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Registros recuperados: 9
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A combined proteome and transcriptome analysis of developing Medicago truncatula seeds: evidence for metabolic specialization of maternal and filial tissues Inra
Gallardo, K.; Firnhaber, C.; Zuber, H.; Héricher, D.; Belghazi, M.; Henry, C.; Küster, H.; Thompson, R..
A comparative study of proteome and transcriptome changes during Medicago truncatula (cultivar Jemalong) seed development has been carried out. Transcript and protein profiles were parallel across the time course for 50% of the comparisons made, but divergent patterns were also observed, indicative of post-transcriptional events. These data, combined with the analysis of transcript and protein distribution in the isolated seed coat, endosperm, and embryo, demonstrated the major contribution made to the embryo by the surrounding tissues. First, a remarkable compartmentalization of enzymes involved in methionine biosynthesis between the seed tissues was revealed that may regulate the availability of sulfur-containing amino acids for embryo protein synthesis...
Tipo: Journal Article Palavras-chave: SEED DEVELOPMENT; MEDICAGO TRUNCATULA; METHIONINE; METABOLISM; AMINO ACID TRANSPORTERS.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200774127ef2&uri=/notices/prodinra1/2010/09/
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Caractérisation écophysiologique de différents génotypes de Medicago truncatula au cours des phases de germination et de croissance hétérotrophe Inra
Brunel, S..
Les phases de germination et croissance hétérotrophe sont des étapes cruciales pour l’implantation d’une culture et dépendent fortement des conditions environnementales. Le cadre d’analyse fourni par le modèle de prévision des levées SIMPLE (SIMulation of PLant Emergence) a été utilisé pour la caractérisation de Medicago truncatula (M.tr.) au cours de ces étapes précoces de son cycle, en réponse à des facteurs physiques du lit de semences ayant des effets majeurs sur les levées : température, potentiel hydrique, obstacles mécaniques.M.tr est une espèce modèle. Elle a été retenue en raison de son importante diversité naturelle et de l’existence d’une core collection, permettant d’avoir accès à une certaine diversité génétique en caractérisant un nombre...
Tipo: Thesis Palavras-chave: MEDICAGO TRUNCATULA; GERMINATION; LEVEE; TEMPERATURE; POTENTIEL HYDRIQUE; OBSTACLES MECANIQUES; MODELISATION; EXPERIMENTATION NUMERIQUE; DETERMINISME GENETIQUE; PHENOTYPAGE HAUT DEBIT.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20096bf78b80&uri=/notices/prodinra1/2009/06/
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Comparison of different spatial strategies for sampling a core collection of natural populations of fodder crops Inra
Balfourier, F.; Charmet, G.; Prosperi, J.M.; Goulard, M.; Monestiez, P..
Sept stratégies d’échantillonnage d’une core collection sont comparées sur deux collections de populations naturelles d’espèces fourragères (Lolium perenne et Medicago truncatula) préalablement évaluées pour des caractéristiques agronomiques. Les cinq premières stratégies consistent en des tirages aléatoires simples ou stratifiés selon un seul niveau de classification des populations: (i) aléatoire simple (stratégie Random); (ii) stratifié selon une classification basée sur les caractéristiques agronomiques (stratégie Stratagro); (iii) stratifié selon la région ou le pays d’origine (stratégie Stratreg); (iv) stratifié selon les caractéristiques agronomiques avec contrainte de contiguïté géographique (stratégie Straconti); (v) stratifié selon les...
Tipo: Journal Article Palavras-chave: MEDICAGO TRUNCATULA; POPULATION VEGETALE; METHODE STATISTIQUE; COLLECTION BOTANIQUE; CONSERVATION DU MATERIEL GENETIQUE.
Ano: 1998 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9900002219075019&uri=/notices/prodinra1/2007/08/
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Differentiation of symbiotic cells and endosymbionts in Medicago truncatula nodulation are coupled to two transcriptome-switches Inra
Maunoury, N.; Redondo-Nieto, M.; Bourcy, M.; Van de Velde, W.; Alunni, B.; Laporte, P.; Durand, P.; Agier, N.; Marisa, L.; Vaubert, D.; Delacroix, H.; Duc, G.; Ratet, P.; Aggerbeck, L.; Kondorosi, E.; Mergaert, P..
The legume plant Medicago truncatula establishes a symbiosis with the nitrogen-fixing bacterium Sinorhizobium meliloti which takes place in root nodules. The formation of nodules employs a complex developmental program involving organogenesis, specific cellular differentiation of the host cells and the endosymbiotic bacteria, called bacteroids, as well as the specific activation of a large number of plant genes. By using a collection of plant and bacterial mutants inducing non-functional, Fix− nodules, we studied the differentiation processes of the symbiotic partners together with the nodule transcriptome, with the aim of unravelling links between cell differentiation and transcriptome activation. Two waves of transcriptional reprogramming involving the...
Tipo: Journal Article Palavras-chave: MEDICAGO TRUNCATULA; RHIZOBIUM; SYMBIOTIC MUTANT; NOD+; FIX-; CYTOLOGY; TRANSCRIPTOME.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20107d299728&uri=/notices/prodinra1/2011/05/
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Fungal lipochitooligosaccharide symbiotic signals in arbuscular mycorrhiza. Inra
Maillet, F.; POINSOT, V.; Andre, O.; PUECH-PAGES, V.; Haouy, A.; Gueunier, M.; CROMER, L.; Giraudet, D.; FORMEY, D.; Niebel, A.; MARTINEZ, E.A.; DRIGUEZ, H.; BECARD, G.; Denarié, J..
Arbuscular mycorrhiza (AM) is a root endosymbiosis between plants and glomeromycete fungi. It is the most widespread terrestrial plant symbiosis, improving plant uptake of water and mineral nutrients. Yet, despite its crucial role in land ecosystems, molecular mechanisms leading to its formation are just beginning to be unravelled. Recent evidence suggests that AM fungi produce diffusible symbiotic signals. Here we show that Glomus intraradices secretes symbiotic signals that are a mixture of sulphated and non-sulphated simple lipochitooligosaccharides (LCOs), which stimulate formation of AM in plant species of diverse families (Fabaceae, Asteraceae and Umbelliferae). In the legume Medicago truncatula these signals stimulate root growth and branching by...
Tipo: Journal Article Palavras-chave: AM; MEDICAGO TRUNCATULA; ARBUSCULAR MYCORRHIZA; SYMBIOSIS; SUSTAINABLE DEVELOPMENT; ENVIRONMENT CARBOHYDRATE SEQUENCE; CHOMATOGRAPHY HIGH PRESSURE LIQUID; DAUCUS; CAROTA CHEMISTRY METABOLISM MICROBIOLOGY; GLOMEROMYCOTA METABOLISM; LIPOPOLYSACCHARIDES CHEMISTRY METABOLISM; MEDICAGO TRUNCATULA CHEMISTRY GROWTH & DEVELOPMENT METABOLISM MICROBIOLOBY; MOLECULAR SEQUENCE DATA; MYCORRHIZAE METABOLISM; PLANT; EXTRACTS CHEMISTRY METABOLISM; PLANT ROOTS CHEMISTRY GROWTH & DEVELOPMENT METABOLISM MICROBIOLOGY; SIGNAL TRANSDUCTION; SPORES FUNGAL CHEMISTRY METABOLISM SYMBIOSIS.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD201181dabef5&uri=/notices/prodinra1/2011/07/
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How multilocus genotypic pattern helps to understand the history of selfing populations: a case study in Medicago truncatula Inra
Siol, M.; Prosperi, J.M.; Bonnin, I.; Ronfort, J..
The occurrence of populations exhibiting high genetic diversity in predominantly selfing species remains a puzzling question, since under regular selfing genetic diversity is expected to be depleted at a faster rate than under outcrossing. Fine-scale population genetics approaches may help to answer this question. Here we study a natural population of the legume Medicago truncatula in which both the fine-scale spatial structure and the selfing rate are characterized using three different methods. Selfing rate estimates were very high (B99%) irrespective of the method used. A clear pattern of isolation by distance reflecting small seed dispersal distances was detected. Combining genotypic data over loci, we could define 34 multilocus genotypes. Among those,...
Tipo: Journal Article Palavras-chave: MEDICAGO TRUNCATULA.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20093c95b76a&uri=/notices/prodinra1/2009/03/
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Microsatellite diversity and broad scale geographic structure in a model legume: building a set of nested core collection for studying naturally occurring variation in Medicago truncatula Inra
Ronfort, J.; Bataillon, T.; Santoni, S.; Delalande, M.; David, J.L.; Prosperi, J.M..
Background: Exploiting genetic diversity requires previous knowledge of the extent and structure of the variation occurring in a species. Such knowledge can in turn be used to build a corecollection, i.e. a subset of accessions that aim at representing the genetic diversity of this species with a minimum of repetitiveness. We investigate the patterns of genetic diversity and population structure in a collection of 346 inbred lines representing the breadth of naturally occurring diversity in the Legume plant model Medicago truncatula using 13 microsatellite loci distributed throughout the genome.Results: We confirm the uniqueness of all these genotypes and reveal a large amount of genetic diversity and allelic variation within this autogamous species....
Tipo: Journal Article Palavras-chave: MEDICAGO TRUNCATULA.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007b7fe3f4e&uri=/notices/prodinra1/2010/12/
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Molecular evolution and positive selection of the symbiotic gene NORK in Medicago truncatula Inra
De Mita, S.; Santoni, S.; Hochu, I.; Ronfort, J.; Bataillon, T..
Understanding the selective constraints of partner specificity in mutually beneficial symbiosis is a significant, yet largely unexplored, prospect of evolutionary biology. These selective constraints can be explored through the study of nucleotide polymorphism at loci controlling specificity. The membrane-anchored receptor NORK (nodulation receptor kinase) of the legume Medicago truncatula controls early steps of root infection by two symbiotic microorganisms: nitrogen-fixing bacteria (rhizobia) and endomycorrhizal fungi (Glomales). We analyzed the diversity of the gene NORK by sequencing 4 kilobases in 28 inbred lines sampled from natural populations. We detected 33 polymorphic sites with only one nonsynonymous change. Analysis based on Tajima’s D and Fay...
Tipo: Journal Article Palavras-chave: POLYMORPHISME SYMBIOSIS; MEDICAGO TRUNCATULA; RHIZOBIUM; NEUTRAL THEORY; POSITIVE SELECTION; NODULATION RECEPTOR KINASE.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007dfa472fb&uri=/notices/prodinra1/2010/11/
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Transcription Reprogramming during Root Nodule Development in Medicago truncatula Inra
Moreau, S.; Verdenaud, M.; Ott, T.; Letort, S.; De Billy, F.; Niebel, A.; Gouzy, J.; Niebel De Carvalho, F.; Gamas, P..
Many genes which are associated with root nodule development and activity in the model legume Medicago truncatula have been described. However information on precise stages of activation of these genes and their corresponding transcriptional regulators is often lacking. Whether these regulators are shared with other plant developmental programs also remains an open question. Here detailed microarray analyses have been used to study the transcriptome of root nodules induced by either wild type or mutant strains of Sinorhizobium meliloti. In this way we have defined eight major activation patterns in nodules and identified associated potential regulatory genes. We have shown that transcription reprogramming during consecutive stages of nodule differentiation...
Tipo: Journal Article Palavras-chave: NODULE; MEDICAGO TRUNCATULA.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011f88fd79f&uri=/notices/prodinra1/2011/07/
Registros recuperados: 9
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