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A simple method for isolation of genomic DNA from fresh and dry leaves of Terminalia arjuna (Roxb.) Wight and Arnot Electron. J. Biotechnol.
Deshmukh,Vishal P; Thakare,Prashant V; Chaudhari,Uddhav S; Gawande,Prashant A.
Current protocols for isolation of genomic DNA from Terminalia arjuna have their own limitations due to the presence of high content of gummy polysaccharides and polyphenols. DNA isolated by these protocols is contaminated with a yellowish, sticky and viscous matrix. In our modified DNA isolation method polysaccharides and polyphenols are removed prior to the precipitation of the DNA. Then the genomic DNA was precipitated using isopropanol. This protocol yielded a high molecular weight DNA isolated from fresh as well as dry leaves of T. arjuna, which was free from contamination and colour. Isolated DNA can be used for restriction digestion and PCR amplification. The main objective of the present protocol is to provide a simple method of isolation of DNA,...
Tipo: Journal article Palavras-chave: Ayurveda; DNA extraction; Medicinal plant; Terminalia species.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300014
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Absence of mutagenicity in somatic and germ cells of mice submitted to subchronic treatment with an extract of Croton cajucara Benth. (Euphorbiaceae) Genet. Mol. Biol.
Santos,Fabio V.; Mesquita,Suzana F.P.; Faria,Maria José S.S.; Poersh,Aline; Maciel,Maria Aparecida M.; Pinto,Angelo C.; Morimoto,Helena K.; Cólus,Ilce Mara S..
The plant Croton cajucara Benth. (Euphorbiaceae) is a medicinal plant from the Brazilian Amazon where it is commonly known as sacaca. The principal compound isolated from C. cajucara stem-bark extracts is the clerodane-type diterpene trans-dehydrocrotonin (DCTN) which presents several biological activities, including antiulcerogenic, anti-inflammatory, hypoglycemic, antimutagenic and antitumoral activity. However, few studies have been carried out to evaluate the therapeutic potential of raw C. cajucara extracts. We studied mutagenicity and antimutagenicity effects of C. cajucara methanol extract using the micronucleus assay in bone marrow cells and the dominant lethal assay in mice submitted to subchronic treatments. The blood testosterone levels of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimutagenicity; Mutagenicity; Dominant lethal; Micronucleus; Croton cajucara; Medicinal plant.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100029
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Action of Aqueous Extracts of Phyllanthus niruri L. (Euphorbiaceae) leaves on Meristematic Root Cells of Allium cepa L. Anais da ABC (AABC)
NEVES,ERASMOVLANE S.B.; FERREIRA,PAULO MICHEL PINHEIRO; LIMA,LEONARDO H.G.M.; PERON,ANA PAULA.
This study aimed to evaluate the effects of aqueous extracts of dried Phyllanthus niruri L. (stonebreaker) leaves on Allium cepa L. root meristem cells at four concentrations, 0.02 (usual concentration), 0.04, 0.06 and 0.08mg/mL and exposure times of 24 and 48 hours. For each concentration we used a group of five onion bulbs that were first embedded in distilled water and then transferred to their respective concentrations. The radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were prepared by the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5000 for each control and exposure time. The calculated mitotic indices were subjected to the Chi-squared statistical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plant; Stonebreaker; Cell division; Cellular aberrations; Allium cepa.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301131
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Active phloem sap collection by Trigona spinipes (Fabricius) (Hymenoptera, Apidae) in Artemisia annua Linn (Asteraceae) Biological Sciences
Malagodi-Braga, Kátia Sampaio; Moriconi, Waldemore; Queiroga, Joel Leandro de; Urchei, Mário Artemio; Pazianotto, Ricardo Antônio Almeida; Roncon, Kenny.
Trigona spinipes Fabricius (Apidae: Meliponini), is a common stingless bee widely seen in urban and rural areas in Brazil, popularly known as irapuá, arapuá or bee-dog. Although these bees are considered pollinators of some cultivated plants, they are better known for the damage they cause in different crops. During experimental agroecological cultivation of Artemisia (Artemisia annua Linn, Asteraceae), in Jaguariúna (SP, Brazil), stingless bees Trigona spinipes (Fabricius) were observed sucking phloem sap directly from the plant, a phenomenon not yet described in scientific literature. This study aimed to register and describe the phloem sap-sucking behavior performed by T. spinipes for the first time, as well as to assess the potential impact of this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plant; Insect behavior; Plant injury; Stingless bee Farming..
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/47548
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Alterations in the mitotic index of Allium cepa induced by infusions of Pluchea sagittalis submitted to three different cultivation systems Anais da ABC (AABC)
Rossato,Liana V.; Tedesco,Solange B.; Laughinghouse IV,Haywood D.; Farias,Júlia G.; Nicoloso,Fernando T..
We evaluated the antiproliferative effect of infusions from Pluchea sagittalis using the Allium cepa test. Infusions in three concentrations (2.5, 5, and 25 g dm-3) of leaves cultivated in three environments (in vitro, acclimatized growth chamber, and field) were used. Six onion bulbs were used for each of the eight treatments, and the mitotic index was obtained from 6000 cells per treatment. In conclusion, leaf infusions of P. sagittalis cultivated in the field have a high antiproliferative activity, as well as the cultivation system influences the antiproliferative potential.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antiproliferative effect; Camphorweed; Cell cycle; Medicinal plant; Mutagenicity.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000400007
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Antibacterial effects of Thymus algeriensis extracts on some pathogenic bacteria Biological Sciences
Messaoudi, Mohammed; Benreguieg, Mokhtar; Merah, Maroua; Messaoudi, Zakarya Ayoub.
Despite the presence of many antibiotics, bacterial resistance is growing steadily and some of these antibiotics have become ineffective, which poses a major challenge to the health sector. In this context, this work has demonstrated, in vitro, the inhibitory action of the bacterial growth resulting from methanolic and ethanolic extracts of Thymus algeriensis Boiss. & Reut., a medicinal plant species harvested from the Algerian South-west area, as well as the determination of the phenolic content of those crude extracts. The methanolic extract of Thymus algeriensis showed a significant antibacterial effect with 16.5 and 19 mm against Pseudomonas aeruginosa and Staphylococcus aureus, respectively. Klebsiella pneumoniae was not inhibited by both...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibacterial effect; Extracts; Medicinal plant; Phenolic content; Thymus algeriensis..
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/48548
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Antifungal activities of the essential oil and its fractions rich in sesquiterpenes from leaves of Casearia sylvestris Sw. Anais da ABC (AABC)
PEREIRA,FLAVIANE G.; MARQUETE,RONALDO; DOMINGOS,LEVY T.; ROCHA,MARCO E.N.; FERREIRA-PEREIRA,ANTONIO; MANSUR,ELISABETH; MOREIRA,DAVYSON L..
ABSTRACT Casearia genus (Salicaceae) is found in sub-tropical and tropical regions of the world and comprises about 160-200 species. It is a medicinal plant used in South America, also known as “guaçatonga”, “erva-de-tiú”, “cafezinho-do-mato”. In Brazil, there are about 48 species and 12 are registered in the State of Rio de Janeiro, including Casearia sylvestris Sw. There are many studies related to the chemical profile and cytotoxic activities of extracts from these plants, although few studies about the antifungal potential of the essential oil have been reported. In this work, we have studied the antifungal properties of the essential oil of C. sylvestris leaves, as well as of their fractions, against four yeasts (Saccharomyces cerevisae, Candida...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida sp.; Casearia genus; Essential oil; Medicinal plant; Saccharomyces cerevisae..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602817
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Antimicrobial activity of Anonna mucosa (Jacq.) grown in vivo and obtained by in vitroculture BJM
de Souza Barboza,Thiago José; Ferreira,Andréa Fonseca; de Paula Rosa Ignacio,Ana Claudia; Albarello,Norma.
Brazilian flora includes numerous species of medicinal importance that can be used to develop new drugs. Plant tissue culture offers strategies for conservation and use of these species allowing continuous production of plants and bioactive substances. Annona mucosa has produced substances such as acetogenins and alkaloids that exhibit antimicrobial activities. The widespread use of antibiotics has led to an increase in multidrug-resistant bacteria, which represents a serious risk of infection. In view of this problem, the aim of this work was to evaluate the antibacterial potential of extracts of A. mucosa obtained by in vitro techniques and also cultured under in vivo conditions. Segments from seedlings were inoculated onto different culture media...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibacterial properties; Annonaceae; Medicinal plant; Callus cultures; Cell suspension cultures.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300785
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Antinociceptive effect of the ethanolic extract of Amburana cearensis (Allemão) A.C. Sm., Fabaceae, in rodents. Repositório Alice
OLIVEIRA, R. R. B.; GÓIS, R. M. O.; SIQUEIRA, R. S.; ALMEIDA, J. R. G. S.; LIMA, J. T.; NUNES, X. P.; OLIVEIRA, V. R. de; SILQUEIRA, J. S.; QUINTANS JÚNIOR, L. J..
Efeito antinociceptivo do extrato etanólico de Amburana cearensis (Allemão) A.C. Sm., Fabaceae, em roedores.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Umburana de cheiro; Fabaceae; Atividade antinociceptiva; Amburana cearensis; Planta medicinal; Medicinal plant.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/657921
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Antiparasitic activity, histopathology and physiology of Colossoma macropomum (tambaqui) exposed to the essential oil of Lippia sidoides (Verbenaceae). Repositório Alice
SOARES, B. V.; NEVES, L. R.; FERREIRA, D. O.; OLIVEIRA, M. S. B.; CHAVES, F. C. M.; CHAGAS, E. C.; GONÇALVES, R. A.; TAVARES-DIAS, M..
In vivo and in vitro antiparasitic activity of the essential oil of Lippia sidoides and blood and histological alterations were assessed in Colossoma macropomum (tambaqui). Essential oil concentrations of 10, 20, 40, 80, 160 and 320 mg/L were assayed in vitro against monogenoideans Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri from fish gills. Lippia sidoides essential oil concentrations of 320 and 160 mg/L were 100% effective against monogenoideans in 10 min and 1 h of exposure, respectively. However, the effectiveness of 100% concentrations of 80 mg/L and 40 mg/L occurred in 3 and 6 h, respectively. In the in vivo tests, juvenile fish were submitted to 60 min of baths with 10 mg/L and 15 min of baths with 20 mg/L of the...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Peixe de água doce; Parasito; Sangue; Planta medicina; Freshwater fish; Parasite; Blood; Medicinal plant.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1066533
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Antiparasitic activity of the essential oil of Lippia alba on ectoparasites of Colossoma macropomum (tambaqui) and its physiological and histopathological effects. Repositório Alice
SOARES, B. V.; NEVES, L. R.; OLIVEIRA, M. S. B.; CHAVES, F. C. M.; DIAS, M. K. R.; CHAGAS, E. C.; TAVARES-DIAS, M..
This study investigated the in vivo and in vitro antiparasitic effects of the essential oil of Lippia alba and the blood-related and histopathological alterations that it causes in Colossoma macropomum.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Monogenoideans; Parasites; Medicinal plant; Blood; Colossoma macropomum; Parasita; Óleo essencial; Plantas medicinais; Lippia alba; Peixe de água doce; Plantas medicinais; Tambaqui.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1028308
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Antiparasitic activity of the essential oil of Lippia origanoides (Verbenaceae) in native freshwater fish Colossoma macropomum. Repositório Alice
GONÇALVES, B. B.; SOARES, B. V.; CARDOSO, A. C. F.; SANTOS, G. G.; TAVARES-DIAS, M..
bitstream/item/165064/1/CPAF-AP-2017-Antparasitic-activity-of-the-essential-oil.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Peixe de água doce; Tambaqui; Parasito de animal; Planta medicinal; Hematologia; Freshwater fish; Animal parasite; Medicinal plant; Blood.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076290
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Antiparasitic, physiological and histological effects of the essential oil of Lippia origanoides (Verbenaceae) in native freshwater fish Colossoma macropomum. Repositório Alice
SOARES, B. V.; CARDOSO, A. C. F.; CAMPOS, R. R.; GONÇALVES, B. B.; SANTOS, G. G.; CHAVES, F. C. M.; CHAGAS, E. C.; TAVARES-DIAS, M..
This study examined the in vitro and in vivo, histopathological, anti-parasitic and hematopathological effects of the essential oil of Lippia origanoides on Colossoma macropomum. Essential oil concentrations of 10, 20, 40, 80, 160 and 320 mg·L-1 were tested in vitro against monogenoideans (Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri) from the gills of C. macropomum. Concentrations of 320 and 160 mg·L-1 were 100% effective against these parasites within 20 and 60 min of exposure, respectively. The 80 mg·L-1 concentration was approximately 80% effective with 3 h of exposure, reaching 100% with 6 h of exposure. The 40 mg·L-1 concentration was also 100% effective with 6 h of exposure. The other concentrations were only...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Peixe de água doce; Parasito; Sangue; Planta medicinal; Freshwater fish; Parasite; Blood; Medicinal plant.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1059330
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Antiplatelet but not anticoagulant activity of Morinda citrifolia L. leaf extracts Plantas Medicinales
Ramírez Beltrán,Roberto; García Mir,Vivian; Armenteros Herrera,Dulce María; Quintela Pena,Ana María; Garcia-Mesa,Milagros.
Tipo: Journal article Palavras-chave: Morinda citrifolia L; (Noni); Medicinal plant; Platelet aggregation; Blood coagulation; Flavonoid; Cuba.
Ano: 2015 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962015000200008
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Antiproliferative action of aqueous extracts of Hymenaea stigonocarpa Mart. (Fabaceae) on the cell cycle of Allium cepa L. Anais da ABC (AABC)
LACERDA,LOURRAN P.; MALAQUIAS,GEIZ; PERON,ANA PAULA.
In this study we evaluated the action of crude aqueous extracts obtained from rhytidome of Hymenaea stigonocarpa (jatobá-do-cerrado) on Allium cepa meristematic root cells in three concentrations: 0.082, 0.164, 0.328g/mL, at exposure times of 24 and 48 h. The slides were prepared by the crushing technique, and cells analyzed throughout the cell cycle, totaling 5000 for each control group and concentration. It was found that all three concentrations, including the lowest which is considered ideal for use, in all exposure times, had significant antiproliferative action on the cell cycle of this test system. For cells under division, we observed a high number of cells in prophase. Therefore, under the conditions studied H. stigonocarpa indicated to be...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allium cepa; Cellular division; Cells in prophase; Medicinal plant.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301147
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Antiproliferative and genotoxic effects of Mikania glomerata (Asteraceae) Biocell
Dalla Nora,Gracieli; Pastori,Tamara; Dail Laughinghouse IV,Haywood; Do Canto-Dorow,Thais Scotti; Bosio Tedesco,Solange.
Mikania glomerata is a plant used in Brazilian traditional medicine, known as ‘guaco'. It possesses anti-inflammatory properties and the aqueous extracts of its leaves are indicated for the treatment of diseases of the respiratory tract. This study aimed at evaluating the antiproliferative and genotoxic effect of Mikania glomerata leaf infusions on the cell cycle of onion. The material used was collected in the native environment from Rio Grande do Sul State, Brazil. Aqueous extracts through infusions were prepared in two concentrations: 4g/L (usual concentration) and 16g/L (4x more concentrated) of each of the populations. Two groups of four onion bulbs for each plant population were used plus a control group. The rootlets were fixed in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plant; Allium cepa test; Chromosomal aberration.
Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000300001
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Antiulcerogenic activity of Indigofera truxillensis Kunth Biota Neotropica
Cola-Miranda,Maira; Barbastefano,Victor; Hiruma-Lima,Clélia Akiko; Calvo,Tamara Regina; Vilegas,Wagner; Brito,Alba Regina Monteiro Souza.
The genus Indigofera (Fabaceae) is used in folk medicine to treat gastrointestinal pain. In this study, we investigated the antiulcerogenic properties of Indigofera truxillensis Kunth. Oral administration of MeOH extract did not produce any signals of acute toxicity. The antiulcerogenic activity was assessed in different models of acute gastric ulcers (100% ethanol, piroxicam 30 mg.kg-1, hypothermic restraint stress and pylorus ligature) in mice and rats. The animals were treated with the drugs lanzoprazole (30 mg.kg-1) or cimetidine (100 mg.kg-1) as positive controls depending on the performed model. In another experiment with ethanol-induced ulcers in rats, N-ethylmaleimide (NEM), a sulfhydryl group blocker, was also used. The MeOH extract, at doses of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antiulcerogenic activity; Fabaceae; Indigofera truxillensis Kunth; Medicinal plant; Phenolic compounds.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032006000300004
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Artisanal extraction and traditional knowledge associated with medicinal use of Crabwood Oil (Carapa guianensis Aublet.) in a peri-urban Várzea environment in the Amazon Estuary. Repositório Alice
NARDI, M.; LIRA-GUEDES, A. C.; CUNHA, H. F. A.; GUEDES, M. C.; MUSTIN, K.; GOMES, S. C. P..
Varzea forests of the Amazon estuary contain species of importance to riverine communities. For example, the oil extracted from the seeds of crabwood trees is traditionally used to combat various illnesses and as such artisanal extraction processes have been maintained. The objectives of this study were to (1) describe the process involved in artisanal extraction of crabwood oil in the Fazendinha ProtectedArea, in the state ofAmap´a; (2) characterise the processes of knowledge transfer associated with the extraction and use of crabwood oil within a peri-urban riverine community; and (3) discern medicinal uses of the oil.The data were obtained using semistructured interviews with 13 community members involved in crabwood oil extraction and via direct...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Andiroba; Carapa guianensis; Planta medicinal; Medicinal plant; Amazon.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1049042
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Assessment of mint (Mentha spp.) species for large-scale production of plantlets by micropropagation. Repositório Alice
GOMES, H. T.; BARTOS, P. M. C.; MARTINS, A. E.; OLIVEIRA, S. O. D. de; SCHERWINSKI-PEREIRA, J. E..
2015
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Hortelã; Propagação in vitro; Peppermint; Menta; Planta medicinal; Medicinal plant; In vitro propagation.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1038000
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Assessment of the cytotoxic, genotoxic, and antigenotoxic activities ofCeltis iguanaea (Jacq.) in mice Anais da ABC (AABC)
BORGES,FLAVIO F.V.; MACHADO,THIAGO C.; CUNHA,KÊNYA S.; PEREIRA,KARLA C.; COSTA,ELSON A.; PAULA,JOSÉ R. DE; CHEN-CHEN,LEE.
Ethnobotanical surveys of Cerrado native plants show that leaves of Celtis iguanaea (Jacq.) Sargent (Cannabaceae), popularly known in Brazil as “esporão de galo”, are used in folk medicine for body pain, asthma, cramps, poor digestion, urinary infection, kidney dysfunctions, as well as a stimulant and diuretic. This work aimed at evaluating possible C. iguanaea aqueous leaf extract (CALE) cytotoxicity, genotoxicity, and antigenotoxicity using the mouse bone marrow micronucleous test. To assess CALE genotoxicity, Swiss mice were orally treated with three different extract concentrations (100, 300, and 500 mgkg−1). To evaluate its antigenotoxicity, the same doses were used simultaneously with a single i.p. dose of mitomycin C (MMC, 4mg.kg−1). The frequencies...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytotoxicity; Genotoxicity; Mice; Medicinal plant.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000300955
Registros recuperados: 129
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