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Registros recuperados: 6
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Analysis of mutations in the cystic fibrosis transmembrane regulator (CFTR) gene in patients with obstructive azoospermia Genet. Mol. Biol.
Bernardino,Andrea L.F.; Lima,Cintia E.; Zatz,Mayana.
Congenital bilateral absence of the vas deferens (CBAVD) accounts for 1%-2% of sterility in men. A high incidence of mutations, as well as the involvement of the 5T variant of the T tract length in intron 8 of the cystic fibrosis conductance regulator (CFTR) gene, have been previously described in males with CBAVD. Herein we report the screening for mutations and for the 5T variant of the CFTR gene in 17 patients with CBAVD and three others with non-CABVD obstructive azoospermia. In the CBAVD group, three patients (15%) were compound heterozygotes for mutations, and five patients (25%) had a mutation in one allele and the 5T variant in the other; the 5T variant was also present in two other patients, one of them being homozygous. The most frequent mutation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CFTR mutations; Obstructive azoospermia; Male infertility.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100001
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Prevalence of Y chromosome deletions in a Brazilian population of nonobstructive azoospermic and severely oligozoospermic men BJMBR
SãoPedro,S.L.; Fraietta,R.; Spaine,D.; Porto,C.S.; Srougi,M.; Cedenho,A.P.; Avellar,M.C.W..
We determined the prevalence of Y chromosome deletions in a population of 60 Brazilian nonobstructive azoospermic and severely oligozoospermic men. PCR-based screening of microdeletions was performed on lymphocyte DNA for the presence of 14 sequence-tagged sites (STS) located in the azoospermic factor (AZF) on the Yq chromosome. All STS were amplified efficiently in samples from 12 fertile men tested, but failed to be amplified in samples from fertile women, indicating the specificity of PCR conditions for Yq screening. Overall, 4 of the 60 infertile patients tested (6.7%) exhibited deletion of the Y chromosome, 2 of them being severely oligozoospermic patients (P10 and P32) and 2 azoospermic men (patients P47 and P57). Patients P47 and P57 presented...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Y chromosome; Microdeletions; Severe oligozoospermia; Azoospermia; Male infertility; Azoospermia factor; AZF region.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000600015
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Relationship between seminal malondialdehyde levels and sperm quality in fertile and infertile men BABT
Colagar,Abasalt Hosseinzadeh; Pouramir,Mehdi; Marzony,Eisa Tahmasbpour; Jorsaraei,Sayed Gholam Ali.
The aim of this study was to determine the level of malondialdehyde in seminal plasma of fertile and infertile men and investigate its relationship with sperm quality. Results showed that the mean of ± S.D. MDA concentration in seminal plasma of infertile men (0.94 ± 0.28 nmol/ml) was significantly higher than fertile men (0.65 ± 0.17 nmol/ml) (p value< 0.001), and had negative relationship with sperm count, motility and morphology. Therefore it could be concluded that increase in lipid peroxidation was associated with sperm membrane destructed and high level of MDA.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Seminal plasma; Male infertility; Sperm quality; Malondialdehyde (MDA); Lipid peroxidation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600010
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Somatic and germ cell cytogenetic studies and AZF microdeletion screening in infertile men Genet. Mol. Biol.
Carrara,Rita C. V.; Yamasaki,Rui; Mazucatto,Luís F.; Veludo,Maria A. Llorach; Sartorato,Edi L.; Pina-Neto,João M..
Clinical and cytogenetic studies were performed in 65 infertile individuals, and 56 of them were also screened for microdeletions in Yq11 (AZF region). Relevant environmental etiological factors were identified in 10 cases (15.4%). Sertoli-cell-only syndrome was diagnosed in six patients (9,2%). Karyotype abnormalities were detected in six individuals, and five other patients presented desynapsis of bivalents in meiosis. Three out of the 56 patients studied were carriers of microdeletions in the AZF region, one of them also presenting a chromosomal mosaicism for an extra i(22p).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Male infertility; Cytogenetics; Microdeletions; AZF; Meiotic anomalies.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400002
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Somatic cytogenetic and azoospermia factor gene microdeletion studies in infertile men BJMBR
Pina-Neto,J.M.; Carrara,R.C.V.; Bisinella,R.; Mazzucatto,L.F.; Martins,M.D.; Sartoratto,E.; Yamasaki,R..
The objective of the present study was to determine the frequency of somatic chromosomal anomalies and Y chromosomal microdeletions (azoospermia factor genes, AZF) in infertile males who seek assisted reproduction. These studies are very important because the assisted reproduction techniques (mainly intracytoplasmic sperm injection) bypass the natural selection process and some classical chromosomal abnormalities, microdeletions of AZF genes or some deleterious genic mutations could pass through generations. These genetic abnormalities can cause in the offspring of these patients male infertility, ambiguous external genitalia, mental retardation, and other birth defects. We studied 165 infertile men whose infertility was attributable to testicular problems...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetics; Y microdeletions; Male infertility; Karyotype; Chromosome aberrations.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400017
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Sperm Morphological Studies of the West African Dwarf Buck Treated with Pumpkin Plant (Cucurbita pepo) International Journal of Morphology
Oyeyemi,M. O; Olukole,S. G; Esan,O.
Four healthy bucks of the West African Dwarf breed aged between 24 and 30 months were used for this study. The bucks were first used as control and later as experimental animals upon being fed daily with oiled pumpkin plant for the period of six months. The objective of the study was to investigate the effect of the pumpkin plant on the morphology of the spermatozoa of the bucks. There were significant differences (p< 0.05) between the control and experimental values for both primary and secondary morphological spermatozoa abnormalities: the pyriform head has a control value of 6 (0. 42%) and post-feeding value of 0 (0%), the beat tail; 14(0.97%) and 2 (0.16%) for the control and post feeding values respectively. The curved mid piece: 17 (1.18%) and 1...
Tipo: Journal article Palavras-chave: Pumpkin plant; Morphology; Male infertility; Spermatozoa.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000100020
Registros recuperados: 6
Primeira ... 1 ... Última
 

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