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Registros recuperados: 33
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Biodegradation of remazol brilliant blue R by ligninolytic enzymatic complex produced by Pleurotus ostreatus BJM
Machado,Kátia Maria Gomes; Matheus,Dácio Roberto.
Pleurotus ostreatus ("shimeji") is produced in Brazil on a commercial scale using various lignocellulosic residues. Efforts have been made to reuse the culture residue to obtain products of greater aggregate value such as enzymes or in processes of bioremediation. We evaluated the Remazol brilliant blue R (RBBR) degradation potential of extracts from solid substrate colonized by P. ostreatus and extracts from residue of the "shimeji" mushroom yield. Colonized substrates and residue were provided by Toyobo do Brasil Ltda. Extraction was performed with sodium acetate buffer (50 mM, pH 4.6). RBBR decolorization was monitored at 592 nm and peroxidase and laccase activities were measured by monitoring the oxidation of ABTS. Horseradish peroxidase was used as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peroxidases; Laccase; Solid-state fermentation; "shimeji" culture residue.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400013
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Cloning, characterization and expression of a novel laccase gene Pclac2 from Phytophthora capsici BJM
Feng,Bao Zhen; Li,Peiqian.
Laccases are blue copper oxidases (E.C. 1.10.3.2) that catalyze the one-electron oxidation of phenolics, aromatic amines, and other electron-rich substrates with the concomitant reduction of O2 to H2O. A novel laccase gene pclac2 and its corresponding full-length cDNA were cloned and characterized from Phytophthora capsici for the first time. The 1683 bp full-length cDNA of pclac2 encoded a mature laccase protein containing 560 amino acids preceded by a signal peptide of 23 amino acids. The deduced protein sequence of PCLAC2 showed high similarity with other known fungal laccases and contained four copper-binding conserved domains of typical laccase protein. In order to achieve a high level secretion and full activity expression of PCLAC2, expression...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phytophthora capsici; Laccase; Expression; Purification; Activity.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100050
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Copper improves the production of laccase by the white-rot fungus Pleurotus pulmonarius in solid state fermentation BABT
Tychanowicz,Giovana Kirst; Souza,Daniela F. de; Souza,Cristina G. M.; Kadowaki,Marina Kimiko; Peralta,Rosane Marina.
Pleurotus pulmonarius (Fr) Quélet, produced laccase as the main ligninolytic enzyme when cultivated on solid-state cultures using corn cob as substrate. The addition of copper greatly increased the production of enzyme. The addition of 25.0 mM CuSO4 increased the level of laccase from 270 to 1,420 U.L-1 and the fungus showed high resistance to copper under the conditions used in this work.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Copper; Laccase; Pleurotus pulmonarius; Phenoloxidase; Solid state fermentation.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000600002
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Copper induction and differential expression of laccase in Aspergillus flavus BJM
Gomaa,Ola M.; Momtaz,Osama A..
Aspergillus flavus was isolated from soil and exhibited laccase activity under both constitutive and copper induced conditions. Spiking the medium with 1 mM copper sulfate resulted in an increase in the activity which reached 51.84 U/mL, a distinctive protein band was detected at 60 kDa. The extracellular enzyme was purified 81 fold using gel filtration chromatography and resulted in two different laccase fractions L1 and L2, the latter had a higher enzymatic activity which reached 79.57 U/mL and specific activity of 64.17 U/μg protein. The analysis of the spectrum of the L2 fraction showed a shoulder at 330 nm which is characteristic for T2/T3 copper centers; both copper and zinc were detected suggesting that this is an unconventional white laccase....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus flavus; Laccase; Copper induction; Textile waste water decolorization.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100285
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Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat ArchiMer
Luna-acosta, Andrea; Renault, Tristan; Thomas-guyon, Helene; Faury, Nicole; Saulnier, Denis; Budzinski, Helene; Le Menach, K.; Pardon, P.; Fruitier-arnaudin, I.; Bustamante, Paco.
In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially hostile environment, i.e. to an herbicide alone or within a mixture. Following 2 h of exposure to the herbicide diuron at 1 mu g L-1 the repression of different genes implicated in immune defence mechanisms in the haemocytes and the inhibition of enzyme activities, such as laccase-type phenoloxidase (PO) in the plasma, were observed. The inhibition of superoxide dismutase (SOD) activity in the plasma was also observed after 6 and 24 h of exposure. In the mixture with the herbicides...
Tipo: Text Palavras-chave: Bivalve; Pollution; Gene expression; Laccase; Superoxide dismutase; Phagocytosis.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00083/19435/17475.pdf
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EFFECT OF PENTACHLOROPHENOL ON SELECTED SOIL ENZYME ACTIVITIES IN A CHILEAN ANDISOL R.C. Suelo Nutr. Veg.
Diez J,M. Cristina; Gallardo A,Felipe; Saavedra,Gisselle; Cea L,Mara; Gianfreda,Liliana; Alvear Z,Marisol.
The activities of laccase, Mn-peroxidase, phosphatase and dehydrogenase were studied in a Chilean Andisol (Temuco Serie) contaminated with pentachlorophenol (PCP). Experiments were conducted on the top of 0-20 cm soil, adding different concentrations of PCP (0.41, 4.1 and 41 mg kg-1). Enzymatic activities and residual concentrations of PCP were evaluated in soil incubated at 25 ± 1 ºC for 112 days. Enzyme activities of PCP-treated soil varied according to the PCP concentration added. For instance, an inhibitory effect on Mn-peroxidase activity was not observed during incubation at the three levels of PCP, whereas acid phosphatase activity was only affected by the 0.41 mg kg-1 PCP treatment. By contrast, laccase and dehydrogenase activities were negatively...
Tipo: Journal article Palavras-chave: Pentachlorophenol; Andisol; Laccase; Mn-peroxidase; Phosphatase; Dehydrogenase.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912006000300004
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Enhancement of growth of Lentinus crinitus in soil using benomyl and vegetable oil BJM
Machado,Kátia M.G.; Matheus,Dácio R.; Rodrigues,Tatiana A.; Bononi,Vera L.R..
In order to identify alternatives for soil fumigation in bioremediation process, the addition of benomyl and vegetable oil on the growth of L. crinitus and mitosporic fungi were evaluated. Benomyl can be used as an alternative to methyl bromide. Addition of vegetable oil favors the growth of L. crinitus.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Benomyl; Basidiomycetes; Fungicide; Soil bioremediation; Laccase.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400004
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Growth and extracellular laccase production in liquid cultures of Minimidochium parvum LPSC # 548 Strain Boletín de la Sociedad
Saparrat,Mario C. N.; Arambarri,Angélica M.; Balatti,Pedro A..
Minimidochium parvum LPSC # 548, a fungus isolated from litter floating on waters of Río Santiago (Provincia de Buenos Aires, Argentina) polluted with industrial effluents and crude-oil, was grown as a shaking culture on a C-limited medium to evaluate its ability to produce extracellular laccase. The effect of anthracene, CuSO4 · 5H2O, ethanol, guaiacol, humic acids, Kraft lignin, MnSO4· H2O, Tween 20 and veratryl alcohol on its growth and extracellular laccase activity levels was also analyzed. The cultures grown on basal medium produced maximum biomass (over 420 mg/100 ml) and maximum extracellular laccase activity (351.7 ±53.3 pkat/ml) after 5 days of incubation. Among the different factors tested, only the humic acids at 0.1 % (w/v) were found to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Growth; Laccase; Minimidochium parvum.
Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722007000100006
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Industrial and biotechnological applications of ligninolytic enzymes of the basidiomycota: A review Electron. J. Biotechnol.
Maciel,Márcia Jaqueline Mendonça; Silva,Ademir Castro e; Ribeiro,Helena Camarão Telles.
Ligninolytic enzymes of the basidiomycetes play a crucial role in the global carbon cycle. The demand for application of ligninolytic enzymes complexes of white-rot fungi in industry and biotechnology is ever increasing due to their use in a variety of processes. Ligninolytic enzymes have potential applications in a large number of fields, including the chemical, fuel, food, agricultural, paper, textile, cosmetic industrial sectors and more. This ligninolytic system of white-rot fungi is also directly involved in the degradation of various xenobiotic compounds and dyes. Their capacities to remove xenobiotic substances and produce polymeric products make them a useful tool for bioremediation purposes. This paper reviews the applications of ligninolytic...
Tipo: Journal article Palavras-chave: Laccase; Lignin peroxidase; Manganese peroxidase; White-rot fungi.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600014
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Influence of pH on the growth, laccase activity and RBBR decolorization by tropical basidiomycetes BABT
Moreira Neto,Sérgio Luiz; Matheus,Dácio Roberto; Machado,Kátia Maria Gomes.
The basidiomycete fungi Lentinus crinitus and Psilocybe castanella are being evaluated in a bioremediation process of soils contaminated with organochlorine industrial residues in the Baixada Santista, São Paulo. The aim of the present study was to determine the influence of pH on the fungal growth, in vitro decolorization of anthraquinonic dye Remazol Brilliant Blue R (RBBR) and laccase activity. The pH of the culture medium influenced the growth of L. crinitus and P. castanella, which presented less growth at pH 5.9 and pH 2.7, respectively. The fungi were able to modify the pH of the culture medium, adjusting it to the optimum pH for growth which was close to 4.5. Decolorization of the RBBR was maximal at a pH of 2.5 to 3.5. Higher laccase activity was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: White rot fungi; RBBR; Laccase; Peroxidases.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500003
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Laccase produced by a thermotolerant strain of Trametes trogii LK13 BJM
Yan,Jinping; Chen,Yuhui; Niu,Jiezhen; Chen,Daidi; Chagan,Irbis.
Thermophilic and thermotolerant micro-organisms strains have served as the natural source of industrially relevant and thermostable enzymes. Although some strains of the Trametes genus are thermotolerant, few Trametes strains were studied at the temperature above 30 °C until now. In this paper, the laccase activity and the mycelial growth rate for Trametes trogii LK13 are superior at 37 °C. Thermostability and organic cosolvent tolerance assays of the laccase produced at 37 °C indicated that the enzyme possessed fair thermostability with 50% of its initial activity at 80 °C for 5 min, and could remain 50% enzyme activity treated with organic cosolvent at the concentration range of 25%–50% (v/v). Furthermore, the test on production of laccase and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trametes trogii; Thermotolerant fungi; Laccase; Thermostability; Organic cosolvent tolerance.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100059
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Laccase production by Lepista sordida BJM
Cavallazzi,José Renato Pereira; Oliveira,Maria Goreti de Almeida; Kasuya,Maria Catarina Megumi.
A Lepista sordida laccase has been characterized. Laccase and manganese peroxidase were detected in liquid medium with ammonium phosphate, yeast extract and ammonium molybdidate as nitrogen sources after 3 days of cultivation. Laccase optimal temperature and pH were 45ºC and 3.5, respectively.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lepista sordida; Laccase; Enzyme.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200016
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Laccase production of white rot fungus grown on SBS paperboard coated with PET, aiming the bioadsortion strategy. Repositório Alice
RAINER, K. T.; COLOMBI, B. L.; GONÇALVES, M. J.; HELM, C. V.; TAVARES, L. B. B.; VALLE, R. de C. S. C.; VALLE, J. A. B..
bitstream/item/189960/1/2018-RAC-CrisH-ICFPB-LaccaseProduction.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Resíduo lignocelulósico; Ganoderma lucidum; Lignocellulosic wastes; Laccase.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103284
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Lignin degradation and detoxification of eucalyptus wastes by on-site manufacturing fungal enzymes to enhance second-generation ethanol yield. Repositório Alice
SCHNEIDER, W. D. H.; FONTANA, R. C.; BAUDEL, H. M.; SIQUEIRA, F. G. de; RENCORET, J.; GUTIERRZ, A.; EUGENIO, L. I. de; PRIETO, A.; MARTINEZ, M. J.; MARTINEZ, A. T.; DILLON, A. J. P.; CAMASSOLA, M..
bitstream/item/212002/1/FELIX2020Lignin-degradation-and-detoxification.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Secretome; White-rot basidiomycete; Marasmiellus palmivorus; Laccase; Saccharification; Bioethanol.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121360
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Lignin degradation, ligninolytic enzymes activities and exopolysaccharide production by Grifola frondosa strains cultivated on oak sawdust BJM
Mikiashvili,Nona A; Isikhuemhen,Omoanghe S; Ohimain,Elijah I.
Fourteen strains of Grifola frondosa (Dicks.) S. F. Gray, originating from different regions (Asia, Europe and North America) were tested for lignin degradation, ligninolytic enzyme activities, protein accumulation and exopolysaccharide production during 55 days of cultivation on oak sawdust. Lignin degradation varied from 2.6 to7.1 % of dry weight of the oak sawdust substrate among tested strains. The loss of dry matter in all screened fungi varied between 11.7 and 33.0%, and the amount of crude protein in the dry substrate varied between 0.94 to 2.55%. The strain, MBFBL 596, had the highest laccase activity (703.3 U/l), and the maximum peroxidase activity of 22.6 U/l was shown by the strain MBFBL 684. Several tested strains (MBFBL 21, 638 and 662)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Grifola frondosa; Exopolysaccharide; Laccase; Lignin degradation; Peroxidase.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300031
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Ligninases production by Basidiomycetes strains on lignocellulosic agricultural residues and their application in the decolorization of synthetic dyes BJM
Gomes,Eleni; Aguiar,Ana Paula; Carvalho,Caio César; Bonfá,Maricy Raquel B.; Silva,Roberto da; Boscolo,Mauricio.
Wood rotting Basidiomycetes collected in the "Estação Ecológica do Noroeste Paulista", São José do Rio Preto, São Paulo State, Brazil, concerning Aphyllophorales order and identified as Coriolopsis byrsina SXS16, Lentinus strigellus SXS355, Lentinus sp SXS48, Picnoporus sanguineus SXS 43 and Phellinus rimosus SXS47 were tested for ligninases production by solid state fermentation (SSF) using wheat bran or rice straw as culture media. C. byrsina produced the highest laccase (200 U mL-1) and Lentinus sp produced the highest activities of manganese peroxidase (MnP) and lignin peroxidase (LiP) (7 and 8 U mL-1, respectively), when cultivated on wheat bran. The effect of N addition on enzyme production was studied in medium containing rice straw and the data...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Laccase; Manganese peroxidase; Lignin peroxidase; Basidiomycetes; Decolorization; Synthetic dye.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100005
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Ligninolytic enzymes production and Remazol brilliant blue R decolorization by tropical brazilian basidiomycetes fungi BJM
Machado,Kátia M. G.; Matheus,Dácio R.; Bononi,Vera L. R..
Remazol Brilliant Blue R (RBBR) dye was used as substrate to evaluate ligninolytic activity in 125 basidiomycetous fungi isolated from tropical ecosystems. The extracellular RBBR decolorizing activity produced when selected fungi were grown in solid media and in soil contaminated with organochlorines was also evaluated. A total of 106 fungi decolorized the RBBR during the growth in malt extract agar (MEA, 2%); 96 fungi showed a mycelia growth and decolorization activity stronger than the P. chrysosporium used as reference. Extracellular extracts of 35 selected fungi grown on solid medium with sugar cane bagasse (BGS) were evaluated for RBBR decolorization and peroxidase activity. All fungi showed peroxidase activities, but 5 of those were unable to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: White rot fungi; Peroxidases; Laccase; MnP; Pentachlorophenol; Hexachlorobenzene.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300008
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Lignocellulolytic enzyme production of Pleurotus ostreatus growth in agroindustrial wastes BJM
Luz,José Maria Rodrigues da; Nunes,Mateus Dias; Paes,Sirlaine Albino; Torres,Denise Pereira; Silva,Marliane de Cássia Soares da; Kasuya,Maria Catarina Megumi.
The mushroom Pleurotus ostreatus has nutritional and medicinal characteristics that depend on the growth substrate. In nature, this fungus grows on dead wood, but it can be artificially cultivated on agricultural wastes (coffee husks, eucalyptus sawdust, corncobs and sugar cane bagasse). The degradation of agricultural wastes involves some enzyme complexes made up of oxidative (laccase, manganese peroxidase and lignin peroxidase) and hydrolytic enzymes (cellulases, xylanases and tanases). Understanding how these enzymes work will help to improve the productivity of mushroom cultures and decrease the potential pollution that can be caused by inadequate discharge of the agroindustrial residues. The objective of this work was to assess the activity of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Laccase; Cellulase; Mushrooms; Lignocellulolytic residues.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400035
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Lignolytic enzymes produced by Trametes villosa ccb176 under different culture conditions BJM
Yamanaka,Renata; Soares,Clarissa F.; Matheus,Dácio R.; Machado,Kátia M.G..
The expression of the enzymatic system produced by basidiomycetous fungi, which is involved in the degradation of xenobiotics, mainly depends on culture conditions, especially of the culture medium composition. Trametes villosa is a strain with a proven biotechnological potential for the degradation of organochlorine compounds and for the decolorization of textile dyes. The influence of glucose concentration, addition of a vegetable oil-surfactant emulsion, nature of the surfactant and the presence of manganese and copper on the growth, pH and production of laccase, total peroxidase and manganese-dependent peroxidase activities were evaluated. In general, acidification of the medium was observed, with the pH reaching a value close to 3.5 within the first...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Laccase; Metals; MnP; Vegetable oil; Surfactants.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100019
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Optimization of phenoxazinone synthase production by response surface methodology and its application in Congo red decolourization Electron. J. Biotechnol.
Jia,Honghua; Gao,Zhen; Ma,Yingying; Zhong,Chao; Xie,Yingchun; Zhou,Hua; Wei,Ping.
Background: Enzymatic decolourization has been recently proposed as a promising and eco-friendly method for treatment of synthetic dye-contaminated wastewaters. However, the processes require large quantities of enzymes, attracting significant attention in developing efficient methods for mass production of multifunctional enzymes. Several methods such as response surface methodology (RSM) and orthogonal experiment have been applied to optimize the parameters in bioprocesses for enzyme production. Results: In the present study, a laccase-like enzyme, phenoxazinone synthase (PHS) originated from Streptomyces antibioticus was recombinantly expressed in Escherichia coli BL21 (DE3). The production of PHS in E. coli BL21 was optimized by response surface...
Tipo: Journal article Palavras-chave: Congo red; Decolourization; Laccase; Phenoxazinone synthase; Response surface methodology.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500002
Registros recuperados: 33
Primeira ... 12 ... Última
 

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