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A di-O-dihydrogeranylgeranyl glycerol from Thermococus S 557, a novel ether lipid, and likely intermediate in the biosynthesis of diethers in Archaea ArchiMer
Gonthier, Isabelle; Rager, Marie-noëlle; Metzger, Pierre; Guezennec, Jean; Largeau, Claude.
The lipids of a deep-sea hydrothermal vent arch ae on, Thermococcus S 557, were isolated, purified and structurally determined. Based on acid methanolysis and spectroscopic studies, the polar lipids were shown to comprise diphytanyl glycerol and dibiphytanyl diglycerol, typical membrane lipids of Arch aea. From the neutral lipids, 2,3-di-O-dihydro-14,15-geranylgeranyl glycerol was isolated. This novel ether lipid is, very likely, a close intermediate in the biosynthesis of diphytanyl glycerol diether in Arch aea.
Tipo: Text Palavras-chave: Mass spectrometry; NMR; Novel glycerol diether lipid; Thermococcus sp; Archaea.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-736.pdf
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A new scenario of bioprospecting of Hymenoptera venoms through proteomic approach J. Venom. Anim. Toxins incl. Trop. Dis.
Santos,LD; Pieroni,M; Menegasso,ARS; Pinto,JRAS; Palma,MS.
Venoms represent a huge and essentially unexplored reservoir of bioactive components that may cure diseases that do not respond to currently available therapies. This review select advances reported in the literature from 2000 to the present about the new scenario of Hymenoptera venom composition. On account of new technologies in the proteomic approach, which presents high resolution and sensitivity, the combination of developments in new instruments, fragmentation methods, strategic analysis, and mass spectrometry have become indispensable tools for interrogation of protein expression, molecule interaction, and post- translational modifications. Thus, the biochemical characterization of Hymenoptera venom has become a major subject of research in the area...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hymenoptera; Venoms; Allergens; Phospholipase A1; Wasp venom antigen 5; Hyaluronoglucosaminidase; Mass spectrometry; Proteomics.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400003
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A sensitive bioanalytical method development and validation of cabozantinib in human plasma by LC-ESI-MS/MS BJPS
Inturi,Srikanth; Avula,Prameela Rani.
ABSTRACT A simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of cabozantinib (CZ) in human plasma using cabozantinib-d4 (CZD4) as an internal standard (IS). Chromatographic separation was performed on Xbridge C18, 50 x 4.6 mm, 5 mm column with an isocratic mobile phase composed of 10mM Ammonium formate and Methanol in the ratio of (20:80 v/v), at a flow-rate of 0.7 mL/min. CZ and CZD4 were detected with proton adducts at m/z 502.2 ® 391.1 and 506.3 ® 391.2 in multiple reaction monitoring (MRM) positive mode respectively. Liquid-Liquid extraction method was used to extract the drug and IS. The method was validated over a linear concentration range of 5.0-5000.0 pg/mL with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cabozantinib/human plasma/validation; Mass spectrometry; Pharmacokinetic/study.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200622
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Biochemical survey of the polar head of plant glycosylinositolphosphoceramides unravels broad diversity ArchiMer
Cacas, Jean-luc; Bure, Corinne; Furt, Fabienne; Maalouf, Jean-paul; Badoc, Alain; Cluzet, Stéphanie; Schmitter, Jean-marie; Antajan, Elvire; Mongrand, Sébastien.
Although Glycosyl-Inositol-Phospho-Ceramides (GIPCs) are the main sphingolipids of plant tissues, they remain poorly characterized in term of structures. This lack of information, notably with regard to polar heads, currently hampers the understanding of GIPC functions in biological systems. This situation prompted us to undertake a large scale-analysis of plant GIPCs: 23 plant species chosen in various phylogenetic groups were surveyed for their total GIPC content. GIPCs were extracted and their polar heads were characterized by negative ion MALDI and ESI mass spectrometry. Our data shed light on an unexpected broad diversity of GIPC distributions within Plantae, and the occurrence of yet-unreported GIPC structures in green and red algae. In monocots,...
Tipo: Text Palavras-chave: Glycosphingolipid; Glycosyl-Inositol-Phospho-Ceramide; GIPC; Mass spectrometry; MALDI-TOF; Polar head; Roots; Photosynthetic tissues; Berries.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00166/27689/25884.pdf
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Bufotenine is able to block rabies virus infection in BHK-21 cells J. Venom. Anim. Toxins incl. Trop. Dis.
Vigerelli,Hugo; Sciani,Juliana Mozer; Jared,Carlos; Antoniazzi,Marta Maria; Caporale,Graciane Maria Medeiros; Silva,Andréa de Cássia Rodrigues da; Pimenta,Daniel C.
Background Rabies is a fatal zoonotic neglected disease that occurs in more than 150 countries, and kills more than 55.000 people every year. It is caused by an enveloped single stranded RNA virus that affects the central nervous system, through an infection initiated by the muscular nicotinic acetylcholine receptor, according to many authors. Alkaloids, such as acetylcholine, are widespread molecules in nature. They are present in numerous biological fluids, including the skin secretion of many amphibians, in which they act (together with proteins, peptides and steroids) as protection agents against predators and/or microorganisms. Among those amphibians that are rich in alkaloids, there is the genus Rhinella.Methods Bufotenine was isolated from Rhinela...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rabies; Rhinella; Bufotenine; Alkaloids; Toxins; Mass spectrometry.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200336
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Calmodulin gene as good voucher as MALDI-TOF ICMS to identify Portuguese isolates of Aspergillus section Flavi IPB - Escola Superior Agrária
Rodrigues, Paula; Santos, Cledir; Venâncio, Armando; Lima, Nelson.
Aspergillus is a large genus, with a complex and ever evolving taxonomy. Section Flavi is one of the most significant Sections in the Genus. Taxonomy and species identification is subject of great interest for scientists aiming to clarify the species concept and limits within the section. Furthermore, this Section comprises both toxigenic and non-toxigenic species/strains, with great interest to biotechnology and food industry. Various genes, namely the rRNA (internal transcribed spacer (ITS) and partial LSU-rRNA regions), calmodulin and ~-tubulin genes, have been widely reported as good markers for Aspergillus species identification, because they are rapid and cost-effective.
Tipo: ConferenceObject Palavras-chave: Fungal identification; DNA sequencing; Mass spectrometry.
Ano: 2010 URL: http://hdl.handle.net/10198/4276
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Can the hemoglobin characteristics of vesicomyid clam species influence their distribution in deep-sea sulfide-rich sediments? A case study in the Angola Basin ArchiMer
Decker, Carole; Zorn, N.; Le Bruchec, J.; Caprais, Jean-claude; Potier, N.; Leize-wagner, E.; Lallier, F. H.; Olu, Karine; Andersen, A. C..
Vesicomyids live in endosymbiosis with sulfur-oxidizing bacteria and therefore need hydrogen sulfide to survive. They can nevertheless live in a wide range of sulfide and oxygen levels and depths, which may explain the exceptional diversity of this clam family in deep-sea habitats. In the Gulf of Guinea, nine species of vesicomyid clams are known to live in cold-seep areas with pockmarks from 600 to 3200 m deep, as well as in the organic-rich sediments of the Congo deep-sea fan at 5000 m deep. Our previous study showed that two species living in a giant pockmark have different oxygen carriers, suggesting different adaptations to hypoxia. Here, we studied the hemoglobin structure and oxygen affinity in three other species, Calyptogena valdiviae, Elenaconcha...
Tipo: Text Palavras-chave: Hemoglobin; Mass spectrometry; Oxygen affinity; Symbiont-bearing bivalve; Blood-clams; Cold seeps; Sulfide-rich sediments.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00358/46961/46875.pdf
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Challenges in proteome analyses of tropical plants Braz. J. Plant Physiol.
Balbuena,Tiago S.; Dias,Leonardo L. C.; Martins,Mariana L. B.; Chiquieri,Tatiana B.; Santa-Catarina,Claudete; Floh,Eny I. S.; Silveira,Vanildo.
Genome sequencing of various organisms allow global analysis of gene expression, providing numerous clues on the biological function and involvement in the biological processes studied. Proteomics is a branch of molecular biology and biotechnology that has undergone considerable development in the post-genomic era. Despite the recent significant advancements in proteomics techniques, still there is much to be improved. Due to peculiarities to the plant kingdom, proteomics approaches require adaptations, so as to improve efficiency and accuracy of results in plants. Data generated by proteomics can substantially contribute to the understanding and monitoring of plant physiological events and development of biotechnological strategies. Especially for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2-DE; Mass spectrometry; Recalcitrant species; Proteomics; Unknown genome.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000200001
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Characterization and differentiation of aflatoxigenic species of Aspergillus section Flavi by MALDI-TOF MS IPB - Escola Superior Agrária
Rodrigues, Paula; Kallow, W.; Erhard, M.; Welker, M.; Kozakiewickz, Zofia; Lima, Nelson; Venâncio, Armando.
Aspergillus is a large genus, with a complex taxonomy. The genus is easily identifiedd by its characteristic conidiophore, but species identification and differentiation is complex, mainly because it is traditionally based on a range of morphological features. Aspergillus subgenus Circumdati section Flavi, also refered to as the A.dflavus group, has attracted worldwide attention for its industrial use and toxigenic potential. Sectiond Flavi is divided in two groups of species. One includes the aflatoxigenic species A. flavus, A. parasiticus and A. nomius, which cause serious problems in agricultural commodities, and the other one includes the non-aflatoxigenic species A. oryzae, A. sojae and A.dtamarii, traditionally used for production of fermented foods....
Tipo: ConferenceObject Palavras-chave: Aflatoxigenic fungi; Mass spectrometry; Aspergillus flavus; Aspergillus parasiticus.
Ano: 2007 URL: http://hdl.handle.net/10198/5759
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Characterization and extraction of volatile compounds from pineapple (Ananas comosus L. Merril) processing residues Ciênc. Tecnol. Aliment.
Barretto,Lília Calheiros de Oliveira; Moreira,Jane de Jesus da Silveira; Santos,João Antônio Belmino dos; Narendra,Narain; Santos,Raquel Anne Ribeiro dos.
The aim of this study was to extract and identify volatile compounds from pineapple residues generated during concentrated juice processing. Distillates of pineapple residues were obtained using the following techniques: simple hydrodistillation and hydrodistillation by passing nitrogen gas. The volatile compounds present in the distillates were captured by the solid-phase microextraction technique. The volatile compounds were identified in a system of high resolution gas chromatography system coupled with mass spectrometry using a polyethylene glycol polar capillary column as stationary phase. The pineapple residues constituted mostly of esters (35%), followed by ketones (26%), alcohols (18%), aldehydes (9%), acids (3%) and other compounds (9%)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agro-industrial residues; High resolution gas chromatography; Mass spectrometry.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000400007
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Characterization of New Oligosaccharides Obtained by An Enzymatic Cleavage of the Exopolysaccharide Produced by the Deep-Sea Bacterium Alteromonas infernus Using its Cell Extract ArchiMer
Akoumany, Katy; Zykwinska, Agata; Sinquin, Corinne; Marchand, Laetitia; Fanuel, Mathieu; Ropartz, David; Rogniaux, Hélène; Pipelier, Muriel; Delbarre Ladrat, Christine; Colliec Jouault, Sylvia.
Bacteria from deep-sea hydrothermal vents constitute an attractive source of bioactive molecules. In particular, exopolysaccharides (EPS) produced by these bacteria become a renewable source of both biocompatible and biodegradable molecules. The low molecular weight (LMW) derivatives of the GY785 EPS produced by the deep-sea hydrothermal vent strain Alteromonas infernus have previously displayed some biological properties, similar to those of glycosaminoglycans (GAG), explored in cancer and tissue engineering. These GAG-mimetic derivatives are obtained through a free radical depolymerization process, which could, however, affect their structural integrity. In a previous study, we have shown that A. infernus produces depolymerizing enzymes active on its own...
Tipo: Text Palavras-chave: Deep-sea bacterium; Alteromonas infernus; Wild-type strain; Exopolysaccharides; Glycosaminoglycan-mimetic; Enzymatic depolymerization; Structural analysis; Mass spectrometry.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00515/62644/67021.pdf
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Characterization of new phenolic derivatives in Portuguese propolis by electrospray mass spectroscopy IPB - Escola Superior Agrária
Falcão, Soraia; Vilas-Boas, Miguel; Freire, C.; Domingues, M.R.M.; Cardoso, Susana M..
This research outlines an extensive characterization of the phenolic composition of a propolis sample from the northeast of Porto gal. For that, ao ethanolic extract was prepared, fractionated by HPLC and the identification of the phenolic compounds was done by electro spray mass spectrometry in the negative mode. This technical approach allowed the identification of 37 phenolic compounds in the Portoguese propolis sample, including seven that were described for the first time. Two of these new compounds had [M-Hr ions at mlz 403, aod the others had [M-Hr ions at mlz 433, mlz 461, mlz 417, mlz 475, and mlz 565. In general, the molecular weight of these compounds was higher thao the common phenolic compounds of propolis aod their fragmentation pattern...
Tipo: ConferenceObject Palavras-chave: Propolis; Phenolic compounds; Mass spectrometry; Electrospray ionization.
Ano: 2009 URL: http://hdl.handle.net/10198/5265
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Comparative analysis of the venom proteome of four important Malaysian snake species J. Venom. Anim. Toxins incl. Trop. Dis.
Vejayan,Jaya; Khoon,Too Lay; Ibrahim,Halijah.
BackgroundNaja kaouthia, Ophiophagus hannah, Bungarus fasciatusandCalloselasma rhodostomaare four venomous snakes indigenous to Malaysia. In the present study, their proteomic profile by two-dimensional gel electrophoresis (2-DE) have been separated and compared.ResultsThe 2-DE of venoms of the four species snake demonstrated complexity and obvious interspecies differences in proteome profiles. A total of 63 proteins were identified in the four species: C. rhodostoma -26, N. kaouthia -16, O. hannah -15 and B. fasciatus -6.ConclusionsDespite the identifications of major proteins in the four snake species, a large number of protein spots from the 2-DE were unidentified even though the spots displayed high-quality MALDI-TOF-MS spectra. Those identified...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mass spectrometry; Snake venoms; Proteomics; Two-dimensional gel electrophoresis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200317
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Convenient preparation of picolinyl derivatives from fatty acid esters ArchiMer
Dubois, Nolwenn; Barthomeuf, Chantal; Berge, Jean-pascal.
It is essential to have simple rapid methods for the determination of fatty acid structures. Traditionally, fatty acids are analysed by gas chromatography using their methyl ester derivatives (FAME). However, their corresponding mass spectra exhibit molecular ions but are usually devoid of ions indicative of structural features and, notably, the position of double bounds on the aliphatic chains [1]. In the most useful approach to structure determination, the carboxyl group is derivatised with a reagent containing a nitrogen atom. Recently, a convenient method for preparing picolinyl esters from intact lipids has been published [2]. However, some problems occurred in our laboratory when this method was used, leading to some modifications and optimisation....
Tipo: Text Palavras-chave: Sensitivity; Sterol esters; Fatty acid methyl esters; Gas chromatography; Mass spectrometry; Picolinyl esters.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2125.pdf
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Criblage bioguidé et analyse CL - SMHR des toxines et d'au tres métabolites d'intérêt chez les dinoflagellés G ambierdiscus et Fukuyoa ArchiMer
Pisapia, Francesco.
The epi-benthic dinoflagellates Gambierdiscus and Fukuyoa produce ciguatoxins (CTXs) and maitotoxins (MTXs), which are among the most potent marine toxins known. CTXs are bio-accumulated and bio-transformed along the marine food chain and cause Ciguatera Fish Poisoning (CFP), the most common non-bacterial foodborne intoxication worldwide. Recently, CFP has been reported from areas previously not considered endemic, namely the Canary Islands, and an increasing number of species has been discovered in these genera. Little is known about the relative toxicity between different species and the toxin congeners produced by these dinoflagellates, especially by strains outside the Pacific Ocean. Several strains of Gambierdiscus and Fukuyoa were screened for their...
Tipo: Text Palavras-chave: Ciguatéra; Gambierdiscus; Fukuyoa; Ciguatoxines; Maïtotoxines; Test neuro-2a; Test hémolytique; Spectrométrie de masse.; Keywords: Ciguatera Fish Poisoning; Gambierdiscus; Fukuyoa; Ciguatoxins; Maitotoxins; Neuro-2a assay; Hemolytic assay; Mass spectrometry.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00415/52635/53481.pdf
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Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers ArchiMer
Geiger, Marie; Guitton, Yann; Vansteelandt, M.; Kerzaon, I.; Blanchet, Estelle; Du Pont, T. Robiou; Frisvad, J. C.; Hess, Philipp; Pouchus, Y. F.; Grovel, Olivier.
In order to assess the putative toxigenic risk associated with the presence of fungal strains in shellfish-farming areas, Penicillium strains were isolated from bivalve molluscs and from the surrounding environment, and the influence of the sample origin on the cytotoxicity of the extracts was evaluated. Extracts obtained from shellfish-derived Penicillia exhibited higher cytotoxicity than the others. Ten of these strains were grown on various media including a medium based on mussel extract (Mytilus edulis), mussel flesh-based medium (MES), to study the influence of the mussel flesh on the production of cytotoxic compounds. The MES host-derived medium was created substituting the yeast extract of YES medium by an aqueous extract of mussel tissues, with...
Tipo: Text Palavras-chave: Cytotoxicity; Dereplication; Marine fungi; Mass spectrometry; Penicillium; Shellfish.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00160/27166/25523.pdf
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Detection and identification of huwentoxin-IV interacting proteins by biotin-avidin chemistry combined with mass spectrometry J. Venom. Anim. Toxins incl. Trop. Dis.
Yu,Hai; Liu,Hui; Yan,Yizhong; Duan,Zhigui; Wang,Xianchun.
Background : Numerous spider toxins are of interest as tools for neurophysiological research or as lead molecules for the development of pharmaceuticals and insecticides. Direct detection and identification of the interacting proteins of a spider toxin are helpful for its action-mechanism analysis and practical application. The present study employed a combinative strategy for the analysis of interacting proteins of huwentoxin-IV (HWTX-IV), a peptidic neurotoxin from the venom of the spiderSelenocosmia huwena.Results : HWTX-IV was first lightly labeled with biotin under the optimized mild experimental conditions and the toxin labeled with a single biotin group (monobiotinylated HWTX-IV) was demonstrated by electrophysiological experiments to retain its...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotin labeling; Huwentoxin-IV; Far-western blotting; Interacting protein; Mass spectrometry.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200328
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Determinação de agrotóxicos e ocratoxina A em café arábica sob diferentes níveis de torrefação. Infoteca-e
ROSA, J. S. da; FREITAS-SILVA, O.; CAMPOS, R. da S.; CASTRO, I. M. de; TEIXEIRA, A. da S..
O café é a segunda maior commodity do mundo e tamanha importância faz do café uma matriz alimentar muito estudada. A necessidade de incremento na produtividade dessa cultura, porém, pode aumentar o uso de alguns agrotóxicos em sistemas de produção de café. Da mesma forma, as práticas de colheita e processamento pós-colheita do café estão diretamente relacionadas à contaminação dos grãos por fungos micotoxigênicos. Rastrear a presença destes dois tipos de contaminantes em amostras de café Arábica comum (com diferentes teores de grãos defeituosos) e submetidos a diferentes níveis de torra foi o objetivo deste trabalho. Os agrotóxicos foram analisados pelo método multirresíduo, por espectrometria de massas sequencial, e a ocratoxina A, por cromatografia...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Contaminante; Cromatografia líquida de alta eficiência; Micotoxina; Coffea Arábica; Tecnologia de Alimento; Espectrometria; Cromatografia; Food technology; Vegetable products; High performance liquid chromatography; Mycotoxins; Mass spectrometry; Poisoning; Agrochemicals.
Ano: 2019 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1112112
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Determinação de resíduos de paclobutrazol em manga (Mangifera indida L.) por cromatografia acoplada a espectometria de massas (LC-MS/MS). Infoteca-e
FERRACINI, V. L.; QUEIROZ, S. C. do N. de; ROSA, M. A.; LOPES, P. R. C..
Paclobutrazol (PBZ) é um regulador de crescimento que tem sido utilizado para alterar o ciclo fenológico da mangueira visando a produção de frutos em qualquer época do ano. É bastante utilizado na região nordeste do Brasil e a determinação dos resíduos na fruta é importante pois há pouco conhecimen- to sobre o acúmulo desta substância na planta em condições brasileiras. Para isso foi necessário adaptar um método analítico para determinar resídu- os de paclobutrazol em frutos de manga. Após a extração do PBZ na matriz o extrato foi analisado por LC-MS/MS. Os limites de detecção (LOD) e de quantificação (LOQ) obtidos para o PBZ foram de 0,25 ?g Kg-1 e 1,25 ?g Kg-1, respectivamente. As recuperações foram de 82 a 94% e se encontram dentro da faixa considerada...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: LC-MS/MS; Resíduos de paclobutrazol; Especrometria.; Resíduo químico; Regulador de crescimento; Análise química; Cromatografia; Manga.; Paclobutrazol; Chemical residues; Chemical analysis; Chromatography; Mass spectrometry.
Ano: 2011 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/898423
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Direct identification of bovine mastitis pathogens by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in pre-incubated milk BJM
Barreiro,Juliana R.; Gonçalves,Juliano L.; Grenfell,Rafaella; Leite,Renata F.; Juliano,Luiz; Santos,Marcos V..
ABSTRACT The present study aimed to compare two MALDI-TOF identification methods [(a) direct sample identification after pre-incubation; or (b) use of bacteria isolated on pre-culture)] to standard, traditional bench microbiology. A total of 120 quarter milk samples from 40 Holstein lactating cows were screened based on culture-positive results obtained by microbiological culture (reference method) with the following numbers of quarters positive per cow: 4 cows with 1, 8 cows with 2, 12 cows with 3 and 16 cows with 4 infected quarters per cow. For direct identification method, quarter milk samples (n = 120) were skimmed by centrifugation (10,000 × g/10 min) and pre-incubated at 37 ºC for 12 h. After pre-incubation, quarter milk samples were submitted to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Milk; Bacteria; Mastitis; Mass spectrometry.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400801
Registros recuperados: 49
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