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Activity and composition of the benthic fauna in the Whittard Canyon and the adjacent continental slope (NE Atlantic) ArchiMer
Duineveld, Gérard; Lavaleye, Marc; Berghuis, Eilke; De Wilde, Peter.
We compared the sediment and its community on the Celtic continental slope (Goban Spur) with those in a branch of the nearby Whittard Canyon in search for evidence of canyon mediated transport of (labile) organic matter. We studied the megabenthos and macrobenthos biomass and taxonomic composition, measured in situ sediment community oxygen consumption and determined sediment concentrations of particulate organic carbon, phytopigments, and nucleic acids. While the sediment community and activity on the canyon fan was similar to that on the abyssal station near Goban Spur, the sediment within the canyon had relatively enhanced sediment community oxygen consumption rates and higher levels of phytopigments. particulate organic carbon and nucleic acids,...
Tipo: Text Palavras-chave: Zone abyssale; Canyon; Macrofaune; Métabolisme; Pigments végétaux; Deep-sea; Canyon; Macrofauna; Metabolism; Phytopigments.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00323/43370/42840.pdf
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Advances in improving mammalian cells metabolism for recombinant protein production Electron. J. Biotechnol.
Altamirano,Claudia; Berrios,Julio; Vergara,Mauricio; Becerra,Silvana.
Background: The production of recombinant proteins for therapeutic use represents a great impact on the biotechnology industry. In this context, established mammalian cell lines, especially CHO cells, have become a standard system for the production of such proteins. Their ability to properly configure and excrete proteins in functional form is an enormous advantage which should be contrasted with their inherent technological limitations. These cell systems exhibit a metabolic behaviour associated with elevated cell proliferation which involves a high consumption of glucose and glutamine, resulting in the rapid depletion of these nutrients in the medium and the accumulation of ammonium and lactate. Both phenomena contribute to the limitation of cell...
Tipo: Journal article Palavras-chave: Aerobic glycolysis; Cell engineering; CHO cells; Glutaminolysis; Metabolism.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300010
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Allocation du carbone et métabolisme azoté chez l'haptophyte Tisochrysis lutea ArchiMer
Garnier, Matthieu.
The aim of this thesis is to improve knowledge on mechanisms involved in the response to nitrogen limitation and in lipid accumulation in the microalgae haptophyte Tisochrysis lutea. The wild type strain and a lipid accumulating mutant strain were grown under different nitrogen limitation and starvation and analyzed by functional genomics. Four genes of high-affinity nitrate/nitrite transporter (Nrt2) were identified and characterized to reveal the mechanisms involved in mineral absorption in this species. Transcriptomes of both strains were sequenced and proteins affected by nitrogen starvation and differentially expressed between the two strains were identified. We so identified the functions regulated by nitrogen deficiency and potentially involved in...
Tipo: Text Palavras-chave: Microalgues; Métabolisme; Azote; Carbone; Lipides; Genomique fonctionelle; Protéomique; Transcriptomique; Microalgae; Metabolism; Nitrogen; Carbon; Lipids; Functional genomics; Proteomics; Transcriptomics.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00353/46378/46000.pdf
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Application of a micro-respirometric volumetric method to respiratory measurements of larvae of the Pacific oyster Crassostrea gigas ArchiMer
Goulletquer, Philippe; Wolowicz, Maciej; Latala, Adam; Brown, Craig; Cragg, Simon.
The application of a simple, fast and reliable volumetric microrespirometric method to assess respiration of bivalve larvae is discussed. As a model, C. gigas larvae of various sizes were used. Metabolic activity of veliger larvae was assessed by measuring respiratory rate for use in ecophysiological modelling. As an example of the application of this approach, additional measurements of veliger respiratory rates were carried out to assess the effect on larval metabolism of different concentrations of leachate from wood treated with chromated copper-arsenate (CCA). Veligers of Crassostrea gigas (length from 95 to 331 pm) were fed with a mixture of Isochrysis galbana) and Chaetoceros pumilum cells. Experiments were performed in a 20 degreesC constant...
Tipo: Text Palavras-chave: Crassostrea gigas; Leachate; CCA; Volumetric micro respirometry; Larvae; Metabolism; Respiratory rate.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-399.pdf
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Assimilação metabólica de nitrogênio em plântulas de seringueira cultivadas com nitrato ou amônio. Repositório Alice
SHAN, A. yu K. V.; OLIVEIRA, L. E. M. de; BONOME, L. T. da S.; MESQUITA, A. C..
O objetivo deste trabalho foi avaliar a assimilação metabólica de nitrogênio em plântulas de seringueira cultivadas na presença de nitrato ou de amônio, por meio da quantificação da atividade das enzimas redutase do nitrato (RN), glutamina sintetase (GS), glutamato sintase (GOGAT) e glutamato desidrogenase (GDH). Os seguintes tratamentos foram avaliados: fontes de N ? controle, sem aplicação de N; amônio a 8 mmol L‑1; e nitrato a 8 mmol L‑1 ? e tempos de exposição ? 0 e 27 dias ?, em arranjo fatorial 3x2, no delineamento inteiramente casualizado, com quatro repetições. A forma amoniacal promoveu maior assimilação metabólica de N, o que resultou em maior acúmulo de aminoácidos. A assimilação, tanto da forma nítrica quanto da amoniacal,...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Metabolismo nitrogenado; Plântula; Seringueira; Nitrato; Amonia; Hevea brasiliensis; Enzyme activity; Nitrogen; Metabolism; Nitrates.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/930334
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Associations between tissue fatty acid composition and physiological traits of performance and metabolism in the seabass (Dicentrarchus labrax) ArchiMer
Chatelier, Aurelien; Mckenzie, David; Prinet, Aurelie; Galois, Robert; Robin, Jean; Zambonino, Jose-luis; Claireaux, Guy.
Seabass were fed for 4 months with diets where the lipid was provided as either canola oil (CO), palm oil ( PO) or fish oil (FO), to generate diversity in their tissue fatty acid (FA) composition and investigate how this influenced major traits of exercise performance, cardiac performance and respiratory metabolism. In particular, based upon previous observations, we investigated the hypothesis that enriching the fish tissues with oleic and linoleic acids (OA, 18:1n-9 and LA, 18:2n-6, respectively) from the CO and PO diets would improve maximum exercise and cardiac performance, and increase aerobic metabolic scope. This proved to be the case; exercise respirometry on bass fitted with cardiac flow probes revealed that those fed CO and PO diets had a...
Tipo: Text Palavras-chave: Diet; Fatty acid; Cardiovascular performance; Metabolism; Swimming; Dicentrarchus labrax; Seabass.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1860.pdf
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Bioenergética del besugo Pagrus pagrus. OceanDocs
Aristizabal, E.O..
The utilization of the energy of food by red porgy (Pagrus pagrus Linnaeus, 1758) from the Argentine Sea (SW Atlantic) was studied. From the energetic point of view, it is proposed that the strategy of patitioning, utilization and storage of the energy of food, is an indicator of the organism response to the environmental conditions. The main objective was to know the proportion of the energy of food derived to the different components of the bioenergetics budget. In order to estimate the monthly energy investment in reproductive growth, chemical analysis of gonads from fishes caught by the fishery fleet and from laboratory studies were performed. Two indexes were used to calculate the energy investment in female ovaries: the proportion of net daily energy...
Tipo: Theses and Dissertations Palavras-chave: Fish culture; Bioenergetics; Eggs; Fish larvae; Food consumption; Energy budget; Metabolism; Oxygen consumption; Fish culture; Eggs; Food consumption; Metabolism; Fish larvae; Oxygen consumption; Http://aims.fao.org/aos/agrovoc/c_2918; Http://aims.fao.org/aos/agrovoc/c_2502; Http://aims.fao.org/aos/agrovoc/c_3016; Http://aims.fao.org/aos/agrovoc/c_4769; Http://aims.fao.org/aos/agrovoc/c_2924; Http://aims.fao.org/aos/agrovoc/c_5478.
Ano: 2003 URL: http://hdl.handle.net/1834/1466
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Biomass distribution and physiological capabilities of bacteria in the water column above a sea grass system. ArchiMer
Velimirov, B; Herndl, G; Kavka, G.
The distribution pattern of bacteria in the water column above and within a Posidonia oceanica bed near Ischia (off the Gulf of Naples) was investigated and correlated with DOC and POM data for autumn and winter. Data from samples of 5 stations along a transect from shallow to deep showed rather low bacterial densities (10 super(4).ml super(-1)) for both seasons. The number of attached bacteria was higher in late winter as compared to autumn when the leaves are shed from the stands. Except for occasional peaks DOC ranged between 1.5 and 4.5 mg C/1 while POM concentrations ranged form 4 to 14 mg AFDW.1 super(-1). Hydrolytic capabilities of isolated bacterial colonies showed that more carbohydrate substrates could be fermented in winter as compared to...
Tipo: Text Palavras-chave: Posidonia oceanica; Microorganisms; Physiology; Seasonal distribution; Algae; Bacteria; Particulate organic matter; Metabolism; Water column; Fermentation; Sea grass.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-954.pdf
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Biomass, metabolism and nutrition of zooplankton in the Gulf of Lions (NW Mediterranean) ArchiMer
Gaudy, R; Youssara, F; Diaz, F; Raimbault, P.
Zooplankton biomass was measured at 20 stations located along five coastal-offshore transects in the Gulf of Lions (NW Mediterranean) during spring 1998 and winter 1999, using two nets equipped with 200 and 80 Pm mesh. Biomass was twice as abundant in spring. It increased toward the coast and the west of the gulf and was positively correlated to chlorophyll, primary production and organic particulate matter and negatively to temperature and salinity. The enrichment effect of Rhone river input and the oligotrophic influence of the north Mediterranean current (NMC) were the main distribution factors of zooplankton biomass. Higher biomass was obtained in coastal stations with the 80 mum net, which sampled more efficiently young larval stages and nauplii....
Tipo: Text Palavras-chave: Zooplankton; Métabolisme; Cycle saisonnal; Golfe du Lion; Zooplankton; Metabolism; Seasonal cycle; Gulf of Lions.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00321/43262/42993.pdf
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Brazil nut oil in diets for breeder cocks Animal Sciences
Rufino, João Paulo Ferreira; Cruz, Frank George Guimarães; Melo, Ramon Duque; Feijó, Julmar da Costa; Melo, Lucas Duque; Costa, Ana Paula Guimarães Cruz; Bezerra, Natalia dos Santos.
 This study aimed to evaluate the inclusion of Brazil nut oil in diets for breeder cocks on performance, semen analyses, biochemical serum profile and testicular morphology. Forty-two Rhode Island Red breeder cocks with 40 weeks of age were used. The experimental design was completely randomized consisting of seven levels of Brazil nut oil (0; 0.30; 0.60; 0.90; 1.20; 1.50 and 1.80%) in the diets. Data collected were evaluated by polynomial regression. Differences (p < 0.05) were detected in feed conversion, semen volume, motility, vigor, pH, swirling and concentration, in which the inclusion of Brazil nut oil in the diets improved the reproductive performance. Differences (p < 0.05) were also detected in the biochemical serum profile (triglycerides,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Additive; Fatty acids; Metabolism; Reproductive performance..
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/37472
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Cetose e infiltração gordurosa no fígado em vacas leiteiras. Infoteca-e
AROEIRA, L. J. M..
Alta demanda de energia e consumo inadequado fazem com que o animal entre num balanço energético negativo, mobilizando suas reservas de gorduras, predispondo-o à esteatose hepática e à cetose. Os sintomas mais característicos da doença, além do odor de acetona no hálito e na urina, são a perda do apetite, particularmente por concentrados, a diminuição da produção e a rápida perda da condição corporal. O documento foi escrito com o objetivo de tentar esclarr er os produtores, extensionistas, nutricionistas e estudantes sobre as causas, os mecanismos metabólicos, as prevenções e os tratamentos da cetose e da esteatose hepática. Essas doenças são ainda pouco comuns no Brasil, mas podem ocorrer com maior freqüência em rebanhos mais produtivos. Trata-se de uma...
Tipo: Documentos (INFOTECA-E) Palavras-chave: Bovinos de leite; Metabolismo; Doencas; Esteatose; Dairy cattle; Metabolism; Diseases.
Ano: 1998 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/593345
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Chitin/toxicant interactions and microbial degradation within estuarine ecosystems. ArchiMer
Portier, R; Meyers, S.
The environmental effect and fate of several agricultural and industrial source toxicants were evaluated in controlled laboratory microcosm systems in the presence of the aminopolysaccharide polymer, chitin. Toxicants examined included methyl parathion, phenol, pentachlorophenol, 2,4 dichlorophenol, 1,2 dichloroethane and Kepone. Microcosm operating parameters, including pH/Eh, salinity, temperature, flow rate and sediment/water interface conditions, were based on comparable in situ measurements. Fate analysis predictions were based on mineralization of the parent toxicant compound as measured by chromatographic analysis or super(14)CO sub(2) expiration. Chitin, amended to continuous flow microcosms, promoted either cometabolic or cooxidative...
Tipo: Text Palavras-chave: Controlled conditions; Chemical pollutants; Industrial wastes; Pesticides; Metabolism; Food webs; Biodegradation; Ecosystems; Microorganisms; Environmental effects; Brackishwater pollution; Estuaries; Toxicants; Chitin.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1011.pdf
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Comparative analysis of oxygen consumption rates between cupped oyster spat of Crassostrea gigas of French, Japanese, Spanish and Taiwanese origins ArchiMer
Goulletquer, Philippe; Wolowicz, Maciej; Latala, Adam; Geairon, Philippe; Huvet, Arnaud; Boudry, Pierre.
Respiration rates of various geographical strains of Crassostrea gigas were compared to assess the respiratory expenditure as a physiological indicator of catabolism (cost of maintenance). Parental oysters, sampled in France (Marennes-Oleron), Japan (Hiroshima), Taiwan (Tunkang) and Spain (Cadix), were differentiated by both their geographic origin and by mitochondrial DNA markers, allowing the distinction between the two closely related taxa Crassostrea gigas and Crassostrea angulata. After reproduction of these parental oysters, respiration rates of spat of each strain, reared under common controlled conditions, were individually estimated at 20 degrees C by using a volumetric microrespirometer. Our results demonstrated that physiological variability...
Tipo: Text Palavras-chave: Pacific Ocean; NE Atlantic; Oyster strains; Hybrids; Crassostrea angulata; Crassostrea gigas; Metabolism; Respiration rates; Océan Pacifique; Atlantique NE; Souches génétiques; Hybrides; Crassostrea angulata; Crassostrea gigas; Métabolisme; Taux de respiration.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-859.pdf
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Concentrações séricas hormonais em vacas azebuadas submetidas à baixa e alta ingestão alimentar. Repositório Alice
MARTINS, A.C.; MOLLO, M.R.; BASTOS, M.R.; GUARDIEIRO, M.M.; SARTORI, R..
O objetivo deste trabalho foi avaliar a influência da ingestão alimentar nas concentrações séricas de hormônios reprodutivos e metabólicos em vacas azebuadas. Dezoito vacas foram divididas em dois grupos: 170% (alta ingestão = A) e 66% (baixa ingestão = B) da dieta de manutenção. Com 21 dias nas dietas experimentais, as vacas tiveram o estro sincronizado. Posteriormente, os ovários foram avaliados por ultra-sonografia transretal e sangue foi coletado diariamente até o dia 7 do ciclo (ovulação = dia 1). Na análise estatística, utilizou-se o teste t. As vacas ganharam 1,1 kg por dia no grupo A e perderam 1,5 kg por dia de PV no grupo B. Apesar de não ter havido diferença entre os grupos no diâmetro máximo do folículo ovulatório, o grupo A apresentou pico...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bos indicus; Corpo lúteo; Esteróides; Folículo; Metabolismo; Nutrição; Corpus luteum; Steroids; Follicle; Metabolism; Nutrition.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/123643
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Consumo de oxígeno, crecimiento y utilización del alimento durante el desarrollo larval del besugo Pagrus pagrus (Linné, 1758)(Pisces:Sparidae). OceanDocs
Aristizabal, E.O..
Knowledge of the life history of fish larvae is of practical importance to understand variations in the survival rate not only for cultivated fish but also for fishery biology studies. With the aim of recognizing critical changes during the first days of life of red porgy larvae, the oxygen consumption, growth, and food utilization were studied. After hatching, the larvae were transferred to two 400 L fiberglass tanks with microalgae (Nannochloropsis oculata)and enriched rotifers (Brachionus plicatilis)as initial food. During the incubation and larval rearing, water temperature was kept at 18.0 ± 0.5ºC and salinity ranged from 33.0 to 34.0 psu. Results indicate a decrease in larval growth between day 3 and 4 after hatching, when larvae opened their mouths,...
Tipo: Journal Contribution Palavras-chave: Fish culture; Marine fish; Fish larvae; Metabolism; Oxygen consumption; Fish culture; Metabolism; Fish larvae; Oxygen consumption; Http://aims.fao.org/aos/agrovoc/c_2918; Http://aims.fao.org/aos/agrovoc/c_4769; Http://aims.fao.org/aos/agrovoc/c_2924; Http://aims.fao.org/aos/agrovoc/c_5478.
Ano: 2006 URL: http://hdl.handle.net/1834/1557
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Cultivating the uncultured: limits, advances and future challenges ArchiMer
Alain, Karine; Querellou, Joel.
Since the invention of the Petri dish, there have been continuous efforts to improve efficiency in microbial cultivation. These efforts were devoted to the attainment for diverse growth conditions, simulation of in situ conditions and achievement of high-throughput rates. As a result, prokaryotes catalysing novel redox reactions as well as representatives of abundant, but not-yet cultured taxa, were isolated. Significant insights into microbial physiology have been made by studying the small number of prokaryotes already cultured. However, despite these numerous breakthroughs, microbial cultivation is still a low-throughput process. The main hindrance to cultivation is likely due to the prevailing lack of knowledge on targeted species. In this review, we...
Tipo: Text Palavras-chave: High throughput cultivation; Cell cell communication; Microbial community; Metabolism; Isolation; Culture.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6820.pdf
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Cyanogenesis and plant resistance to microbial attack. Repositório Alice
LIEBEREI, R.; GIESEMANN, A.; SELMAR, D.; BIEHL, B.; MEVENKAMP, G.; JUNQUEIRA, N.T.V..
Cyanogenic glucosides are metabolized during the development of Hevea brasiliensis seedlings. Changes in the content of cyanogen were analyzed in other Hevea species and varieties. Whereas the content of linamarin stored in the endosperm decreased in all Hevea species, the amount of cianogenic glucosides in the developing seedling varied greatly.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Seringueira; Hevea; Especie; Cianogenese; Linamarine; Metabolismo; Rubber tree; Cyanogenic glycosides; Linamarin; Metabolism.
Ano: 1987 URL: http://www.alice.cnptia.embrapa.br/handle/doc/667356
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Daytime soybean transcriptome fluctuations during water deficit stress. Repositório Alice
RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; LOBO, F. P.; HARMON, F. G.; NEPOMUCENO, A. L..
bitstream/item/166586/1/Nepomuceno-BMC-daytime.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Soja; Seca; Metabolismo vegetal; Abiotic stress; Metabolism; Drought; Soybeans.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1079491
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Defasagem entre as produtividades real e potencial do algodoeiro herbáceo: limitações morfológicas, fisiológicas e ambientais. Infoteca-e
BELTRÃO, N. E. de M.; AZEVEDO, D. M. P. de..
Fatores limitantes relacionados com a morfologia, a organografia e arquiteture do algodoeiro herbaceo; fatores limitantes relacionados com -a fisiologia e a bioquimica da planta do algodoeiro herbaceo; fatores limitantes relacionados com as condicoes do ambiente; consideracoes holoticas envolvendo fatores da planta e do ambiente.
Tipo: Livro técnico (INFOTECA-E) Palavras-chave: Algodao; Herbaceo; Fisiologia vegetal; Producao; Taxas de Crescimento; Cultura; Fotossintese; Respiracao; Fotorrespiracao; Trofofilos; Dossel; Balanco; Assimilado; Bioquimica; Metabolismo; Limitacao incontrolavel; Crescimento; Desenvolvimento; Gossypium hirsutum; Brasil; Paraiba; Annual cotton; Physiology; Production; Growth rate; Photosynthesis; Reespiration; Photorespiration; Assimilation; Leaves; Crop; Canopy; Levll; Metabolism; Biochemistry; Not controllable; Limitation; Growth; Deelopment.
Ano: 1993 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/264934
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Diet-induced developmental plasticity in life histories and energy metabolism in a beetle RChHN
URREJOLA,SERGIO; NESPOLO,ROBERTO; LARDIES,MARCO A.
Adaptive phenotypic plasticity, has been recognized as an important strategy by which organisms maximize fitness in variable environments, which vary through development. A disassociation among stages should represent a null effect of the environment experienced during early ontogeny in the expression of adult traits. Food quality greatly influences survival, development and reproduction in many arthropod herbivores. We examined the effects of diet protein in physiological and life-history traits in the yellow mealworm beetle Tenebrio molitor through ontogeny. We established four experimental treatments: Low Protein (LP), Low Protein Control (LPC), High Protein (HP), and High Protein Control (HPC) with recently eclosioned larvae each. Individuals were...
Tipo: Journal article Palavras-chave: CO2 production; Complex life-cycle; Food quality; Metabolism; Ontogeny.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2011000400005
Registros recuperados: 112
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