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Natural ligands of porcine olfactory binding proteins Inra
Le Danvic, C.; Guiraudie-Capraz, G.; Abderrahmani, D.; Zanetta, J.P.; Nagnan-Le Meillour, P..
Knowledge of endogenous ligands of olfactory binding proteins is a prerequisite for studying their role in odor and pheromone transduction. Here, we report the extraction, derivatization, and characterization by gas chromatography-mass spectrometry of the natural ligands of pig, Sus scrofa (L.), Von Ebner’s Gland protein (VEG) and odorant binding protein (OBP). We identified two isoforms (VEG1 and VEG2), which differed only by the linkage of an O-N-acetylglucosamine (O-GlcNac) group on VEG1. The natural ligands of VEG1 were characterized as two isomers of testosterone, whereas ligands of VEG2 and OBP were fatty acids or their derivatives. Our findings suggest that the binding specificity of VEG1 for steroids is governed by the presence of an O-GlcNac...
Tipo: Journal Article Palavras-chave: MOLÉCULE ODORANTE ENDOGENOUS LIGAND; FATTY ACID; MALDI-TOF MASS SPECTROMETRY; ODORANT BINDING PROTEIN; OLFACTION; O-N-ACETYLGLUCOSAMINE; TESTOSTERONE; TRYTIC DIGESTION; VON EBNER'S GLAND PROTEIN.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20099a3bdd2f&uri=/notices/prodinra1/2010/11/
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The effect of environmental salinity on the proteome of the sea bass (Dicentrarchus labrax L.) Inra
Ky, C.L.; de Lorgeril, J.; Hirtz, C.; Sommerer, N.; Rossignol, M.; Bonhomme, F..
The European sea bass, Dicentrarchus labrax L., tolerates a range of salinities from freshwater to hyper-saline. To study differences in protein expression, fish were reared in both freshwater and seawater. After 3-month acclimation, gill and intestine epithelia were collected and the soluble protein extracted. In all, 362 spots were differentially expressed in the gills and intestines of fishes reared in seawater compared to those from freshwater. Fifty differential protein spots were excised from a colloidal Coomassie-stained gel. Nine separate protein spots were identified unambiguously by mass spectrometry and database searching. Among the six proteins over-expressed in gill cells in seawater, five were cytoskeleton proteins and one was the aromatase...
Tipo: Journal Article Palavras-chave: MALDI-TOF MASS SPECTROMETRY; REAL TIME PCR; SALINITY; SEA BASS; TWO-DIMENSIONAL GEL ELECTROPHORESIS.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008f070b07f&uri=/notices/prodinra1/2008/04/
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