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Registros recuperados: 26
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A segment and epithelium specific messenger ribonucleic acid fragment up-regulated by estradiol in the rat oviduct Biol. Res.
RIOS,MARIANA; OJEDA,SUANY; VELASQUEZ,LUIS A; MAISEY,KEVIN; CROXATTO,HORACIO B.
Estradiol accelerates oviductal embryo transport in the rat through changes of genomic expression in oviductal cells. However, the genes involved are unknown. We used a differential display by reverse transcription-polymerase chain reaction to detect estradiol (E2)-dependent genes in the rat oviduct. Rats on day 2 of pregnancy were untreated or treated with 10 µg of E2 and the oviducts were extracted at 30, 180 and 360 min later and used to isolate RNA. Products of reverse transcriptase-PCR, made with pairs of arbitrary and oligo-deoxythymidine primers, were separated on denaturing polyacrylamide gels and candidate bands were excised and reamplified. Truly positive cDNA fragments determined by a single strand conformation polymorphism assay were cloned and...
Tipo: Journal article Palavras-chave: Differential display; Estradiol; Gene; MRNA; Oviduct.
Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100011
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Changes in PEP Carboxylase, Rubisco and Rubisco activase mRNA levels from maize (Zea mays) exposed to a chronic ozone stress Biol. Res.
LEITAO,LOUIS; MAORET,JEAN-JOSÉ; BIOLLEY,JEAN-PHILIPPE.
We quantified the ozone impact on levels of Zea mays L. cv. Chambord mRNAs encoding C4-phosphoenolpyruvate carboxylase (C4-PEPc), ribulose-l,5-bisphosphate carboxylase/oxygenase small and large subunits (Rubisco-SSU and Rubisco-LSU, respectively) and Rubisco activase (RCA) using real-time RT-PCR. Foliar pigment content, PEPc and Rubisco protein amounts were simultaneously determined. Two experiments were performed to study the ozone response of the 5th and the 10th leaf. For each experiment, three ozone concentrations were tested in open-top chambers: non-filtered air (NF, control) and non-filtered air containing 40 (+40) and 80 nL L-1 (+80) ozone. Regarding the 5th leaf, +40 atmosphere induced a loss in pigmentation, PEPc and Rubisco activase mRNAs....
Tipo: Journal article Palavras-chave: Carboxylase; Maize; MRNA; Ozone; Rubisco activase.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200005
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Chronic dietary exposure to a low-Dose mixture of genistein and vinclozolin modifies the reproductive axis, testis transcriptome, and fertility Inra
Eustache, F.; Mondon, F.; Canivenc-Lavier, M.C.; Lesaffre, C.; Fulla, Y.; Berges, R.; Cravedi, J.P.; Vaiman, D.; Auger, J..
Background: The reproductive consequences and mechanisms of action of chronic exposure to low-dose endocrine disruptors are poorly understood. Objective: We assessed the effects of a continuous, low-dose exposure to a phytoestrogen (genistein) and/or an antiandrogenic food contaminant (vinclozolin) on the male reproductive tract and fertility.Methods: Male rats were exposed by gavage to genistein and vinclozolin from conception to adulthood, alone or in combination, at low doses (1 mg/kg/day) or higher doses (10 and 30 mg/kg/day). We studied a number of standard reproductive toxicology end points and also assessed testicular mRNA expression profiles using long-oligonucleotide microarrays. Results: The low-dose mixture and high-dose vinclozolin produced the...
Tipo: Journal Article Palavras-chave: ANTIANDROGEN; ENDOCRINE DISRUPTION; MALE REPRODUCTION; MRNA; PHYTOESTROGEN; SPERMATOZA; TOXICOLOGY.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20105cfb1ffd&uri=/notices/prodinra1/2011/03/
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Co-expression networks reveal potential regulatory roles of miRNAs in fatty acid composition of nelore cattle. Repositório Alice
OLIVEIRA, P. S. N. de; COUTINHO, L. L.; CESAR, A. S. M.; DINIZ, W. J. da S.; SOUZA, M. de S.; ANDRADE, B. G.; KOLTES, J. E.; MOURÃO, G. B.; ZERLOTINI NETO, A.; REECY, J. M.; REGITANO, L. C. de A..
Fatty acid (FA) content affects the sensorial and nutritional value of meat and plays a significant role in biological processes such as adipogenesis and immune response. It is well known that, in beef, the main FAs associated with these biological processes are oleic acid (C18:1 cis9, OA) and conjugated linoleic acid (CLA-c9t11), which may have beneficial effects on metabolic diseases such as type 2 diabetes and obesity. Here, we performed differential expression and co-expression analyses, weighted gene co-expression network analysis (WGCNA) and partial correlation with information theory (PCIT), to uncover the complex interactions between miRNAs and mRNAs expressed in skeletal muscle associated with FA content. miRNA and mRNA expression data were...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Ácido linoleico conjugado; Ácido oleico; Ácidos graxos; Genômica; Redes de co-expressão; Integrative genomics; MRNA; MiRNA; Bos Indicus; Gado de Corte; Beef cattle; Conjugated linoleic acid; Oleic acid.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1110820
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Differential gene expression between in vivo and in vitro maturation: a comparative study with bovine oocytes derived from the same donor pool. Repositório Alice
CAMARGO, L. S. de A.; MUNK, M.; SALES, J. N.; WOHLRES-VIANA, S.; QUINTAO, C. C. R.; VIANA, J. H. M..
bitstream/item/195183/1/jbra-23-01-0007.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: In vivo maturation; MRNA; Ovum pick-up; Gene expression.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1107759
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Down-regulation of the cardiac sarcoplasmic reticulum ryanodine channel in severely food-restricted rats BJMBR
Vizotto,V.A.; Carvalho,R.F.; Sugizaki,M.M.; Lima,A.P.; Aragon,F.F.; Padovani,C.R.; Castro,A.V.B.; Dal Pai-Silva,M.; Nogueira,C.R.; Cicogna,A.C..
We have shown that myocardial dysfunction induced by food restriction is related to calcium handling. Although cardiac function is depressed in food-restricted animals, there is limited information about the molecular mechanisms that lead to this abnormality. The present study evaluated the effects of food restriction on calcium cycling, focusing on sarcoplasmic Ca2+-ATPase (SERCA2), phospholamban (PLB), and ryanodine channel (RYR2) mRNA expressions in rat myocardium. Male Wistar-Kyoto rats, 60 days old, were submitted to ad libitum feeding (control rats) or 50% diet restriction for 90 days. The levels of left ventricle SERCA2, PLB, and RYR2 were measured using semi-quantitative RT-PCR. Body and ventricular weights were reduced in 50% food-restricted...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Food restriction; SERCA2; Ryanodine channel; Phospholamban; MRNA.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100004
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Effects of GDF-9 and FSH on mRNA expression for FSH-R, GDF-9 and BMPs in in vitro cultured goat preantral follicles BABT
Leitão,Cintia Camurça Fernandes; Costa,José Jackson do Nascimento; Brito,Ivina Rocha; Magalhães-Padilha,Déborah de Melo; Almeida,Anderson Pinto; Figueiredo,José Ricardo de; van den Hurk,Robert; Silva,José Roberto Viana.
The aim of the present study was to determine the role of GDF-9 and/or FSH on the growth and mRNA expression for FSH-R, GDF-9, and BMPs in goat secondary follicles after culture in vitro. Goat secondary follicles (~200µm) were isolated and cultured for six days in minimum essential medium (MEM) supplemented with GDF-9 (200 ng/mL), FSH (50 ng/mL) or both. At the beginning and end of culture, the follicular diameter was evaluated and compared. The levels of mRNA for GDF-9, FSH-R and BMPs -2, -4, -6, -7 and -15 in cultured follicles were quantified by real time PCR. The results showed that a significant increase of follicle diameter after six days when compared to day 0, but the presence of GDF-9 and FSH did not influence the follicular growth in comparison...
Tipo: Info:eu-repo/semantics/article Palavras-chave: BMPs; Follicles; GDF-9; In vitro; MRNA.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200007
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Exposure to toxic Alexandrium minutum activates the detoxifying and antioxidant systems in gills of the oyster Crassostrea gigas ArchiMer
Fabioux, Caroline; Sulistiyani, Yeni; Haberkorn, Hansy; Hegaret, Helene; Amzil, Zouher; Soudant, Philippe.
Harmful algal blooms of Alexandrium spp. dinoflagellates regularly occur in French coastal waters contaminating shellfish. Studies have demonstrated that toxic Alexandrium spp. disrupt behavioural and physiological processes in marine filter-feeders, but molecular modifications triggered by phycotoxins are less well understood. This study analyzed the mRNA levels of 7 genes encoding antioxidant/detoxifying enzymes in gills of Pacific oysters (Crassostrea gigas) exposed to a cultured, toxic strain of A. minutum, a producer of paralytic shellfish toxins (PST) or fed Tisochrysis lutea (T. lutea, formerly Isochrysis sp., clone Tahitian (T. iso)), a non-toxic control diet, in four repeated experiments. Transcript levels of sigma-class glutathione S-transferase...
Tipo: Text Palavras-chave: Crassostrea gigas; Alexandrium minutum; Paralytic shellfish toxins; Antioxidant enzymes; Detoxification enzymes; MRNA.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00275/38601/38623.pdf
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Expression in normals and in subjects with schizophrenia of a novel gene fragment originally isolated from monozygotic twins discordant for schizophrenia Genet. Mol. Biol.
Zumárraga,Mercedes; Andía,Isabel; Dávila,Ricardo; Miller,Jeannette C.; Friedhoff,Arnold J..
Differentially expressed clones from subtracted cDNA libraries of a pair of monozygotic twins discordant for schizophrenia have been reported in the literature. The clones were expressed in lymphocytes from the healthy twin, but not from the schizophrenic twin. In the current study, we assessed the expression of one of these clones, oksc12b, in 10 normal controls and in 10 patients who met DSM-IV criteria for schizophrenia and had never received neuroleptic medication. We hypothesized that this clone would be differentially expressed in normal controls and in the schizophrenic patients, and that its expression could be a peripheral marker of the disease. Lymphocytes were isolated and total RNA was purified, reverse-transcribed, and quantified by two PCR...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Monozygotic twins; MRNA; Schizophrenia.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100004
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Expression of Amphiregulin During the Pre- and Post-implantation Period in the Mouse Reproductive Tract OAK
LEE, Dong-Soo; YANAGIMOTO UETA, Yoshiko; SUZUKI, Hiroshi; 鈴木, 宏志.
In mammals, embryo implantation is an essential step in reproduction. Implantation is a phenomenon that involves crosstalk between the blastocyst and the maternal endometrium. However, the molecular basis of the connections between the blastocyst and endometrium is not yet fully understood. Amphiregulin is a member of the epidermal growth factor family and is known to be expressed in the luminal epithelium of the mouse uterus on 3.5 days post coitum (dpc). Thus, to clarify the mechanism of amphiregulin at fetomaternal interface, we analyzed the expression pattern of amphiregulin mRNA in the oviducts and uteri of pregnant and psuedopregnant mice by means of real-time PCR. Amphiregulin expression in the pregnant uterus dramatically increased on 2.5 dpc,...
Palavras-chave: Mice; Implantation; Embryo transfer; Amphiregulin; MRNA.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3026
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Expression of CD40 ligand, interferon-gamma and Fas ligand genes in endomyocardial biopsies of human cardiac allografts: correlation with acute rejection BJMBR
Shulzhenko,N.; Morgun,A.; Franco,M.; Souza,M.M.; Almeida,D.R.; Diniz,R.V.Z.; Carvalho,A.C.C.; Pacheco-Silva,A.; Gerbase-DeLima,M..
The purpose of the present study was to investigate the expression (mRNA) of CD40 ligand (CD40L), interferon-gamma (IFN-gamma) and Fas ligand (FasL) genes in human cardiac allografts in relation to the occurrence of acute cardiac allograft rejection as well as its possible value in predicting acute rejection. The mRNA levels were determined by a semiquantitative reverse transcriptase-polymerase chain reaction method in 39 samples of endomyocardial biopsies obtained from 10 adult cardiac transplant recipients within the first six months after transplantation. Biopsies with ongoing acute rejection showed significantly higher CD40L, IFN-gamma and FasL mRNA expression than biopsies without rejection. The median values of mRNA expression in biopsies with and...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Rejection; Cardiac allograft; Immunologic monitoring; MRNA; Gene activation; Interferon-gamma.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600013
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Expression of Uterine Sensitization-Associated Gene-1 (USAG-1) in the Mouse Uterus During the Peri-Implantation Period OAK
MAEDA, Kanami; LEE, Dong-Soo; YANAGIMOTO UETA, Yoshiko; SUZUKI, Hiroshi; 鈴木, 宏志.
Rat uterine sensitization-associated gene-1 (USAG-1) mRNA is expressed in the uterus during the peri-implantation period, and its mRNA expression in uterine epithelial cells is highest on day 5 of pregnancy. On the other hand, since changes in USAG-1 mRNA expression in the mouse uterus are not seen during the estrous cycle, USAG-1 expression might be specifically regulated by embryonic factors rather than by the maternal environment. However, the expression pattern and function of USAG-1 in the mouse uterus have not been determined. Thus, we examined the tissue-specific USAG-1 mRNA expression in the uteri of ICR mice during peri-implantation using real-time quantitative PCR. Uterine tissues, such as the myometrium, luminal epithelium, and stroma, were...
Palavras-chave: Implantation; Mouse; MRNA; Uterine sensitization-associated gene-1 (USAG-1); Uterus.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3024
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Gene expression in IFN-gamma-activated murine macrophages BJMBR
Pereira,C.A.; Modolell,M.; Frey,J.R.; Lefkovits,I..
Macrophages are critical for natural immunity and play a central role in specific acquired immunity. The IFN-gamma activation of macrophages derived from A/J or BALB/c mice yielded two different patterns of antiviral state in murine hepatitis virus 3 infection, which were related to a down-regulation of the main virus receptor. Using cDNA hybridization to evaluate mRNA accumulation in the cells, we were able to identify several genes that are differently up- or down-regulated by IFN-gamma in A/J (267 and 266 genes, respectively, up- and down-regulated) or BALB/c (297 and 58 genes, respectively, up- and down-regulated) mouse macrophages. Macrophages from mice with different genetic backgrounds behave differently at the molecular level and comparison of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene array; MRNA; Mouse hepatitis virus 3 (MHV3); IFN-gamma; BALB/c mouse; A/J mouse; Macrophages.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200005
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Gene Expression of 11β-HSD and Glucocorticoid Receptor in the Bovine (Bos taurus) Follicle During Follicular Maturation and Atresia : The Role of Follicular Stimulating Hormone OAK
TETSUKA, Masafumi; NISHIMOTO, Hiromi; MIYAMOTO, Akio; OKUDA, Kiyoshi; HAMANO, Seizo; 手塚, 雅文; 宮本, 明夫.
Glucocorticoids modulate ovarian function in cattle. However, their regulatory mechanisms have not been fully elucidated. In the present study, we examined gene expression of two glucocorticoid-metabolizing enzymes, a bidirectional 11β-HSD type 1 (11HSD1) and a dehydrogenase 11β-HSD type 2 (11HSD2), and glucocorticoid receptor (GR) in bovine follicles during follicular maturation and atresia. Granulosa cells (GCs) and theca interna layers (TIs) were harvested from follicles classified as small growing, dominant, preovulatory, early atretic and late atretic follicles. The expression levels of 11HSD1, 11HSD2 and GR mRNA were quantified by real-time PCR. In the healthy follicles, expression of 11HSD1 mRNA increased as follicles matured, both in GCs and TIs. A...
Palavras-chave: Bovine; Cortisol; 11β-HSD; Follicle; Glucocorticoid receptor; MRNA.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2810
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Identification of microRNAs and mRNAs associated with multidrug resistance of human laryngeal cancer Hep-2 cells BJMBR
Yin,Wanzhong; Wang,Ping; Wang,Xin; Song,Wenzhi; Cui,Xiangyan; Yu,Hong; Zhu,Wei.
Multidrug resistance (MDR) poses a serious impediment to the success of chemotherapy for laryngeal cancer. To identify microRNAs and mRNAs associated with MDR of human laryngeal cancer Hep-2 cells, we developed a multidrug-resistant human laryngeal cancer subline, designated Hep-2/v, by exposing Hep-2 cells to stepwise increasing concentrations of vincristine (0.02-0.96'µM). Microarray assays were performed to compare the microRNA and mRNA expression profiles of Hep-2 and Hep-2/v cells. Compared to Hep-2 cells, Hep-2/v cells were more resistant to chemotherapy drugs (∼45-fold more resistant to vincristine, 5.1-fold more resistant to cisplatin, and 5.6-fold more resistant to 5-fluorouracil) and had a longer doubling time (42.33±1.76 vs 28.75±1.12'h,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multidrug resistance; Laryngeal cancer; Hep-2 cells; MRNA; MicroRNA.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600546
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Inactivated Parapoxvirus ovis induces a transient increase in the expression of proinflammatory, Th1-related, and autoregulatory cytokines in mice BJMBR
Anziliero,D.; Weiblen,R.; Kreutz,L.C.; Spilki,F.; Flores,E.F..
The immunostimulatory properties of inactivated Parapoxvirus ovis (iPPVO) have long been investigated in different animal species and experimental settings. In this study, we investigated the effects of iPPVO on cytokine expression in mice after intraperitoneal inoculation. Spleen and sera collected from iPPVO-treated mice at intervals after inoculation were submitted to cytokine mRNA determination by real-time PCR (qPCR), serum protein concentration by ELISA, and interferon (IFN)-α/β activity by bioassay. The spleen of iPPVO-treated animals showed a significant increase in mRNA expression of all cytokines assayed, with different kinetics and magnitude. Proinflammatory cytokines interleukin (IL)-1β, tumor necrosis factor-alpha (TNF-α), and IL-8 mRNA peaked...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytokine; MRNA; ORF virus; Immunostimulant; Innate immune response.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200110
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Metabolic, productive and reproductive responses to postpartum short-term supplementation in primiparous beef cows R. Bras. Zootec.
Astessiano,Ana Laura; Pérez-Clariget,Raquel; Espasandín,Ana Carolina; López-Mazz,Carlos; Soca,Pablo; Carriquiry,Mariana.
The objective of this study was to evaluate the effect of a short-term supplementation with rice bran (2 kg/cows/day) on the endocrine and metabolic profiles and hepatic gene expression, associated with the reproductive response in beef cows in grazing conditions. Thirty-eight primiparous beef cows (Hereford, Angus and Hereford × Angus) were used in a randomized block design according to genotype, calving date and body condition score at calving (BCS). At 64±14 days postpartum (day 0 of the experiment), cows were assigned to two nutritional treatments: i) control, grazing native pastures (CON, n=19) and ii) supplemented (SUP, n = 19) for 21 days (days 1 to 21 of the experiment). Blood samples were collected at 0, 7 and 22 days and liver biopsies were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: GH-IGFI axis; Metabolism; MRNA.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000400003
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Molecular characterization of a second copy of holocarboxylase synthetase gene (hcs2) in Arabidopsis thaliana Inra
Denis, L.; Grossemy, M.; Douce, R.; Alban, C.. (Coordinateur).
Tipo: Journal Article Palavras-chave: CELLULE VEGETALE; HOLOCARBOXYLASE SYNTHETASE; EPISSAGE ALTERNATIF; BIOTINYLATION DES PROTEINES PLANTE; PLANT; PLANT CELL; HOLOCARBOXYLASE SYNTHETASE; METABOLISM; BIOCHEMISTRY; VITAMIN; BIOTIN; ENZYME; GENE EXPRESSION; MRNA; ALTERNATIVE SPLICING; PROTEIN BIOTINYLATION.
Ano: 2002 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007210a015e&uri=/notices/prodinra1/2010/11/
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Non-invasive messenger RNA transcriptional evaluation in human kidney allograft dysfunction BJMBR
Joelsons,G.; Domenico,T.; Gonçalves,L.F.; Manfro,R.C..
The aim of the present study was to evaluate messenger RNA expression in kidney allograft recipients. Forty-four kidney transplant recipients were evaluated up to three months after grafting. After transplantation, peripheral blood samples were drawn sequentially for real-time polymerase chain reaction analyses of perforin and TIM-3 genes. Biopsies were obtained to evaluate acute graft dysfunction and interpreted according to the Banff classification. Eight patients presented episodes of acute rejection. Recipients with rejection had significantly higher levels of TIM-3 mRNA transcripts compared to those without rejection (median gene expression 191.2 and 36.9 mRNA relative units, respectively; P<0.0001). Also, perforin gene expression was higher in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Kidney transplantation; Acute rejection; Gene expression; Diagnosis; MRNA.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000700608
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NPY and sbGnRH gene expression in juvenile and adult male Brazilian flounder Paralichthys orbignyanus Ciência Rural
Campos,Vinicius Farias; Collares,Tiago Veiras; Seixas,Fabiana Kömmling; Deschamps,João Carlos; Marins,Luis Fernando Fernandes; Okamoto,Marcelo Hideo; Sampaio,Luís André Nassr; Robaldo,Ricardo Berteaux.
The objective of this study was to evaluate neuropeptide Y (NPY) and sea bream gonadotropin-release hormone (sbGnRH) gene expression in juvenile and adult males of Brazilian flounder. Hypothalamuses from fish were sampled for total RNA extraction. After cDNA synthesis, real-time PCR was used to measure gene expression. NPY showed approximately 2-fold increases in their mRNA levels while sbGnRH showed 3-fold increases in adult fish. These results suggest that these peptides could be involved on hypothalamic regulation of Brazilian flounder sexual maturation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sexual maturation; Fish; Pleuronectiformes; MRNA.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011001100013
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