Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 58
Primeira ... 123 ... Última
Imagem não selecionada

Imprime registro no formato completo
A SURVEY OF SHEEP MASTITIS IN SOUTHERN BRAZIL Ciência Rural
Vaz,Adil Knackfuss; Jones,James Eirug Thomas.
SUMMARY Ovine mastitis causes varying economic fosses in all countries where sheep are kept. A survey of 3128 breeding ewes in 22 farms in two areas with different management practices in Southern Brazil showed that the average prevalence of subclinical and chronic mastitis was 14.1% of ewes with at least one affected gland. From those, 4.49% were also bacteriologically positive. The prevalence in individual farms varied from 0 to 37% of ewes with clinical abnormalities of the udder. The overall prevalence was greater in Area II, where meat breeds predominate and animals are housed at night. It is concluded that subclinical and chronic mastitis is uniikely to be an economic problem in Area I, where wool production is the objective of the sheep industry....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sheep; Ewes; Mastitis; Lambs; Brazil.
Ano: 1994 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781994000200022
Imagem não selecionada

Imprime registro no formato completo
Accurate identification of atypical Staphylococcus chromogenes plasma-clotting strains causing bovine mastitis Ciência Rural
Avellar-Costa,Pedro; Santos,Danielle Cabral dos; Lange,Carla Christine; Laport,Marinella Silva; Brito,Maria Aparecida Vasconcelos Paiva; Rossi,Ciro César; Giambiagi-deMarval,Marcia.
ABSTRACT: We compared the potential of routine techniques used for the identification of Staphylococcus species, aiming to evaluate their accuracy in the detection of 43 Staphylococcus chromogenes strains isolated from bovine mastitis that, despite being a coagulase-negative species, are able to clot plasma. These strains could be mistakenly suspected to be S. aureus and lead to an unappropriated treatment of the disease. MALDI-TOF, PCR-RFLP of the chaperonine gene groEL, and sequencing of the 16S rRNA and elongation factor Tu gene tuf were employed. Results from the four methods were coincident for only half of the strains because of the low accuracy of the groEL PCR-RFLP (51.2% accuracy). Even though all the sequencing results were identical, the high...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus chromogenes; Mastitis; MALDI-TOF; Tuf; GroEL.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000600452
Imagem não selecionada

Imprime registro no formato completo
Actividad antibacteriana de los extractos de Syzygium cumini (L.) Skeels (jambolán) frente a los microorganismos asociados a la mastitis bovina Plantas Medicinales
Voigt Mota,Fernanda; Damé Schuch,Luiz Filipe; Lambrecht Gonçalves,Carolina; Faccin,Ângela; Almeida Schiavon,Diane Bender; Conrad Bohm,Bianca; Ferreira Lessa,Lisiane.
Introducción: la aparición de cepas bacterianas resistentes a los antibióticos ha estimulado el desarrollo de nuevas líneas de investigación, basadas en las propiedades antibacterianas de plantas utilizadas en medicina popular. La medicina veterinaria ha estado tomando para sí los beneficios de la medicina tradicional, en un intento de sustituir el uso de antibióticos en el tratamiento y la prevención de la mastitis. Objetivo: evaluar la actividad antibacteriana de los extractos hidralcohólicos de Syzygium cumini (L.) Skeels, en comparación con las bacterias relacionadas en la mastitis bovina. Métodos: se obtuvieron extractos de hojas frescas con alcohol a 92,8° y de hojas secas con alcohol a 70°. La actividad antimicrobiana de plantas se evaluó utilizando...
Tipo: Journal article Palavras-chave: Planta medicinal; Syzygium cumini; Extractos hidroalcohólicos; Mastitis.
Ano: 2013 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962013000300016
Imagem não selecionada

Imprime registro no formato completo
Actividad antimicrobiana de aceite esencial de Origanum vulgare L. ante bacterias aisladas en leche de bovino Plantas Medicinales
Bastos Oyarzabal,Marta Elaine; Damé Schuch,Luiz Filipe; de Souza Prestes,Luciana; Bender Almeida Schiavon,Diane; Alves Rodrigues,Maria Regina; Braga de Mello,João Roberto.
Introducción: la mastitis bovina es el mayor problema en la producción lechera, causada principalmente por bacterias grampositivas. La búsqueda de principios activos que actúen en esos microrganismos es creciente, sobre todo por la ocurrencia de multirresistencia bacteriana. Objetivos: evaluar la concentración bactericida mínima del aceite esencial de Origanum vulgare L. (orégano) frente a bacterias aisladas de leche mastítica. Métodos: se evaluó la actividad antimicrobiana del aceite esencial de O. vulgare frente a 71 bacterias aisladas de leche bovina, de los géneros Streptococcus, Staphylococcus y Corynebacterium; y 3 cepas patrón de Pseudomonas aeruginosa, Staphylococcus aureus y Escherichia coli. La técnica utilizada fue de dilución en microplaca....
Tipo: Journal article Palavras-chave: Origanum vulgare; Mastitis; Antimicrobiano.
Ano: 2011 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962011000300006
Imagem não selecionada

Imprime registro no formato completo
Aplicación de plantas medicinales para la antisepsia de pezones de vacas posordeño Plantas Medicinales
Almeida Schiavon,Diane Bender; Damé Schuch,Luiz Filipe; Bastos Oyarzabal,Marta Elaine; de Souza Prestes,Luciana; Zani,João Luiz; de Andrade Hartwig,Carla.
Introducción: el sistema de producción de leche agroecológico está limitado por alternativas sostenibles de promoción a la salud animal. El control de la mastitis con plantas medicinales puede ser viable de modo económico y ecológicamente. Objetivos: evaluar la aplicación de antisépticos en la desinfección de pezones posordeño. Métodos: se comparó un convencional y extracto de plantas medicinales en un rebaño comercial y tuvo como principal encierre las nuevas infecciones intramamarias. Resultados: la prevalencia semanal del California Mastitis Test varió en el grupo 1 entre 29,5 y 17,1 %, y en el grupo 2 de 29,7 a 19,6 %, no diferenciándose de modo significativo, en ninguna de las semanas. La incidencia de cultura positiva para...
Tipo: Journal article Palavras-chave: Mastitis; Plantas medicinales; Tagetes minuta.
Ano: 2011 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962011000300005
Imagem não selecionada

Imprime registro no formato completo
APLICACIÓN DE UN MODELO MÚLTIPLE DE CURVA DE LACTANCIA PARA VACAS LECHERAS Agricultura Técnica
Pedraza G.,Carlos; Mansilla M.,Alberto; Jahn B.,Ernesto; Vidal V.,Agustín; Fajardo R.,Paola; Agüero E.,Hernán.
El objetivo del presente trabajo fue probar una nueva expresión matemática destinada a mejorar la interpretación de los recuentos de células somáticas y niveles de producción de leche. Un modelo múltiple de curva de lactancia se comparó con el modelo clásico de Wood, separadamente para cada uno de los rangos celulares (RC). Se trabajó con una base de datos depurada proveniente del control sistemático de cinco rebaños lecheros que representan una masa de 1200 vacas Holstein Friesian. Se ajustó una curva de producción de leche, separadamente para vaquillas y vacas, mediante la expresión y(t) = a t b e- c t. El modelo múltiple propuesto estuvo basado en la ecuación anterior, incorporando la variable RC en la forma y (t, RC = a t b e- c t e d RC + f RC2. El...
Tipo: Journal article Palavras-chave: Modelo matemático; Células somáticas; Producción láctea; Mastitis.
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072002000400003
Imagem não selecionada

Imprime registro no formato completo
Association of the occurrence of some diseases with reproductive performance and milk production of dairy herds in southern Brazil R. Bras. Zootec.
Scheid Filho,Virgilio Balduíno; Schiavon,Raquel Schiavon; Gastal,Gustavo Desire Antunes; Timm,Cláudio Dias; Lucia Jr.,Thomaz.
This study analyzed associations between the occurrence of mastitis, metritis and retained placenta with the reproductive performance and milk production of four Holstein dairy herds of southern Brazil. Calving-to-conception intervals (CCI) and daily milk production were compared across herds, number of artificial inseminations and clinical occurrences and occurrence of mastitis, metritis and retained placenta. The CCI of cows with three or more clinical occurrences was longer than for those with no occurrences, but shorter for cows with retained placenta than for those without it. Cows with clinical mastitis up to 60 days post-partum had shorter CCI than those with mastitis after 60 days. Cows with retained placenta also had higher frequency of metritis....
Tipo: Info:eu-repo/semantics/other Palavras-chave: Dairy; Mastitis; Production; Retained placenta.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000200033
Imagem não selecionada

Imprime registro no formato completo
Association of TLR4 polymorphisms with subclinical mastitis in Brazilian holsteins BJM
Mesquita,Adriano Queiroz de; Rezende,Cintia Silva Minafra e; Mesquita,Albenones José de; Jardim,Eurione Antonio Garcia da Veiga; Kipnis,Ana Paula Junqueira.
The identification of dairy cows with greater or lower potential to develop mastits has been pursued for many years among different segments of the milk industry, including governmental organizations. Genomic studies have suggested that Single Nucleotide Polymorphisms (SNPs) within the pattern recognition receptors (PRR) could lead to different responses to pathogens, and consequently result in mastitis resistance or susceptibility. To investigate whether toll like receptor 4 (TLR4) gene is associated with subclinical mastitis in Holstein cows from a property in the state of Goiás, Brazil, TaqMan allelic discrimination and somatic cell count were performed. One hundred and fifty milk samples were analyzed for SCC and centesimal composition. Twenty percent...
Tipo: Info:eu-repo/semantics/article Palavras-chave: TLR-4; Single nucleotide polymorphism; Mastitis; Milk quality.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200034
Imagem não selecionada

Imprime registro no formato completo
Avaliação do teor de cloretos e da condutividade elétrica no leite de cabra como métodos auxiliares de diagnóstico na mastite subclínica caprina. Infoteca-e
SOUZA, V. de; FACCIOLI-MARTINS, P. Y.; MOURA, J. W. F.; FERNANDES, D. R.; LIMA, A. R..
Esta publicação avalia os testes de detecção do teor de cloretos e da condutividade elétrica (CE) em relação ao isolamento de Staphylococcus spp.
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Diagnosis.; Doença animal; Teste de mamite; Staphylococcus aureus; Caprino; Diagnóstico; Cloreto; Condutividade elétrica; Animal diseases; Mastitis; Goat milk; Chlorides; Electrical conductivity..
Ano: 2017 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1086974
Imagem não selecionada

Imprime registro no formato completo
Bacteriocins: exploring alternatives to antibiotics in mastitis treatment BJM
Pieterse,Reneé; Todorov,Svetoslav D..
Mastitis is considered to be the most costly disease affecting the dairy industry. Management strategies involve the extensive use of antibiotics to treat and prevent this disease. Prophylactic dosages of antibiotics used in mastitis control programmes could select for strains with resistance to antibiotics. In addition, a strong drive towards reducing antibiotic residues in animal food products has lead to research in finding alternative antimicrobial agents. In this review we have focus on the pathogenesis of the mastitis in dairy cows, existing antibiotic treatments and possible alternative for application of bacteriocins from lactic acid bacteria in the treatment and prevention of this disease.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mastitis; Antibiotic; Milk; Bacteriocin; Food safety.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300003
Imagem não selecionada

Imprime registro no formato completo
Biochemical characteristics of typical and atypical Staphylococcus aureus in mastitic milk and environmental samples of Brazilian dairy farms BJM
Silva,Wladimir Padilha da; Destro,Maria Teresa; Landgraf,Mariza; Franco,Bernadette D.G.M..
This study reports results on the biochemical characteristics of 274 Staphylococcus aureus strains isolated from mastitic milk, hands of milkmen and milking machines. Features included colony morphology on Baird Parker agar (BPA), catalase, coagulase and thermonuclease tests. API-Staph (bioMérieux, France) was used as reference identification system. 39.4% of the colonies on BPA identified as S.aureus were atypical. They were more frequent in milk samples (94.4%). All S.aureus strains were coagulase positive but intensity of the test varied according to the source: environmental strains were 3+ or 4+, while 17.1% of strains isolated from milk presented only 1+ or 2+ results. The majority of atypical S.aureus strains presented a 4+ coagulase result. 2.2% of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Atypical S.aureus; Coagulase; Thermonuclease; API-Staph; Baird-Parker agar; Milk; Mastitis; Environmental samples.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200008
Imagem não selecionada

Imprime registro no formato completo
Biofilm production and beta-lactamic resistance in Brazilian Staphylococcus aureus isolates from bovine mastitis BJM
Marques,Viviane Figueira; Motta,Cássia Couto da; Soares,Bianca da Silva; Melo,Dayanne Araújo de; Coelho,Shana de Mattos de Oliveira; Coelho,Irene da Silva; Barbosa,Helene Santos; Souza,Miliane Moreira Soares de.
Abstract Staphylococcus spp. play an important role in the etiology of bovine mastitis. Staphylococcus aureus is considered the most relevant species due to the production of virulence factors such as slime, which is required for biofilm formation. This study aimed to evaluate biofilm production and its possible relation to beta-lactamic resistance in 20 S. aureus isolates from bovine mastitic milk. The isolates were characterized by pheno-genotypic and MALDI TOF-MS assays and tested for genes such as icaA, icaD, bap, agr RNAIII, agr I, agr II, agr III, and agr IV, which are related to slime production and its regulation. Biofilm production in microplates was evaluated considering the intervals determined along the bacterial growth curve. In addition, to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilm; Agr types; Antimicrobial resistance; Mastitis.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100118
Imagem não selecionada

Imprime registro no formato completo
Bovine mastitis in the metropolitan area of Curitiba: antibiotic resistance and antimicrobial control of the infection BABT
Parada,José Luis; Gonçalves,Dicezar; Soccol,Vanete Tomaz; Lima,Marisa; Soccol,Carlos Ricardo.
A study from cows with mastitis was performed and Staphylococcus aureus was the predominant pathogen in 46.4 % among 153 studied strains from 276 milk samples of infected cows. Antibiotic resistance of 71 S. aureus isolates was determined in order to search resistant strains to antibiotics of clinical interest, as well as to determine their degree of multi-resistance. It was found that 60% of the S. aureus strains presented resistance to β-lactams, but none to oxacillin, teicoplamin or vancomycin. On the other hand, with the aim of reducing the use of current antibiotics and their associated resistance, a new formulation was introduced. The antimicrobial compounds (P22-P32), demonstrated to be effective in 55% of the 76 mastitis cases studied. The use of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mastitis; Staphylococcus aureus; Antibiotic resistance; Infection reduction.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400009
Imagem não selecionada

Imprime registro no formato completo
Characterization of Staphylococcus spp. strains in milk from buffaloes with mastitis in Brazil: the need to identify to species level to avoid misidentification Arq. Bras. Med. Vet. Zootec.
Coimbra-e-Souza,V.; Brito,M.A.V.P.; Chamon,R.C.; Laport,M.S.; Giambiagi-deMarval,M..
ABSTRACT Mastitis is an inflammation of the mammary gland that affects dairy cattle worldwide causing economic losses. Coagulase-negative staphylococci (CNS) are the predominant cause of this type of infection. We have recently showed that coagulase-positive staphylococci could be misidentified. So, the aim of this study was to characterize the Staphylococcus spp. strains initially classified as coagulase-negative Staphylococci, isolated from buffalo with subclinical mastitis. Milk of buffaloes with mastitis in herds was collected and 9 strains were identified as CNS by phenotypic tests. Molecular methodologies latter identified the strains as coagulase-negative Staphylococcus chromogenes (5), coagulase-positive Staphylococcus hyicus (2) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Buffalo; Mastitis; Staphylococcus; Milk; Coagulase test.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000601669
Imagem não selecionada

Imprime registro no formato completo
Clinical features of caprine arthritis-encephalitis infections Arq. Bras. Med. Vet. Zootec.
Lara,M.C.C.S.H; Birgel Junior,E.H.; Gregory,L.; Birgel,E.H..
In a clinical survey of caprine arthritis-encephalitis in 14 naturally infected herds in the State of São Paulo, Brazil, 17.1% (64/374) of seroreagents presented the articular form of the disease and 6.6% (17/249) of the seroreagent females presented the mammary form of the disease.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caprine; Arthritis- encephalitis; Mastitis.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600005
Imagem não selecionada

Imprime registro no formato completo
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk BJM
Martins,Katheryne Benini; Faccioli-Martins,Patricia Yoshida; Riboli,Danilo Flávio Moraes; Pereira,Valéria Cataneli; Fernandes,Simone; Oliveira,Aline A.; Dantas,Ariane; Zafalon,Luiz Francisco; Cunha,Maria de Lourdes Ribeiro de Souza da.
The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mastitis; Sheep; Staphylococcus aureus; Antibiotic resistance; Virulence factors.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535
Imagem não selecionada

Imprime registro no formato completo
Comparative analysis of agr groups and virulence genes among subclinical and clinical mastitis Staphylococcus aureus isolates from sheep flocks of the Northeast of Brazil BJM
Almeida,Lara M. de; Almeida,Mayra Zilta P.R.B. de; Mendonça,Carla L. de; Mamizuka,Elsa M..
Staphylococcus aureus is one of the most frequent mastitis causative agents in small ruminants. The expression of most virulence genes of S. aureus is controlled by an accessory gene regulator (agr)locus. This study aimed to ascertain the prevalence of the different agr groups and to evaluate the occurrence of encoding genes for cytotoxin, adhesins and toxins with superantigen activity in S. aureus isolates from milk of ewes with clinical and subclinical mastitis in sheep flocks raised for meat production The agr groups I and II were identified in both cases of clinical and subclinical mastitis. Neither the arg groups III and IV nor negative agr were found. The presence of cflA gene was identified in 100% of the isolates. The frequency of hla and lukE-D...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Agr groups; Virulence genes; Mastitis; Sheep flock.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200026
Imagem não selecionada

Imprime registro no formato completo
Contagem de células somáticas e california mastitis test no diagnóstico da mastite caprina subclínica. Infoteca-e
SILVA, E. R. da; SAUKAS, T. N.; ALVES, F. S. F.; PINHEIRO, R. R..
A mastite e uma doenca altamente prevalente em rebalnhos leiteiros de todo o mundo. Os agentes causais das mastites em caprinos sao semelhantes aqueles da doenca em bovinos, sendo os Staphylococcus coagulase positiva os mais frequentes patogenos associados a forma clinica e os Staphylococcus coagulase negativa a forma subclinica da mastite caprina. O diagnostico da mastite clinica e facil, baseado primariamente nos sinais clinicos; entretanto, o diagnostico da forma subclinica e um problema, tendo-se como metodos auxiliares os testes fisico-quimicos do leite e os baseados no aumento do conteudo celular da glandula mamaria. Por serem rapidos e de facil execucao, os testes baseados no aumento celular do leite sao muito utilizados, sendo o California Mastitis...
Tipo: Documentos (INFOTECA-E) Palavras-chave: Diagnostic.; Caprino; Mamite; Diagnóstico; Goats; Mastitis; California mastitis test..
Ano: 1996 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/514270
Imagem não selecionada

Imprime registro no formato completo
CONTROL INTERNO DE LA CALIDAD APLICADO AL CENMAST, PRODUCTO PARA DETECTAR MASTITIS Rev Salud Anim.
Betancourt,A; Ramírez,Anledys; Navarro,Verónica; González,Daisy; López,Yanet; Linares,Anel.
El CENMAST es un diagnosticador con un principio semejante a la prueba de California, durante su producción por el Centro Nacional de Sanidad Agropecuaria, el laboratorio de control de la calidad comprueba las especificaciones del producto, con las determinaciones del principio activo, pH y densidad, cuyos resultados están bajo un programa de control interno de la calidad. Se evaluó un lote del diagnosticador como material de referencia de trabajo, para el uso como muestra de control de la calidad, también se empleó el mismo en la validación de la precisión de los ensayos en condiciones de repetibilidad y condiciones intermedias variando los analistas, día de trabajo, equipos y la calibración. Se halló una adecuada precisión entre las réplicas, se...
Tipo: Journal article Palavras-chave: Control interno de la calidad; Diagnosticador; Mastitis.
Ano: 2010 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2010000300004
Imagem não selecionada

Imprime registro no formato completo
Controle integrado de mastite bovina. Infoteca-e
BRITO, J. R. F.; BRESSAN, M..
bitstream/item/132469/1/Livro-Controle-integrado-da-mastite-bovina.pdf
Tipo: Livro técnico (INFOTECA-E) Palavras-chave: Mastite; Integrated control.; Controle Integrado; Leite.; Mastitis; Milk..
Ano: 1996 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/592224
Registros recuperados: 58
Primeira ... 123 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional