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Recherche et caractérisation d'immunoglobulines dans les oeufs du loup (Dicentrarchus labrax) ArchiMer
Pepin, Jean-francois; Breuil, Gilles; Rigobert, P; Romestand, Bernard.
The occurrence of maternal immunity transfer in Sea bass (Dicentrarchus labrax) was investigated using ELISA and Western blot technics. In order to determine the possible presence of immunoglobulin (Ig), egg proteins obtained from fertilized eggs were analyzed. After several extraction protocol assays (precipitation, chromatography), monoclonal and polyclonal antibody probes specific to Sea bass Ig allowed to confirm the presence of an immunoglobulin-like protein in eggs with very low level. Biochemical characterization was carried out using SDS-PAGE. Results indicated that egg Ig had a molecular weight of 206 kDa. They were smaller than seric IgM (800 kDa). The heavy (H) and light (L) chains of the egg Ig and of the seric IgM had similar molecular weight,...
Tipo: Text Palavras-chave: Immunoglobuline; Transfert immunité maternelle; ELISA; Œuf; Poisson; Immunoglobulin; Maternal immunity transfer; ELISA; Eggs; Fish.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00272/38275/36458.pdf
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