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Registros recuperados: 107
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Algicidal effects of aqueous leaf extracts of neem (Azadirachta indica) on Scenedesmus quadricauda (Turp.) de Brébission Acta Botanica
Chia,Mathias Ahii; Akinsanmi,Jamiu Taiwo; Tanimu,Yahuza; Ladan,Zakari.
The application of synthetic algaecides for the control of algae produces by-products that are sometimes toxic to the environment. There is a need for natural and cheap alternatives to synthetic algaecides. In the present study, we investigated the potential of aqueous crude extract of Azadirachta indica to inhibit the growth of Scenedesmus quadricauda. Phytochemical screening of the extract revealed the presence of groups of bioactive compounds that are capable of inhibiting microalgal growth. Chlorophyll a concentration, dry weight production and cell density of microalga decreased with increasing crude extract concentration. After three days of exposure, the 1000 mg/L extract concentration resulted in complete growth inhibition and cell lysis....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Algaecide; Growth inhibition; Microalgae; Neem; Oxidative stress; Phenotypic plasticity.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016000100001
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Allocation du carbone et métabolisme azoté chez l'haptophyte Tisochrysis lutea ArchiMer
Garnier, Matthieu.
The aim of this thesis is to improve knowledge on mechanisms involved in the response to nitrogen limitation and in lipid accumulation in the microalgae haptophyte Tisochrysis lutea. The wild type strain and a lipid accumulating mutant strain were grown under different nitrogen limitation and starvation and analyzed by functional genomics. Four genes of high-affinity nitrate/nitrite transporter (Nrt2) were identified and characterized to reveal the mechanisms involved in mineral absorption in this species. Transcriptomes of both strains were sequenced and proteins affected by nitrogen starvation and differentially expressed between the two strains were identified. We so identified the functions regulated by nitrogen deficiency and potentially involved in...
Tipo: Text Palavras-chave: Microalgues; Métabolisme; Azote; Carbone; Lipides; Genomique fonctionelle; Protéomique; Transcriptomique; Microalgae; Metabolism; Nitrogen; Carbon; Lipids; Functional genomics; Proteomics; Transcriptomics.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00353/46378/46000.pdf
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Antimelanoma activity of Heterocapsa triquetra pigments ArchiMer
Haguet, Quentin; Bonnet, Antoine; Berard, Jean-baptiste; Goldberg, Joelle; Joguet, Nicolas; Fleury, Audrey; Thiery, Valerie; Picot, Laurent.
Heterocapsa triquetra (Ht), a non-toxic dinophyte, was selected to investigate the presence of pigments inhibiting melanoma cells growth. We developed an innovative microwave-assisted extraction process (MAE), determined the Ht pigment composition using Reverse Phase-High Performance Liquid Chromatography (HPLC) and high-resolution Ultra Performance Liquid Chromatography-Mass Spectrometry E (UPLC-MSE) analysis and assessed their antiproliferative activity in human invasive melanoma cells (A2058) grown in vitro. MAE allowed a fast and efficient extraction of Ht pigments as compared with conventional processes (soaking, sonication). Seventeen pigments and derivatives were unequivocally identified by UPLC-MSᴱ in the Ht extract, among which two were supposed...
Tipo: Text Palavras-chave: Dinophyte; Heterocapsa; Melanoma; Microalgae; Microwave assisted extraction; Pigment.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00390/50127/50806.pdf
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Antimicrobial Compounds from Eukaryotic Microalgae against Human Pathogens and Diseases in Aquaculture ArchiMer
Falaise, Charlotte; Francois, Cyrille; Travers, Marie-agnes; Morga, Benjamin; Haure, Joel; Tremblay, Rejean; Turcotte, Francois; Pasetto, Pamela; Gastineau, Romain; Hardivillier, Yann; Leignel, Vincent; Mouget, Jean-luc.
The search for novel compounds of marine origin has increased in the last decades for their application in various areas such as pharmaceutical, human or animal nutrition, cosmetics or bioenergy. In this context of blue technology development, microalgae are of particular interest due to their immense biodiversity and their relatively simple growth needs. In this review, we discuss about the promising use of microalgae and microalgal compounds as sources of natural antibiotics against human pathogens but also about their potential to limit microbial infections in aquaculture. An alternative to conventional antibiotics is needed as the microbial resistance to these drugs is increasing in humans and animals. Furthermore, using natural antibiotics for...
Tipo: Text Palavras-chave: Biological activity; Antimicrobial; Antibacterial; Antifungal; Antiviral; Biological activity; Haslea; Microalgae; Sustainable aquaculture.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00349/46052/45719.pdf
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Antiproliferative Activity of Cyanophora paradoxa Pigments in Melanoma, Breast and Lung Cancer Cells ArchiMer
Baudelet, Paul-hubert; Gagez, Anne-laure; Berard, Jean-baptiste; Juin, Camille; Bridiau, Nicolas; Kaas, Raymond; Thiery, Valerie; Cadoret, Jean-paul; Picot, Laurent.
The glaucophyte Cyanophora paradoxa (Cp) was chemically investigated to identify pigments efficiently inhibiting malignant melanoma, mammary carcinoma and lung adenocarcinoma cells growth. Cp water and ethanol extracts significantly inhibited the growth of the three cancer cell lines in vitro, at 100 mu g.mL(-1). Flash chromatography of the Cp ethanol extract, devoid of c-phycocyanin and allophycocyanin, enabled the collection of eight fractions, four of which strongly inhibited cancer cells growth at 100 mu g.mL(-1). Particularly, two fractions inhibited more than 90% of the melanoma cells growth, one inducing apoptosis in the three cancer cells lines. The detailed analysis of Cp pigment composition resulted in the discrimination of 17 molecules, ten of...
Tipo: Text Palavras-chave: Pigments; Microalgae; Cyanophora paradoxa; Melanoma; Cancer; Apoptosis; Zeaxanthin; Cryptoxanthin.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00177/28818/27289.pdf
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Antiproliferative Activity of Violaxanthin Isolated from Bioguided Fractionation of Dunaliella tertiolecta Extracts ArchiMer
Pasquet, Virginie; Morisset, Perrine; Ihammouine, Said; Chepied, Amandine; Aumailley, Lucie; Berard, Jean-baptiste; Serive, Benoit; Kaas, Raymond; Lanneluc, Isabelle; Thiery, Valerie; Lafferriere, Mathieu; Piot, Jean-marie; Patrice, Thierry; Cadoret, Jean-paul; Picot, Laurent.
Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose- dependent growth inhibition in continuous and discontinuous treatments, at...
Tipo: Text Palavras-chave: Pigments; Microalgae; Dunaliella; Violaxanthin; Carotenoid; Cancer; Apoptosis.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00040/15142/12575.pdf
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Apparent metabolizable energy from microalgae spirulina maxima for broiler chickens. Repositório Alice
TAVERNARI, F. de C.; ROZA, L.; SUREK, D.; SILVA, M. L. B. da.
2016
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Energy; Brollers; Microalgae; Spirulina maxima.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1045619
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Avaliação do uso de nanopartículas de quitosana na coagulação/F-loculação de microalgas crescidas em efluente suinícola. Repositório Alice
FONSECA, F. N. da; MICHELON, W.; MEZZARI, M. P.; SILVA, M. L. B. da.
O presente trabalho investigou a eficiência de uma solução de quitosana nanoparticulada na coagulação/ floculação de biomassa de microalgas Chlorella vulgaris, cultivada em efluente suinícola laboratório e em fotobioreatores de escala laboratorial. O efeito da quitosana nanoparticulada foi comparado ao uso da quitosana na forma pura utilizando testes de jarros. Entre as concentrações testadas (20, 30, 40 e 60 mg L-1), a nanoquitosana na dose de 40 mg L-1 apresentou a maior recuperação de biomassa (94%), enquanto a solução de quitosana pura (30 mg L-1) foi de 87%. Além disso, a nanoquitosana apresentou maior capacidade total de recuperação. Os resultados sugerem que as nanoparticulas de quitosana podem ser uma alternativa interessante de polímero orgânico...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Nanotecnologia; Dejeto; Suíno; Microalga; Nanotechnology; Pig manure; Microalgae.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1013962
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Bacterial community structure of the marine diatom Haslea ostrearia ArchiMer
Lepinay, Alexandra; Capiaux, Herve; Turpin, Vincent; Mondeguer, Florence; Lebeau, Thierry.
Haslea ostrearia produces a water-soluble, blue-green pigment, called marennine, with proven economic benefits (as a bioactive compound used to green oysters, which improves their market value). Incomplete knowledge of the ecological features of this marine diatom complicates its cultivation. More specifically, the ecology of bacteria surrounding H. ostrearia in ponds is what remains unknown. The structure of this bacterial community was previously analyzed by means of PCR-TTGE before and after isolating H. ostrearia cells recovered from 4 localities in order to distinguish the relative parts of the biotope and biocenose and to describe the temporal dynamics of the bacterial community structure at two time scales (2 weeks vs. 9 months). The bacterial...
Tipo: Text Palavras-chave: Biofilm; Ecology; Metabolic fingerprinting; Microalgae; Phycosphere; TTGE.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00326/43737/43344.pdf
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Bioethanol from Microalgal Biomass: A Promising Approach in Biorefinery BABT
Silva,Carlos Eduardo de Farias; Bertucco,Alberto.
Abstract The development of new technologies which increase the production of biofuel without directly compete with food production is required. Microalgal biomass has recently been in the highlight. The role of this biomass is here discussed within the concept of biorefinery and industrial sustainability of bioethanol production. The process of cultivation in order to accumulate around 50% of carbohydrates in the biomass (dry weight) and the importance of water and nutrient recycling are reviewed. Saccharification of biomass using enzymes or acids and alternative processes such as hydrothermal liquefaction and flash hydrolysis are addressed. Since the main monosaccharide in microalgal biomass is glucose, high rates of hydrolysis and fermentation were,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethanol; Microalgae; Biofuel; Hydrolysis; Nutrient recycle.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100602
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Biogas production from microalga biomass. Repositório Alice
PERAZZOLI, S.; STEINMETZ, R. L. R.; MEZZARI, M. P.; NUNES, E. O.; SILVA, M. L. B. da.
2013
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Tratamento de efluentes; Biogás; Microalga; Suinocultura; Wastewater treatment; Pig farming; Microalgae; Methane.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/970050
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Broiler chicken ileal apparent digestibility of calcium and phosphorus from microalgae spirulina maxima. Repositório Alice
TAVERNARI, F. de C.; SUREK, D.; ROZA, L.; SILVA, M. L. B. da; ALBINO, L..
2016
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Broilers; Microalgae; Digestibility; Calcium; Phosphorus.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1045621
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Carbon Dioxide Biofixation and Production of Spirulina sp. LEB 18 Biomass with Different Concentrations of NaNO3 and NaCl BABT
Moraes,Luiza; Rosa,Gabriel Martins da; Souza,Michele da Rosa Andrade Zimmermann de; Costa,Jorge Alberto Vieira.
ABSTRACT Microalgae are efficient at using solar energy to turn CO2 and nutrients into biomass containing lipids, proteins, carbohydrates and other compounds that may be used to produce bioproducts for human and animal consumption and pharmaceutical use. The aim of this study was to assess the effect of the NaNO3 and NaCl concentration on the growth kinetics, the biomass composition and the ability to biofix CO2 using the microalga Spirulina sp. LEB 18. The assays were carried out according to a 22 central composite design (CCD) with different concentrations of NaNO3 (1.25, 1.88 and 2.50 g L-1) and NaCl (1.00, 15.0 and 30.0 g L-1). The assays were carried out in 2 L vertical tubular photobioreactors at 30°C, 12 h light/dark and an injection of 12.0% v/v of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CO2; Fixation; Growth kinetics; Microalgae; Nutrients.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100410
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Cassava processing wastewater as a platform for third generation biodiesel production Scientia Agricola
Neves,Cristina; Maroneze,Mariana Manzoni; Santos,Aline Meireles dos; Francisco,Erika Cristina; Wagner,Roger; Zepka,Leila Queiroz; Jacob-Lopes,Eduardo.
ABSTRACT This study aimed to evaluate third generation biodiesel production by microalgae Phormidium autumnale using cassava processing wastewater as a platform. Experiments were performed in a heterotrophic bubble column bioreactor. The study focused on the evaluation of the bioreactor (batch and fed-batch) of different operational modes and the analysis of biofuel quality. Results indicate that fed-batch cultivations improved system performance, elevating biomass and oil productions to 12.0 g L−1 and 1.19 g L−1, respectively. The composition of this oil is predominantly saturated (60 %) and monounsaturated (39 %), resulting in a biodiesel that complys with U.S., European and Brazilian standards. The technological route developed indicates potential for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microalgae; Cyanobacteria; Agroindustrial wastewater; Biofuel.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000500412
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Characterization of phototrophic microorganisms and description of new cyanobacteria isolated from the saline-alkaline crater-lake Dziani Dzaha (Mayotte, Indian Ocean) ArchiMer
Cellamare, M.; Duval, C.; Drelin, Y.; Djediat, C.; Touibi, N.; Agogue, H.; Leboulanger, C.; Ader, M.; Bernard, C.
The saline-alkaline crater-lake Dziani Dzaha (Mayotte, Indian Ocean) is dominated by the bloom-forming cyanobacterium Arthrospira. However, the rest of the phototrophic community remains underexplored because of their minute dimension or lower biomass. To characterize the phototrophic microorganisms living in this ecosystem considered as a modern analog of Precambrian environments, several strains were isolated from the water column and stromatolites, and analyzed using the polyphasic approach. Based on morphological, ultrastructural and molecular (16S rRNA gene, 18S rRNA gene, 16S-23S ITS region, cpcBA-IGS locus) methods, seven filamentous cyanobacteria and the prasinophyte Picocystis salinarum were identified. Two new genera and four new cyanobacteria...
Tipo: Text Palavras-chave: Cyanobacteria; Microalgae; Crater-lake; Saline-alkaline lake; Polyphasic approach; Arthrospira.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00443/55469/56991.pdf
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Combined Effects of Nitrogen Concentration and Seasonal Changes on the Production of Lipids in Nannochloropsis oculata ArchiMer
Olofsson, Martin; Lamela, Teresa; Nilsson, Emmelie; Berge, Jean-pascal; Del Pino, Victoria; Uronen, Pauliina; Legrand, Catherine.
Instead of sole nutrient starvation to boost algal lipid production, we addressed nutrient limitation at two different seasons (autumn and spring) during outdoor cultivation in flat panel photobioreactors. Lipid accumulation, biomass and lipid productivity and changes in fatty acid composition of Nannochloropsis oculata were investigated under nitrogen (N) limitation (nitrate:phosphate N:P 5, N:P 2.5 molar ratio). N. oculata was able to maintain a high biomass productivity under N-limitation compared to N-sufficiency (N:P 20) at both seasons, which in spring resulted in nearly double lipid productivity under N-limited conditions (0.21 g L−1 day−1) compared to N-sufficiency (0.11 g L−1 day−1). Saturated and monounsaturated fatty acids increased from 76% to...
Tipo: Text Palavras-chave: Microalgae; Outdoor; Nannochloropsis; Large-scale; Lipids; Fatty acids; Nitrogen manipulation; Seasonal changes; Biofuels; High value products.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00184/29564/27902.pdf
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Comparative proteomics reveals proteins impacted by nitrogen deprivation in wild-type and high lipid-accumulating mutant strains of Tisochrysis lutea ArchiMer
Garnier, Matthieu; Carrier, Gregory; Rogniaux, Helene; Nicolau, Elodie; Bougaran, Gael; Saint-jean, Bruno; Cadoret, Jean-paul.
Understanding microalgal lipid accumulation under nitrogen starvation is of major interest for biomass feedstock, food and biofuel production. Using a domesticated oleaginous algae Tisochrysis lutea, we performed the first comparative proteomic analysis on the wild type strain and a selected lipid over-accumulating mutant. 2-DE analysis was made on these strains cultured in two metabolic conditions, with and without nitrogen deprivation, which revealed significant differences in proteomes according to both strain and nitrogen availability. Mass spectrometry allowed us to identify 37 proteins that were differentially expressed between the two strains, and 17 proteins regulated by nitrogen starvation concomitantly with lipid accumulation. The proteins...
Tipo: Text Palavras-chave: Microalgae; Biotechnology; Lipid; Nitrogen; Proteomic; Selection.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00179/29012/27470.pdf
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Compte-rendu atelier phyto du grand ouest 2018 ArchiMer
Legendre, Aurelie; Neaud-masson, Nadine.
Phytoplankton analysts from the North and West coastal regions of France met together for the 5th edition of the phytoplankton workshop between 12 - 13 April 2018 in Dinard. During these two days, the analysts could share the challenges they face regarding the identification of some species and about the development of nomenclature as well.
Tipo: Text Palavras-chave: Phytoplancton; Microalgue; Taxinomie; Identification / Phytoplankton; Microalgae; Taxonomy; Identification.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00487/59870/63043.pdf
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Cultivation of Nannochloropsis sp. in brackish groundwater supplemented with municipal wastewater as a nutrient source BABT
Sousa,Louise Lins de; Hora,Dominique Silveira da; Sales,Emerson Andrade; Perelo,Louisa Wessels.
The aim of this work was to study growth potential of the green microalgae Nannochloropsis sp. using brackish groundwater from a well in the semi-arid northeast region of Brazil as culture medium. The medium was supplemented with (%) 19.4, 22.0, 44.0 and 50.0% of municipal wastewater after UASB treatment as a low-cost nutrient source. The results showed that the culture tested was capable of growing in the brackish groundwater even at salinity levels as low as 2 ppt. Furthermore it was shown that municipal wastewater could be used as a sole nutrient source for Nannochloropsis sp.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microalgae; Brackish groundwater; Municipal wastewater; Nannochloropsis sp.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200003
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Determination of microalgae cellular composition after phycoremediation of swine wastewaters. Repositório Alice
MICHELON, W.; DA SILVA, M. L. B.; MEZZARI, M. P.; PIROLLI, M.; PRANDINI, J.; SOARES, H. M..
Phycoremediation of swine wastewaters has been widely reported as an attractive tertiary treatment system, that effectively removes the excessive nutrient loadswhilst offering a valuable source of feedstock biomass. Digestate from an upflow anaerobic sludge blanket (UASB, 6%v/v) and a nitrification reactor (NR; 50% v/v) were used as culturing media to microalgae. Experiments were carried out in lab scale photobioreactors (PBRs) using a consortia of Chlorella and Scenedesmus. Ammonia (44 to 90%) and phosphorus (77%) were efficiently removed from both effluents tested after 4 days. Microalgae biomass harvested from the UASB effluent showed 57, 34 and 1% of proteins, carbohydrates and lipids, respectively. Comparatively, the cellular composition of microalgae...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Carbohydrate; Lipid; Microalgae; Protein; Phycoremediation.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1045552
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