designed and implemented since 2007. Results from 2008 and 2009 showed that there is a real danger of human poisoning, as these demonstrated bioaccumulation of the PLTX group (PLTX and ovatoxin-a)...
Tipo: Text Palavras-chave: Ostreopsis cf. ovata; Palytoxin; Ovatoxin-a; LC-MS/MS; Seafood.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00071/18230/15799.pdf
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Production and Isolation of Azaspiracid-1 and -2 from Azadinium spinosum Culture in Pilot Scale Photobioreactors ArchiMer
Jauffrais, Thierry; Kilcoyne, Jane; Sechet, Veronique; Herrenknecht, Christine; Truquet, Philippe; Herve, Fabienne; Berard, Jean-baptiste; Nulty, Ciara; Taylor, Sarah; Tillmann, Urban; Miles, Christopher O.; Hess, Philipp.
Azaspiracid (AZA) poisoning has been reported following consumption of contaminated shellfish, and is of human health concern. Hence, it is important to have sustainable amounts of the causative toxins available for toxicological studies and for instrument calibration in monitoring programs, without having to rely on natural toxin events. Continuous pilot scale culturing was carried out to evaluate the feasibility of AZA production using Azadinium spinosum cultures. Algae were harvested using tangential flow filtration or continuous centrifugation. AZAs were extracted using solid phase extraction (SPE) procedures, and subsequently purified. When coupling two stirred photobioreactors in series, cell concentrations reached 190,000 and 210,000 cell·mL−1 at...
Tipo: Text Palavras-chave: Solid phase extraction; Photobioreactor; Chemostat; Dinoflagellate; Micro-algae; LC-MS/MS; Tangential flow filtration; Azaspiracid; HP-20.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00088/19875/17526.pdf
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Proteomic analysis and functional validation of a Brassica oleracea Endochitinase involved in resistance to Xanthomonas campestris. Repositório Alice
SANTOS, C.; NOGUEIRA, F. C. S.; DOMONT, G. B.; FONTES, W.; PRADO, G. S.; HABIBI, P.; SANTOS, V. O.; OLIVEIRA-NETO, O. B.; SA, M. F. G. de; JORRÍN-NOVO, J. V.; FRANCO, O. L.; REIS, A. M. dos.
bitstream/item/199229/1/Santos-et-al-2019-Frontiers-in-Plant-Science.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: LC-MS/MS; Differential protein abundance; QRT-PCR; Plant?pathogen interaction; Gene overexpression.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1110405
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Quantitative analysis of azaspiracids in Azadinium spinosum cultures ArchiMer
Jauffrais, Thierry; Herrenknecht, Christine; Sechet, Veronique; Sibat, Manoella; Tillmann, Urban; Krock, Bernd; Kilcoyne, Jane; Miles, Christopher O.; Mccarron, Pearse; Amzil, Zouher; Hess, Philipp.
Azaspiracids (AZAs) are secondary metabolites of Azadinium spinosum, that have been shown to cause diarrhetic shellfish poisoning when accumulated in bivalve molluscs. We describe here an analytical procedure for the determination of AZAs in cultures of A. spinosum with a focus on the potential formation of AZA methyl-esters as artefacts in the extraction and sample pre-treatment. A. spinosum cells were collected from bioreactor cultures, using centrifugation or filtration. Different extraction procedures were evaluated for formation of methyl-ester artefacts, yield, and matrix effects. Filtration of cultures using glass-fibre filters led to increased formation of methyl-esters. Hence centrifugation is recommended for recovery of cells. The type of...
Tipo: Text Palavras-chave: Extraction procedure; Extraction artefact; Matrix effects; LC-MS/MS; Azaspiracid methyl ester; Dinoflagellate; Liquid chromatography-mass spectrometry.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00077/18812/16463.pdf
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Selectivity of pre-emergent herbicides in sweet potato genotypes. Repositório Alice
SANTOS, E. A. dos; ANDRADE JÚNIOR, V. C. de; SOUZA JÚNIOR, A. S. de; OKUMURA, F.; SIMEONE, M. L. F.; SANTOS, J. B. dos; AZEVEDO, A. M..
Sweet potato is sensitive to weeds and a chemical control is needed for high yield production. This work describes the evaluation of three sweet potato genotypes grown in pre-treated soil with clomazone, linuron, metribuzin mechanical weed control (MWC) and without weed control. Visual injury and yield of genotypes were examined, as well as production of branches and roots in fresh and dry biomass. Herbicide residues at root samples were determined by the LC-MS/MS method. The genotypes exhibited a different behaviour under clomazone, metribuzin and linuron applications. Metribuzin provided a yield decrease in all plants. Pre-treated soil with clomazone and mechanical control provided plants more productive. Linuron residues showed values of up to 0.327 ng...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Minor crops; LC-MS/MS; Controle Químico; Batata Doce; Ipomoea Batatas; Chemical control.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1095431
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The role of Azadinium spinosum (Dinophyceae) in the production of azaspiracid shellfish poisoning in mussels ArchiMer
Salas, Rafael; Tillmann, Urban; John, Uwe; Kilcoyne, Jane; Burson, Amanda; Cantwell, Caoimhe; Hess, Philipp; Jauffrais, Thierry; Silke, Joe.
Azaspiracids (AZAs) are a group of lipophilic polyether compounds first detected in Ireland which have been implicated in shellfish poisoning incidents around Europe. These toxins regularly effect shellfish mariculture operations including protracted closures of shellfish harvesting areas for human consumption. The armoured dinoflagellate Azadinium spinosum Elbrachter et Tillmann gen. et sp. nov. (Dinophyceae) has been described as the de novo azaspiracid toxin producer; nonetheless the link between this organism and AZA toxin accumulation in shellfish has not yet been established. In August 2009, shellfish samples of blue mussel (Mytilus edulis) from the Southwest of Ireland were analysed using liquid chromatography-tandem-mass spectrometry (LC-MS/MS) and...
Tipo: Text Palavras-chave: Azaspiracids; AZP; AZA toxins; Biodeposits; Dinoflagellates; Feeding experiment; Ireland; LC-MS/MS; Mussels; Phylogeny; Taxonomy.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00049/16049/15836.pdf
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Tissue Distribution and Elimination of Ciguatoxins in Tridacna maxima (Tridacnidae, Bivalvia) Fed Gambierdiscus polynesiensis ArchiMer
Roue, Melanie; Darius, Helene Taiana; Ung, Andre; Viallon, Jerome; Sibat, Manoella; Hess, Philipp; Amzil, Zouher; Chinain, Mireille.
Ciguatera is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs). Ciguatera-like poisoning events involving giant clams (Tridacna maxima) are reported occasionally from Pacific islands communities. The present study aimed at providing insights into CTXs tissue distribution and detoxification rate in giant clams exposed to toxic cells of Gambierdiscus polynesiensis, in the framework of seafood safety assessment. In a first experiment, three groups of tissue (viscera, flesh and mantle) were dissected from exposed individuals, and analyzed for their toxicity using the neuroblastoma cell-based assay (CBA-N2a) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses. The viscera, flesh, and mantle were...
Tipo: Text Palavras-chave: Giant clams; Ex situ exposure to toxic algae; Ciguatoxins; Gambierdiscus polynesiensis; Anatomical distribution; Toxin elimination; CBA-N2a; LC-MS/MS.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00440/55123/56579.pdf
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