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Registros recuperados: 34
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A Temporal diversity analysis of brazilian begomoviruses in tomato reveals a decrease in species Richness between 2003 and 2016. Repositório Alice
SOUZA, T. A.; SILVA, J. M. F.; NAGATA, T.; MARTINS, T. P.; NAKASU, E. Y. T.; INOUE-NAGATA, A. K..
In this study, we estimated genetic diversity changes in begomoviruses that infect tomato plants in an important tomato-growing region in Central Brazil through ~ 14 years.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: ToMoLCV; ToSRV; Bemisia Tabaci; Begomovirus; Metagenomics; Tomatoes; Genome; Solanum lycopersicum.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1124206
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Comparative study of poultry gut and litter microbiota through a metagenomic approach. Repositório Alice
PANDOLFI, J. R. C.; SILVA, V. S.; KRAMER, B.; PEIXOTO, J. de O.; CANTAO, M. E.; LOYOLA, W.; COLDEBELLA, A.; RECH, D. V.; TREVISOL, I. M.; ESTEVES, P. A.; JAENISCH, F. R. F.; KLEIN, T. A. P.; VAZ, C. S. L.; CARON, L.; SCHIOCHET, M. F.; LOPES, L. dos S.; IBELLI, A. M. G.; TESSMANN, A. L.; MORES, N.; BRENTANO, L.; SANTOS, F. B. de O.; LEDUR, M. C.; IANISKI, F.; SILVA, M. L. B. da.
2012
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Microbiologia; Produção animal; Metagenomics; Broiler chickens; Poultry production.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/953157
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Composition and functionality of the wild and cultivated common bean rhizosphere microbiome. Repositório Alice
FLORES, S. W. S.; CHIARAMONTE, J. B.; ROSSMANN, M.; MENDES, R..
Abstract: Plants rely on their rhizosphere microbiome for specific functions, such as, nutrient acquisition and protection against diseases. The domestication and subsequent plant breeding neglected the important role of the rhizosphere microbiome on plant performance. Here, we tested the hypothesis that ancestor materials have higher ability to host beneficial microorganisms in the rhizosphere when compared to modern cultivars. For this, we assessed the composition and functionality of the rhizosphere microbiome associated with a wild (Wild Mex) and with a cultivated (IAC Alvorada) common bean grown in highly biodiverse soil (Amazonian Dark Earth). Antagonistic bacteria were isolated from common bean rhizosphere and total rhizosphere DNA was extracted for...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Wild common bean; Metagenome; Rhizobacteria; Metagenomics; Microbial communities.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1036667
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Construction and characterization of a metagenomic DNA library from the rhizosphere of wheat (Triticum aestivum) Phyton
Hernández-León,R; Martínez-Trujillo,M; Valencia-Cantero,E; Santoyo,G.
Rhizospheric soil of wheat plants contains a high diversity of microorganisms, and therefore, comprises a large reservoir for discovering genes with diverse agro-biotechnological applications. In this work, we constructed an E. coli metagenomic library based on bacterial artificial chromosome (BAC) clones with large genomic inserts from metagenomic DNA from the rhizosphere of wheat plants. The average of the DNA cloned segments varies from 5 to 80 kb, with an average size of 38 kb. Random clones were end-sequenced and homology results showed that the clonation of metagenomic DNA codes mainly for metabolic and catalytic functions (40%), including amidohydrolase, hydrolase, peptidase, serine protease, endonuclease and exonuclease. Another interesting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metagenomics; Rhizosphere; Wheat.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100018
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Co-occurrence patterns of litter decomposing communities in mangroves indicate a robust community resistant to disturbances. Repositório Alice
TAKETANI, R. G.; MOITINHO, M. A.; MAUCHLINE, T.; MELO, I. S. de.
Abstract: Background. Mangroves are important coastal ecosystems known for high photosynthetic productivity and the ability to support marine food chains through supply of dissolved carbon or particular organic matter. Most of the carbon found in mangroves is produced by its vegetation and is decomposed in root associated sediment. This process involves a tight interaction between microbial populations, litter chemical composition, and environmental parameters. Here, we study the complex interactions found during litter decomposition in mangroves by applying network analysis to metagenomic data.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Litter decomposition; Mangue; Planta; Decomposição; Bactéria; Mangrove forests; Metagenomics.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1098904
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Dinâmica do microbioma ruminal de ovinos Ovis aries e sua relação com a degradação da biomassa. Repositório Alice
ROMAGNOLI, E. M..
Considerando a dieta como um fator modulador do microbioma ruminal, neste trabalho objetivou-se investigar o impacto do bagaço da cana-de-açúcar sobre a composição e funcionalidade das espécies microbianas residentes no rúmen de carneiros (Ovis aries). Foram utilizados seis animais machos fistulados de O. aries, dos quais três foram alimentados com uma dieta composta por 70% de volumoso e 30% de concentrado (tratamento controle) e outros três animais alimentados com uma dieta similar a anterior, mas com 14% do volumoso substituído por bagaço de cana-de-açúcar (tratamento bagaço). O conteúdo ruminal (líquido e fibra) foram amostrados quinzenalmente durante 60 dias. A partir dessas amostras foram acessadas a estrutura e a composição da comunidade microbiana...
Tipo: Tese/dissertação (ALICE) Palavras-chave: Rúmen; Metagenômica; Metatranscriptômica 165 rRNA; Bagaço; Cana-de-açúcar; Metagenomics; Sugarcane bagasse.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1065429
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Effect of nickel in the degradation of oil in soils contaminated with petroleum and nickel. Repositório Alice
TAKETANI, N. F.; TAKETANI, R. G.; LEITE, S. G. F.; MELO, I. S. de; LIMA-RIZZO, A. C.; ANDREOTE, F. D.; CUNHA, C. D. da.
Abstract: The pollution originated from the petrochemical industry is currently one of the main environmental concerns. Most of this is due to the high volume of toxic hydrocarbons that are produced and transported around the globe. However, this industry is also associated with toxic metals that are minor components of petroleum and used during refining processes. Here we have evaluated the impact of nickel in the biodegradation of crude oil in natural soils. We have assessed bacterial community profiles in these samples using ion torrent 16S rRNA gene sequencing and Real-Time PCR quantification and shotgun metagenome sequencing. We have found that the contamination with oil and nickel reduced the bacterial abundance compared with only soil. There was...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Metagenome; Metal contamination; Hydrocarbon remediation; Contaminação; Poluição do Solo; Petróleo; Níquel; Bactéria; Metagenomics; Soil pollution; Petroleum; Actinobacteria; Decontamination.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125486
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Functional Genes of Microorganisms, Comprehending the Dynamics of Agricultural Ecosystems BABT
Reali,Catiusca; Pilz Júnior,Harry Luiz; Meile,Jean Christophe; Galindo,Sabine Shorr; Fiuza,Lidia Mariana.
ABSTRACT The microbial composition of different types,in ecosystems (including agro-ecosystems), has been investigated in a rapidly growing number of studies in the past few years. The importance of microorganisms, regarding the maintenance and stability of nutrients in agroecosystems, is a key to maintain the sustainability of a crop. Molecular tools to study microbial communities are possible through many methods such as RISA, DGGE, TGGE, clone libraries, T-RFLP, RAPD, SSCP and more recently NGS (Next-Generation Sequencing). DGGE is widely employed to characterize the diversity and the community dynamics of microorganisms in the environment, making possible to find out specific groups through functional genes, allowing access to data that cannot be...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agroecosystems; Functional genes; Bacteria; DGGE; Metagenomics; Microbial ecology.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100201
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Functional screening of a Caatinga goat (Capra hircus) rumen metagenomic library reveals a novel GH3 beta-xylosidase. Repositório Alice
SOUTO, B. de M.; ARAÚJO, A. C. B. de; HAMANN, P. R. V.; BASTOS, A. de R.; CUNHA, I. de S.; PEIXOTO, J.; KRUGER, R. H.; NORONHA, E. F.; QUIRINO, B. F..
Functional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized. Recombinant BGL11 was obtained and kinetically characterized. Substrate specificity of the purified protein was assessed using seven synthetic aryl substrates. Activity towards nitrophenyl-beta-D-glucopyranoside (pNPG), 4-nitrophenyl- beta -D-xylopyranoside (pNPX) and 4-nitrophenyl- beta -D-cellobioside (pNPC) suggested that BGL11 is a multifunctional enzyme with beta-glucosidase, beta-xylosidase,...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Enzima; Clonagem; Programa de Computador; Peptídeo; Metagenomics; Enzymes; Cloning (animals); Signal peptide; Prevotella; Computer software; Screening.
Ano: 2021 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1129624
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Genome mining of the rhizophere bacterium Pseudomonas sp. SH-C52. Repositório Alice
VOORT, M. V. D.; SCHMIDT, Y.; WATROUS, J.; MENDES, R.; GROSS, H.; DORRESTEIN, P.C.; RAAIJMAKERS, J. M..
2012
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Pseudomonas sp. SH-C52; Rhizophere; Genome; Metagenomics.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/956388
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Genomic ecology of Marine Group II, the most common marine planktonic Archaea across the surface ocean ArchiMer
Pereira, Olivier; Hochart, Corentin; Auguet, Jean-christophe; Debroas, Didier; Galand, Pierre E..
Planktonic Archaea have been detected in all the world's oceans and are found from surface waters to the deep sea. The two most common Archaea phyla are Thaumarchaeota and Euryarchaeota. Euryarchaeota are generally more common in surface waters, but very little is known about their ecology and their potential metabolisms. In this study, we explore the genomic ecology of the Marine Group II (MGII), the main marine planktonic Euryarchaeota, and test if it is composed of different ecologically relevant units. We re‐analyzed Tara Oceans metagenomes from the photic layer and the deep ocean by annotating sequences against a custom MGII database and by mapping gene co‐occurrences. Our data provide a global view of the distribution of Euryarchaeota, and more...
Tipo: Text Palavras-chave: 16S rRNA; Euryarchaeota; Global ocean; Metagenomics; Poseidoniales; Proteorhodopsin.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00505/61676/65607.pdf
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Holobionts and their hologenomes: Evolution with mixed modes of inheritance Genet. Mol. Biol.
Haag,Karen Luisa.
Abstract Symbioses are ubiquitous and have played an influential role in the evolution of life on Earth. Genomic studies are now revealing a huge diversity of associations among hosts and their microbiotas, allowing us to characterize their complex ecological and evolutionary dynamics. The different transmission modes and the asynchronous cell proliferation of the numerous symbionts associated with one host generate a genomic conflict ought to be solved. Two disputing views have been used to model and predict the outcome of such conflicts. The traditional view is based on community ecology, and considers that selection at the level of individuals is sufficient to explain longstanding associations among species. A new perspective considers that the host and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genomics; Metagenomics; Microbiota; Symbiosis; Holobiont.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200189
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Metagenoma da rizosfera de mandacaru na Caatinga. Repositório Alice
FERREIRA, C.; TAKETANI, R. G.; SILVA, J. L. da; GAVA, C. A. T.; MELO, I. S. de; MENDES, R..
2012
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Caatinga; Metagenoma; Ion Torrent; Rizosfera; Mandacaru; Metagenomics.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/956386
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Metagenomic analysis of the rhizosphere microbiome of the common bean resistant to Fusarium oxysporum. Repositório Alice
MENDES, L. W.; MENDES, R.; TSAI, S. M..
The rhizosphere microbiome plays a key role in the functioning of the host plant, influencing its physiology and development. It has been suggested that plants use mechanisms present in the rhizosphere microbiome to fend off infections, such as fungal diseases. This work aimed to assess the microbial community inhabiting the common bean rhizosphere in order to identify potential groups related to the suppression of the soil-borne pathogen Fusarium oxysporum. Therefore, using shotgun metagenomic sequencing (Illumina Miseq), we investigated the phylogenetic and potential functional diversity of microbial communities colonizing the rhizosphere of four cultivars of common bean with different levels of resistance to the fungus, ranging from high susceptibility...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Metagenômica; Feijão; Fusarium oxysporum; Metagenomics.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1036658
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Metagenomic and metatranscriptomic analysis of the microbial community structure and metabolic potential of fermented soybean in Yunnan Province Ciênc. Tecnol. Aliment.
LIU,Xiao-Feng; LIU,Chen-Jian; ZENG,Xue-Qin; ZHANG,Hai-Yan; LUO,Yi-Yong; LI,Xiao-Ran.
Abstract Traditional fermented soybean contains a variety of microflora. To obtain a comprehensive and accurate understanding of the microbial community structure and metabolic potential of fermented soybean, comparative metagenomics and metatranscriptomics were performed on a sample of fermented soybean in Yunnan Province. Metagenomic DNA and metatranscriptomic RNA were sequenced using Illumina HiseqTM2500, which yielded a total of 92,192,276 reads, with an average read length of 150 bp and 38,798,262 paired-end sequences, with an average length of 151 bp. The results show that Providencia stuartii was the most abundant species and the genes of carbohydrates (2296, 13.30%), protein metabolism (1530, 8.86%), and amino acids and amino acid derivatives...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fermented soybean; Metagenomics; Metatranscriptomics; Microbial community.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019005000301
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Metagenomic and metatranscriptomic analysis of the microbial community structure and metabolic potential of fermented soybean in Yunnan Province Ciênc. Tecnol. Aliment.
LIU,Xiao-Feng; LIU,Chen-Jian; ZENG,Xue-Qin; ZHANG,Hai-Yan; LUO,Yi-Yong; LI,Xiao-Ran.
Abstract Traditional fermented soybean contains a variety of microflora. To obtain a comprehensive and accurate understanding of the microbial community structure and metabolic potential of fermented soybean, comparative metagenomics and metatranscriptomics were performed on a sample of fermented soybean in Yunnan Province. Metagenomic DNA and metatranscriptomic RNA were sequenced using Illumina HiseqTM2500, which yielded a total of 92,192,276 reads, with an average read length of 150 bp and 38,798,262 paired-end sequences, with an average length of 151 bp. The results show that Providencia stuartii was the most abundant species and the genes of carbohydrates (2296, 13.30%), protein metabolism (1530, 8.86%), and amino acids and amino acid derivatives...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fermented soybean; Metagenomics; Metatranscriptomics; Microbial community.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000100018
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Metagenomic sequencing of environmental DNA reveals marine faunal assemblages from the West Antarctic Peninsula ArchiMer
Cowart, Dominique; Murphy, Katherine R.; Cheng, C. -h. Christina.
The West Antarctic Peninsula (WAP) is the fastest warming region in Antarctica where climate impact on the cold-adapted marine ecosystem is already visible. To monitor faunal changes in remote vast bodies of Antarctic waters, efficient and informative tools are essential. High-throughput sequencing of environmental DNA (eDNA) has emerged as one such tool for monitoring biodiversity and ecosystems, as it increases detection sensitivity of taxa, and sampling is often simpler and less costly than traditional collection methods. We collected water samples from four WAP shallow (≤ 300 m) shelf regions, recovered the eDNA therein, and performed metagenomic shotgun sequencing and analyses to determine the effectiveness of this method to assess marine benthic...
Tipo: Text Palavras-chave: EDNA; Antarctica; Metagenomics; King crabs; Benthic invertebrates.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00413/52490/53278.pdf
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Metagenomics and metatranscriptomics of the rhizosphere microbiome: understanding the interplay between the good, the bad and the ugly. Repositório Alice
RAAIJMAKERS, J. M.; CHAPELLE-PINEAU, E.; BAKKER, P. A. H. M.; MENDES, R..
2012
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Rhizosphere; Metagenoma; Metagenomics.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/956390
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Metagenomics of sheep rumen microbiome under two diet. Repositório Alice
LOPES, L. D; SILVA, L. R. F, da; ROMAGNOLI, E. M; FERREIRA, C.; TAKETANI, R. G.; ABDALLA, A. L.; LOUVANDINI, H.; MENDES, R..
2012
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sheep rumen microbiome; Metagenomics.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/956409
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Metagenomics of the rhizosphere microbiome: understanding the interplay between the good, the bad and the ugly. Repositório Alice
RAAIJMAKERS, J. M.; MENDES, R..
2012
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Rhizosphere microbiome; Metagenomics.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/956403
Registros recuperados: 34
Primeira ... 12 ... Última
 

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