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| Registros recuperados: 23 | |
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| Faize, M.; Brisset, M.N.; Perino, C.; Vian, B.; Barny, M.A.; Paulin, J.P.; Tharaud, M.. |
| A regulatory hrpL non-virulent mutant of Erwinia amylovora is effective in controlling fire blight disease when inoculated on apple seedlings simultaneously with the pathogenic parental strain. Mechanisms involved in this protective effect were investigated. The use of two marker genes, uidA and lacZ, expressed in the hrpL mutant and the pathogenic strain, respectively, allowed to localize simultaneously the two inoculated strains in plant tissue. An anti-beta-glucuronidase antibody was also used to detect the hrpL mutant. Both techniques indicated that the two strains localized mainly in separate areas of the leaf tissue. In addition, leaves infiltrated with the hrpL mutant exhibited a significant increase in peroxidase activity in contrast to a hrp... |
| Tipo: Journal Article |
Palavras-chave: POMMIER; FEU BACTERIEN; ERWINIA AMYLOVORA; REPONSE DE LA PLANTE; MUTANT HRP; MECANISME DE DEFENSE; PATHOGENICITE; PEROXIDASE APPLE DEFENSE RESPONSE; HISTOCHEMICAL ASSAYS. |
| Ano: 2006 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20068c412011&uri=/notices/prodinra1/2010/11/ |
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| Bakan, B.; Hamberg, M.; Perrocheau, L.; Maume, D.; Rogniaux, H.; Tranquet, O.; Rondeau, C.; Blein, J.P.; Ponchet, M.; Marion, D.. |
| Lipid transfer proteins (LTPs) are ubiquitous plant lipid-binding proteins that have been associated with multiple developmental and stress responses. Although LTPs typically bind fatty acids and fatty acid derivatives in a non-covalent way, studies on the LTPs of barley seeds have identified an abundantly occurring covalently modified form, LTP1b, the lipid ligand of which has resisted clarification. In the present study, this adduct was identified as the {alpha}-ketol 9-hydroxy-10-oxo-12(Z)-octadecenoic acid. Further studies on the formation of LTP1b demonstrated that the ligand was introduced by nucleophilic attack of the free carboxylate group of the Asp-7 residue of the protein at carbon-9 of the allene oxide fatty acid... |
| Tipo: Journal Article |
Palavras-chave: PROTEINE DE TRANSFERT DE LIPIDE; OXYLIPINE; MECANISME DE DEFENSE; REPONSE DE LA PLANTE LIPID TRANSFER PROTEIN; LTP; ALLENE OXIDE SYNTHASE. |
| Ano: 2006 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007e6451dc5&uri=/notices/prodinra1/2010/11/ |
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| Registros recuperados: 23 | |
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