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Registros recuperados: 10
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Atividade enzimática e perfil da comunidade bacteriana em solo submetido à solarização e biofumigação. Repositório Alice
PASSOS, S.R.; REIS JUNIOR, F.B. dos; RUMJANEK, N.G.; MENDES, I. de C.; BAPTISTA, M.J.; XAVIER, G.R..
O objetivo deste trabalho foi avaliar o efeito da solarização e da biofumigação sobre a comunidade microbiana do solo, por meio da atividade da enzima beta-glicosidase e do perfil do 16S rDNA, determinado com PCR-DGGE. A solarização do solo, com cobertura de plástico, foi feita por períodos de dois, quatro e seis meses, e a biofumigação foi realizada pela incorporação de 2 e 5% (v/v) de cama-de-frango ao solo. Logo após a retirada da cobertura de plástico e aos 30 dias após a remoção, a atividade da beta-glicosidase foi menor em relação ao tratamento não solarizado. Aos 60 dias, não foram mais observadas diferenças entre os tratamentos. A adição de cama-de-frango a 5% estimulou a atividade da beta-glicosidase. O perfil da estrutura da comunidade bacteriana...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Beta-glicosidase; Cama-de-frango; Diversidade microbiana; Ecologia microbiana; Manejo do solo; 16S rDNA; Chicken litter; Microbial diversity; Microbial ecology; Soil management.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/122109
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Atividade enzimática e perfil da comunidade bacteriana em solos submetido à solarização e biofumigação. Repositório Alice
PASSOS, S. R.; REIS JÚNIOR, F. B. dos; RUMJANEK, N. G.; MENDES, I. de C.; BAPTISTA, M. J.; XAVIER, G. R..
O objetivo deste trabalho foi avaliar o efeito da solarização e da biofumigação sobre a comunidade microbiana do solo, por meio da atividade da enzima beta-glicosidase e do perfil do 16S rDNA, determinado com PCR-DGGE. A solarização do solo, com cobertura de plástico, foi feita por períodos de dois, quatro e seis meses, e a biofumigação foi realizada pela incorporação de 2 e 5% (v/v) de cama-de-frango ao solo. Logo após a retirada da cobertura de plástico e aos 30 dias após a remoção, a atividade da beta-glicosidase foi menor em relação ao tratamento não solarizado. Aos 60 dias, não foram mais observadas diferenças entre os tratamentos. A adição de cama-de-frango a 5% estimulou a atividade da beta-glicosidase. O perfil da estrutura da comunidade bacteriana...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Solarização; Beta-glicosidase; Cama-de-frango; Diversidade microbiana; Ecologia microbiana; Manejo do solo; Solarization; Beta-glucosidase; Chicken litter; Microbial diversity; Microbial ecology; Soil management.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/630059
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Comparative Study of Guaymas Basin Microbiomes: Cold Seeps vs. Hydrothermal Vents Sediments ArchiMer
Cruaud, Perrine; Vigneron, Adrien; Pignet, Patricia; Caprais, Jean-claude; Lesongeur, Francoise; Toffin, Laurent; Godfroy, Anne; Cambon-bonavita, Marie-anne.
In the Guaymas Basin, the presence at a few tens of kilometers of cold seeps and hydrothermal vents coupled with comparable sedimentary settings and depths offer a unique opportunity to assess and compare the microbial community composition of these deep-sea ecosystems. The microbial diversity in sediments from three cold seep and two hydrothermal vent assemblages were investigated using high-throughput 16S rRNA-sequencing. Numerous bacterial and archaeal lineages were detected in both cold seep and hydrothermal vent sediments. Various potential organic matter degraders (e.g., Chloroflexi, Atribacteria, MBG-D) and methane and sulfur cycling related microorganisms (e.g., ANME and methanogenic lineages, sulfate-reducing lineages) were detected in both...
Tipo: Text Palavras-chave: Microbial diversity; Hydrothermal vents; Cold seeps; Ribotypes; Methane; Extreme environments.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00415/52640/53496.pdf
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Continuous enrichment cultures: insights into prokaryotic diversity and metabolic interactions in deep-sea vent chimneys ArchiMer
Postec, Anne; Lesongeur, Francoise; Pignet, Patricia; Ollivier, B; Querellou, Joel; Godfroy, Anne.
The prokaryotic diversity of culturable thermophilic communities of deep-sea hydrothermal chimneys was analysed using a continuous enrichment culture performed in a gas-lift bioreactor, and compared to classical batch enrichment cultures in vials. Cultures were conducted at 60 degrees C and pH 6.5 using a complex medium containing carbohydrates, peptides and sulphur, and inoculated with a sample of a hydrothermal black chimney collected at the Rainbow field, Mid-Atlantic Ridge, at 2,275 m depth. To assess the relevance of both culture methods, bacterial and archaeal diversity was studied using cloning and sequencing, DGGE, and whole-cell hybridisation of 16S rRNA genes. Sequences of heterotrophic microorganisms belonging to the genera Marinitoga,...
Tipo: Text Palavras-chave: Metabolic interactions; 16S rRNA gene; Thermophiles; Bioreactor; Continuous enrichment cultures; Deep sea hydrothermal vent; Microbial diversity.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3885.pdf
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Effect of variation of environmental conditions on the microbial communities of deep-sea vent chimneys, cultured in a bioreactor ArchiMer
Byrne, Nathalie; Lesongeur, Francoise; Bienvenu, Nadege; Geslin, Claire; Alain, Karine; Prieur, Daniel; Godfroy, Anne.
Both cultivation and molecular techniques were used to investigate the microbial diversity and dynamic of a deep-sea vent chimney. The enrichment cultures performed in a gas-lift bioreactor were inoculated with a black smoker chimney sample collected on TAG site on the mid-Atlantic ridge. To mimic as close as possible environmental conditions, the cultures were performed in oligotrophic medium with nitrogen, hydrogen and carbon dioxide (N-2/H-2/CO2) gas sweeping. Also, the temperature was first settled at a temperature of 85A degrees C and colloidal sulphur was added. Then, the temperature was lowered to 60A degrees C and sulphur was omitted. Archaeal and bacterial diversity was studied in both culture and natural samples. Through 16S rRNA gene sequences...
Tipo: Text Palavras-chave: 16S rRNA genes; Oligotrophic condition; Environmental condition; Bioreactor; Continuous enrichment culture; Microbial diversity; Deep sea hydrothermal vent.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6920.pdf
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Isolamento e caracterização morfofisiológica de fungos filamentosos de áreas mineradas. Repositório Alice
BATISTA, A. M.; MATTOS, B. B.; SANTOS, S. S.; OLIVEIRA, C. A.; OLIVEIRA, M. C. R.; TAKAHASHI, J. A.; SOUZA, F. A.; MARRIEL, I. E..
2014
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Área degradada; Morfofisiologia; Diversidade microbiana; Degraded areas; Morphophysiology; Microbial diversity.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1001577
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Microbial communities and their biogeochemical role in the water column of the oxygen minimum zone in the eastern South Pacific Gayana
Ulloa,Osvaldo; Belmar,Lucy; Farías,Laura; Castro-González,Maribel; Galán,Alexander; Lavín,Paris; Molina,Verónica; Ramírez,Salvador; Santibáñez,Francisco; Stevens,Heike.
In recent years, our group has been studying the microbial communities of the water column associated with the oxygen minimum zone (OMZ) off northern Chile and Peru, trying to understand who the main players are in the OMZ biogeochemical cycles and which metabolic strategies they are using. For that, we are combining flow cytometry, molecular techniques and biogeochemical approaches. Most of our work, so far, has focused on microbes capable of doing oxygenic photosynthesis, such as the cyanobacteria, and those involved in the nitrogen cycle, such as the denitrifying, nitrifying and anammox bacteria. We have also started to look at the more general microbial abundance and diversity. In each case, we are finding that the OMZ holds distinct microbial...
Tipo: Journal article Palavras-chave: Oxygen minimum zone; Microbial diversity; Nitrogen cycle.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382006000300018
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Microbial diversity associated to the intestinal tract of soil invertebrates. Repositório Alice
CORREIA, D. da S.; PASSOS, S. R.; PROENÇA, D. N.; MORAIS, P. V.; XAVIER, G. R.; CORREIA, M. E. F..
Interactions between saprophagous invertebrates and microbes are essential for the maintenance and functioning of soil ecosystems, as they directly affect the degradation of organic matter and the nutrient cycle. The intestinal tract of invertebrates is inhabited by a diversity of microbes, and it is closely associated with the food ingested. The aim of this work was to evaluate the profile of prokaryotes associated with the intestinal tract of three invertebrate species. The species of invertebrates Trigoniulus corallinus was collected and incubated in the experiment, after 5 days of incubation we observed the uninduced colonization of two invertebrate species Cubaris murina and Pycnoscelus surinamensis.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Saprophagous; DGGE analysis; Microbial diversity; Invertebrates.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1098179
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Phylogenetic characterization of the bacterial assemblage associated with mucous. secretions of the hydrothermal vent polychaete Paralvinella palmiformis ArchiMer
Alain, Karine; Olagnon, Michel; Desbruyeres, Daniel; Page, Antoine; Barbier, Georges; Juniper, Kim; Querellou, Joel; Cambon-bonavita, Marie-anne.
As part of an ongoing examination of microbial diversity associated with hydrothermal vent polychaetes of the family Alvinellidae, we undertook a culture-independent molecular analysis of the bacterial assemblage associated with mucous secretions of the Northeastern Pacific vent polychaete Paralvinella palmiformis. Using a molecular 16S rDNA-based phylogenetic approach, clone libraries were constructed from two samples collected from active sulfide edifices in two hydrothermal vent fields. In both cases, clone libraries were largely dominated by epsilon-Proteobacteria. Phylotypes belonging to the Cytophaga-Flavobacteria and to the Verrucomicrobia were also largely represented within the libraries. The remaining sequences were related to the taxonomic...
Tipo: Text Palavras-chave: Proteobacteria; 16S rRNA; Microbial diversity; Phylogeny; Hydrothermal vent; Paralvinella palmiformis.
Ano: 2002 URL: https://archimer.ifremer.fr/doc/2002/publication-661.pdf
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THE HUMAN MICROBIOTA: THE ROLE OF MICROBIAL COMMUNITIES IN HEALTH AND DISEASE Acta biol.Colomb.
BOTERO,Luz Elena; DELGADO-SERRANO,Luisa; CEPEDA HERNÁNDEZ,Martha Lucía; DEL PORTILLO OBANDO,Patricia; ZAMBRANO EDER,María Mercedes.
During the last decade, there has been increasing awareness of the massive number of microorganisms, collectively known as the human microbiota, that are associated with humans. This microbiota outnumbers the host cells by approximately a factor of ten and contains a large repertoire of microbial genome-encoded metabolic processes. The diverse human microbiota and its associated metabolic potential can provide the host with novel functions that can influence host health and disease status in ways that still need to be analyzed. The microbiota varies with age, with features that depend on the body site, host lifestyle and health status. The challenge is therefore to identify and characterize these microbial communities and use this information to learn how...
Tipo: Journal article Palavras-chave: 16S rRNA gene; Microbial diversity; Microbiome; Mycobacterium tuberculosis.
Ano: 2016 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2016000100001
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